Micropropagação fotoautotrófica de amoreira-preta (Rubus spp.) e framboeseira (Rubus idaeus L.) com a utilização de luz natural.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://guaiaca.ufpel.edu.br/handle/123456789/2112 |
Resumo: | The success of mass micropropagation of fruit trees may be reached by using plant tissues culture techniques, since this has showed efficient results on seedlings production with high quality and health. However, for the commercial viability of micropropagation application in the field of horticulture and how this might compete with traditional methods of propagation (cuttings, etc.), it is necessary to decrease production costs. Therefore, the development of photo-autotrophic micropropagation systems (production of micropropagules in sugar-free medium under environmental conditions that promote photosynthesis of the culture) with natural light appear as a possibility to improve the efficiency of micropropagation and to reduce costs. This research aimed the photo-autotrophic multiplication of blackberry (Rubus spp.) cultivar Xavante and raspberry (Rubus idaeus L.) cultivars Batum and Heritage. Preliminary experiments was carried out to define the constitution of culture medium that provides better results, as on multiplication as on in vitro rooting of blackberry and raspberry, under conventional conditions of micropropagation. Then, using the best constitution of culture medium, it was done the study of the photo-autotrophic multiplication by using natural light. The MS medium enriched with BAP at 13 µM was the more efficient treatment on in vitro multiplication of leaves of blackberry Xavante and raspberries Batum and Heritage , inducing a higher number of leaves, shoots and buds. The best rooting condition for explants of the blackberry Xavante was reached by keeping the explants in WPM enriched with 2,5 µM AIB for a week followed by a regulator-free medium growth. Nevertheless, for raspberry Batum rooting, it is necessary the addition of 6,5 µM AIB. Under photo- autotrophic conditions, the aluminum foil was the best sealing material for the flasks. Regarding to in vitro multiplication for blackberry cultivar Xavante the best growth local was in greenhouse with the addition of 22 g L -1 sucrose to the medium; and for raspberry Batum was at 11,5 g L-1 sucrose but kept in growth room. For in vitro rooting of raspberry Batum cotton was the best sealing material, growth room and sugar-free medium were the best condition having a higher rooting percentage and root numbers per explants. |
id |
UFPL_cce9db27ba484eceb764e696e7aeb11f |
---|---|
oai_identifier_str |
oai:guaiaca.ufpel.edu.br:123456789/2112 |
network_acronym_str |
UFPL |
network_name_str |
Repositório Institucional da UFPel - Guaiaca |
repository_id_str |
|
spelling |
http://lattes.cnpq.br/0179478305889214http://lattes.cnpq.br/4400873265650402Antunes, Luís Eduardo Corrêahttp://lattes.cnpq.br/8683376495079788Schuch, Márcia WulffLeitzke, Luciane Nolasco2014-08-20T14:22:04Z2008-05-142014-08-20T14:22:04Z2006-03-05LEITZKE, Luciane Nolasco. Photo-autotrophic micropropagation of blackberry (Rubus spp.) and raspberry (Rubus idaeus L.) by using natural light.. 2006. 94 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2006.http://guaiaca.ufpel.edu.br/handle/123456789/2112The success of mass micropropagation of fruit trees may be reached by using plant tissues culture techniques, since this has showed efficient results on seedlings production with high quality and health. However, for the commercial viability of micropropagation application in the field of horticulture and how this might compete with traditional methods of propagation (cuttings, etc.), it is necessary to decrease production costs. Therefore, the development of photo-autotrophic micropropagation systems (production of micropropagules in sugar-free medium under environmental conditions that promote photosynthesis of the culture) with natural light appear as a possibility to improve the efficiency of micropropagation and to reduce costs. This research aimed the photo-autotrophic multiplication of