Aspectos morfológicos e moleculares associados ao caráter tolerância ao encharcamento em trigo
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://guaiaca.ufpel.edu.br/handle/123456789/1167 |
Resumo: | Among the cereals, wheat (Triticum aestivum L.) is the most important crop reaching the third largest grain production in the world. Wheat is cultivated under many different environmental conditions, presenting high grain yield capacity, nutritional quality and high degree of adaptability. In the State of Rio Grande do Sul, Brazil, approximately 5.4 million hectares are considered hydromorphic soils and are mostly used for rice farming and cattle grazing, due to its low drainage capability. Annually, only one million hectares are cultivated with rice, leaving large amounts of land without cropping or cultivated with forage crops. Therefore, there is a strong challenge for breeding programs to develop genotypes capable of tolerating this environment, with a satisfactory cost/benefit ratio for farmers. In order to suppress the energy deficit provoked by hypoxic conditions, several species redirect their metabolic pathways in order to garantee the extra ATP production resulting from an increase in fermentation rates. In those species, a group of proteins that cathalyse glycolisis and phosphate sugar metabolism such as the enzyme alcohol dehydrogenase (ADH), is selectively synthesized. The objective of this work was to verify the possibility of selecting wheat genotypes tolerant to flooding stress, in the greenhouse, by using different periods of flooding conditions alterned with draining periods. Also, to estimate the degree of conservation of the ADH1 gene in some plant species. The results indicate that it is possible to detect genetic variability for the character flooding tolerance in greenhouse. The condition of eight days of flooding revealed that the genotypes BRS 120 and BRS 208 were the most tolerant and the genotypes CD 111 and BRS 194 were the most sensitive. The molecular phylogenetic analysis obtained for the alcohol dehydrogenase (ADH1), indicates that Poaceae members present a high degree of conservation. The closest species were Saccharum oficinarum and Zea mays, presenting higher nucleotide identity. The model species (Oryza sativa) presented a higher dissimilarity regarding the remaining species of the Poaceae, suggesting that this enzyme has acquired novel functions and or mutations that were selected under the flooding condition. |
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2014-08-20T13:25:41Z2010-11-032014-08-20T13:25:41Z2008-10-01BRANCO, Juliana Severo Castelo. Morphological and molecular factors associated to flooding tolerance in wheat (Triticum aestivum L.).. 2008. 92 f. Tese (Doutorado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2008.http://guaiaca.ufpel.edu.br/handle/123456789/1167Among the cereals, wheat (Triticum aestivum L.) is the most important crop reaching the third largest grain production in the world. Wheat is cultivated under many different environmental conditions, presenting high grain yield capacity, nutritional quality and high degree of adaptability. In the State of Rio Grande do Sul, Brazil, approximately 5.4 million hectares are considered hydromorphic soils and are mostly used for rice farming and cattle grazing, due to its low drainage capability. Annually, only one million hectares are cultivated with rice, leaving large amounts of land without cropping or cultivated with forage crops. Therefore, there is a strong challenge for breeding programs to develop genotypes capable of tolerating this environment, with a satisfactory cost/benefit ratio for farmers. In order to suppress the energy deficit provoked by hypoxic conditions, several species redirect their metabolic pathways in order to garantee the extra ATP production resulting from an increase in fermentation rates. In those species, a group of proteins that cathalyse glycolisis and phosphate sugar metabolism such as the enzyme alcohol dehydrogenase (ADH), is selectively synthesized. The objective of this work was to verify the possibility of selecting wheat genotypes tolerant to flooding stress, in the greenhouse, by using different periods of flooding conditions alterned with draining periods. Also, to estimate the degree of conservation of the ADH1 gene in some plant species. The results indicate that it is possible to detect genetic variability for the character flooding tolerance in greenhouse. The condition of eight days of flooding revealed that the genotypes BRS 120 and BRS 208 were the most tolerant and the genotypes CD 111 and BRS 194 were the most sensitive. The molecular phylogenetic analysis obtained for the alcohol dehydrogenase (ADH1), indicates that Poaceae members present a high degree of conservation. The closest species were Saccharum oficinarum and Zea mays, presenting higher nucleotide identity. The model species (Oryza sativa) presented a higher dissimilarity regarding the remaining species of the Poaceae, suggesting that this enzyme has acquired novel functions and or mutations that were selected under the flooding condition.Entre os cereais, o trigo (Triticum aestivum L.) é a mais importante cultura, alcançando a terceira maior produção de grãos no mundo sobrepujado apenas pelo milho e arroz. O trigo é cultivado sob diversas condições ambientais, apresentando alta capacidade de produção de grãos, qualidade nutricional e alto grau de adaptabilidade. No Estado do Rio Grande do Sul, aproximadamente 5,4 milhões de hectares são considerados solos hidromórficos e são principalmente utilizados para cultivo de arroz e pastagem, devido a sua baixa capacidade de drenagem. Anualmente, apenas um milhão de hectares está sendo utilizado para o cultivo de arroz, deixando grandes quantidades de terras para o cultivo de culturas forrageiras. Portanto, existe um forte desafio para programas de melhoramento no desenvolvimento de genótipos capazes de tolerar esse ambiente, com uma boa relação custo/benefício para os agricultores. A fim de suprimir o déficit energético provocado pelas condições de hipoxia, várias espécies redirecionam suas vias metabólicas, a fim de garantir a produção extra ATP resultantes do aumento das taxas de fermentação. Nessas espécies, um grupo de proteínas que catalisam a glicólise e metabolismo de açúcares, como a enzima álcool desidrogenase (ADH), é sintetizada seletivamente. O objetivo deste trabalho foi verificar a possibilidade de seleção de genótipos de trigo tolerantes ao estresse por alagamento, em casa de vegetação, utilizando diferentes períodos de alagamento intercalados com drenagem. Além disso, estimar o grau de conservação do gene ADH1 em algumas espécies de plantas. Os resultados indicam que é possível a detecção de variabilidade genética para o caráter tolerância ao alagamento em casa de vegetação. A condição de oito dias de alagamento revelou que os genótipos BRS 120 e BRS 208 foram as mais tolerantes e os genótipos CD 111 e BRS 194 foram as mais sensíveis. A análise filogenética molecular obtida para o álcool desidrogenase (ADH1), indica que os membros Poaceae apresentaram um elevado grau de conservação. As espécies mais próximas foram Saccharum oficinarum e Zea mays, apresentando maior identidade de nucleotídeos. A espécie modelo para plantas monocotiledôneas (Oryza sativa) apresenta uma maior dissimilaridade quanto ao restante das espécies de Poaceae, sugerindo que esta enzima tenha adquirido níveis de funções ou mutações que foram selecionadas sob a condição alagamento.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em AgronomiaUFPelBRFaculdade de Agronomia Eliseu MacielEncharcamentoAlcool desidrogenaseADHTriticum aestivum LFloodingAlcohol dehydrogenaseCNPQ::CIENCIAS AGRARIAS::AGRONOMIAAspectos morfológicos e moleculares associados ao caráter tolerância ao encharcamento em trigoMorphological and molecular factors associated to flooding tolerance in wheat (Triticum aestivum L.).info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780819H4Carvalho, Fernando Irajá Félix dehttp://lattes.cnpq.br/4259802666999757Oliveira, Antônio Costa deBranco, Juliana Severo Casteloinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALTese_Juliana_Severo_Castelo_Branco.pdfapplication/pdf7339681http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1167/1/Tese_Juliana_Severo_Castelo_Branco.pdf5c86995be26b0fd8b4d1fda6177461ecMD51open accessTEXTTese_Juliana_Severo_Castelo_Branco.pdf.txtTese_Juliana_Severo_Castelo_Branco.pdf.txtExtracted Texttext/plain137321http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1167/2/Tese_Juliana_Severo_Castelo_Branco.pdf.txtbc1db780e548fba85b76cc0a322b81faMD52open accessTHUMBNAILTese_Juliana_Severo_Castelo_Branco.pdf.jpgTese_Juliana_Severo_Castelo_Branco.pdf.jpgGenerated Thumbnailimage/jpeg1275http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1167/3/Tese_Juliana_Severo_Castelo_Branco.pdf.jpg0e94eed1a01e2a021c1b689d833dd360MD53open access123456789/11672023-07-17 23:06:49.007open accessoai:guaiaca.ufpel.edu.br:123456789/1167Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2023-07-18T02:06:49Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Aspectos morfológicos e moleculares associados ao caráter tolerância ao encharcamento em trigo |
dc.title.alternative.eng.fl_str_mv |
Morphological and molecular factors associated to flooding tolerance in wheat (Triticum aestivum L.). |
title |
Aspectos morfológicos e moleculares associados ao caráter tolerância ao encharcamento em trigo |
spellingShingle |
Aspectos morfológicos e moleculares associados ao caráter tolerância ao encharcamento em trigo Branco, Juliana Severo Castelo Encharcamento Alcool desidrogenase ADH Triticum aestivum L Flooding Alcohol dehydrogenase CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Aspectos morfológicos e moleculares associados ao caráter tolerância ao encharcamento em trigo |
title_full |
Aspectos morfológicos e moleculares associados ao caráter tolerância ao encharcamento em trigo |
title_fullStr |
Aspectos morfológicos e moleculares associados ao caráter tolerância ao encharcamento em trigo |
title_full_unstemmed |
Aspectos morfológicos e moleculares associados ao caráter tolerância ao encharcamento em trigo |
title_sort |
Aspectos morfológicos e moleculares associados ao caráter tolerância ao encharcamento em trigo |
author |
Branco, Juliana Severo Castelo |
author_facet |
Branco, Juliana Severo Castelo |
author_role |
author |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780819H4 |
dc.contributor.advisor-co1.fl_str_mv |
Carvalho, Fernando Irajá Félix de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/4259802666999757 |
dc.contributor.advisor1.fl_str_mv |
Oliveira, Antônio Costa de |
dc.contributor.author.fl_str_mv |
Branco, Juliana Severo Castelo |
contributor_str_mv |
Carvalho, Fernando Irajá Félix de Oliveira, Antônio Costa de |
dc.subject.por.fl_str_mv |
Encharcamento Alcool desidrogenase ADH Triticum aestivum L |
topic |
Encharcamento Alcool desidrogenase ADH Triticum aestivum L Flooding Alcohol dehydrogenase CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Flooding Alcohol dehydrogenase |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
Among the cereals, wheat (Triticum aestivum L.) is the most important crop reaching the third largest grain production in the world. Wheat is cultivated under many different environmental conditions, presenting high grain yield capacity, nutritional quality and high degree of adaptability. In the State of Rio Grande do Sul, Brazil, approximately 5.4 million hectares are considered hydromorphic soils and are mostly used for rice farming and cattle grazing, due to its low drainage capability. Annually, only one million hectares are cultivated with rice, leaving large amounts of land without cropping or cultivated with forage crops. Therefore, there is a strong challenge for breeding programs to develop genotypes capable of tolerating this environment, with a satisfactory cost/benefit ratio for farmers. In order to suppress the energy deficit provoked by hypoxic conditions, several species redirect their metabolic pathways in order to garantee the extra ATP production resulting from an increase in fermentation rates. In those species, a group of proteins that cathalyse glycolisis and phosphate sugar metabolism such as the enzyme alcohol dehydrogenase (ADH), is selectively synthesized. The objective of this work was to verify the possibility of selecting wheat genotypes tolerant to flooding stress, in the greenhouse, by using different periods of flooding conditions alterned with draining periods. Also, to estimate the degree of conservation of the ADH1 gene in some plant species. The results indicate that it is possible to detect genetic variability for the character flooding tolerance in greenhouse. The condition of eight days of flooding revealed that the genotypes BRS 120 and BRS 208 were the most tolerant and the genotypes CD 111 and BRS 194 were the most sensitive. The molecular phylogenetic analysis obtained for the alcohol dehydrogenase (ADH1), indicates that Poaceae members present a high degree of conservation. The closest species were Saccharum oficinarum and Zea mays, presenting higher nucleotide identity. The model species (Oryza sativa) presented a higher dissimilarity regarding the remaining species of the Poaceae, suggesting that this enzyme has acquired novel functions and or mutations that were selected under the flooding condition. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-10-01 |
dc.date.available.fl_str_mv |
2010-11-03 2014-08-20T13:25:41Z |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:25:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
BRANCO, Juliana Severo Castelo. Morphological and molecular factors associated to flooding tolerance in wheat (Triticum aestivum L.).. 2008. 92 f. Tese (Doutorado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2008. |
dc.identifier.uri.fl_str_mv |
http://guaiaca.ufpel.edu.br/handle/123456789/1167 |
identifier_str_mv |
BRANCO, Juliana Severo Castelo. Morphological and molecular factors associated to flooding tolerance in wheat (Triticum aestivum L.).. 2008. 92 f. Tese (Doutorado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2008. |
url |
http://guaiaca.ufpel.edu.br/handle/123456789/1167 |
dc.language.iso.fl_str_mv |
por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Agronomia |
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UFPel |
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BR |
dc.publisher.department.fl_str_mv |
Faculdade de Agronomia Eliseu Maciel |
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Universidade Federal de Pelotas |
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