Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia

Detalhes bibliográficos
Autor(a) principal: QUEIROZ, Maria Alice Freitas
Data de Publicação: 2016
Outros Autores: GOMES, Samara Tatielle Monteiro, ALMEIDA, Núbia Caroline Costa de, SOUSA, Maria Izete Machado de, COSTA, Suzanne Roberta Cardoso Fernandes, HERMES, Renata Bezerra, LIMA, Sandra Souza, ZANINOTTO, Marcelo Martins, FOSSA, Marco Antonio Ayin , MANESCYH, Manoel Araujo, FEITOSA, Rosimar Neris Martins, AZEVEDO, Vânia Nakauth, MACHADO, Luiz Fernando Almeida, ISHAK, Marluísa de Oliveira Guimarães, ISHAK, Ricardo, VALLINOTO, Antonio Carlos Rosário
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFPA
Texto Completo: http://repositorio.ufpa.br/jspui/handle/2011/8375
Resumo: The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia.
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spelling 2017-05-12T14:25:50Z2017-05-12T14:25:50Z2016-12QUEIROZ, Maria Alice Freitas et al. Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 49, n. 12, e5519, 2016. Disponível em:<http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200604&lng=pt&nrm=iso>. Acesso em: 12 maio 2017. Epub 12-Dez-2016. <http://dx.doi.org/10.1590/1414-431x20165519>.1414-431Xhttp://repositorio.ufpa.br/jspui/handle/2011/8375The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia.porUniversidade Federal do ParáUFPABrasilBrazilian Journal of Medical and Biological ResearchPolimofismo nos genesLectinas de ligação a manoseProteínasInfecções por ChlamydiaMannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article491219QUEIROZ, Maria Alice FreitasGOMES, Samara Tatielle MonteiroALMEIDA, Núbia Caroline Costa deSOUSA, Maria Izete Machado deCOSTA, Suzanne Roberta Cardoso FernandesHERMES, Renata BezerraLIMA, Sandra SouzaZANINOTTO, Marcelo MartinsFOSSA, Marco Antonio Ayin MANESCYH, Manoel AraujoFEITOSA, Rosimar Neris MartinsAZEVEDO, Vânia NakauthMACHADO, Luiz Fernando AlmeidaISHAK, Marluísa de Oliveira GuimarãesISHAK, RicardoVALLINOTO, Antonio Carlos Rosárioinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPATEXTArtigo_MannoseBindingLectin.pdf.txtArtigo_MannoseBindingLectin.pdf.txtExtracted texttext/plain33602http://repositorio.ufpa.br/oai/bitstream/2011/8375/6/Artigo_MannoseBindingLectin.pdf.txt5031b8ad672791e2350e583d64948b53MD56ORIGINALArtigo_MannoseBindingLectin.pdfArtigo_MannoseBindingLectin.pdfapplication/pdf1575294http://repositorio.ufpa.br/oai/bitstream/2011/8375/1/Artigo_MannoseBindingLectin.pdfa570fe8a9121370f47d0b49a866cd1c0MD51CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.pt_BR.fl_str_mv Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia
title Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia
spellingShingle Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia
QUEIROZ, Maria Alice Freitas
Polimofismo nos genes
Lectinas de ligação a manose
Proteínas
Infecções por Chlamydia
title_short Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia
title_full Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia
title_fullStr Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia
title_full_unstemmed Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia
title_sort Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia
author QUEIROZ, Maria Alice Freitas
author_facet QUEIROZ, Maria Alice Freitas
GOMES, Samara Tatielle Monteiro
ALMEIDA, Núbia Caroline Costa de
SOUSA, Maria Izete Machado de
COSTA, Suzanne Roberta Cardoso Fernandes
HERMES, Renata Bezerra
LIMA, Sandra Souza
ZANINOTTO, Marcelo Martins
FOSSA, Marco Antonio Ayin 
MANESCYH, Manoel Araujo
FEITOSA, Rosimar Neris Martins
AZEVEDO, Vânia Nakauth
MACHADO, Luiz Fernando Almeida
ISHAK, Marluísa de Oliveira Guimarães
ISHAK, Ricardo
VALLINOTO, Antonio Carlos Rosário
author_role author
author2 GOMES, Samara Tatielle Monteiro
ALMEIDA, Núbia Caroline Costa de
SOUSA, Maria Izete Machado de
COSTA, Suzanne Roberta Cardoso Fernandes
HERMES, Renata Bezerra
LIMA, Sandra Souza
ZANINOTTO, Marcelo Martins
FOSSA, Marco Antonio Ayin 
MANESCYH, Manoel Araujo
FEITOSA, Rosimar Neris Martins
AZEVEDO, Vânia Nakauth
MACHADO, Luiz Fernando Almeida
ISHAK, Marluísa de Oliveira Guimarães
ISHAK, Ricardo
VALLINOTO, Antonio Carlos Rosário
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv QUEIROZ, Maria Alice Freitas
GOMES, Samara Tatielle Monteiro
ALMEIDA, Núbia Caroline Costa de
SOUSA, Maria Izete Machado de
COSTA, Suzanne Roberta Cardoso Fernandes
HERMES, Renata Bezerra
LIMA, Sandra Souza
ZANINOTTO, Marcelo Martins
FOSSA, Marco Antonio Ayin 
MANESCYH, Manoel Araujo
FEITOSA, Rosimar Neris Martins
AZEVEDO, Vânia Nakauth
MACHADO, Luiz Fernando Almeida
ISHAK, Marluísa de Oliveira Guimarães
ISHAK, Ricardo
VALLINOTO, Antonio Carlos Rosário
dc.subject.por.fl_str_mv Polimofismo nos genes
Lectinas de ligação a manose
Proteínas
Infecções por Chlamydia
topic Polimofismo nos genes
Lectinas de ligação a manose
Proteínas
Infecções por Chlamydia
description The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia.
publishDate 2016
dc.date.issued.fl_str_mv 2016-12
dc.date.accessioned.fl_str_mv 2017-05-12T14:25:50Z
dc.date.available.fl_str_mv 2017-05-12T14:25:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv QUEIROZ, Maria Alice Freitas et al. Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 49, n. 12, e5519, 2016. Disponível em:<http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200604&lng=pt&nrm=iso>. Acesso em: 12 maio 2017. Epub 12-Dez-2016. <http://dx.doi.org/10.1590/1414-431x20165519>.
dc.identifier.uri.fl_str_mv http://repositorio.ufpa.br/jspui/handle/2011/8375
dc.identifier.issn.pt_BR.fl_str_mv 1414-431X
identifier_str_mv QUEIROZ, Maria Alice Freitas et al. Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 49, n. 12, e5519, 2016. Disponível em:<http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200604&lng=pt&nrm=iso>. Acesso em: 12 maio 2017. Epub 12-Dez-2016. <http://dx.doi.org/10.1590/1414-431x20165519>.
1414-431X
url http://repositorio.ufpa.br/jspui/handle/2011/8375
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dc.relation.ispartof.pt_BR.fl_str_mv Brazilian Journal of Medical and Biological Research
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dc.publisher.none.fl_str_mv Universidade Federal do Pará
dc.publisher.initials.fl_str_mv UFPA
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