Caracterização de esponjas nanoestruturadas de quitosana e policaprolactona para hemostasia
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFPB |
Texto Completo: | https://repositorio.ufpb.br/jspui/handle/123456789/18961 |
Resumo: | Introduction: Regenerative medicine has analyzed biological effects of natural polymers, as an attempt to create materials which can be used for tissue repair purposes, such as hemostasis. Among these materials we can highlight the chitosan that has been studied because of its hemostatic property. Objective: This study aimed to characterize sponges made of chitosan (CS) and polycaprolactone (PCL) blends for possible use as a topical hemostatic agent. Material e Methods: Chitosan solutions were prepared in 1% acetic acid and PCL in acetone. These were associated in the following volumetric proportions (v/v): 100:0; 75:25; 50:50; 25:75; 0:100, then they were frozen at -80°C and lyophilized to form the sponges by a freeze-drying method. The samples were subjected to characterizations of Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy (FTIR). It was held also an assessment of the interaction between sponges and blood tissue through the hemagglutination assay. Results: It was possible to observe the formation of chitosan sponges (CS) and CS/PCL, while when the solution of the pure PCL was used, it was observed that there was no sponge formation. The FTIR spectra revealed that there were no significant changes in the characteristic frequency of the functional groups when analyzed CS/PCL sponges when compared to spectra of pure polymers. The positive aspect hemagglutination was observed in the wells containing the pure chitosan sponges and CS75/PCL25. The hemolysis values were CS: 12.7%; CS50/PCL50: 9.6%; CS75/PCL25: 23.2%; CS25/PCL75: 6.5% e PCL: 3.52%. Conclusion: The blends of CS/PCL developed in this study were able to form sponges which showed intermediate chemical-physical characteristics between the materials, as well as hemagglutinating capacity, thus constituting a feasible alternative topical hemostatic agent for biomedical use. |
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Caracterização de esponjas nanoestruturadas de quitosana e policaprolactona para hemostasiaHemostasiaQuitosanaPolicaprolactonaHemostasisChitosanPolycaprolactoneCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAIntroduction: Regenerative medicine has analyzed biological effects of natural polymers, as an attempt to create materials which can be used for tissue repair purposes, such as hemostasis. Among these materials we can highlight the chitosan that has been studied because of its hemostatic property. Objective: This study aimed to characterize sponges made of chitosan (CS) and polycaprolactone (PCL) blends for possible use as a topical hemostatic agent. Material e Methods: Chitosan solutions were prepared in 1% acetic acid and PCL in acetone. These were associated in the following volumetric proportions (v/v): 100:0; 75:25; 50:50; 25:75; 0:100, then they were frozen at -80°C and lyophilized to form the sponges by a freeze-drying method. The samples were subjected to characterizations of Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy (FTIR). It was held also an assessment of the interaction between sponges and blood tissue through the hemagglutination assay. Results: It was possible to observe the formation of chitosan sponges (CS) and CS/PCL, while when the solution of the pure PCL was used, it was observed that there was no sponge formation. The FTIR spectra revealed that there were no significant changes in the characteristic frequency of the functional groups when analyzed CS/PCL sponges when compared to spectra of pure polymers. The positive aspect hemagglutination was observed in the wells containing the pure chitosan sponges and CS75/PCL25. The hemolysis values were CS: 12.7%; CS50/PCL50: 9.6%; CS75/PCL25: 23.2%; CS25/PCL75: 6.5% e PCL: 3.52%. Conclusion: The blends of CS/PCL developed in this study were able to form sponges which showed intermediate chemical-physical characteristics between the materials, as well as hemagglutinating capacity, thus constituting a feasible alternative topical hemostatic agent for biomedical use.NenhumaIntrodução: A medicina regenerativa tem avaliado os efeitos biológicos de polímeros naturais, na tentativa de criar materiais que possam ser usados para fins de reparo tecidual, como a hemostasia. Dentre estes materiais podemos destacar a quitosana que tem sido estudado devido sua propriedade hemostática. Objetivo: O presente trabalho teve como objetivo caracterizar esponjas de blendas constituídas por quitosana (QS) e policaprolactona (PCL) para possível utilização como agente hemostático tópico. Material e Métodos: Foram preparadas soluções de quitosana em ácido acético a 1% e de PCL em acetona. Estas foram associadas nas seguintes proporções volumétricas (v/v): 100:0; 75:25; 50:50; 25:75; 0:100, e então congeladas a -80 °C e logo após liofilizadas para formar as esponjas, conforme a metodologia freeze-drying. As amostras foram submetidas a caracterizações de Microscopia Eletrônica de Varredura (MEV), análise de espectroscopia na região do infravermelho com transformada de Fourier (FTIR) e uma avaliação da interação das esponjas com tecido sanguíneo através do ensaio de hemaglutinação. Resultados: Foi possível observar a formação de esponjas de quitosana (QS) e QS/PCL, já quando foi utilizada a solução pura do PCL, observou-se que não houve a formação da esponja. Nos espectros de FTIR observou-se que não houve mudanças significativas da frequência característica dos grupos funcionais quando analisamos as esponjas de QS/PCL se comparados aos espectros dos polímeros puros. O aspecto de hemaglutinação positiva foi observado nos poços que continham as esponjas de