Silagem de colostro bovino: propriedades e potencialidades de usos

Detalhes bibliográficos
Autor(a) principal: Saalfeld, Mara Helena
Data de Publicação: 2013
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFPel - Guaiaca
Texto Completo: http://guaiaca.ufpel.edu.br/handle/123456789/1212
Resumo: Colostrum is considered essential for newborns as a nutritional source and transfer for passive immunity. The production of colostrum above the calf intake capacity enables using the surplus as a milk replacer. One way of using it is through the anaerobic fermentation commonly called as colostrum silage. This food provides adequate weight gain to the animals; however, there is no data concerning the result of anaerobic fermentation on its elements. The aim of this study was to assess the physic-chemical, microbiological and immunological characteristics of the colostrum silage and its usage as nutritional supplement in animal feeding and its usage in the production of food for human usage. The centesimal assessment demonstrated that colostrum has nutrients in bigger amounts than the ones found in milk and that the colostrum silage kept the nutrients found in colostrum in natura, mainly the proteins (14,24%), dry matter (17,54%) and fat (5,55%), with the exception of lactose which, 30 days after fermentation was not detected anymore. It was noticed that the pH values decreased while there was an increase of lactic acid concentrations. Animals fed with colostrum silage had a weight gain at weaning of 0.7 above (p<0.05) the control animals (food with milk) which presented weight gains of 0.6 kg/day. The microbiological assessment showed that 21 days after fermentation the pathogenic bacteriae tested were not detected anymore, while there were still lactic acid bacteriae identified through sequencing the 16S rDNA as Lactobacillus (75%) and Enterococcus (25%). Identified as Lactobacillus (75%) (L. casei, L. brevis, L. rennanqily, L. crustorum) and Enterococcus (25%) (E. durans, E. faecium E. faecalis and E. mundyii). The immunoglobulins assessment in colostrum silage was carried out through the Immuno enzymatic method (ELISA), in which it was proved that after 12 months of storage, the colostrums silage kept feasible immunoglobulins and these were transmitted to newborns in the same proportion as the colostrum in natura. It was possible to elaborate foods such as dairy beverage and butter from the colostrum silage keeping the nutritional characteristics and microbiota containing lactic acid bacteria. The carrying out of sensory analysis reached an acceptance of 85% and purchasing intention from the assessors. Thus, it can be concluded that colostrums silage is a food of high nutritional value, microbiologically innocuous food, with immunological capability, and that it can be used in animal feeding with the need of proper legislation to enable its usability as human food.
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spelling 2014-08-20T13:32:46Z2013-10-312014-08-20T13:32:46Z2013-05-22SAALFELD, Mara Helena. Silagem de colostro bovino: propriedades e potencialidades de usos. 2013. 98 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2013.http://guaiaca.ufpel.edu.br/handle/123456789/1212Colostrum is considered essential for newborns as a nutritional source and transfer for passive immunity. The production of colostrum above the calf intake capacity enables using the surplus as a milk replacer. One way of using it is through the anaerobic fermentation commonly called as colostrum silage. This food provides adequate weight gain to the animals; however, there is no data concerning the result of anaerobic fermentation on its elements. The aim of this study was to assess the physic-chemical, microbiological and immunological characteristics of the colostrum silage and its usage as nutritional supplement in animal feeding and its usage in the production of food for human usage. The centesimal assessment demonstrated that colostrum has nutrients in bigger amounts than the ones found in milk and that the colostrum silage kept the nutrients found in colostrum in natura, mainly the proteins (14,24%), dry matter (17,54%) and fat (5,55%), with the exception of lactose which, 30 days after fermentation was not detected anymore. It was noticed that the pH values decreased while there was an increase of lactic acid concentrations. Animals fed with colostrum silage had a weight gain at weaning of 0.7 above (p<0.05) the control animals (food with milk) which presented weight gains of 0.6 kg/day. The microbiological assessment showed that 21 days after fermentation the pathogenic bacteriae tested were not detected anymore, while there were still lactic acid bacteriae identified through sequencing the 16S rDNA as Lactobacillus (75%) and Enterococcus (25%). Identified as Lactobacillus (75%) (L. casei, L. brevis, L. rennanqily, L. crustorum) and Enterococcus (25%) (E. durans, E. faecium E. faecalis and E. mundyii). The immunoglobulins assessment in colostrum silage was carried out through the Immuno enzymatic method (ELISA), in which it was proved that after 12 months of storage, the colostrums silage kept feasible immunoglobulins and these were transmitted to newborns in the same proportion as the colostrum in natura. It was possible to elaborate foods such as dairy beverage and butter from the colostrum silage keeping the nutritional characteristics and microbiota containing