Transferability of microsatellite markers to study the genetic diversity of the copaiba
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Publication Date: | 2024 |
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Format: | Article |
Language: | por |
Source: | Revista de Ciências Agrárias (Belém. Online) |
Download full: | https://ajaes.ufra.edu.br/index.php/ajaes/article/view/3611 |
Summary: | The copaiba (Copaifera multijuga Hayne) is the most abundant species of the genus in Central Amazon. Its oleoresin has high ecological and socioeconomic importance for traditional peoples, standing out as one of the main Non-Timber Forest Products (NTFPs) traded in the region. However, access to the genetic diversity of C. multijuga is hampered by the absence of microsatellite markers specific for the species. In this sense, this study aimed to evaluate the transferability of eight microsatellite loci from copaiba-do-cerrado (Copaifera langsdorffii Desf.) to C. multijuga. Twenty-seven trees were sampled from a natural population in the Reserva Florestal Adolpho Ducke, Manaus, Amazonas, Brazil. DNA extraction was performed from approximately 120 mg of leaves, according to the CTAB (Cetyltrimethylammonium bromide) method of plant tissue extraction, with adaptations. The amplification protocol used was adapted from that proposed for C. langsdorffii. Six microsatellite loci were successfully amplified and characterized for C. multijuga. Genotype analysis revealed a total of 39 alleles, with an average of 6.5 per locus. The average expected heterozygosity was 0.692. The Polymorphism Information Content Index (PIC) revealed relevant levels for all loci used (> 50%), except for CL02 (48%). Only the CL01 locus showed a low level of heterozygosity. These markers, characterized for the first time for C. multijuga, represent a valuable tool for management strategies and future studies of mating system and population genetics of this species. |
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Transferability of microsatellite markers to study the genetic diversity of the copaibaheterologous amplifications, Copaifera multijuga Hayne, polymorphismmicrosatellite markersThe copaiba (Copaifera multijuga Hayne) is the most abundant species of the genus in Central Amazon. Its oleoresin has high ecological and socioeconomic importance for traditional peoples, standing out as one of the main Non-Timber Forest Products (NTFPs) traded in the region. However, access to the genetic diversity of C. multijuga is hampered by the absence of microsatellite markers specific for the species. In this sense, this study aimed to evaluate the transferability of eight microsatellite loci from copaiba-do-cerrado (Copaifera langsdorffii Desf.) to C. multijuga. Twenty-seven trees were sampled from a natural population in the Reserva Florestal Adolpho Ducke, Manaus, Amazonas, Brazil. DNA extraction was performed from approximately 120 mg of leaves, according to the CTAB (Cetyltrimethylammonium bromide) method of plant tissue extraction, with adaptations. The amplification protocol used was adapted from that proposed for C. langsdorffii. Six microsatellite loci were successfully amplified and characterized for C. multijuga. Genotype analysis revealed a total of 39 alleles, with an average of 6.5 per locus. The average expected heterozygosity was 0.692. The Polymorphism Information Content Index (PIC) revealed relevant levels for all loci used (> 50%), except for CL02 (48%). Only the CL01 locus showed a low level of heterozygosity. These markers, characterized for the first time for C. multijuga, represent a valuable tool for management strategies and future studies of mating system and population genetics of this species.A copaíba (Copaifera multijuga Hayne) é a espécie do gênero mais abundante na Amazônia Central. Seu oleorresina possui elevada importância ecológica e socioeconômica para os povos