Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.biopha.2017.11.099 http://hdl.handle.net/11449/159979 |
Resumo: | Copaiba oil is widely used in medicine, but there are no reports regarding its application in ophthalmology. Therefore, the objective of this study was to evaluate the clinical, histopathological and toxicogenetic effects of eye drops containing 0.1 and 0.5% of Copaifera multijuga Hayne oil on superficial corneal ulcers induced with alkali in the left eye of rats. For histological analysis, the percent reduction in ulcers and thickness of the corneal epithelium and stroma were evaluated 48 and 72 h after ulcer induction. Additionally, neovascularization and polymorphonuclear infiltration were classified in the stroma. The bone marrow micronucleus test was used for toxicogenetic assessment. None of the animals exhibited clinical signs of immediate ocular discomfort after instillation and the eye drops were harmless to the ocular surface. There was a significant difference in percent ulcer reduction and corneal stroma thickness between animals treated with the C. multijuga eye drops and untreated animals with corneal injury and the negative control, respectively, suggesting a healing effect of the oleoresin. Analysis of the thickness of the corneal epithelium at the two time points showed that the eye drops formulated did not significantly reduce the damage caused by alkali. The same was observed for the treatments with the reference drugs. No difference in stromal neovascularization or inflammatory infiltration was observed between the treated groups. The toxicogenetic results revealed the absence of cytotoxicity and genotoxicity of the treatments. In conclusion, the C. multijuga eye drops did not cause damage to the ocular surface under the present experimental conditions and corneal epithelization was similar to the conventional treatments. These results indicate that eye drops containing C. multijuga oleoresin are a promising option for the treatment of superficial keratitis. |
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Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic studyCopaifera multijugaHayneUlcerative keratitisCorneal re-epithelizationGenotoxicityCopaiba oil is widely used in medicine, but there are no reports regarding its application in ophthalmology. Therefore, the objective of this study was to evaluate the clinical, histopathological and toxicogenetic effects of eye drops containing 0.1 and 0.5% of Copaifera multijuga Hayne oil on superficial corneal ulcers induced with alkali in the left eye of rats. For histological analysis, the percent reduction in ulcers and thickness of the corneal epithelium and stroma were evaluated 48 and 72 h after ulcer induction. Additionally, neovascularization and polymorphonuclear infiltration were classified in the stroma. The bone marrow micronucleus test was used for toxicogenetic assessment. None of the animals exhibited clinical signs of immediate ocular discomfort after instillation and the eye drops were harmless to the ocular surface. There was a significant difference in percent ulcer reduction and corneal stroma thickness between animals treated with the C. multijuga eye drops and untreated animals with corneal injury and the negative control, respectively, suggesting a healing effect of the oleoresin. Analysis of the thickness of the corneal epithelium at the two time points showed that the eye drops formulated did not significantly reduce the damage caused by alkali. The same was observed for the treatments with the reference drugs. No difference in stromal neovascularization or inflammatory infiltration was observed between the treated groups. The toxicogenetic results revealed the absence of cytotoxicity and genotoxicity of the treatments. In conclusion, the C. multijuga eye drops did not cause damage to the ocular surface under the present experimental conditions and corneal epithelization was similar to the conventional treatments. These results indicate that eye drops containing C. multijuga oleoresin are a promising option for the treatment of superficial keratitis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Franca, Ave Dr Armando Salles de Oliveira 201, BR-14404600 Franca, SP, BrazilUniv Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP, BrazilSao Paulo State Univ, Via Acesso Prof Paulo Donato Castelane S-N, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ, Via Acesso Prof Paulo Donato Castelane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2011/13630-7Elsevier B.V.Univ FrancaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Goncalves Dias, Fernanda GosuenJorge, Adriana TorrecilhasPereira, Lucas de FreitasFurtado, Ricardo AndradeAmbrosio, Sergio RicardoBastos, Jairo KenuppRamos, Salvador BoccalettiChahud, FernandoGosuen Goncalves Dias, Luis Gustavo [UNESP]Honsho, Cristiane dos SantosTavares, Denise Crispim2018-11-26T15:45:59Z2018-11-26T15:45:59Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1193-1198application/pdfhttp://dx.doi.org/10.1016/j.biopha.2017.11.099Biomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 96, p. 1193-1198, 2017.0753-3322http://hdl.handle.net/11449/15997910.1016/j.biopha.2017.11.099WOS:000418502100142WOS000418502100142.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomedicine & Pharmacotherapy0,951info:eu-repo/semantics/openAccess2023-11-21T06:15:42Zoai:repositorio.unesp.br:11449/159979Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:23:04.348795Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study |
