The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio2.unb.br/jspui/handle/10482/48493 https://doi.org/10.3390/pharmaceutics15071864 https://orcid.org/0000-0003-1961-7281 https://orcid.org/0000-0002-1096-3236 https://orcid.org/0000-0003-1821-1992 |
Resumo: | Amphibian secretions have been extensively investigated for the production of bioactive molecules. Salamandrin-I is an antioxidant peptide, isolated from the skin secretion of the fire salamander, that has induced no toxicity in microglia or erythrocytes. Importantly, the administration of antioxidants may constitute an adequate therapeutic approach to cancer treatment. Here, with the purpose of better characterizing the therapeutic potential of salamandrin-I, we investigated whether this antioxidant peptide also exerts anticancer activity, using the human leukemia cell line HL-60 as a cancer model. Salamandrin-I treatment induced a significant reduction in HL-60 proliferation, which was accompanied by cell cycle arrest. Furthermore, the peptide-induced cell death showed a significant increase in the LDH release in HL-60 cells. The cellular toxicity exerted by salamandrin-I is possibly related to pyroptosis, since the HL-60 cells showed loss of mitochondrial membrane potential and hyperexpression of inflammasome components following the peptide treatment. This is the first demonstration of the anticancer potential of the salamandrin-I peptide. Such results are important, as they offer relevant insights into the field of cancer therapy and allow the design of future bioactive molecules using salamandrin-I as a template. |
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The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60PeptídeosAntioxidantesSalamandraLeucemiaPiroptoseAnfíbioAmphibian secretions have been extensively investigated for the production of bioactive molecules. Salamandrin-I is an antioxidant peptide, isolated from the skin secretion of the fire salamander, that has induced no toxicity in microglia or erythrocytes. Importantly, the administration of antioxidants may constitute an adequate therapeutic approach to cancer treatment. Here, with the purpose of better characterizing the therapeutic potential of salamandrin-I, we investigated whether this antioxidant peptide also exerts anticancer activity, using the human leukemia cell line HL-60 as a cancer model. Salamandrin-I treatment induced a significant reduction in HL-60 proliferation, which was accompanied by cell cycle arrest. Furthermore, the peptide-induced cell death showed a significant increase in the LDH release in HL-60 cells. The cellular toxicity exerted by salamandrin-I is possibly related to pyroptosis, since the HL-60 cells showed loss of mitochondrial membrane potential and hyperexpression of inflammasome components following the peptide treatment. This is the first demonstration of the anticancer potential of the salamandrin-I peptide. Such results are important, as they offer relevant insights into the field of cancer therapy and allow the design of future bioactive molecules using salamandrin-I as a template.Faculdade de Ciências da Saúde (FS)Departamento de Farmácia (FS FAR)Faculdade de Medicina (FM)Instituto de Química (IQ)Programa de Pós-Graduação em Patologia MolecularMDPIUniversity of Brasilia, Faculty of Health Sciences, Laboratory of Hematology and Stem Cells (LHCT)University of Brasilia, Faculty of Health Sciences, Laboratory of Hematology and Stem Cells (LHCT)University of Brasilia, Faculty of Health Sciences, Laboratory of Hematology and Stem Cells (LHCT)University of Brasilia, Faculty of Medicine, Research Center in Morphology and Applied Immunology, NuPMIAUniversity of Brasilia, Institute of ChemistryUniversity of Brasilia, Faculty of Medicine, Research Center in Morphology and Applied Immunology, NuPMIAUniversity of Porto, Faculty of Sciences, Department of Chemistry and Biochemistry, LAQV/REQUIMTEUniversity of Lincoln, School of Chemistry, The BridgeUniversity of Brasilia, Faculty of Health Sciences, Laboratory of Hematology and Stem Cells (LHCT)Carvalho, Amandda Évelin Silva deOliveira, Nakaly Natiely deMachado, Julia Viana LafetáMoreira, Daniel CarneiroBrand, Guilherme DottoLeite, José Roberto de Souza de AlmeidaPlácido, AlexandraEaton, PeterAraújo, Felipe Saldanha de2024-07-02T11:06:04Z2024-07-02T11:06:04Z2023-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCARVALHO, Amandda Évelin Silva et al. The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60. Pharmaceutics, [S. l.], 15, n. 7, 1864, 2023. DOI: https://doi.org/10.3390/pharmaceutics15071864. Disponível em: https://www.mdpi.com/1999-4923/15/7/1864. Acesso em: 02 jul. 2024.http://repositorio2.unb.br/jspui/handle/10482/48493https://doi.org/10.3390/pharmaceutics15071864https://orcid.org/0000-0003-1961-7281https://orcid.org/0000-0002-1096-3236https://orcid.org/0000-0003-1821-1992eng© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-07-02T11:06:04Zoai:repositorio.unb.br:10482/48493Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-07-02T11:06:04Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.none.fl_str_mv |
The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60 |
title |
The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60 |
spellingShingle |
