Expressão gênica de transportadores de nutrientes e interleucinas no intestino de pintainhos suplementados com treonina In ovo e inoculados com Salmonella enteritidis

• Autor(a) principal: Andrade, Maria de Fátima de Souza
• Data de Publicação: 2019
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
• Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/14243
• topic: Aminoácidos; Ceco; Frangos; Transporte de nutrientes; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL

The presente study assessed the effects of threonine supplementation in ovo on the intestinal genic expression of chicks inoculated with Salmonella Enteritidis at 2 days of age. Glucose, peptide and amino acid transporter data are shown in Chapter II and cecum interleukin expression (IL-1β, IL-8, IL-17 and IL-22) data are shown in Chapter III. Threonine in ovo increased the expression of Glut2 and ASCT1 at hacth and the expression of all studied glucose transporters at two days of age, Sglt1, Glut2, Pept1 and ASCT1. At 168 hours post inoculation (hpi), there was no interaction between the two factors (threonine x Salmonella) for para Sglt1 and Glut2; Thr in ovo increased the expression of both genes and Salmonella decreased Glut2. Both expressions of PepT1 and ASCT1 were increased due to Thr in ovo independent of Salmonella inoculation status. For inoculated birds, Pept1 and ASCT1 expression was lower in birds supplemented in ovo with Thr. There was no interaction between the factors Threonine and Salmonella at 24 hpi, but there was interaction for IL-1β, IL-8 and IL-22 at 168 hpi. Supplementation in ovo with Thr decreased the expression of IL-1β at 24 hpi and IL-8 at 168 hours post-inoculation. Lower IL-1β expression was observed in birds supplemented with Thr in ovo, and inoculated or not, at 168 hours post-inoculation. The relative expression of all interleukins increased in birds inoculated with Salmonella after 24 hours, and IL-17 increased at 168 hpi. Salmonella increased the expression of IL-8, IL-17 e IL-22 in the birds that had been supplemented with Thr in ovo. The results indicate that Threonine supplemented to embryos of broilers increased the post-hatch expression of glucose, peptide and amino acid transporters in the absence of Salmonella and increases the expression of genes related to the immune system in birds with or without Salmonella challenge.