Silagem de colostro bovino: propriedades e potencialidades de usos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://guaiaca.ufpel.edu.br/handle/123456789/1212 |
Resumo: | Colostrum is considered essential for newborns as a nutritional source and transfer for passive immunity. The production of colostrum above the calf intake capacity enables using the surplus as a milk replacer. One way of using it is through the anaerobic fermentation commonly called as colostrum silage. This food provides adequate weight gain to the animals; however, there is no data concerning the result of anaerobic fermentation on its elements. The aim of this study was to assess the physic-chemical, microbiological and immunological characteristics of the colostrum silage and its usage as nutritional supplement in animal feeding and its usage in the production of food for human usage. The centesimal assessment demonstrated that colostrum has nutrients in bigger amounts than the ones found in milk and that the colostrum silage kept the nutrients found in colostrum in natura, mainly the proteins (14,24%), dry matter (17,54%) and fat (5,55%), with the exception of lactose which, 30 days after fermentation was not detected anymore. It was noticed that the pH values decreased while there was an increase of lactic acid concentrations. Animals fed with colostrum silage had a weight gain at weaning of 0.7 above (p<0.05) the control animals (food with milk) which presented weight gains of 0.6 kg/day. The microbiological assessment showed that 21 days after fermentation the pathogenic bacteriae tested were not detected anymore, while there were still lactic acid bacteriae identified through sequencing the 16S rDNA as Lactobacillus (75%) and Enterococcus (25%). Identified as Lactobacillus (75%) (L. casei, L. brevis, L. rennanqily, L. crustorum) and Enterococcus (25%) (E. durans, E. faecium E. faecalis and E. mundyii). The immunoglobulins assessment in colostrum silage was carried out through the Immuno enzymatic method (ELISA), in which it was proved that after 12 months of storage, the colostrums silage kept feasible immunoglobulins and these were transmitted to newborns in the same proportion as the colostrum in natura. It was possible to elaborate foods such as dairy beverage and butter from the colostrum silage keeping the nutritional characteristics and microbiota containing lactic acid bacteria. The carrying out of sensory analysis reached an acceptance of 85% and purchasing intention from the assessors. Thus, it can be concluded that colostrums silage is a food of high nutritional value, microbiologically innocuous food, with immunological capability, and that it can be used in animal feeding with the need of proper legislation to enable its usability as human food. |
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2014-08-20T13:32:46Z2013-10-312014-08-20T13:32:46Z2013-05-22SAALFELD, Mara Helena. Silagem de colostro bovino: propriedades e potencialidades de usos. 2013. 98 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2013.http://guaiaca.ufpel.edu.br/handle/123456789/1212Colostrum is considered essential for newborns as a nutritional source and transfer for passive immunity. The production of colostrum above the calf intake capacity enables using the surplus as a milk replacer. One way of using it is through the anaerobic fermentation commonly called as colostrum silage. This food provides adequate weight gain to the animals; however, there is no data concerning the result of anaerobic fermentation on its elements. The aim of this study was to assess the physic-chemical, microbiological and immunological characteristics of the colostrum silage and its usage as nutritional supplement in animal feeding and its usage in the production of food for human usage. The centesimal assessment demonstrated that colostrum has nutrients in bigger amounts than the ones found in milk and that the colostrum silage kept the nutrients found in colostrum in natura, mainly the proteins (14,24%), dry matter (17,54%) and fat (5,55%), with the exception of lactose which, 30 days after fermentation was not detected anymore. It was noticed that the pH values decreased while there was an increase of lactic acid concentrations. Animals fed with colostrum silage had a weight gain at weaning of 0.7 above (p<0.05) the control animals (food with milk) which presented weight gains of 0.6 kg/day. The microbiological assessment showed that 21 days after fermentation the pathogenic bacteriae tested were not detected anymore, while there were still lactic acid bacteriae identified through sequencing the 16S rDNA as Lactobacillus (75%) and Enterococcus (25%). Identified as Lactobacillus (75%) (L. casei, L. brevis, L. rennanqily, L. crustorum) and Enterococcus (25%) (E. durans, E. faecium E. faecalis and E. mundyii). The immunoglobulins assessment in colostrum silage was carried out through the Immuno enzymatic method (ELISA), in which it was proved that after 12 months of storage, the colostrums silage kept feasible immunoglobulins and these were transmitted to newborns in the same proportion as the colostrum in natura. It was possible to elaborate foods such as dairy beverage and butter from the colostrum silage keeping the nutritional characteristics and microbiota containing lactic acid bacteria. The carrying out of sensory analysis reached an acceptance of 85% and purchasing