Análise da toxicidade reprodutiva do extrato da semente de azadirachta indica em ratos wistar machos

Detalhes bibliográficos
Autor(a) principal: Silva, Hugo Thyares Fonseca Nascimento Pereira da
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/29749
Resumo: Azadirachta indica A. Juss, popularly known as NEEM, is a tree species native to India belonging to the Meliaceae family. Currently its leaves, bark and seeds have been used in the manufacture of herbicides, repellents, drugs (of therapeutic function), cosmetics, among others. The present study aimed to evaluate the reproductive toxicity of Neem seed extract in male Wistar rats after acute exposure at a dose of 2,000 mg/kg and the possible morphophysiological changes caused in spermatogenesis, through sperm analysis, hormonal biochemistry, histopathology and morphometrics of rats treated, seeking to verify the viability of its use as raw material in the development of oral contraceptives for animals. Sixteen male Wistar Rattus norvegicus, with an average age of 30 weeks, weighing an average of 375g ± 4.3428g were used. The animals were divided into two groups: control group and treated group. Each group was formed by 8 males. Morphometric analysis of testicular epithelium sections showed a significant reduction in epithelium height and tubular diameter in the treated group. The histopathology of the testicles of the treated rats showed irregular contours, characterized by a tunica albuginea with variations in the thickness of the dense connective tissue, moderate indentations in the testicular parenchyma, in addition to capsular detachment. In the tubular space, varying degrees of epithelial degeneration and necrosis were observed. The seminiferous tubules exhibited clusters in the tubular lumen, and a reduction in motility, vigor and concentration of cells in the epididymis was also observed. Regarding the morphology of the spermatozoa, one can find (with 100X magnification) changes in the shape of the head, with some of these cells presenting a pear-shaped head, an underdeveloped head and a strongly bent tail. Based on the analyses, it is concluded that the treatment with the aqueous extract of the seed of Azadirachta indica, orally (gavage) at a dose of 2000mg/kg caused negative alterations in the testis and epididymis of rats, verifying the reproductive toxicity of the extract of Neem, which can be used in the study and development of contraceptive medications for animal use.
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spelling Análise da toxicidade reprodutiva do extrato da semente de azadirachta indica em ratos wistar machosmorfometriaNeemtoxicidade reprodutivaCNPQ::CIENCIAS AGRARIASAzadirachta indica A. Juss, popularly known as NEEM, is a tree species native to India belonging to the Meliaceae family. Currently its leaves, bark and seeds have been used in the manufacture of herbicides, repellents, drugs (of therapeutic function), cosmetics, among others. The present study aimed to evaluate the reproductive toxicity of Neem seed extract in male Wistar rats after acute exposure at a dose of 2,000 mg/kg and the possible morphophysiological changes caused in spermatogenesis, through sperm analysis, hormonal biochemistry, histopathology and morphometrics of rats treated, seeking to verify the viability of its use as raw material in the development of oral contraceptives for animals. Sixteen male Wistar Rattus norvegicus, with an average age of 30 weeks, weighing an average of 375g ± 4.3428g were used. The animals were divided into two groups: control group and treated group. Each group was formed by 8 males. Morphometric analysis of testicular epithelium sections showed a significant reduction in epithelium height and tubular diameter in the treated group. The histopathology of the testicles of the treated rats showed irregular contours, characterized by a tunica albuginea with variations in the thickness of the dense connective tissue, moderate indentations in the testicular parenchyma, in addition to capsular detachment. In the tubular space, varying degrees of epithelial degeneration and necrosis were observed. The seminiferous tubules exhibited clusters in the tubular lumen, and a reduction in motility, vigor