quitosana pura e QS75/PCL25. Os valores de hemólise foram QS:12,7%; QS50/PCL50: 9,6%; QS75/PCL25: 23,2%; QS25/PCL75: 6,5% e PCL: 3,52%. Conclusão: As blendas de QS/PCL, desenvolvidas no presente estudo, foram capazes de formar esponjas, apresentando características físico-químicas intermediárias entre os materiais, bem como capacidade hemaglutinante, constituindo assim uma possível alternativa de agente hemostático tópico para utilização biomédica.Universidade Federal da ParaíbaBrasilOdontologiaPrograma de Pós-Graduação em OdontologiaUFPBOliveira, Juliano Elvis dehttp://lattes.cnpq.br/4990968421738051Batista, André Ulisses Dantashttp://lattes.cnpq.br/9510942902249475Fonseca, Alessandra Gabriela Leonel2020-12-28T05:51:42Z2019-04-012020-12-28T05:51:42Z2016-01-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttps://repositorio.ufpb.br/jspui/handle/123456789/18961porAttribution-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nd/3.0/br/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2021-08-26T13:30:18Zoai:repositorio.ufpb.br:123456789/18961Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2021-08-26T13:30:18Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false |
dc.title.none.fl_str_mv |
Caracterização de esponjas nanoestruturadas de quitosana e policaprolactona para hemostasia |
title |
Caracterização de esponjas nanoestruturadas de quitosana e policaprolactona para hemostasia |
spellingShingle |
Caracterização de esponjas nanoestruturadas de quitosana e policaprolactona para hemostasia Fonseca, Alessandra Gabriela Leonel Hemostasia Quitosana Policaprolactona Hemostasis Chitosan Polycaprolactone CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Caracterização de esponjas nanoestruturadas de quitosana e policaprolactona para hemostasia |
title_full |
Caracterização de esponjas nanoestruturadas de quitosana e policaprolactona para hemostasia |
title_fullStr |
Caracterização de esponjas nanoestruturadas de quitosana e policaprolactona para hemostasia |
title_full_unstemmed |
Caracterização de esponjas nanoestruturadas de quitosana e policaprolactona para hemostasia |
title_sort |
Caracterização de esponjas nanoestruturadas de quitosana e policaprolactona para hemostasia |
author |
Fonseca, Alessandra Gabriela Leonel |
author_facet |
Fonseca, Alessandra Gabriela Leonel |
author_role |
author |
dc.contributor.none.fl_str_mv |
Oliveira, Juliano Elvis de http://lattes.cnpq.br/4990968421738051 Batista, André Ulisses Dantas http://lattes.cnpq.br/9510942902249475 |
dc.contributor.author.fl_str_mv |
Fonseca, Alessandra Gabriela Leonel |
dc.subject.por.fl_str_mv |
Hemostasia Quitosana Policaprolactona Hemostasis Chitosan Polycaprolactone CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
topic |
Hemostasia Quitosana Policaprolactona Hemostasis Chitosan Polycaprolactone CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Introduction: Regenerative medicine has analyzed biological effects of natural polymers, as an attempt to create materials which can be used for tissue repair purposes, such as hemostasis. Among these materials we can highlight the chitosan that has been studied because of its hemostatic property. Objective: This study aimed to characterize sponges made of chitosan (CS) and polycaprolactone (PCL) blends for possible use as a topical hemostatic agent. Material e Methods: Chitosan solutions were prepared in 1% acetic acid and PCL in acetone. These were associated in the following volumetric proportions (v/v): 100:0; 75:25; 50:50; 25:75; 0:100, then they were frozen at -80°C and lyophilized to form the sponges by a freeze-drying method. The samples were subjected to characterizations of Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy (FTIR). It was held also an assessment of the interaction between sponges and blood tissue through the hemagglutination assay. Results: It was possible to observe the formation of chitosan sponges (CS) and CS/PCL, while when the solution of the pure PCL was used, it was observed that there was no sponge formation. The FTIR spectra revealed that there were no significant changes in the characteristic frequency of the functional groups when analyzed CS/PCL sponges when compared to spectra of pure polymers. The positive aspect hemagglutination was observed in the wells containing the pure chitosan sponges and CS75/PCL25. The hemolysis values were CS: 12.7%; CS50/PCL50: 9.6%; CS75/PCL25: 23.2%; CS25/PCL75: 6.5% e PCL: 3.52%. Conclusion: The blends of CS/PCL developed in this study were able to form sponges which showed intermediate chemical-physical characteristics between the materials, as well as hemagglutinating capacity, thus constituting a feasible alternative topical hemostatic agent for biomedical use. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-29 2019-04-01 2020-12-28T05:51:42Z 2020-12-28T05:51:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufpb.br/jspui/handle/123456789/18961 |
url |
https://repositorio.ufpb.br/jspui/handle/123456789/18961 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Odontologia Programa de Pós-Graduação em Odontologia UFPB |
publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Odontologia Programa de Pós-Graduação em Odontologia UFPB |
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reponame:Biblioteca Digital de Teses e Dissertações da UFPB instname:Universidade Federal da Paraíba (UFPB) instacron:UFPB |
instname_str |
Universidade Federal da Paraíba (UFPB) |
instacron_str |
UFPB |
institution |
UFPB |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFPB |
collection |
Biblioteca Digital de Teses e Dissertações da UFPB |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB) |
repository.mail.fl_str_mv |
diretoria@ufpb.br|| diretoria@ufpb.br |
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1801843022098333696 |