blackberry (Rubus spp.) cultivar Xavante and raspberry (Rubus idaeus L.) cultivars Batum and Heritage. Preliminary experiments was carried out to define the constitution of culture medium that provides better results, as on multiplication as on in vitro rooting of blackberry and raspberry, under conventional conditions of micropropagation. Then, using the best constitution of culture medium, it was done the study of the photo-autotrophic multiplication by using natural light. The MS medium enriched with BAP at 13 µM was the more efficient treatment on in vitro multiplication of leaves of blackberry Xavante and raspberries Batum and Heritage , inducing a higher number of leaves, shoots and buds. The best rooting condition for explants of the blackberry Xavante was reached by keeping the explants in WPM enriched with 2,5 µM AIB for a week followed by a regulator-free medium growth. Nevertheless, for raspberry Batum rooting, it is necessary the addition of 6,5 µM AIB. Under photo- autotrophic conditions, the aluminum foil was the best sealing material for the flasks. Regarding to in vitro multiplication for blackberry cultivar Xavante the best growth local was in greenhouse with the addition of 22 g L -1 sucrose to the medium; and for raspberry Batum was at 11,5 g L-1 sucrose but kept in growth room. For in vitro rooting of raspberry Batum cotton was the best sealing material, growth room and sugar-free medium were the best condition having a higher rooting percentage and root numbers per explants.A cultura de tecidos é uma técnica que proporciona com sucesso a micropropagação massal de frutíferas e que já vem sendo utilizada com eficientes resultados para a produção de mudas sadias com alta qualidade. Entretanto, para que a aplicação da micropropagação na fruticultura torne-se viável comercialmente e possa competir com métodos tradicionais de propagação (estaquia, etc), é necessária a redução do custo de produção. Diante disso, o desenvolvimento de sistemas de micropropagação fotoautotrófica (produção de micropropágulos sem adição de sacarose no meio de cultura e sob condições ambientais que promovam a fotossíntese na planta) com o uso de luz natural surge como possibilidade que apresenta potencial para aumentar a eficiência da micropropagação e auxiliar na redução de seu custo. Assim, este trabalho teve como objetivo a multiplicação fotoautotrófica de amoreira-preta (Rubus spp.) cv. Xavante e de framboeseira (Rubus idaeus L.) cvs. Batum e Heritage. Dessa forma, foram realizados os estudos preliminares a fim de definir a constituição do meio de cultura que propicie os melhores resultados, tanto na multiplicação como no enraizamento in vitro de amorapreta e framboesa, sob condições convencionais de micropropagação. A partir daí, foi realizado o estudo da micropropagação fotoautotrófico com o uso da luz natural, utilizando a constituição do meio de cultura que propiciou os melhores resultados. Pelos resultados obtidos, conclui-se que o meio MS adicionado de BAP na concentração de 13µM é o tratamento mais eficiente na multiplicação in vitro de explantes com folhas de amoreira-preta Xavante e framboeseira Batum e Heritage , induzindo maior número de folhas, brotações e gemas. Para o enraizamento in vitro de amoreira-preta Xavante , o meio WPM adicionado de 2,5µM AIB e mantido por uma semana, seguido do cultivo em meio livre de regulador é o melhor meio de enraizamento; para framboeseira Batum , é necessária a adição de 6,5µM de AIB. Em condições fotoautotróficas o alumínio é o melhor modo de vedação dos frascos de cultivo. Para a multiplicação in vitro de amoreira-preta cv. Xavante , o melhor local de cultivo é a casa de vegetação e a adição de 22 g L -1 de sacarose no meio de cultura e de 11,5g L-1 para framboeseira Batum , mantida na sala de crescimento. Para o enraizamento in vitro de framboeseira cv. Batum, o algodão é o melhor modo de vedação dos frascos de cultivo; o melhor local de cultivo é a sala de crescimento, sem a adição de sacarose no meio de cultura, obtendo-se maior porcentagem de enraizamento, número de raízes por explante.