and concentration of cells in the epididymis was also observed. Regarding the morphology of the spermatozoa, one can find (with 100X magnification) changes in the shape of the head, with some of these cells presenting a pear-shaped head, an underdeveloped head and a strongly bent tail. Based on the analyses, it is concluded that the treatment with the aqueous extract of the seed of Azadirachta indica, orally (gavage) at a dose of 2000mg/kg caused negative alterations in the testis and epididymis of rats, verifying the reproductive toxicity of the extract of Neem, which can be used in the study and development of contraceptive medications for animal use.A Azadirachta indica A. Juss, popularmente conhecida como NEEM, é uma espécie arbórea nativa da Índia pertencente à família Meliaceae. Atualmente suas folhas, cascas e sementes, vêm sendo utilizadas na fabricação de herbicidas, repelentes, fármacos, cosméticos, entre outros. O presente estudo objetivou avaliar a toxicidade reprodutiva do extrato da semente de Neem em ratos Wistar machos após exposição aguda na dose de 2.000 mg/kg e as possíveis alterações morfofisiológicas causadas na espermatogênese, através de análise espermática, bioquímica hormonal, histopatológica e morfométrica dos ratos tratados, buscando verificar a viabilidade de sua utilização como matéria prima no desenvolvimento de contraceptivo oral. Foram utilizados 16 Rattus norvegicus, linhagem Wistar machos, com idade média de 30 semanas, pesando uma média de 375g ± 4.3428g. Os animais foram divididos em dois grupos: grupo controle e grupo tratado. Cada grupo foi formado por 8 machos. A análise morfométrica dos cortes de epitélio testicular exibiu redução significativa na altura do epitélio e no diâmetro tubular do grupo tratado. O histopatológico dos testículos dos ratos tratados demonstrou contornos irregulares, caracterizados por uma túnica albugínea com variações na espessura do tecido conjuntivo denso, moderadas reentrâncias no parênquima testicular, além de descolamento capsular. No espaço tubular, observou-se graus variados de degeneração e necrose epitelial. Os túbulos seminíferos exibiam aglomerados no lúmen tubular, constatando-se ainda redução nos parâmetros motilidade, vigor e concentração das células no epidídimo. Com relação a morfologia dos espermatozoides, pode-se encontrar (com aumento de 100X) alterações no formato da cabeça, com algumas dessas células apresentando cabeça piriforme, cabeça subdesenvolvida e cauda fortemente dobrada. Com base nas análises, conclui-se que o tratamento com o extrato aquoso da semente de Azadirachta indica, por via oral (gavagem) na dose de 2000mg/kg ocasionou alterações negativas no testículo e epidídimo de ratos, constatando a toxicidade reprodutiva do extrato de Neem, podendo o mesmo ser utilizado no estudo e desenvolvimento de medicações contraceptivas para uso animal.Universidade Federal da ParaíbaBrasilCiências VeterináriasPrograma de Pós-Graduação em Ciência AnimalUFPBGuerra, Ricardo Romãohttp://lattes.cnpq.br/5292006097455086Rola, Luciana Dinizhttp://lattes.cnpq.br/8886538320419786Nascimento, Claudenice Rodrigues dohttp://lattes.cnpq.br/4618189190140909Silva, Hugo Thyares Fonseca Nascimento Pereira da2024-03-05T17:35:45Z2023-05-162024-03-05T17:35:45Z2022-12-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttps://repositorio.ufpb.br/jspui/handle/123456789/29749porinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2024-03-06T06:07:25Zoai:repositorio.ufpb.br:123456789/29749Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2024-03-06T06:07:25Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false
dc.title.none.fl_str_mv Análise da toxicidade reprodutiva do extrato da semente de azadirachta indica em ratos wistar machos
title Análise da toxicidade reprodutiva do extrato da semente de azadirachta indica em ratos wistar machos
spellingShingle Análise da toxicidade reprodutiva do extrato da semente de azadirachta indica em ratos wistar machos
Silva, Hugo Thyares Fonseca Nascimento Pereira da
morfometria
Neem
toxicidade reprodutiva
CNPQ::CIENCIAS AGRARIAS
title_short Análise da toxicidade reprodutiva do extrato da semente de azadirachta indica em ratos wistar machos
title_full Análise da toxicidade reprodutiva do extrato da semente de azadirachta indica em ratos wistar machos
title_fullStr Análise da toxicidade reprodutiva do extrato da semente de azadirachta indica em ratos wistar machos
title_full_unstemmed Análise da toxicidade reprodutiva do extrato da semente de azadirachta indica em ratos wistar machos
title_sort Análise da toxicidade reprodutiva do extrato da semente de azadirachta indica em ratos wistar machos
author Silva, Hugo Thyares Fonseca Nascimento Pereira da
author_facet Silva, Hugo Thyares Fonseca Nascimento Pereira da
author_role author
dc.contributor.none.fl_str_mv Guerra, Ricardo Romão
http://lattes.cnpq.br/5292006097455086
Rola, Luciana Diniz
http://lattes.cnpq.br/8886538320419786
Nascimento, Claudenice Rodrigues do
http://lattes.cnpq.br/4618189190140909
dc.contributor.author.fl_str_mv Silva, Hugo Thyares Fonseca Nascimento Pereira da
dc.subject.por.fl_str_mv morfometria
Neem
toxicidade reprodutiva
CNPQ::CIENCIAS AGRARIAS
topic morfometria
Neem
toxicidade reprodutiva
CNPQ::CIENCIAS AGRARIAS
description Azadirachta indica A. Juss, popularly known as NEEM, is a tree species native to India belonging to the Meliaceae family. Currently its leaves, bark and seeds have been used in the manufacture of herbicides, repellents, drugs (of therapeutic function), cosmetics, among others. The present study aimed to evaluate the reproductive toxicity of Neem seed extract in male Wistar rats after acute exposure at a dose of 2,000 mg/kg and the possible morphophysiological changes caused in spermatogenesis, through sperm analysis, hormonal biochemistry, histopathology and morphometrics of rats treated, seeking to verify the viability of its use as raw material in the development of oral contraceptives for animals. Sixteen male Wistar Rattus norvegicus, with an average age of 30 weeks, weighing an average of 375g ± 4.3428g were used. The animals were divided into two groups: control group and treated group. Each group was formed by 8 males. Morphometric analysis of testicular epithelium sections showed a significant reduction in epithelium height and tubular diameter in the treated group. The histopathology of the testicles of the treated rats showed irregular contours, characterized by a tunica albuginea with variations in the thickness of the dense connective tissue, moderate indentations in the testicular parenchyma, in addition to capsular detachment. In the tubular space, varying degrees of epithelial degeneration and necrosis were observed. The seminiferous tubules exhibited clusters in the tubular lumen, and a reduction in motility, vigor and concentration of cells in the epididymis was also observed. Regarding the morphology of the spermatozoa, one can find (with 100X magnification) changes in the shape of the head, with some of these cells presenting a pear-shaped head, an underdeveloped head and a strongly bent tail. Based on the analyses, it is concluded that the treatment with the aqueous extract of the seed of Azadirachta indica, orally (gavage) at a dose of 2000mg/kg caused negative alterations in the testis and epididymis of rats, verifying the reproductive toxicity of the extract of Neem, which can be used in the study and development of contraceptive medications for animal use.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-30
2023-05-16
2024-03-05T17:35:45Z
2024-03-05T17:35:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufpb.br/jspui/handle/123456789/29749
url https://repositorio.ufpb.br/jspui/handle/123456789/29749
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Ciências Veterinárias
Programa de Pós-Graduação em Ciência Animal
UFPB
publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Ciências Veterinárias
Programa de Pós-Graduação em Ciência Animal
UFPB
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFPB
instname:Universidade Federal da Paraíba (UFPB)
instacron:UFPB
instname_str Universidade Federal da Paraíba (UFPB)
instacron_str UFPB
institution UFPB
reponame_str Biblioteca Digital de Teses e Dissertações da UFPB
collection Biblioteca Digital de Teses e Dissertações da UFPB
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)
repository.mail.fl_str_mv diretoria@ufpb.br|| diretoria@ufpb.br
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