The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60 Carvalho, Amandda Évelin Silva de Peptídeos Antioxidantes Salamandra Leucemia Piroptose Anfíbio |
title_short |
The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60 |
title_full |
The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60 |
title_fullStr |
The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60 |
title_full_unstemmed |
The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60 |
title_sort |
The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60 |
author |
Carvalho, Amandda Évelin Silva de |
author_facet |
Carvalho, Amandda Évelin Silva de Oliveira, Nakaly Natiely de Machado, Julia Viana Lafetá Moreira, Daniel Carneiro Brand, Guilherme Dotto Leite, José Roberto de Souza de Almeida Plácido, Alexandra Eaton, Peter Araújo, Felipe Saldanha de |
author_role |
author |
author2 |
Oliveira, Nakaly Natiely de Machado, Julia Viana Lafetá Moreira, Daniel Carneiro Brand, Guilherme Dotto Leite, José Roberto de Souza de Almeida Plácido, Alexandra Eaton, Peter Araújo, Felipe Saldanha de |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Brasilia, Faculty of Health Sciences, Laboratory of Hematology and Stem Cells (LHCT) University of Brasilia, Faculty of Health Sciences, Laboratory of Hematology and Stem Cells (LHCT) University of Brasilia, Faculty of Health Sciences, Laboratory of Hematology and Stem Cells (LHCT) University of Brasilia, Faculty of Medicine, Research Center in Morphology and Applied Immunology, NuPMIA University of Brasilia, Institute of Chemistry University of Brasilia, Faculty of Medicine, Research Center in Morphology and Applied Immunology, NuPMIA University of Porto, Faculty of Sciences, Department of Chemistry and Biochemistry, LAQV/REQUIMTE University of Lincoln, School of Chemistry, The Bridge University of Brasilia, Faculty of Health Sciences, Laboratory of Hematology and Stem Cells (LHCT) |
dc.contributor.author.fl_str_mv |
Carvalho, Amandda Évelin Silva de Oliveira, Nakaly Natiely de Machado, Julia Viana Lafetá Moreira, Daniel Carneiro Brand, Guilherme Dotto Leite, José Roberto de Souza de Almeida Plácido, Alexandra Eaton, Peter Araújo, Felipe Saldanha de |
dc.subject.por.fl_str_mv |
Peptídeos Antioxidantes Salamandra Leucemia Piroptose Anfíbio |
topic |
Peptídeos Antioxidantes Salamandra Leucemia Piroptose Anfíbio |
description |
Amphibian secretions have been extensively investigated for the production of bioactive molecules. Salamandrin-I is an antioxidant peptide, isolated from the skin secretion of the fire salamander, that has induced no toxicity in microglia or erythrocytes. Importantly, the administration of antioxidants may constitute an adequate therapeutic approach to cancer treatment. Here, with the purpose of better characterizing the therapeutic potential of salamandrin-I, we investigated whether this antioxidant peptide also exerts anticancer activity, using the human leukemia cell line HL-60 as a cancer model. Salamandrin-I treatment induced a significant reduction in HL-60 proliferation, which was accompanied by cell cycle arrest. Furthermore, the peptide-induced cell death showed a significant increase in the LDH release in HL-60 cells. The cellular toxicity exerted by salamandrin-I is possibly related to pyroptosis, since the HL-60 cells showed loss of mitochondrial membrane potential and hyperexpression of inflammasome components following the peptide treatment. This is the first demonstration of the anticancer potential of the salamandrin-I peptide. Such results are important, as they offer relevant insights into the field of cancer therapy and allow the design of future bioactive molecules using salamandrin-I as a template. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-01 2024-07-02T11:06:04Z 2024-07-02T11:06:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CARVALHO, Amandda Évelin Silva et al. The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60. Pharmaceutics, [S. l.], 15, n. 7, 1864, 2023. DOI: https://doi.org/10.3390/pharmaceutics15071864. Disponível em: https://www.mdpi.com/1999-4923/15/7/1864. Acesso em: 02 jul. 2024. http://repositorio2.unb.br/jspui/handle/10482/48493 https://doi.org/10.3390/pharmaceutics15071864 https://orcid.org/0000-0003-1961-7281 https://orcid.org/0000-0002-1096-3236 https://orcid.org/0000-0003-1821-1992 |
identifier_str_mv |
CARVALHO, Amandda Évelin Silva et al. The peptide salamandrin-I modulates components involved in Pyroptosis and induces cell death in human leukemia cell line HL-60. Pharmaceutics, [S. l.], 15, n. 7, 1864, 2023. DOI: https://doi.org/10.3390/pharmaceutics15071864. Disponível em: https://www.mdpi.com/1999-4923/15/7/1864. Acesso em: 02 jul. 2024. |
url |
http://repositorio2.unb.br/jspui/handle/10482/48493 https://doi.org/10.3390/pharmaceutics15071864 https://orcid.org/0000-0003-1961-7281 https://orcid.org/0000-0002-1096-3236 https://orcid.org/0000-0003-1821-1992 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
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Repositório Institucional da UnB |
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Repositório Institucional da UnB - Universidade de Brasília (UnB) |
repository.mail.fl_str_mv |
repositorio@unb.br |
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