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em AgronomiaUFPelBRFaculdade de Agronomia Eliseu MacielProdução de mudasXavanteHeritageCultura de tecidosBatumPlant tissue cultureBatumHeritageXavanteSeedlings productionCNPQ::CIENCIAS AGRARIAS::AGRONOMIAMicropropagação fotoautotrófica de amoreira-preta (Rubus spp.) e framboeseira (Rubus idaeus L.) com a utilização de luz natural.Photo-autotrophic micropropagation of blackberry (Rubus spp.) and raspberry (Rubus idaeus L.) by using natural light.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALDissertacao_Luciane_Leitzke.pdfapplication/pdf527181http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2112/1/Dissertacao_Luciane_Leitzke.pdf1bd073c7e15cf594e5e31a8d64217660MD51open accessTEXTDissertacao_Luciane_Leitzke.pdf.txtDissertacao_Luciane_Leitzke.pdf.txtExtracted Texttext/plain145247http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2112/2/Dissertacao_Luciane_Leitzke.pdf.txt6917dd7144ef19f6c1146ff259dbc256MD52open accessTHUMBNAILDissertacao_Luciane_Leitzke.pdf.jpgDissertacao_Luciane_Leitzke.pdf.jpgGenerated Thumbnailimage/jpeg1467http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2112/3/Dissertacao_Luciane_Leitzke.pdf.jpg1bf9bd03250b0fae84a028c93098ce96MD53open access123456789/21122023-05-31 11:59:37.4open accessoai:guaiaca.ufpel.edu.br:123456789/2112Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2023-05-31T14:59:37Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Micropropagação fotoautotrófica de amoreira-preta (Rubus spp.) e framboeseira (Rubus idaeus L.) com a utilização de luz natural. |
dc.title.alternative.eng.fl_str_mv |
Photo-autotrophic micropropagation of blackberry (Rubus spp.) and raspberry (Rubus idaeus L.) by using natural light. |
title |
Micropropagação fotoautotrófica de amoreira-preta (Rubus spp.) e framboeseira (Rubus idaeus L.) com a utilização de luz natural. |
spellingShingle |
Micropropagação fotoautotrófica de amoreira-preta (Rubus spp.) e framboeseira (Rubus idaeus L.) com a utilização de luz natural. Leitzke, Luciane Nolasco Produção de mudas Xavante Heritage Cultura de tecidos Batum Plant tissue culture Batum Heritage Xavante Seedlings production CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Micropropagação fotoautotrófica de amoreira-preta (Rubus spp.) e framboeseira (Rubus idaeus L.) com a utilização de luz natural. |
title_full |
Micropropagação fotoautotrófica de amoreira-preta (Rubus spp.) e framboeseira (Rubus idaeus L.) com a utilização de luz natural. |
title_fullStr |
Micropropagação fotoautotrófica de amoreira-preta (Rubus spp.) e framboeseira (Rubus idaeus L.) com a utilização de luz natural. |
title_full_unstemmed |
Micropropagação fotoautotrófica de amoreira-preta (Rubus spp.) e framboeseira (Rubus idaeus L.) com a utilização de luz natural. |
title_sort |
Micropropagação fotoautotrófica de amoreira-preta (Rubus spp.) e framboeseira (Rubus idaeus L.) com a utilização de luz natural. |
author |
Leitzke, Luciane Nolasco |
author_facet |
Leitzke, Luciane Nolasco |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/0179478305889214 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/4400873265650402 |
dc.contributor.advisor-co1.fl_str_mv |
Antunes, Luís Eduardo Corrêa |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/8683376495079788 |
dc.contributor.advisor1.fl_str_mv |
Schuch, Márcia Wulff |
dc.contributor.author.fl_str_mv |
Leitzke, Luciane Nolasco |
contributor_str_mv |
Antunes, Luís Eduardo Corrêa Schuch, Márcia Wulff |
dc.subject.por.fl_str_mv |
Produção de mudas Xavante Heritage Cultura de tecidos Batum |
topic |
Produção de mudas Xavante Heritage Cultura de tecidos Batum Plant tissue culture Batum Heritage Xavante Seedlings production CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Plant tissue culture Batum Heritage Xavante Seedlings production |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
The success of mass micropropagation of fruit trees may be reached by using plant tissues culture techniques, since this has showed efficient results on seedlings production with high quality and health. However, for the commercial viability of micropropagation application in the field of horticulture and how this might compete with traditional methods of propagation (cuttings, etc.), it is necessary to decrease production costs. Therefore, the development of photo-autotrophic micropropagation systems (production of micropropagules in sugar-free medium under environmental conditions that promote photosynthesis of the culture) with natural light appear as a possibility to improve the efficiency of micropropagation and to reduce costs. This research aimed the photo-autotrophic multiplication of blackberry (Rubus spp.) cultivar Xavante and raspberry (Rubus idaeus L.) cultivars Batum and Heritage. Preliminary experiments was carried out to define the constitution of culture medium that provides better results, as on multiplication as on in vitro rooting of blackberry and raspberry, under conventional conditions of micropropagation. Then, using the best constitution of culture medium, it was done the study of the photo-autotrophic multiplication by using natural light. The MS medium enriched with BAP at 13 µM was the more efficient treatment on in vitro multiplication of leaves of blackberry Xavante and raspberries Batum and Heritage , inducing a higher number of leaves, shoots and buds. The best rooting condition for explants of the blackberry Xavante was reached by keeping the explants in WPM enriched with 2,5 µM AIB for a week followed by a regulator-free medium growth. Nevertheless, for raspberry Batum rooting, it is necessary the addition of 6,5 µM AIB. Under photo- autotrophic conditions, the aluminum foil was the best sealing material for the flasks. Regarding to in vitro multiplication for blackberry cultivar Xavante the best growth local was in greenhouse with the addition of 22 g L -1 sucrose to the medium; and for raspberry Batum was at 11,5 g L-1 sucrose but kept in growth room. For in vitro rooting of raspberry Batum cotton was the best sealing material, growth room and sugar-free medium were the best condition having a higher rooting percentage and root numbers per explants. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-03-05 |
dc.date.available.fl_str_mv |
2008-05-14 2014-08-20T14:22:04Z |
dc.date.accessioned.fl_str_mv |
2014-08-20T14:22:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LEITZKE, Luciane Nolasco. Photo-autotrophic micropropagation of blackberry (Rubus spp.) and raspberry (Rubus idaeus L.) by using natural light.. 2006. 94 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2006. |
dc.identifier.uri.fl_str_mv |
http://guaiaca.ufpel.edu.br/handle/123456789/2112 |
identifier_str_mv |
LEITZKE, Luciane Nolasco. Photo-autotrophic micropropagation of blackberry (Rubus spp.) and raspberry (Rubus idaeus L.) by using natural light.. 2006. 94 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2006. |
url |
http://guaiaca.ufpel.edu.br/handle/123456789/2112 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Agronomia |
dc.publisher.initials.fl_str_mv |
UFPel |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Faculdade de Agronomia Eliseu Maciel |
publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFPel - Guaiaca instname:Universidade Federal de Pelotas (UFPEL) instacron:UFPEL |
instname_str |
Universidade Federal de Pelotas (UFPEL) |
instacron_str |
UFPEL |
institution |
UFPEL |
reponame_str |
Repositório Institucional da UFPel - Guaiaca |
collection |
Repositório Institucional da UFPel - Guaiaca |
bitstream.url.fl_str_mv |
http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2112/1/Dissertacao_Luciane_Leitzke.pdf http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2112/2/Dissertacao_Luciane_Leitzke.pdf.txt http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2112/3/Dissertacao_Luciane_Leitzke.pdf.jpg |
bitstream.checksum.fl_str_mv |
1bd073c7e15cf594e5e31a8d64217660 6917dd7144ef19f6c1146ff259dbc256 1bf9bd03250b0fae84a028c93098ce96 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL) |
repository.mail.fl_str_mv |
rippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.br |
_version_ |
1801846927905521664 |