Estresses abióticos em plantas transformadas e não transformadas de tomate Micro-Tom com diferentes expressões da sHSP22 mitocondrial: Efeito do alagamento e de ciclos de alta e baixa temperatura.

Detalhes bibliográficos
Autor(a) principal: Hüther, Cristina Moll
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFPel - Guaiaca
Texto Completo: http://repositorio.ufpel.edu.br/handle/ri/2048
Resumo: The abiotic stress often cause metabolic disorders which cause a decrease in plant growth. In response to stress, an increase in the synthesis of certain proteins called ―heat shock proteins‖ (HSPs), might occur. The tomato (Lycopersicon esculentum) cv. Micro-Tom is regarded as a model for experimental studies, and that is because it has features that make it appropriate, such as small size, short development time and easy modification. This study aimed to evaluate the stress effects by flooding and by high and low temperatures in both modified and not modified ‗Micro-Tom tomato plants which have different mitochondrial expressions (MT- sHSP22). The first experiment was conducted in growth chambers under controlled conditions. When the plants were 50 days, they were submitted to stress by flooding during 14 days, and after 7 days, half the plants were removed from the flooding. In the second trial, plants were grown as in the first experiment, and when they presented themselves in a complete vegetative stage, they were submitted to thermal stress for a period of 24 hours of 10 ºC or 37 °C, soon after that, the plants were transferred to the initial conditions for 24 hours. After this phase they were again subjected to a new cycle of stress and recovery. On both experiments were evaluated parameters of the kinetic emission of chlorophyll fluorescence and of gas exchange, taking into account that in the first was also evaluated the content of chlorophyll, and, at the end of the test, the leaf area and the dry mass of the upper area and root. The flooding interfered on the the chlorophyll content of all genotypes, and on the genotype with a high expression of MT-sHSP22, a decrease in the leaf area was not identified and neither was in the total dry mass. The kinetics analysis of the transient fluorescence of chlorophyll showed differential effects of stress by flooding in the different parts of the photosynthetic machinery, and showed different responses between genotypes. When plants were submitted to stress by low temperature, a reduction in the liquid CO2 rate was noticed immediately after the stress, but, after recovery, all genotypes returned close to the controls. For plants submitted to high temperatures of the wild genotype and with high expression of MT-sHSP22 increased the liquid assimilation rate in comparison to control. The chlorophyll fluorescence data indicated different effects on absorption and utilization of light energy between the two genotypes for two of the temperature stress. The interpretation of the JIP test parameters helped to identify that three genotypes exhibit different behavior, mainly on high-temperature stress, and the plants of high expression genotypes of HSP22 and not transformed showed an increase in the parameters related to the photosystem I activity. These results suggest that plants with high doses of mitochondrial sHSP22 may present mechanisms of tolerance against the applied stress.
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spelling 2014-08-20T13:59:14Z2011-09-142014-08-20T13:59:14Z2011-08-10HUTHER, Cristina Moll. Abiotic stress in plants transformed and untransformed Micro-Tom tomato with different expressions of mitochondrial sHSP22: effect of flooding and the high and low temperature cycles.. 2011. 94 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2011.http://repositorio.ufpel.edu.br/handle/ri/2048The abiotic stress often cause metabolic disorders which cause a decrease in plant growth. In response to stress, an increase in the synthesis of certain proteins called ―heat shock proteins‖ (HSPs), might occur. The tomato (Lycopersicon esculentum) cv. Micro-Tom is regarded as a model for experimental studies, and that is because it has features that make it appropriate, such as small size, short development time and easy modification. This study aimed to evaluate the stress effects by flooding and by high and low temperatures in both modified and not modified ‗Micro-Tom tomato plants which have different mitochondrial expressions (MT- sHSP22). The first experiment was conducted in growth chambers under controlled conditions. When the plants were 50 days, they were