intention from the assessors. Thus, it can be concluded that colostrums silage is a food of high nutritional value, microbiologically innocuous food, with immunological capability, and that it can be used in animal feeding with the need of proper legislation to enable its usability as human food.O colostro é considerado essencial para bovinos recém-nascidos como fonte nutricional e transferência de imunidade passiva. A produção de colostro acima da capacidade de ingestão da bezerra permite utilizar o excedente como sucedâneo lácteo. Uma das formas de utilização é através da fermentação anaeróbica popularmente chamada de silagem de colostro. Este alimento garante ao animal ganho de peso adequado, entretanto, não existem dados sobre o resultado da fermentação anaeróbica sobre seus constituintes. Objetivou-se neste estudo avaliar as características físico-químicas, microbiológicas e imunológicas da silagem de colostro e a sua utilização como suplemento nutricional em alimentação animal e o seu uso no desenvolvimento de produtos para consumo humano. A avaliação centesimal demonstrou que o colostro possui nutrientes em quantidades superiores ao leite e que a silagem de colostro manteve os nutrientes presentes no colostro in natura, principalmente proteínas (14,24%), matéria seca (17,54%) e gordura (5,55%), com exceção da lactose que, após 30 dias de fermentação, não foi mais detectada. Observou-se que os valores de pH diminuíram ao mesmo tempo que houve aumento das concentrações de ácido láctico. Animais alimentados com a silagem de colostro obtiveram ganho de peso ao desmame de 0,7Kg/dia, superiores (p<0.05) aos animais controle (alimentados com leite) que apresentaram ganhos de peso de 0,6kg/dia. A avaliação microbiológica demonstrou que após 21 dias de fermentação, as bactérias patogênicas testadas não foram mais detectadas, restando viáveis bactérias ácido lácticas que por sequenciamento da região 16S rDNA foram identificadas como Lactobacillus (75%) (L. casei, L. brevis, L. rennanqily, L. crustorum) e Enterococcus (25%) (E. durans, E. faecium, E. faecalis e E. mundyii). A avaliação das imunoglobulinas na silagem de colostro foi realizada por ELISA, ficando comprovado que após 12 meses de armazenamento a silagem de colostro manteve imunoglobulinas viáveis e estas foram transmitidas aos recém-nascidos na mesma proporção que o colostro in natura. Foi possível elaborar alimentos como, bebida láctea e manteiga, a partir da silagem de colostro, com manutenção das características nutricionais e microbiota constituída de bactérias ácido lácticas. A realização da análise sensorial obteve 85% de aprovação, com intenção de compra pelos avaliadores. Assim, pode-se concluir que a silagem de colostro é um alimento de elevado valor nutricional, microbiologicamente inócuo, com potencial imunológico, e que pode ser utilizado na alimentação animal após 21 dias de fermentação, necessitando adequações à legislação para o seu uso na alimentação humana.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em BiotecnologiaUFPelBRBiotecnologiaBiotechnologyColostrumCalvesImmunoglobulinsSilageNutritiousLactic acid BacteriaBiotecnologiaColostroBezerrosImunoglobulinasSilagemNutriçãoBactérias ácido láticasCNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMALSilagem de colostro bovino: propriedades e potencialidades de usosSilagem de colostro bovino: propriedades e potencialidades de usosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttp://lattes.cnpq.br/3053223522980282http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763076J5Pereira, Daniela Isabel Brayerhttp://lattes.cnpq.br/3382450720179401Gularte, Marcia Arochahttp://lattes.cnpq.br/5756521626319170Gandra, Eliezer Ávilahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4704972A2Leite, Fabio Pereira LeivasSaalfeld, Mara Helenainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALtese_Mara_Saalfeld.pdfapplication/pdf1507358http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1212/1/tese_Mara_Saalfeld.pdf268615e5bd86cf1b94f0f708eebbb5feMD51open accessTEXTtese_Mara_Saalfeld.pdf.txttese_Mara_Saalfeld.pdf.txtExtracted Texttext/plain154607http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1212/2/tese_Mara_Saalfeld.pdf.txtb51c3c97df30eb2ab69b71d02f0981c5MD52open accessTHUMBNAILtese_Mara_Saalfeld.pdf.jpgtese_Mara_Saalfeld.pdf.jpgGenerated Thumbnailimage/jpeg1928http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1212/3/tese_Mara_Saalfeld.pdf.jpg54fc1dcf6ca66ded75a762a802b90d0cMD53open access123456789/12122019-08-23 10:46:30.265open accessoai:guaiaca.ufpel.edu.br:123456789/1212Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-08-23T13:46:30Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Silagem de colostro bovino: propriedades e potencialidades de usos |
dc.title.alternative.eng.fl_str_mv |
Silagem de colostro bovino: propriedades e potencialidades de usos |
title |
Silagem de colostro bovino: propriedades e potencialidades de usos |
spellingShingle |
Silagem de colostro bovino: propriedades e potencialidades de usos Saalfeld, Mara Helena Biotechnology Colostrum Calves Immunoglobulins Silage Nutritious Lactic acid Bacteria Biotecnologia Colostro Bezerros Imunoglobulinas Silagem Nutrição Bactérias ácido láticas CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL |
title_short |
Silagem de colostro bovino: propriedades e potencialidades de usos |
title_full |
