Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus)

Detalhes bibliográficos
Autor(a) principal: HENRIQUES, Daniele Freitas
Data de Publicação: 2012
Outros Autores: QUARESMA, Juarez Antônio Simões, FUZII, Hellen Thais, NUNES, Márcio Roberto Teixeira, SILVA, Eliana Vieira Pinto da, CARVALHO, Valéria Lima, MARTINS, Lívia Carício, CASSEB, Samir Mansour Moraes, CHIANG, Jannifer Oliveira, VASCONCELOS, Pedro Fernando da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFPA
Texto Completo: http://repositorio.ufpa.br/jspui/handle/2011/4119
Resumo: Rocio virus (ROCV) is an encephalitic flavivirus endemic to Brazil. Experimental flavivirus infections have previously demonstrated a persistent infection and, in this study, we investigated the persistence of ROCV infection in golden hamsters (Mesocricetus auratus). The hamsters were infected intraperitoneally with 9.8 LD50/0.02 mL of ROCV and later anaesthetised and sacrificed at various time points over a 120-day period to collect of blood, urine and organ samples. The viral titres were quantified by real-time-polymerase chain reaction (qRT-PCR). The specimens were used to infect Vero cells and ROCV antigens in the cells were detected by immunefluorescence assay. The levels of antibodies were determined by the haemagglutination inhibition technique. A histopathological examination was performed on the tissues by staining with haematoxylin-eosin and detecting viral antigens by immunohistochemistry (IHC). ROCV induced a strong immune response and was pathogenic in hamsters through neuroinvasion. ROCV was recovered from Vero cells exposed to samples from the viscera, brain, blood, serum and urine and was detected by qRT-PCR in the brain, liver and blood for three months after infection. ROCV induced histopathological changes and the expression of viral antigens, which were detected by IHC in the liver, kidney, lung and brain up to four months after infection. These findings show that ROCV is pathogenic to golden hamsters and has the capacity to cause persistent infection in animals after intraperitoneal infection.
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spelling 2013-08-13T13:28:42Z2013-08-13T13:28:42Z2012-08HENRIQUES, Daniele Freitas, et al. Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 5, p. 630-636, ago. 2012. Disponível em: <http://www.scielo.br/pdf/mioc/v107n5/09.pdf>. Acesso em: 17 jul. 2013. <http://dx.doi.org/10.1590/S0074-02762012000500009>.1678-80600074-0276http://repositorio.ufpa.br/jspui/handle/2011/4119Rocio virus (ROCV) is an encephalitic flavivirus endemic to Brazil. Experimental flavivirus infections have previously demonstrated a persistent infection and, in this study, we investigated the persistence of ROCV infection in golden hamsters (Mesocricetus auratus). The hamsters were infected intraperitoneally with 9.8 LD50/0.02 mL of ROCV and later anaesthetised and sacrificed at various time points over a 120-day period to collect of blood, urine and organ samples. The viral titres were quantified by real-time-polymerase chain reaction (qRT-PCR). The specimens were used to infect Vero cells and ROCV antigens in the cells were detected by immunefluorescence assay. The levels of antibodies were determined by the haemagglutination inhibition technique. A histopathological examination was performed on the tissues by staining with haematoxylin-eosin and detecting viral antigens by immunohistochemistry (IHC). ROCV induced a strong immune response and was pathogenic in hamsters through neuroinvasion. ROCV was recovered from Vero cells exposed to samples from the viscera, brain, blood, serum and urine and was detected by qRT-PCR in the brain, liver and blood for three months after infection. ROCV induced histopathological changes and the expression of viral antigens, which were detected by IHC in the liver, kidney, lung and brain up to four months after infection. These findings show that ROCV is pathogenic to golden hamsters and has the capacity to cause persistent infection in animals after intraperitoneal infection.engFlavivírusVírus RocioInfecção experimentalPersistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHENRIQUES, Daniele FreitasQUARESMA, Juarez Antônio SimõesFUZII, Hellen ThaisNUNES, Márcio Roberto TeixeiraSILVA, Eliana Vieira Pinto daCARVALHO, Valéria LimaMARTINS, Lívia CarícioCASSEB, Samir Mansour MoraesCHIANG, Jannifer OliveiraVASCONCELOS, Pedro Fernando da Costainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_PersistenceExperimentalRocio.pdfArtigo_PersistenceExperimentalRocio.pdfapplication/pdf1670322http://repositorio.ufpa.br/oai/bitstream/2011/4119/1/Artigo_PersistenceExperimentalRocio.pdf276c89a48eac4acf0103b3659cb5a2ccMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/4119/2/license_urlfd26723f8d7edacdb29e3f03465c3b03MD52license_textlicense_texttext/html; charset=utf-80http://repositorio.ufpa.br/oai/bitstream/2011/4119/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-823599http://repositorio.ufpa.br/oai/bitstream/2011/4119/4/license_rdf9e2b7f6edbd693264102b96ece20428aMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81996http://repositorio.ufpa.br/oai/bitstream/2011/4119/5/license.txt4b350777eea7a4b1ae7cd9a06fd687a1MD55TEXTArtigo_PersistenceExperimentalRocio.pdf.txtArtigo_PersistenceExperimentalRocio.pdf.txtExtracted texttext/plain34099http://repositorio.ufpa.br/oai/bitstream/2011/4119/6/Artigo_PersistenceExperimentalRocio.pdf.txtaa401f3b586520ae6ddadd1293d2a5aeMD562011/41192018-01-25 13:30:10.623oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232018-01-25T16:30:10Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false
dc.title.pt_BR.fl_str_mv Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus)
title Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus)
spellingShingle Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus)
HENRIQUES, Daniele Freitas
Flavivírus
Vírus Rocio
Infecção experimental
title_short Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus)
title_full Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus)
title_fullStr Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus)
title_full_unstemmed Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus)
title_sort Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus)
author HENRIQUES, Daniele Freitas
author_facet HENRIQUES, Daniele Freitas
QUARESMA, Juarez Antônio Simões
FUZII, Hellen Thais
NUNES, Márcio Roberto Teixeira
SILVA, Eliana Vieira Pinto da
CARVALHO, Valéria Lima
MARTINS, Lívia Carício
CASSEB, Samir Mansour Moraes
CHIANG, Jannifer Oliveira
VASCONCELOS, Pedro Fernando da Costa
author_role author
author2 QUARESMA, Juarez Antônio Simões
FUZII, Hellen Thais
NUNES, Márcio Roberto Teixeira
SILVA, Eliana Vieira Pinto da
CARVALHO, Valéria Lima
MARTINS, Lívia Carício
CASSEB, Samir Mansour Moraes
CHIANG, Jannifer Oliveira
VASCONCELOS, Pedro Fernando da Costa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv HENRIQUES, Daniele Freitas
QUARESMA, Juarez Antônio Simões
FUZII, Hellen Thais
NUNES, Márcio Roberto Teixeira
SILVA, Eliana Vieira Pinto da
CARVALHO, Valéria Lima
MARTINS, Lívia Carício
CASSEB, Samir Mansour Moraes
CHIANG, Jannifer Oliveira
VASCONCELOS, Pedro Fernando da Costa
dc.subject.por.fl_str_mv Flavivírus
Vírus Rocio
Infecção experimental
topic Flavivírus
Vírus Rocio
Infecção experimental
description Rocio virus (ROCV) is an encephalitic flavivirus endemic to Brazil. Experimental flavivirus infections have previously demonstrated a persistent infection and, in this study, we investigated the persistence of ROCV infection in golden hamsters (Mesocricetus auratus). The hamsters were infected intraperitoneally with 9.8 LD50/0.02 mL of ROCV and later anaesthetised and sacrificed at various time points over a 120-day period to collect of blood, urine and organ samples. The viral titres were quantified by real-time-polymerase chain reaction (qRT-PCR). The specimens were used to infect Vero cells and ROCV antigens in the cells were detected by immunefluorescence assay. The levels of antibodies were determined by the haemagglutination inhibition technique. A histopathological examination was performed on the tissues by staining with haematoxylin-eosin and detecting viral antigens by immunohistochemistry (IHC). ROCV induced a strong immune response and was pathogenic in hamsters through neuroinvasion. ROCV was recovered from Vero cells exposed to samples from the viscera, brain, blood, serum and urine and was detected by qRT-PCR in the brain, liver and blood for three months after infection. ROCV induced histopathological changes and the expression of viral antigens, which were detected by IHC in the liver, kidney, lung and brain up to four months after infection. These findings show that ROCV is pathogenic to golden hamsters and has the capacity to cause persistent infection in animals after intraperitoneal infection.
publishDate 2012
dc.date.issued.fl_str_mv 2012-08
dc.date.accessioned.fl_str_mv 2013-08-13T13:28:42Z
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dc.identifier.citation.fl_str_mv HENRIQUES, Daniele Freitas, et al. Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 5, p. 630-636, ago. 2012. Disponível em: <http://www.scielo.br/pdf/mioc/v107n5/09.pdf>. Acesso em: 17 jul. 2013. <http://dx.doi.org/10.1590/S0074-02762012000500009>.
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identifier_str_mv HENRIQUES, Daniele Freitas, et al. Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 5, p. 630-636, ago. 2012. Disponível em: <http://www.scielo.br/pdf/mioc/v107n5/09.pdf>. Acesso em: 17 jul. 2013. <http://dx.doi.org/10.1590/S0074-02762012000500009>.
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