submitted to stress by flooding during 14 days, and after 7 days, half the plants were removed from the flooding. In the second trial, plants were grown as in the first experiment, and when they presented themselves in a complete vegetative stage, they were submitted to thermal stress for a period of 24 hours of 10 ºC or 37 °C, soon after that, the plants were transferred to the initial conditions for 24 hours. After this phase they were again subjected to a new cycle of stress and recovery. On both experiments were evaluated parameters of the kinetic emission of chlorophyll fluorescence and of gas exchange, taking into account that in the first was also evaluated the content of chlorophyll, and, at the end of the test, the leaf area and the dry mass of the upper area and root. The flooding interfered on the the chlorophyll content of all genotypes, and on the genotype with a high expression of MT-sHSP22, a decrease in the leaf area was not identified and neither was in the total dry mass. The kinetics analysis of the transient fluorescence of chlorophyll showed differential effects of stress by flooding in the different parts of the photosynthetic machinery, and showed different responses between genotypes. When plants were submitted to stress by low temperature, a reduction in the liquid CO2 rate was noticed immediately after the stress, but, after recovery, all genotypes returned close to the controls. For plants submitted to high temperatures of the wild genotype and with high expression of MT-sHSP22 increased the liquid assimilation rate in comparison to control. The chlorophyll fluorescence data indicated different effects on absorption and utilization of light energy between the two genotypes for two of the temperature stress. The interpretation of the JIP test parameters helped to identify that three genotypes exhibit different behavior, mainly on high-temperature stress, and the plants of high expression genotypes of HSP22 and not transformed showed an increase in the parameters related to the photosystem I activity. These results suggest that plants with high doses of mitochondrial sHSP22 may present mechanisms of tolerance against the applied stress.Os estresses abióticos geralmente causam disfunções metabólicas que provocam diminuição no crescimento das plantas. Em respostas a estresses pode ocorrer o aumento na síntese de algumas proteínas chamadas proteínas de choque térmico (heat shock proteins, HSPs). O tomate (Lycopersicon esculentum), cv. Micro-Tom é considerado como um modelo para estudos experimentais, pois ele possui características que o tornam adequado, tais como o tamanho pequeno, tempo de geração curto, e facilidade de transformação. Este trabalho teve como objetivo avaliar os efeitos dos estresses por alagamento e por altas e baixas temperatura em plantas transformadas e não transformadas de tomate ‗Micro-Tom com diferentes expressões da sHSP22 mitocondrial (MT-sHSP22). O primeiro experimento foi conduzido em câmaras de crescimento, sob condições controladas. Quando as plantas apresentavam 50 dias foram submetidas a estresse por alagamento por um período de 14 dias, sendo que após 7 dias metade das plantas foram retiradas do alagamento. No segundo ensaio, as plantas foram cultivadas como no primeiro experimento, e quando se apresentavam em estádio vegetativo pleno foram submetidas a estresse térmico por período de 24h a 10ºC ou 37ºC, a seguir as plantas foram transferidas para as condições iniciais por 24h, após este período foram novamente submetidas a um novo ciclo de estresse e recuperação. Em ambos os experimentos foram avaliados os parâmetros da cinética de emissão da fluorescência da clorofila e trocas gasosas, sendo que no primeiro avaliou-se também o índice de clorofila, e ao final do ensaio a área foliar e massa seca da parte aérea e raiz. O alagamento interferiu nos teores de clorofila de todos os genótipos, sendo que no genótipo com elevada expressão da MT-sHSP22, não foi observada redução da área foliar e em relação a massa seca total. A análise da cinética da fluorescência transiente da clorofila a mostrou efeitos diferenciais do estresse por alagamento nos diferentes locais da maquinaria fotossintética, e apresentou variação de resposta entre os genótipos. Quando as plantas foram submetidas ao estresse por baixa temperatura, observou-se redução na taxa assimilatória liquida de CO2 imediatamente após o estresse, mas depois da recuperação todos os genótipos retornaram próximos aos controles. Para as plantas submetidas a altas temperaturas do genótipo selvagem e do com elevada