Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia

• Autor(a) principal: QUEIROZ, Maria Alice Freitas
• Data de Publicação: 2016
• Outros Autores: GOMES, Samara Tatielle Monteiro, ALMEIDA, Núbia Caroline Costa de, SOUSA, Maria Izete Machado de, COSTA, Suzanne Roberta Cardoso Fernandes, HERMES, Renata Bezerra, LIMA, Sandra Souza, ZANINOTTO, Marcelo Martins, FOSSA, Marco Antonio Ayin , MANESCYH, Manoel Araujo, FEITOSA, Rosimar Neris Martins, AZEVEDO, Vânia Nakauth, MACHADO, Luiz Fernando Almeida, ISHAK, Marluísa de Oliveira Guimarães, ISHAK, Ricardo, VALLINOTO, Antonio Carlos Rosário
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UFPA
• Texto Completo: http://repositorio.ufpa.br/jspui/handle/2011/8375
• topic: Polimofismo nos genes; Lectinas de ligação a manose; Proteínas; Infecções por Chlamydia

The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia.