Congelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidade
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://guaiaca.ufpel.edu.br/handle/123456789/1229 |
Resumo: | Although boar semen is available in the frozen format since 1975, its use has been restricted to very specific situations for many reasons: it requires spermatozoa concentration per dose twice to three times higher than that for cooled semen; semen processing is labor intensive; and farrowing rate and litter size are reduced. Thus, most of the artificial inseminations in swine are performed at the day of semen collection or at most at the following day, using liquid semen conditioned between 15 and 18 ºC. The aims of this thesis were: to evaluate distinct extenders for cooling semen, methods for freezing, centrifugation temperatures, the use of amides as penetrating cryoprotectants and of low density lipoprotein as nonpenetrating cryoprotectants, and the presence of specific protein factors in seminal plasma in the results that could be associated with boar semen freezability. Five experiments have been executed: four with evaluations in vitro (motility and cell membrane integrity); and one with fertilization in vivo. The control treatment was the freezing with glycerol at 3% concentration. The semen processing method that provide more efficient results consisted of semen cooling during 120 min up to a centrifugation temperature of 15 °C. We also identified a 26 kDa factor in the seminal plasma that is associated with maintenance of the integrity of the sperm cell membrane post-thawing. Additionally, parameters of semen quality in vitro with the use of dimetilacetamide at 5% were better than those observed with the most used penetrating cryoprotectant (glycerol 3%) as control, although no difference between those cryoprotectants was observed in vivo. This study demonstrated the association of components in the seminal plasma with the sperm quality, and presenting an alternative protocol of semen freezing-thawed boar semen based in the use of dimetilacetamide at 5%. |
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2014-08-20T13:32:49Z2007-10-232014-08-20T13:32:49Z2007-02-27BIANCHI, Ivan. Freezing of boar semen: study of penetrating and nonpenetrating cryoprotectants, freezing methods and freezability molecular marker. 2007. 95 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2007.http://guaiaca.ufpel.edu.br/handle/123456789/1229Although boar semen is available in the frozen format since 1975, its use has been restricted to very specific situations for many reasons: it requires spermatozoa concentration per dose twice to three times higher than that for cooled semen; semen processing is labor intensive; and farrowing rate and litter size are reduced. Thus, most of the artificial inseminations in swine are performed at the day of semen collection or at most at the following day, using liquid semen conditioned between 15 and 18 ºC. The aims of this thesis were: to evaluate distinct extenders for cooling semen, methods for freezing, centrifugation temperatures, the use of amides as penetrating cryoprotectants and of low density lipoprotein as nonpenetrating cryoprotectants, and the presence of specific protein factors in seminal plasma in the results that could be associated with boar semen freezability. Five experiments have been executed: four with evaluations in vitro (motility and cell membrane integrity); and one with fertilization in vivo. The control treatment was the freezing with glycerol at 3% concentration. The semen processing method that provide more efficient results consisted of semen cooling during 120 min up to a centrifugation temperature of 15 °C. We also identified a 26 kDa factor in the seminal plasma that is associated with maintenance of the integrity of the sperm cell membrane post-thawing. Additionally, parameters of semen quality in vitro with the use of dimetilacetamide at 5% were better than those observed with the most used penetrating cryoprotectant (glycerol 3%) as control, although no difference between those cryoprotectants was observed in vivo. This study demonstrated the association of components in the seminal plasma with the sperm quality, and presenting an alternative protocol of semen freezing-thawed boar semen based in the use of dimetilacetamide at 5%.Apesar do sêmen suíno congelado estar disponível comercialmente deste 1975, o seu uso tem ocorrido somente em ocasiões específicas, pois, em relação ao uso de sêmen refrigerado, requer duas a três vezes maior número de espermatozóides por dose, o processamento do sêmen é trabalhoso, o tamanho da leitegada é diminuído em um a três leitões por parto e a taxa de parição é menor. Dessa forma, a grande maioria das inseminações artificiais nessa espécie utiliza sêmen diluído e acondicionado no estado líquido entre 15 e 18 ºC. Os objetivos desta tese foram: avaliar diferentes diluidores de resfriamento, metodologias de congelamento, temperaturas de centrifugação, uso de crioprotetores intracelulares a base de amidas e extracelulares baseados em lipoproteína de baixa densidade, além do estudo de fatores protéicos presentes no plasma seminal nos resultados de avaliações in vitro e in vivo de sêmen suíno congelado-descongelado. Foram executados cinco experimentos com avaliações da qualidade do sêmen congeladodescongelado, quatro experimentos com avaliações in vitro (motilidade e integridade de membrana) e um experimento com fertilização in vivo. O tratamento utilizado como controle foi o congelamento com glicerol na concentração de 3%. A melhor curva de resfriamento após a coleta até 15 °C, foi atingida com o tempo de 120 min. Foi identificado um fator de 26 kDa no plasma seminal, cuja ausência proporcionou melhores resultados de integridade de membrana plasmática após o descongelamento. O uso do crioprotetor intracelular dimetilacetamida na concentração de 5%, proporcionou nas avaliações in vitro, resultados superiores ao tratamento controle (glicerol 3%), não diferindo na avaliação in vivo. O presente estudo demonstrou a relação de componentes do plasma seminal com a qualidade espermática, além de apresentar um novo protocolo de congelamentodescongelamento de sêmen suíno baseado no uso de dimetilacetamida 5%.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em BiotecnologiaUFPelBRBiotecnologiaBiotechnologyVeterinaryAmidesFrozen semenSwineCryopreservationGlycerolBiotecnologiaVeterináriaAmidasSuínosSêmen congeladoCriopreservaçãoGlicerolCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMALCongelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidadeFreezing of boar semen: study of penetrating and nonpenetrating cryoprotectants, freezing methods and freezability molecular markerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768000E7http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796653E9Deschamps, João Carloshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783390T8Lucia Júnior, Thomazhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793234U8Corrêa, Márcio NunesBianchi, Ivaninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALtese_ivan_bianchi.pdfapplication/pdf495717http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1229/1/tese_ivan_bianchi.pdf66978c47d9c193a45f85b5cad5be6c3fMD51open accessTEXTtese_ivan_bianchi.pdf.txttese_ivan_bianchi.pdf.txtExtracted Texttext/plain165895http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1229/2/tese_ivan_bianchi.pdf.txt9def6508a66a787d4c705164fb6bfb08MD52open accessTHUMBNAILtese_ivan_bianchi.pdf.jpgtese_ivan_bianchi.pdf.jpgGenerated Thumbnailimage/jpeg2071http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1229/3/tese_ivan_bianchi.pdf.jpg066c76efddeaba15eeb7cd4db3b2bbd4MD53open access123456789/12292019-08-23 10:06:50.568open accessoai:guaiaca.ufpel.edu.br:123456789/1229Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-08-23T13:06:50Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Congelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidade |
dc.title.alternative.eng.fl_str_mv |
Freezing of boar semen: study of penetrating and nonpenetrating cryoprotectants, freezing methods and freezability molecular marker |
title |
Congelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidade |
spellingShingle |
Congelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidade Bianchi, Ivan Biotechnology Veterinary Amides Frozen semen Swine Cryopreservation Glycerol Biotecnologia Veterinária Amidas Suínos Sêmen congelado Criopreservação Glicerol CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL |
title_short |
Congelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidade |
title_full |
Congelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidade |
title_fullStr |
Congelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidade |
title_full_unstemmed |
Congelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidade |
title_sort |
Congelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidade |
author |
Bianchi, Ivan |
author_facet |
Bianchi, Ivan |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768000E7 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796653E9 |
dc.contributor.advisor-co1.fl_str_mv |
Deschamps, João Carlos |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783390T8 |
dc.contributor.advisor-co2.fl_str_mv |
Lucia Júnior, Thomaz |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793234U8 |
dc.contributor.advisor1.fl_str_mv |
Corrêa, Márcio Nunes |
dc.contributor.author.fl_str_mv |
Bianchi, Ivan |
contributor_str_mv |
Deschamps, João Carlos Lucia Júnior, Thomaz Corrêa, Márcio Nunes |
dc.subject.eng.fl_str_mv |
Biotechnology Veterinary Amides Frozen semen Swine Cryopreservation Glycerol |
topic |
Biotechnology Veterinary Amides Frozen semen Swine Cryopreservation Glycerol Biotecnologia Veterinária Amidas Suínos Sêmen congelado Criopreservação Glicerol CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL |
dc.subject.por.fl_str_mv |
Biotecnologia Veterinária Amidas Suínos Sêmen congelado Criopreservação Glicerol |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL |
description |
Although boar semen is available in the frozen format since 1975, its use has been restricted to very specific situations for many reasons: it requires spermatozoa concentration per dose twice to three times higher than that for cooled semen; semen processing is labor intensive; and farrowing rate and litter size are reduced. Thus, most of the artificial inseminations in swine are performed at the day of semen collection or at most at the following day, using liquid semen conditioned between 15 and 18 ºC. The aims of this thesis were: to evaluate distinct extenders for cooling semen, methods for freezing, centrifugation temperatures, the use of amides as penetrating cryoprotectants and of low density lipoprotein as nonpenetrating cryoprotectants, and the presence of specific protein factors in seminal plasma in the results that could be associated with boar semen freezability. Five experiments have been executed: four with evaluations in vitro (motility and cell membrane integrity); and one with fertilization in vivo. The control treatment was the freezing with glycerol at 3% concentration. The semen processing method that provide more efficient results consisted of semen cooling during 120 min up to a centrifugation temperature of 15 °C. We also identified a 26 kDa factor in the seminal plasma that is associated with maintenance of the integrity of the sperm cell membrane post-thawing. Additionally, parameters of semen quality in vitro with the use of dimetilacetamide at 5% were better than those observed with the most used penetrating cryoprotectant (glycerol 3%) as control, although no difference between those cryoprotectants was observed in vivo. This study demonstrated the association of components in the seminal plasma with the sperm quality, and presenting an alternative protocol of semen freezing-thawed boar semen based in the use of dimetilacetamide at 5%. |
publishDate |
2007 |
dc.date.available.fl_str_mv |
2007-10-23 2014-08-20T13:32:49Z |
dc.date.issued.fl_str_mv |
2007-02-27 |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:32:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BIANCHI, Ivan. Freezing of boar semen: study of penetrating and nonpenetrating cryoprotectants, freezing methods and freezability molecular marker. 2007. 95 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2007. |
dc.identifier.uri.fl_str_mv |
http://guaiaca.ufpel.edu.br/handle/123456789/1229 |
identifier_str_mv |
BIANCHI, Ivan. Freezing of boar semen: study of penetrating and nonpenetrating cryoprotectants, freezing methods and freezability molecular marker. 2007. 95 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2007. |
url |
http://guaiaca.ufpel.edu.br/handle/123456789/1229 |
dc.language.iso.fl_str_mv |
por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Biotecnologia |
dc.publisher.initials.fl_str_mv |
UFPel |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Biotecnologia |
publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
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