expressão da MT-sHSP22 aumentaram a taxa assimilatória líquida em relação ao controle. Os dados de fluorescência das clorofilas indicaram efeitos diferentes na absorção e aproveitamento da energia luminosa entre os genótipos para os dois estresses de temperatura. A interpretação dos parâmetros do Teste JIP possibilitou identificar que os três genótipos apresentam comportamento distinto principalmente sobre estresse de alta temperatura, sendo que as plantas dos genótipos com alta expressão de sHSP22 e não transformado apresentaram elevação nos parâmetros relacionados a atividade do fotossistema I. Estes resultados sugerem que as plantas com elevada expressão das sHSP22 mitocondrial podem apresentar mecanismos de tolerância frente as estresses aplicados.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em Fisiologia VegetalUFPelBRBiologiaProteínaHSPTeste JIPFotossínteseEstressesProteinHSPJIP-testPhotosynthesisStressCNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETALEstresses abióticos em plantas transformadas e não transformadas de tomate Micro-Tom com diferentes expressões da sHSP22 mitocondrial: Efeito do alagamento e de ciclos de alta e baixa temperatura.Abiotic stress in plants transformed and untransformed Micro-Tom tomato with different expressions of mitochondrial sHSP22: effect of flooding and the high and low temperature cycles.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/5164391381813344http://lattes.cnpq.br/9778741221243155Bacarin, Marcos AntônioHüther, Cristina Mollinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_cristina_moll_huther.pdfapplication/pdf1943888http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2048/1/dissertacao_cristina_moll_huther.pdf19ce036e997295b4526288308a80fe3cMD51open accessTEXTdissertacao_cristina_moll_huther.pdf.txtdissertacao_cristina_moll_huther.pdf.txtExtracted Texttext/plain180172http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2048/2/dissertacao_cristina_moll_huther.pdf.txtb47c2567451f78807a71c2dcb0132395MD52open accessTHUMBNAILdissertacao_cristina_moll_huther.pdf.jpgdissertacao_cristina_moll_huther.pdf.jpgGenerated Thumbnailimage/jpeg1383http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2048/3/dissertacao_cristina_moll_huther.pdf.jpg7919c3b31fb43914145114ada3b4623cMD53open access123456789/20482020-04-17 09:57:19.59open accessoai:guaiaca.ufpel.edu.br:123456789/2048Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2020-04-17T12:57:19Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false
dc.title.por.fl_str_mv Estresses abióticos em plantas transformadas e não transformadas de tomate Micro-Tom com diferentes expressões da sHSP22 mitocondrial: Efeito do alagamento e de ciclos de alta e baixa temperatura.
dc.title.alternative.eng.fl_str_mv Abiotic stress in plants transformed and untransformed Micro-Tom tomato with different expressions of mitochondrial sHSP22: effect of flooding and the high and low temperature cycles.
title Estresses abióticos em plantas transformadas e não transformadas de tomate Micro-Tom com diferentes expressões da sHSP22 mitocondrial: Efeito do alagamento e de ciclos de alta e baixa temperatura.
spellingShingle Estresses abióticos em plantas transformadas e não transformadas de tomate Micro-Tom com diferentes expressões da sHSP22 mitocondrial: Efeito do alagamento e de ciclos de alta e baixa temperatura.
Hüther, Cristina Moll
Proteína
HSP
Teste JIP
Fotossíntese
Estresses
Protein
HSP
JIP-test
Photosynthesis
Stress
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL
title_short Estresses abióticos em plantas transformadas e não transformadas de tomate Micro-Tom com diferentes expressões da sHSP22 mitocondrial: Efeito do alagamento e de ciclos de alta e baixa temperatura.
title_full Estresses abióticos em plantas transformadas e não transformadas de tomate Micro-Tom com diferentes expressões da sHSP22 mitocondrial: Efeito do alagamento e de ciclos de alta e baixa temperatura.
title_fullStr Estresses abióticos em plantas transformadas e não transformadas de tomate Micro-Tom com diferentes expressões da sHSP22 mitocondrial: Efeito do alagamento e de ciclos de alta e baixa temperatura.
title_full_unstemmed Estresses abióticos em plantas transformadas e não transformadas de tomate Micro-Tom com diferentes expressões da sHSP22 mitocondrial: Efeito do alagamento e de ciclos de alta e baixa temperatura.
title_sort Estresses abióticos em plantas transformadas e não transformadas de tomate Micro-Tom com diferentes expressões da sHSP22 mitocondrial: Efeito do alagamento e de ciclos de alta e baixa temperatura.