tradicionais, destacando-se como um dos um dos principais Produtos Florestais Não Madeireiros (PFNM) comercializados na região. Contudo, o acesso à diversidade genética de C. multijuga é dificultado pela ausência de marcadores microssatélites específicos para a espécie. Neste sentido, este estudo teve como objetivo avaliar a transferibilidade de oito locos microssatélites da copaíba-do-cerrado (Copaifera langsdorffii Desf.) para C. multijuga. Foram amostradas 27 árvores em população natural na Reserva Florestal Adolpho Ducke, Manaus, Amazonas. A extração de DNA foi realizada de aproximadamente 120 mg de folhas, conforme método de extração de tecidos vegetais CTAB (Brometo de Cetiltrimetilamônio), com adaptações. O protocolo de amplificação utilizado foi adaptado do proposto para C. langsdorffii. Seis locos microssatélites foram amplificados e caracterizados com sucesso para C. multijuga. A análise dos genótipos revelou um total de 39 alelos, com média de 6,5 por loco. A heterozigosidade esperada média foi de 0,692. O índice de Conteúdo Informativo de Polimorfismo (PIC) revelou níveis relevantes para todos os locos utilizados (> 50%), exceto para o CL02 (48%). Apenas o loco CL01 apresentou baixo nível de heterozigotos. Esses marcadores, caracterizados pela primeira vez para C. multijuga, representam uma ferramenta valiosa para estratégias de manejo e estudos futuros de sistema reprodutivo e genética populacional desta espécie.Universidade Federal Rural da Amazônia/UFRA2024-01-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/otherapplication/pdfhttps://ajaes.ufra.edu.br/index.php/ajaes/article/view/3611Amazonian Journal of Agricultural Sciences Journal of Agricultural and Environmental Sciences; Vol 66 (2023): RCA AJAES; 1-8Revista de Ciências Agrárias Amazonian Journal of Agricultural and Environmental Sciences; v. 66 (2023): RCA AJAES; 1-82177-87601517-591Xreponame:Revista de Ciências Agrárias (Belém. Online)instname:Universidade Federal Rural da Amazônia (UFRA)instacron:UFRAporhttps://ajaes.ufra.edu.br/index.php/ajaes/article/view/3611/1689Copyright (c) 2024 Moisés Teixeira Rolim Rolim, Rayssa Gomes Vasconcelos Vasconcelos , Antonio Saulo Cunha-Machado, Jacqueline da Silva Batista Batista, Paulo de Tarso Barbosa Sampaio Sampaio , Raquel da Silva Medeiros Medeiroshttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessRolim, Moisés Teixeira Rolim Vasconcelos , Rayssa Gomes Vasconcelos Cunha-Machado, Antonio SauloBatista, Jacqueline da Silva BatistaSampaio , Paulo de Tarso Barbosa Sampaio Medeiros, Raquel da Silva Medeiros 2024-01-22T19:48:40Zoai:ojs.www.periodicos.ufra.edu.br:article/3611Revistahttps://ajaes.ufra.edu.br/index.php/ajaes/PUBhttps://ajaes.ufra.edu.br/index.php/ajaes/oaiallan.lobato@ufra.edu.br || ajaes.suporte@gmail.com2177-87601517-591Xopendoar:2024-01-22T19:48:40Revista de Ciências Agrárias (Belém. Online) - Universidade Federal Rural da Amazônia (UFRA)false |
dc.title.none.fl_str_mv |
Transferability of microsatellite markers to study the genetic diversity of the copaiba |
title |
Transferability of microsatellite markers to study the genetic diversity of the copaiba |
spellingShingle |
Transferability of microsatellite markers to study the genetic diversity of the copaiba Rolim, Moisés Teixeira Rolim heterologous amplifications, Copaifera multijuga Hayne, polymorphism microsatellite markers |
title_short |
Transferability of microsatellite markers to study the genetic diversity of the copaiba |
title_full |
Transferability of microsatellite markers to study the genetic diversity of the copaiba |
title_fullStr |
Transferability of microsatellite markers to study the genetic diversity of the copaiba |
title_full_unstemmed |
Transferability of microsatellite markers to study the genetic diversity of the copaiba |
title_sort |
Transferability of microsatellite markers to study the genetic diversity of the copaiba |
author |
Rolim, Moisés Teixeira Rolim |
