Micropropagação e estresse oxidativo de pereira e marmeleiro cultivados em meio contendo alumínio

• Autor(a) principal: Ribeiro, Mirian de Farias
• Data de Publicação: 2010
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFPel - Guaiaca
• Texto Completo: http://repositorio.ufpel.edu.br/handle/ri/2037
• topic: Multiplicação in vitro; Enraizamento in vitro; Solos ácidos; Toxicidade; In vitro multiplication; In vitro rooting; Acidic soils; Toxicity; CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL

The objective of this work was to evaluate the effect of aluminum (Al) on in vitro multiplication and rooting of pear and quince. The explants were nodal segments of pear and quince in vitro cultivated. The culture medium to pear was QL modificated by Leblay and to quince was MS, both added of Al to 0 (pH 5,7, control), 0, 15, 30, 45 e 60 mg L-1 (pH 4,0) put in jars with 1 g of cotton for multiplication and 3 g of vermiculite for rooting and 30 mL of medium. The trial had four replicates and five explants each. At 60 days the percentage of shoots, length and number of shoots, fresh and dry mass and catalase (CAT) and ascorbate peroxidase (APX) activity in pear were assessed. At 45 days the percentage of rooting, length and number of root, fresh and dry mass of shoots and roots, CAT, APX and superoxide dismutase (SOD) activity in pear and nutrients content were assessed. In the multiplication, the quince had 100% of shoot, but decreased the number of shoots. The length of shoots, fresh and dry mass decreased in MC but increased in BA 29 . The pear had high percentage of shoots. The number of shoot was higher for Conference . The length of shoots, fresh and dry mass was increased at Al. CAT activity in Conference was increased at 30 mg L-1 of Al and in Abate Fetel was increased at 30 and 45 mg L-1 of Al. APX activity in Conference was increased at 30 and 60 mg L-1 of Al and in Abate Fetel was increased at 30 mg L-1 of Al. In the rooting, the percentage of rooting, lenght and number of roots was higher for quince. The fresh mass of shoot was higher for Conference and for 30 mg L-1 of Al. The fresh mass of root was higher for BA 29 at 15 mg L-1 of Al. The dry mass of shoot was higher for Conference and for 15 and 30 mg L-1 of Al. The dry mass of root was higher for BA 29 and for 15 mg L-1 of Al. The nutrient content increased at aluminum. In the Abate Fetel the CAT and APX activity was increased and the SOD activity was decreased at aluminum. On the other hand, in the Conference the CAT activity was decreased at aluminum, the APX was decreased only at 45 mg L-1 of Al and the SOD activity was increased. As conclusion, the cultivars answered of different form at Al and, in some parameters, the presence of this metal was beneficial.