title |
Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study |
spellingShingle |
Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study Goncalves Dias, Fernanda Gosuen Copaifera multijuga Hayne Ulcerative keratitis Corneal re-epithelization Genotoxicity |
title_short |
Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study |
title_full |
Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study |
title_fullStr |
Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study |
title_full_unstemmed |
Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study |
title_sort |
Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study |
author |
Goncalves Dias, Fernanda Gosuen |
author_facet |
Goncalves Dias, Fernanda Gosuen Jorge, Adriana Torrecilhas Pereira, Lucas de Freitas Furtado, Ricardo Andrade Ambrosio, Sergio Ricardo Bastos, Jairo Kenupp Ramos, Salvador Boccaletti Chahud, Fernando Gosuen Goncalves Dias, Luis Gustavo [UNESP] Honsho, Cristiane dos Santos Tavares, Denise Crispim |
author_role |
author |
author2 |
Jorge, Adriana Torrecilhas Pereira, Lucas de Freitas Furtado, Ricardo Andrade Ambrosio, Sergio Ricardo Bastos, Jairo Kenupp Ramos, Salvador Boccaletti Chahud, Fernando Gosuen Goncalves Dias, Luis Gustavo [UNESP] Honsho, Cristiane dos Santos Tavares, Denise Crispim |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Franca Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Goncalves Dias, Fernanda Gosuen Jorge, Adriana Torrecilhas Pereira, Lucas de Freitas Furtado, Ricardo Andrade Ambrosio, Sergio Ricardo Bastos, Jairo Kenupp Ramos, Salvador Boccaletti Chahud, Fernando Gosuen Goncalves Dias, Luis Gustavo [UNESP] Honsho, Cristiane dos Santos Tavares, Denise Crispim |
dc.subject.por.fl_str_mv |
Copaifera multijuga Hayne Ulcerative keratitis Corneal re-epithelization Genotoxicity |
topic |
Copaifera multijuga Hayne Ulcerative keratitis Corneal re-epithelization Genotoxicity |
description |
Copaiba oil is widely used in medicine, but there are no reports regarding its application in ophthalmology. Therefore, the objective of this study was to evaluate the clinical, histopathological and toxicogenetic effects of eye drops containing 0.1 and 0.5% of Copaifera multijuga Hayne oil on superficial corneal ulcers induced with alkali in the left eye of rats. For histological analysis, the percent reduction in ulcers and thickness of the corneal epithelium and stroma were evaluated 48 and 72 h after ulcer induction. Additionally, neovascularization and polymorphonuclear infiltration were classified in the stroma. The bone marrow micronucleus test was used for toxicogenetic assessment. None of the animals exhibited clinical signs of immediate ocular discomfort after instillation and the eye drops were harmless to the ocular surface. There was a significant difference in percent ulcer reduction and corneal stroma thickness between animals treated with the C. multijuga eye drops and untreated animals with corneal injury and the negative control, respectively, suggesting a healing effect of the oleoresin. Analysis of the thickness of the corneal epithelium at the two time points showed that the eye drops formulated did not significantly reduce the damage caused by alkali. The same was observed for the treatments with the reference drugs. No difference in stromal neovascularization or inflammatory infiltration was observed between the treated groups. The toxicogenetic results revealed the absence of cytotoxicity and genotoxicity of the treatments. In conclusion, the C. multijuga eye drops did not cause damage to the ocular surface under the present experimental conditions and corneal epithelization was similar to the conventional treatments. These results indicate that eye drops containing C. multijuga oleoresin are a promising option for the treatment of superficial keratitis. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 2018-11-26T15:45:59Z 2018-11-26T15:45:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.biopha.2017.11.099 Biomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 96, p. 1193-1198, 2017. 0753-3322 http://hdl.handle.net/11449/159979 10.1016/j.biopha.2017.11.099 WOS:000418502100142 WOS000418502100142.pdf |
url |
http://dx.doi.org/10.1016/j.biopha.2017.11.099 http://hdl.handle.net/11449/159979 |
identifier_str_mv |
Biomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 96, p. 1193-1198, 2017. 0753-3322 10.1016/j.biopha.2017.11.099 WOS:000418502100142 WOS000418502100142.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomedicine & Pharmacotherapy 0,951 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1193-1198 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128925314842624 |