author Hüther, Cristina Moll
author_facet Hüther, Cristina Moll
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/5164391381813344
dc.contributor.advisorLattes.por.fl_str_mv http://lattes.cnpq.br/9778741221243155
dc.contributor.advisor1.fl_str_mv Bacarin, Marcos Antônio
dc.contributor.author.fl_str_mv Hüther, Cristina Moll
contributor_str_mv Bacarin, Marcos Antônio
dc.subject.por.fl_str_mv Proteína
HSP
Teste JIP
Fotossíntese
Estresses
topic Proteína
HSP
Teste JIP
Fotossíntese
Estresses
Protein
HSP
JIP-test
Photosynthesis
Stress
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL
dc.subject.eng.fl_str_mv Protein
HSP
JIP-test
Photosynthesis
Stress
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL
description The abiotic stress often cause metabolic disorders which cause a decrease in plant growth. In response to stress, an increase in the synthesis of certain proteins called ―heat shock proteins‖ (HSPs), might occur. The tomato (Lycopersicon esculentum) cv. Micro-Tom is regarded as a model for experimental studies, and that is because it has features that make it appropriate, such as small size, short development time and easy modification. This study aimed to evaluate the stress effects by flooding and by high and low temperatures in both modified and not modified ‗Micro-Tom tomato plants which have different mitochondrial expressions (MT- sHSP22). The first experiment was conducted in growth chambers under controlled conditions. When the plants were 50 days, they were submitted to stress by flooding during 14 days, and after 7 days, half the plants were removed from the flooding. In the second trial, plants were grown as in the first experiment, and when they presented themselves in a complete vegetative stage, they were submitted to thermal stress for a period of 24 hours of 10 ºC or 37 °C, soon after that, the plants were transferred to the initial conditions for 24 hours. After this phase they were again subjected to a new cycle of stress and recovery. On both experiments were evaluated parameters of the kinetic emission of chlorophyll fluorescence and of gas exchange, taking into account that in the first was also evaluated the content of chlorophyll, and, at the end of the test, the leaf area and the dry mass of the upper area and root. The flooding interfered on the the chlorophyll content of all genotypes, and on the genotype with a high expression of MT-sHSP22, a decrease in the leaf area was not identified and neither was in the total dry mass. The kinetics analysis of the transient fluorescence of chlorophyll showed differential effects of stress by flooding in the different parts of the photosynthetic machinery, and showed different responses between genotypes. When plants were submitted to stress by low temperature, a reduction in the liquid CO2 rate was noticed immediately after the stress, but, after recovery, all genotypes returned close to the controls. For plants submitted to high temperatures of the wild genotype and with high expression of MT-sHSP22 increased the liquid assimilation rate in comparison to control. The chlorophyll fluorescence data indicated different effects on absorption and utilization of light energy between the two genotypes for two of the temperature stress. The interpretation of the JIP test parameters helped to identify that three genotypes exhibit different behavior, mainly on high-temperature stress, and the plants of high expression genotypes of HSP22 and not transformed showed an increase in the parameters related to the photosystem I activity. These results suggest that plants with high doses of mitochondrial sHSP22 may present mechanisms of tolerance against the applied stress.
publishDate 2011
dc.date.available.fl_str_mv 2011-09-14
2014-08-20T13:59:14Z
dc.date.issued.fl_str_mv 2011-08-10
dc.date.accessioned.fl_str_mv 2014-08-20T13:59:14Z
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dc.identifier.citation.fl_str_mv HUTHER, Cristina Moll. Abiotic stress in plants transformed and untransformed Micro-Tom tomato with different expressions of mitochondrial sHSP22: effect of flooding and the high and low temperature cycles.. 2011. 94 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2011.
dc.identifier.uri.fl_str_mv http://repositorio.ufpel.edu.br/handle/ri/2048
identifier_str_mv HUTHER, Cristina Moll. Abiotic stress in plants transformed and untransformed Micro-Tom tomato with different expressions of mitochondrial sHSP22: effect of flooding and the high and low temperature cycles.. 2011. 94 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2011.
url http://repositorio.ufpel.edu.br/handle/ri/2048
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dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Fisiologia Vegetal
dc.publisher.initials.fl_str_mv UFPel
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Biologia
publisher.none.fl_str_mv Universidade Federal de Pelotas
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