Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation

Detalhes bibliográficos
Autor(a) principal: QUEIROZ, Sabrina Ribeiro de Almeida
Data de Publicação: 2018
Outros Autores: SILVA JÚNIOR, José Valter Joaquim, SILVA, Andrea Nazare Monteiro Rangel da, OLIVEIRA, Amanda Gomes de, SANTOS, Jefferson José da Silva, GIL, Laura Helena Vega Gonzales
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFPA
Texto Completo: http://repositorio.ufpa.br/jspui/handle/2011/10032
http://dx.doi.org/10.1590/0037-8682-0220-2017
Resumo: Introduction: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. Methods: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs. Results: The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. Conclusions: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application.
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spelling 2018-06-14T21:02:08Z2018-06-14T21:02:08Z2018-02QUEIROZ, Sabrina Ribeiro de Almeida et al. Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 1, p. 66-70, jan./fev. 2018. Disponível em: <http://repositorio.ufpa.br/jspui/handle/2011/10032>. Acesso em:.0037-86821678-9849http://repositorio.ufpa.br/jspui/handle/2011/10032http://dx.doi.org/10.1590/0037-8682-0220-2017Introduction: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. Methods: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs. Results: The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. Conclusions: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application.engUniversidade Federal do ParáUFPABrasilRevista da Sociedade Brasileira de Medicina Tropicalhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000100066&lng=pt&nrm=isoreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPACNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIAVírus da febre amarelaInfecção viralPartículas pseudo-infecciosasFlavivírusDevelopment and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article51016670http://lattes.cnpq.br/2423996203006578http://lattes.cnpq.br/2723386233005530http://lattes.cnpq.br/6715094307334101http://lattes.cnpq.br/2869167717462114http://lattes.cnpq.br/7463258251915213http://lattes.cnpq.br/7238518046265531QUEIROZ, Sabrina Ribeiro de AlmeidaSILVA JÚNIOR, José Valter JoaquimSILVA, Andrea Nazare Monteiro Rangel daOLIVEIRA, Amanda Gomes deSANTOS, Jefferson José da SilvaGIL, Laura Helena Vega Gonzalesinfo:eu-repo/semantics/openAccessORIGINALArtigo_DevelopmentCharacterizationPackaging.pdfArtigo_DevelopmentCharacterizationPackaging.pdfapplication/pdf1218835http://repositorio.ufpa.br/oai/bitstream/2011/10032/1/Artigo_DevelopmentCharacterizationPackaging.pdfa596b87089326587e79461cce324932bMD51CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.pt_BR.fl_str_mv Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
title Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
spellingShingle Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
QUEIROZ, Sabrina Ribeiro de Almeida
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA
Vírus da febre amarela
Infecção viral
Partículas pseudo-infecciosas
Flavivírus
title_short Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
title_full Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
title_fullStr Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
title_full_unstemmed Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
title_sort Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
author QUEIROZ, Sabrina Ribeiro de Almeida
author_facet QUEIROZ, Sabrina Ribeiro de Almeida
SILVA JÚNIOR, José Valter Joaquim
SILVA, Andrea Nazare Monteiro Rangel da
OLIVEIRA, Amanda Gomes de
SANTOS, Jefferson José da Silva
GIL, Laura Helena Vega Gonzales
author_role author
author2 SILVA JÚNIOR, José Valter Joaquim
SILVA, Andrea Nazare Monteiro Rangel da
OLIVEIRA, Amanda Gomes de
SANTOS, Jefferson José da Silva
GIL, Laura Helena Vega Gonzales
author2_role author
author
author
author
author
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2423996203006578
http://lattes.cnpq.br/2723386233005530
http://lattes.cnpq.br/6715094307334101
http://lattes.cnpq.br/2869167717462114
http://lattes.cnpq.br/7463258251915213
http://lattes.cnpq.br/7238518046265531
dc.contributor.author.fl_str_mv QUEIROZ, Sabrina Ribeiro de Almeida
SILVA JÚNIOR, José Valter Joaquim
SILVA, Andrea Nazare Monteiro Rangel da
OLIVEIRA, Amanda Gomes de
SANTOS, Jefferson José da Silva
GIL, Laura Helena Vega Gonzales
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA
topic CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA
Vírus da febre amarela
Infecção viral
Partículas pseudo-infecciosas
Flavivírus
dc.subject.por.fl_str_mv Vírus da febre amarela
Infecção viral
Partículas pseudo-infecciosas
Flavivírus
description Introduction: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. Methods: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs. Results: The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. Conclusions: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-06-14T21:02:08Z
dc.date.available.fl_str_mv 2018-06-14T21:02:08Z
dc.date.issued.fl_str_mv 2018-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv QUEIROZ, Sabrina Ribeiro de Almeida et al. Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 1, p. 66-70, jan./fev. 2018. Disponível em: <http://repositorio.ufpa.br/jspui/handle/2011/10032>. Acesso em:.
dc.identifier.uri.fl_str_mv http://repositorio.ufpa.br/jspui/handle/2011/10032
dc.identifier.issn.pt_BR.fl_str_mv 0037-8682
1678-9849
dc.identifier.doi.pt_BR.fl_str_mv http://dx.doi.org/10.1590/0037-8682-0220-2017
identifier_str_mv QUEIROZ, Sabrina Ribeiro de Almeida et al. Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 1, p. 66-70, jan./fev. 2018. Disponível em: <http://repositorio.ufpa.br/jspui/handle/2011/10032>. Acesso em:.
0037-8682
1678-9849
url http://repositorio.ufpa.br/jspui/handle/2011/10032
http://dx.doi.org/10.1590/0037-8682-0220-2017
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language eng
dc.relation.ispartof.pt_BR.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Universidade Federal do Pará
dc.publisher.initials.fl_str_mv UFPA
dc.publisher.country.fl_str_mv Brasil
publisher.none.fl_str_mv Universidade Federal do Pará
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFPA
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