Modulação da expressão gênica caruncular pelo embrião bovino durante a placentação: Influência no metabolismo e transporte celularr

• Autor(a) principal: Silva, Kaetillyn Araújo Diniz da
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
• Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/15386
• topic: Inseminação artificial; Fertilização in vitro; Perdas gestacionais; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA

Despite the intense evolution reproductive biotech pregnancy failures still can not be avoided. The beginning of pregnancy is a critical period because it is associated with a higher number of failures in the development of pregnancy. In humans, this number varies between 15% and 19% spontaneus pregnancies. Failures in the implantation are still higher after IVF, reaching losses of 80-90% of human embryos. In cattle, when considering pregnancies of in vitro produced embryos such losses are even more significant. Manipulated embryos are more prone to failure. In order to determine the influence of bovine embryo on gene expression of SLC2A1, AQP1, AQP11 and GATM in the placenta were collected cotyledon-associated caruncles and caruncles from non-gravid horn were collected of cows submitted to artificial insemination (AI) and in vitro fertilization (IVF) at 30, 35 and 40 days of gestation. The relative expression of the genes was determined by real time qPCR. The genes were selected according to their activity in metabolism and cellular transport. AQP1 showed higher transcript at 30 and 35 days in IA pregnancies, but in IVF pregnancies, the term was reduced to 35 days in cotyledon-associated caruncles horn. AQP11 expression was increased in cotyledon-associated caruncles horn throughout the analyzed period in AI and IVF pregnancies and SLC2A1 showed higher expression in cotyledon-associated caruncles in IA pregnancies, however, there were no significant differences in IVF pregnancies. GATM showed lower expression in cotyledon-associated caruncles throughout the analyzed period in IA pregnancies, but this lower expression was observed only after 40 days in IVF pregnancies. The results indicate that the embryo modulates the dynamics of uterine gene expression during placentation and the embryo manipulation can alter the gene expression profile in this period.