Ocorrência de enterobactérias produtoras de beta lactamase de espectro ampliado (Esbl) em carcaças de frango comercializadas no estado da Paraíba

• Autor(a) principal: Vasconcelos, Priscylla Carvalho
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
• Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/15861
• topic: Carcaças de frangos – Resistência aos antimicrobianos; Carnes de frango – Resistência bacteriana; Escherichia spp; Klebsiella spp; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA

Antimicrobial resistance has been increasingly observed in bacteria isolated from both humans and animals. The production of beta lactamases is very common and is considered the main mechanism of resistance against beta-lactam antimicrobials in Gram-negative bacteria. However, the occurrence of extended-spectrum beta-lactamases producing bacteria is of major concern, since they are also resistant against non-lactam drugs, such as fluoroquinolones e aminoglycosides could limit therapeutic options in humans. In this sense, the monitoring of ESBL-producing bacteria in the food chain is important to ultimately decrease infections in humans given the fact that resistant genes can be transferred to pathogenic bacteria. The aim of this study was to investigate the occurrence of extended spectrum beta lactamases (ESBL) in enterobacteria cultured from chicken carcasses marketed in Paraiba State, Brazil. A total of 50 carcasses (fresh, frozen, or cooled) were rinsed using buffered peptone water. After incubation, aliquots (20 μL) were transferred to Brain Heart Infusion broth supplemented with ceftriaxone (1 mg/L). Culture was performed using MacConkey agar plates and isolated bacteria identified by means of phenotypic tests. Antimicrobial susceptibility test was performed using disk diffusion method (Kirby-Bauer). Furthermore, Double Disc Synergy Tests (DDST) was performed to identify ESBL and carbapenemases. A total of 150 isolates were cultured and Escherichia spp. (35.1%) Klebsiella spp. (33.1%) and Pseudomonas spp. (21.2%) were the most prevalent genera. Among confirmed ESBL-producing bacteria, 15 (30%) Klebsiella spp., 7 (13.20%) E. coli, 1 (3,2%) Pseudomonas spp. and 1 Shigella spp. were identified. Although cefoxitin and ceftriaxone were the most sensitive drugs to confirm ESBL by the DDST test, a positive association between those drugs was observed. Pseudomonas isolates showing antimicrobial resistance against carbapenems, mainly ertapenem (17%) and meropenem (3,5%) is of great concern and reinforce the need to further investigate the occurrence of antimicrobial resistance in bacteria from chicken carcasses marketed in Paraiba State and their real impact in public health.