lactic acid bacteria. The carrying out of sensory analysis reached an acceptance of 85% and purchasing intention from the assessors. Thus, it can be concluded that colostrums silage is a food of high nutritional value, microbiologically innocuous food, with immunological capability, and that it can be used in animal feeding with the need of proper legislation to enable its usability as human food.O colostro é considerado essencial para bovinos recém-nascidos como fonte nutricional e transferência de imunidade passiva. A produção de colostro acima da capacidade de ingestão da bezerra permite utilizar o excedente como sucedâneo lácteo. Uma das formas de utilização é através da fermentação anaeróbica popularmente chamada de silagem de colostro. Este alimento garante ao animal ganho de peso adequado, entretanto, não existem dados sobre o resultado da fermentação anaeróbica sobre seus constituintes. Objetivou-se neste estudo avaliar as características físico-químicas, microbiológicas e imunológicas da silagem de colostro e a sua utilização como suplemento nutricional em alimentação animal e o seu uso no desenvolvimento de produtos para consumo humano. A avaliação centesimal demonstrou que o colostro possui nutrientes em quantidades superiores ao leite e que a silagem de colostro manteve os nutrientes presentes no colostro in natura, principalmente proteínas (14,24%), matéria seca (17,54%) e gordura (5,55%), com exceção da lactose que, após 30 dias de fermentação, não foi mais detectada. Observou-se que os valores de pH diminuíram ao mesmo tempo que houve aumento das concentrações de ácido láctico. Animais alimentados com a silagem de colostro obtiveram ganho de peso ao desmame de 0,7Kg/dia, superiores (p<0.05) aos animais controle (alimentados com leite) que apresentaram ganhos de peso de 0,6kg/dia. A avaliação microbiológica demonstrou que após 21 dias de fermentação, as bactérias patogênicas testadas não foram mais detectadas, restando viáveis bactérias ácido lácticas que por sequenciamento da região 16S rDNA foram identificadas como Lactobacillus (75%) (L. casei, L. brevis, L. rennanqily, L. crustorum) e Enterococcus (25%) (E. durans, E. faecium, E. faecalis e E. mundyii). A avaliação das imunoglobulinas na silagem de colostro foi realizada por ELISA, ficando comprovado que após 12 meses de armazenamento a silagem de colostro manteve imunoglobulinas viáveis e estas foram transmitidas aos recém-nascidos na mesma proporção que o colostro in natura. Foi possível elaborar alimentos como, bebida láctea e manteiga, a partir da silagem de colostro, com manutenção das características nutricionais e microbiota constituída de bactérias ácido lácticas. A realização da análise sensorial obteve 85% de aprovação, com intenção de compra pelos avaliadores. Assim, pode-se concluir que a silagem de colostro é um alimento de elevado valor nutricional, microbiologicamente inócuo, com potencial imunológico, e que pode ser utilizado na alimentação animal após 21 dias de fermentação, necessitando adequações à legislação para o seu uso na alimentação humana.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em BiotecnologiaUFPelBRBiotecnologiaBiotechnologyColostrumCalvesImmunoglobulinsSilageNutritiousLactic acid BacteriaBiotecnologiaColostroBezerrosImunoglobulinasSilagemNutriçãoBactérias ácido láticasCNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMALSilagem de colostro bovino: propriedades e potencialidades de usosSilagem de colostro bovino: propriedades e potencialidades de usosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttp://lattes.cnpq.br/3053223522980282http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763076J5Pereira, Daniela Isabel Brayerhttp://lattes.cnpq.br/3382450720179401Gularte, Marcia Arochahttp://lattes.cnpq.br/5756521626319170Gandra, Eliezer Ávilahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4704972A2Leite, Fabio Pereira LeivasSaalfeld, Mara Helenainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALtese_Mara_Saalfeld.pdfapplication/pdf1507358http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1212/1/tese_Mara_Saalfeld.pdf268615e5bd86cf1b94f0f708eebbb5feMD51open accessTEXTtese_Mara_Saalfeld.pdf.txttese_Mara_Saalfeld.pdf.txtExtracted Texttext/plain154607http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1212/2/tese_Mara_Saalfeld.pdf.txtb51c3c97df30eb2ab69b71d02f0981c5MD52open accessTHUMBNAILtese_Mara_Saalfeld.pdf.jpgtese_Mara_Saalfeld.pdf.jpgGenerated Thumbnailimage/jpeg1928http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1212/3/tese_Mara_Saalfeld.pdf.jpg54fc1dcf6ca66ded75a762a802b90d0cMD53open access123456789/12122019-08-23 10:46:30.265open accessoai:guaiaca.ufpel.edu.br:123456789/1212Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-08-23T13:46:30Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false
dc.title.por.fl_str_mv Silagem de colostro bovino: propriedades e potencialidades de usos
dc.title.alternative.eng.fl_str_mv Silagem de colostro bovino: propriedades e potencialidades de usos
title Silagem de colostro bovino: propriedades e potencialidades de usos
spellingShingle Silagem de colostro bovino: propriedades e potencialidades de usos
Saalfeld, Mara Helena
Biotechnology
Colostrum
Calves
Immunoglobulins
Silage
Nutritious
Lactic acid Bacteria
Biotecnologia
Colostro
Bezerros
Imunoglobulinas
Silagem
Nutrição
Bactérias ácido láticas
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL
title_short Silagem de colostro bovino: propriedades e potencialidades de usos
title_full Silagem de colostro bovino: propriedades e potencialidades de usos
title_fullStr Silagem de colostro bovino: propriedades e potencialidades de usos
title_full_unstemmed Silagem de colostro bovino: propriedades e potencialidades de usos
title_sort Silagem de colostro bovino: propriedades e potencialidades de usos
author Saalfeld, Mara Helena
author_facet Saalfeld, Mara Helena
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/3053223522980282
dc.contributor.advisorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763076J5
dc.contributor.referees1.pt_BR.fl_str_mv Gandra, Eliezer Ávila
dc.contributor.referees1ID.por.fl_str_mv
dc.contributor.referees1Lattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4704972A2
dc.contributor.advisor-co1.fl_str_mv Pereira, Daniela Isabel Brayer
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/3382450720179401
dc.contributor.advisor-co2.fl_str_mv Gularte, Marcia Arocha
dc.contributor.advisor-co2Lattes.fl_str_mv http://lattes.cnpq.br/5756521626319170
dc.contributor.advisor1.fl_str_mv Leite, Fabio Pereira Leivas
dc.contributor.author.fl_str_mv Saalfeld, Mara Helena
contributor_str_mv Pereira, Daniela Isabel Brayer
Gularte, Marcia Arocha
Leite, Fabio Pereira Leivas
dc.subject.eng.fl_str_mv Biotechnology
Colostrum
Calves
Immunoglobulins
Silage
Nutritious
Lactic acid Bacteria
topic Biotechnology
Colostrum
Calves
Immunoglobulins
Silage
Nutritious
Lactic acid Bacteria
Biotecnologia
Colostro
Bezerros
Imunoglobulinas
Silagem
Nutrição
Bactérias ácido láticas
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL
dc.subject.por.fl_str_mv Biotecnologia
Colostro
Bezerros
Imunoglobulinas
Silagem
Nutrição
Bactérias ácido láticas
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL
description Colostrum is considered essential for newborns as a nutritional source and transfer for passive immunity. The production of colostrum above the calf intake capacity enables using the surplus as a milk replacer. One way of using it is through the anaerobic fermentation commonly called as colostrum silage. This food provides adequate weight gain to the animals; however, there is no data concerning the result of anaerobic fermentation on its elements. The aim of this study was to assess the physic-chemical, microbiological and immunological characteristics of the colostrum silage and its usage as nutritional supplement in animal feeding and its usage in the production of food for human usage. The centesimal assessment demonstrated that colostrum has nutrients in bigger amounts than the ones found in milk and that the colostrum silage kept the nutrients found in colostrum in natura, mainly the proteins (14,24%), dry matter (17,54%) and fat (5,55%), with the exception of lactose which, 30 days after fermentation was not detected anymore. It was noticed that the pH values decreased while there was an increase of lactic acid concentrations. Animals fed with colostrum silage had a weight gain at weaning of 0.7 above (p<0.05) the control animals (food with milk) which presented weight gains of 0.6 kg/day. The microbiological assessment showed that 21 days after fermentation the pathogenic bacteriae tested were not detected anymore, while there were still lactic acid bacteriae identified through sequencing the 16S rDNA as Lactobacillus (75%) and Enterococcus (25%). Identified as Lactobacillus (75%) (L. casei, L. brevis, L. rennanqily, L. crustorum) and Enterococcus (25%) (E. durans, E. faecium E. faecalis and E. mundyii). The immunoglobulins assessment in colostrum silage was carried out through the Immuno enzymatic method (ELISA), in which it was proved that after 12 months of storage, the colostrums silage kept feasible immunoglobulins and these were transmitted to newborns in the same proportion as the colostrum in natura. It was possible to elaborate foods such as dairy beverage and butter from the colostrum silage keeping the nutritional characteristics and microbiota containing lactic acid bacteria. The carrying out of sensory analysis reached an acceptance of 85% and purchasing intention from the assessors. Thus, it can be concluded that colostrums silage is a food of high nutritional value, microbiologically innocuous food, with immunological capability, and that it can be used in animal feeding with the need of proper legislation to enable its usability as human food.
publishDate 2013
dc.date.available.fl_str_mv 2013-10-31
2014-08-20T13:32:46Z
dc.date.issued.fl_str_mv 2013-05-22
dc.date.accessioned.fl_str_mv 2014-08-20T13:32:46Z
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dc.identifier.citation.fl_str_mv SAALFELD, Mara Helena. Silagem de colostro bovino: propriedades e potencialidades de usos. 2013. 98 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2013.
dc.identifier.uri.fl_str_mv http://guaiaca.ufpel.edu.br/handle/123456789/1212
identifier_str_mv SAALFELD, Mara Helena. Silagem de colostro bovino: propriedades e potencialidades de usos. 2013. 98 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2013.
url http://guaiaca.ufpel.edu.br/handle/123456789/1212
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dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Biotecnologia
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publisher.none.fl_str_mv Universidade Federal de Pelotas
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