Silagem de colostro bovino: propriedades e potencialidades de usos |
title_fullStr |
Silagem de colostro bovino: propriedades e potencialidades de usos |
title_full_unstemmed |
Silagem de colostro bovino: propriedades e potencialidades de usos |
title_sort |
Silagem de colostro bovino: propriedades e potencialidades de usos |
author |
Saalfeld, Mara Helena |
author_facet |
Saalfeld, Mara Helena |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/3053223522980282 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763076J5 |
dc.contributor.referees1.pt_BR.fl_str_mv |
Gandra, Eliezer Ávila |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4704972A2 |
dc.contributor.advisor-co1.fl_str_mv |
Pereira, Daniela Isabel Brayer |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/3382450720179401 |
dc.contributor.advisor-co2.fl_str_mv |
Gularte, Marcia Arocha |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/5756521626319170 |
dc.contributor.advisor1.fl_str_mv |
Leite, Fabio Pereira Leivas |
dc.contributor.author.fl_str_mv |
Saalfeld, Mara Helena |
contributor_str_mv |
Pereira, Daniela Isabel Brayer Gularte, Marcia Arocha Leite, Fabio Pereira Leivas |
dc.subject.eng.fl_str_mv |
Biotechnology Colostrum Calves Immunoglobulins Silage Nutritious Lactic acid Bacteria |
topic |
Biotechnology Colostrum Calves Immunoglobulins Silage Nutritious Lactic acid Bacteria Biotecnologia Colostro Bezerros Imunoglobulinas Silagem Nutrição Bactérias ácido láticas CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL |
dc.subject.por.fl_str_mv |
Biotecnologia Colostro Bezerros Imunoglobulinas Silagem Nutrição Bactérias ácido láticas |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL |
description |
Colostrum is considered essential for newborns as a nutritional source and transfer for passive immunity. The production of colostrum above the calf intake capacity enables using the surplus as a milk replacer. One way of using it is through the anaerobic fermentation commonly called as colostrum silage. This food provides adequate weight gain to the animals; however, there is no data concerning the result of anaerobic fermentation on its elements. The aim of this study was to assess the physic-chemical, microbiological and immunological characteristics of the colostrum silage and its usage as nutritional supplement in animal feeding and its usage in the production of food for human usage. The centesimal assessment demonstrated that colostrum has nutrients in bigger amounts than the ones found in milk and that the colostrum silage kept the nutrients found in colostrum in natura, mainly the proteins (14,24%), dry matter (17,54%) and fat (5,55%), with the exception of lactose which, 30 days after fermentation was not detected anymore. It was noticed that the pH values decreased while there was an increase of lactic acid concentrations. Animals fed with colostrum silage had a weight gain at weaning of 0.7 above (p<0.05) the control animals (food with milk) which presented weight gains of 0.6 kg/day. The microbiological assessment showed that 21 days after fermentation the pathogenic bacteriae tested were not detected anymore, while there were still lactic acid bacteriae identified through sequencing the 16S rDNA as Lactobacillus (75%) and Enterococcus (25%). Identified as Lactobacillus (75%) (L. casei, L. brevis, L. rennanqily, L. crustorum) and Enterococcus (25%) (E. durans, E. faecium E. faecalis and E. mundyii). The immunoglobulins assessment in colostrum silage was carried out through the Immuno enzymatic method (ELISA), in which it was proved that after 12 months of storage, the colostrums silage kept feasible immunoglobulins and these were transmitted to newborns in the same proportion as the colostrum in natura. It was possible to elaborate foods such as dairy beverage and butter from the colostrum silage keeping the nutritional characteristics and microbiota containing lactic acid bacteria. The carrying out of sensory analysis reached an acceptance of 85% and purchasing intention from the assessors. Thus, it can be concluded that colostrums silage is a food of high nutritional value, microbiologically innocuous food, with immunological capability, and that it can be used in animal feeding with the need of proper legislation to enable its usability as human food. |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-10-31 2014-08-20T13:32:46Z |
dc.date.issued.fl_str_mv |
2013-05-22 |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:32:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SAALFELD, Mara Helena. Silagem de colostro bovino: propriedades e potencialidades de usos. 2013. 98 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2013. |
dc.identifier.uri.fl_str_mv |
http://guaiaca.ufpel.edu.br/handle/123456789/1212 |
identifier_str_mv |
SAALFELD, Mara Helena. Silagem de colostro bovino: propriedades e potencialidades de usos. 2013. 98 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2013. |
url |
http://guaiaca.ufpel.edu.br/handle/123456789/1212 |
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por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Biotecnologia |
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UFPel |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Biotecnologia |
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Universidade Federal de Pelotas |
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