Therapeutic concentration of morphine reduces oxidative stress in glioma cell line
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/6630 |
Resumo: | Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and levels of sulfhydryl groups (an indicator of the redox state of the cell). Morphine did not prevent the decrease in cell viability provoked by H2O2) but partially prevented lipid peroxidation caused by 0.0025% H2O2) (a concentration allowing more than 90% cell viability). Interestingly, this opioid did not alter the increased levels of sulfhydryl groups produced by exposure to 0.0025% H2O2), opening the possibility that alternative molecular mechanisms (a direct scavenging activity or the inhibition of NAPDH oxidase) may explain the protective effect registered in the lipid peroxidation assay. Our results demonstrate, for the first time, that morphine in usual analgesic doses may contribute to minimizing oxidative stress in cells of glial origin. This study supports the importance of employing concentrations similar to those used in clinical practice for a better approximation between experimental models and the clinical setting. |
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2015-05-08T14:09:59Z2015-05-08T14:09:59Z2014-05ALMEIDA, M. B. et al. Therapeutic concentration of morphine reduces oxidative stress in glioma cell line. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 47, n. 5, p. 398-402, maio 2014. Disponível em: <http://www.scielo.br/pdf/bjmbr/v47n5/1414-431X-bjmbr-1414-431X20143697.pdf>. Acesso em: 23 abr. 2015. <http://dx.doi.org/10.1590/1414-431X20143697>.1414-431Xhttp://repositorio.ufpa.br/jspui/handle/2011/6630Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and levels of sulfhydryl groups (an indicator of the redox state of the cell). Morphine did not prevent the decrease in cell viability provoked by H2O2) but partially prevented lipid peroxidation caused by 0.0025% H2O2) (a concentration allowing more than 90% cell viability). Interestingly, this opioid did not alter the increased levels of sulfhydryl groups produced by exposure to 0.0025% H2O2), opening the possibility that alternative molecular mechanisms (a direct scavenging activity or the inhibition of NAPDH oxidase) may explain the protective effect registered in the lipid peroxidation assay. Our results demonstrate, for the first time, that morphine in usual analgesic doses may contribute to minimizing oxidative stress in cells of glial origin. This study supports the importance of employing concentrations similar to those used in clinical practice for a better approximation between experimental models and the clinical setting.engMorfinaNeurogliaEstresse oxidativoPeróxido de hidrogênioPeroxidação de lipídeosAnalgésicos opioidesTerapêuticaTherapeutic concentration of morphine reduces oxidative stress in glioma cell lineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleALMEIDA, Mauro Brito deMALAQUIAS, Allan CostaNASCIMENTO, José Luiz Martins doOLIVEIRA, Karen Renata MatosSILVA, Anderson Manoel Herculano Oliveira daCRESPO LÓPEZ, Maria Elenainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPACC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/6630/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-822762http://repositorio.ufpa.br/oai/bitstream/2011/6630/3/license_text321e754e6058db75d1137df814352636MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-822190http://repositorio.ufpa.br/oai/bitstream/2011/6630/4/license_rdf19e8a2b57ef43c09f4d7071d2153c97dMD54ORIGINALArtigo_TherapeuticConcentrationMorphine.pdfArtigo_TherapeuticConcentrationMorphine.pdfapplication/pdf226366http://repositorio.ufpa.br/oai/bitstream/2011/6630/1/Artigo_TherapeuticConcentrationMorphine.pdf29c95b20eac9a93ab7765c29205d8708MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81703http://repositorio.ufpa.br/oai/bitstream/2011/6630/5/license.txta12ee01655d4f43dacf016d5e6168febMD55TEXTArtigo_TherapeuticConcentrationMorphine.pdf.txtArtigo_TherapeuticConcentrationMorphine.pdf.txtExtracted texttext/plain24186http://repositorio.ufpa.br/oai/bitstream/2011/6630/6/Artigo_TherapeuticConcentrationMorphine.pdf.txtd6f5c92268ee9ec9e93decd909959dd5MD562011/66302018-01-10 13:13:59.29oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232018-01-10T16:13:59Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
title |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
spellingShingle |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line ALMEIDA, Mauro Brito de Morfina Neuroglia Estresse oxidativo Peróxido de hidrogênio Peroxidação de lipídeos Analgésicos opioides Terapêutica |
title_short |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
title_full |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
title_fullStr |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
title_full_unstemmed |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
title_sort |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
author |
ALMEIDA, Mauro Brito de |
author_facet |
ALMEIDA, Mauro Brito de MALAQUIAS, Allan Costa NASCIMENTO, José Luiz Martins do OLIVEIRA, Karen Renata Matos SILVA, Anderson Manoel Herculano Oliveira da CRESPO LÓPEZ, Maria Elena |
author_role |
author |
author2 |
MALAQUIAS, Allan Costa NASCIMENTO, José Luiz Martins do OLIVEIRA, Karen Renata Matos SILVA, Anderson Manoel Herculano Oliveira da CRESPO LÓPEZ, Maria Elena |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
ALMEIDA, Mauro Brito de MALAQUIAS, Allan Costa NASCIMENTO, José Luiz Martins do OLIVEIRA, Karen Renata Matos SILVA, Anderson Manoel Herculano Oliveira da CRESPO LÓPEZ, Maria Elena |
dc.subject.por.fl_str_mv |
Morfina Neuroglia Estresse oxidativo Peróxido de hidrogênio Peroxidação de lipídeos Analgésicos opioides Terapêutica |
topic |
Morfina Neuroglia Estresse oxidativo Peróxido de hidrogênio Peroxidação de lipídeos Analgésicos opioides Terapêutica |
description |
Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and levels of sulfhydryl groups (an indicator of the redox state of the cell). Morphine did not prevent the decrease in cell viability provoked by H2O2) but partially prevented lipid peroxidation caused by 0.0025% H2O2) (a concentration allowing more than 90% cell viability). Interestingly, this opioid did not alter the increased levels of sulfhydryl groups produced by exposure to 0.0025% H2O2), opening the possibility that alternative molecular mechanisms (a direct scavenging activity or the inhibition of NAPDH oxidase) may explain the protective effect registered in the lipid peroxidation assay. Our results demonstrate, for the first time, that morphine in usual analgesic doses may contribute to minimizing oxidative stress in cells of glial origin. This study supports the importance of employing concentrations similar to those used in clinical practice for a better approximation between experimental models and the clinical setting. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-05 |
dc.date.accessioned.fl_str_mv |
2015-05-08T14:09:59Z |
dc.date.available.fl_str_mv |
2015-05-08T14:09:59Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALMEIDA, M. B. et al. Therapeutic concentration of morphine reduces oxidative stress in glioma cell line. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 47, n. 5, p. 398-402, maio 2014. Disponível em: <http://www.scielo.br/pdf/bjmbr/v47n5/1414-431X-bjmbr-1414-431X20143697.pdf>. Acesso em: 23 abr. 2015. <http://dx.doi.org/10.1590/1414-431X20143697>. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/6630 |
dc.identifier.issn.none.fl_str_mv |
1414-431X |
identifier_str_mv |
ALMEIDA, M. B. et al. Therapeutic concentration of morphine reduces oxidative stress in glioma cell line. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 47, n. 5, p. 398-402, maio 2014. Disponível em: <http://www.scielo.br/pdf/bjmbr/v47n5/1414-431X-bjmbr-1414-431X20143697.pdf>. Acesso em: 23 abr. 2015. <http://dx.doi.org/10.1590/1414-431X20143697>. 1414-431X |
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http://repositorio.ufpa.br/jspui/handle/2011/6630 |
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