author_facet |
Rolim, Moisés Teixeira Rolim Vasconcelos , Rayssa Gomes Vasconcelos Cunha-Machado, Antonio Saulo Batista, Jacqueline da Silva Batista Sampaio , Paulo de Tarso Barbosa Sampaio Medeiros, Raquel da Silva Medeiros |
author_role |
author |
author2 |
Vasconcelos , Rayssa Gomes Vasconcelos Cunha-Machado, Antonio Saulo Batista, Jacqueline da Silva Batista Sampaio , Paulo de Tarso Barbosa Sampaio Medeiros, Raquel da Silva Medeiros |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rolim, Moisés Teixeira Rolim Vasconcelos , Rayssa Gomes Vasconcelos Cunha-Machado, Antonio Saulo Batista, Jacqueline da Silva Batista Sampaio , Paulo de Tarso Barbosa Sampaio Medeiros, Raquel da Silva Medeiros |
dc.subject.por.fl_str_mv |
heterologous amplifications, Copaifera multijuga Hayne, polymorphism microsatellite markers |
topic |
heterologous amplifications, Copaifera multijuga Hayne, polymorphism microsatellite markers |
description |
The copaiba (Copaifera multijuga Hayne) is the most abundant species of the genus in Central Amazon. Its oleoresin has high ecological and socioeconomic importance for traditional peoples, standing out as one of the main Non-Timber Forest Products (NTFPs) traded in the region. However, access to the genetic diversity of C. multijuga is hampered by the absence of microsatellite markers specific for the species. In this sense, this study aimed to evaluate the transferability of eight microsatellite loci from copaiba-do-cerrado (Copaifera langsdorffii Desf.) to C. multijuga. Twenty-seven trees were sampled from a natural population in the Reserva Florestal Adolpho Ducke, Manaus, Amazonas, Brazil. DNA extraction was performed from approximately 120 mg of leaves, according to the CTAB (Cetyltrimethylammonium bromide) method of plant tissue extraction, with adaptations. The amplification protocol used was adapted from that proposed for C. langsdorffii. Six microsatellite loci were successfully amplified and characterized for C. multijuga. Genotype analysis revealed a total of 39 alleles, with an average of 6.5 per locus. The average expected heterozygosity was 0.692. The Polymorphism Information Content Index (PIC) revealed relevant levels for all loci used (> 50%), except for CL02 (48%). Only the CL01 locus showed a low level of heterozygosity. These markers, characterized for the first time for C. multijuga, represent a valuable tool for management strategies and future studies of mating system and population genetics of this species. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-01-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/other |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ajaes.ufra.edu.br/index.php/ajaes/article/view/3611 |
url |
https://ajaes.ufra.edu.br/index.php/ajaes/article/view/3611 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ajaes.ufra.edu.br/index.php/ajaes/article/view/3611/1689 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural da Amazônia/UFRA |
publisher.none.fl_str_mv |
Universidade Federal Rural da Amazônia/UFRA |
dc.source.none.fl_str_mv |
Amazonian Journal of Agricultural Sciences Journal of Agricultural and Environmental Sciences; Vol 66 (2023): RCA AJAES; 1-8 Revista de Ciências Agrárias Amazonian Journal of Agricultural and Environmental Sciences; v. 66 (2023): RCA AJAES; 1-8 2177-8760 1517-591X reponame:Revista de Ciências Agrárias (Belém. Online) instname:Universidade Federal Rural da Amazônia (UFRA) instacron:UFRA |
instname_str |
Universidade Federal Rural da Amazônia (UFRA) |
instacron_str |
UFRA |
institution |
UFRA |
reponame_str |
Revista de Ciências Agrárias (Belém. Online) |
collection |
Revista de Ciências Agrárias (Belém. Online) |
repository.name.fl_str_mv |
Revista de Ciências Agrárias (Belém. Online) - Universidade Federal Rural da Amazônia (UFRA) |
repository.mail.fl_str_mv |
allan.lobato@ufra.edu.br || ajaes.suporte@gmail.com |
_version_ |
1797231625917956096 |