Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study

Detalhes bibliográficos
Autor(a) principal: Goncalves Dias, Fernanda Gosuen
Data de Publicação: 2017
Outros Autores: Jorge, Adriana Torrecilhas, Pereira, Lucas de Freitas, Furtado, Ricardo Andrade, Ambrosio, Sergio Ricardo, Bastos, Jairo Kenupp, Ramos, Salvador Boccaletti, Chahud, Fernando, Gosuen Goncalves Dias, Luis Gustavo [UNESP], Honsho, Cristiane dos Santos, Tavares, Denise Crispim
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.biopha.2017.11.099
http://hdl.handle.net/11449/159979
Resumo: Copaiba oil is widely used in medicine, but there are no reports regarding its application in ophthalmology. Therefore, the objective of this study was to evaluate the clinical, histopathological and toxicogenetic effects of eye drops containing 0.1 and 0.5% of Copaifera multijuga Hayne oil on superficial corneal ulcers induced with alkali in the left eye of rats. For histological analysis, the percent reduction in ulcers and thickness of the corneal epithelium and stroma were evaluated 48 and 72 h after ulcer induction. Additionally, neovascularization and polymorphonuclear infiltration were classified in the stroma. The bone marrow micronucleus test was used for toxicogenetic assessment. None of the animals exhibited clinical signs of immediate ocular discomfort after instillation and the eye drops were harmless to the ocular surface. There was a significant difference in percent ulcer reduction and corneal stroma thickness between animals treated with the C. multijuga eye drops and untreated animals with corneal injury and the negative control, respectively, suggesting a healing effect of the oleoresin. Analysis of the thickness of the corneal epithelium at the two time points showed that the eye drops formulated did not significantly reduce the damage caused by alkali. The same was observed for the treatments with the reference drugs. No difference in stromal neovascularization or inflammatory infiltration was observed between the treated groups. The toxicogenetic results revealed the absence of cytotoxicity and genotoxicity of the treatments. In conclusion, the C. multijuga eye drops did not cause damage to the ocular surface under the present experimental conditions and corneal epithelization was similar to the conventional treatments. These results indicate that eye drops containing C. multijuga oleoresin are a promising option for the treatment of superficial keratitis.
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spelling Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic studyCopaifera multijugaHayneUlcerative keratitisCorneal re-epithelizationGenotoxicityCopaiba oil is widely used in medicine, but there are no reports regarding its application in ophthalmology. Therefore, the objective of this study was to evaluate the clinical, histopathological and toxicogenetic effects of eye drops containing 0.1 and 0.5% of Copaifera multijuga Hayne oil on superficial corneal ulcers induced with alkali in the left eye of rats. For histological analysis, the percent reduction in ulcers and thickness of the corneal epithelium and stroma were evaluated 48 and 72 h after ulcer induction. Additionally, neovascularization and polymorphonuclear infiltration were classified in the stroma. The bone marrow micronucleus test was used for toxicogenetic assessment. None of the animals exhibited clinical signs of immediate ocular discomfort after instillation and the eye drops were harmless to the ocular surface. There was a significant difference in percent ulcer reduction and corneal stroma thickness between animals treated with the C. multijuga eye drops and untreated animals with corneal injury and the negative control, respectively, suggesting a healing effect of the oleoresin. Analysis of the thickness of the corneal epithelium at the two time points showed that the eye drops formulated did not significantly reduce the damage caused by alkali. The same was observed for the treatments with the reference drugs. No difference in stromal neovascularization or inflammatory infiltration was observed between the treated groups. The toxicogenetic results revealed the absence of cytotoxicity and genotoxicity of the treatments. In conclusion, the C. multijuga eye drops did not cause damage to the ocular surface under the present experimental conditions and corneal epithelization was similar to the conventional treatments. These results indicate that eye drops containing C. multijuga oleoresin are a promising option for the treatment of superficial keratitis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Franca, Ave Dr Armando Salles de Oliveira 201, BR-14404600 Franca, SP, BrazilUniv Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP, BrazilSao Paulo State Univ, Via Acesso Prof Paulo Donato Castelane S-N, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ, Via Acesso Prof Paulo Donato Castelane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2011/13630-7Elsevier B.V.Univ FrancaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Goncalves Dias, Fernanda GosuenJorge, Adriana TorrecilhasPereira, Lucas de FreitasFurtado, Ricardo AndradeAmbrosio, Sergio RicardoBastos, Jairo KenuppRamos, Salvador BoccalettiChahud, FernandoGosuen Goncalves Dias, Luis Gustavo [UNESP]Honsho, Cristiane dos SantosTavares, Denise Crispim2018-11-26T15:45:59Z2018-11-26T15:45:59Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1193-1198application/pdfhttp://dx.doi.org/10.1016/j.biopha.2017.11.099Biomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 96, p. 1193-1198, 2017.0753-3322http://hdl.handle.net/11449/15997910.1016/j.biopha.2017.11.099WOS:000418502100142WOS000418502100142.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomedicine & Pharmacotherapy0,951info:eu-repo/semantics/openAccess2023-11-21T06:15:42Zoai:repositorio.unesp.br:11449/159979Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:23:04.348795Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study
title Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study
spellingShingle Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study
Goncalves Dias, Fernanda Gosuen
Copaifera multijuga
Hayne
Ulcerative keratitis
Corneal re-epithelization
Genotoxicity
title_short Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study
title_full Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study
title_fullStr Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study
title_full_unstemmed Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study
title_sort Use of Copaifera multijuga for acute corneal repair after chemical injury: A clinical, histopathological and toxicogenetic study
author Goncalves Dias, Fernanda Gosuen
author_facet Goncalves Dias, Fernanda Gosuen
Jorge, Adriana Torrecilhas
Pereira, Lucas de Freitas
Furtado, Ricardo Andrade
Ambrosio, Sergio Ricardo
Bastos, Jairo Kenupp
Ramos, Salvador Boccaletti
Chahud, Fernando
Gosuen Goncalves Dias, Luis Gustavo [UNESP]
Honsho, Cristiane dos Santos
Tavares, Denise Crispim
author_role author
author2 Jorge, Adriana Torrecilhas
Pereira, Lucas de Freitas
Furtado, Ricardo Andrade
Ambrosio, Sergio Ricardo
Bastos, Jairo Kenupp
Ramos, Salvador Boccaletti
Chahud, Fernando
Gosuen Goncalves Dias, Luis Gustavo [UNESP]
Honsho, Cristiane dos Santos
Tavares, Denise Crispim
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Franca
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Goncalves Dias, Fernanda Gosuen
Jorge, Adriana Torrecilhas
Pereira, Lucas de Freitas
Furtado, Ricardo Andrade
Ambrosio, Sergio Ricardo
Bastos, Jairo Kenupp
Ramos, Salvador Boccaletti
Chahud, Fernando
Gosuen Goncalves Dias, Luis Gustavo [UNESP]
Honsho, Cristiane dos Santos
Tavares, Denise Crispim
dc.subject.por.fl_str_mv Copaifera multijuga
Hayne
Ulcerative keratitis
Corneal re-epithelization
Genotoxicity
topic Copaifera multijuga
Hayne
Ulcerative keratitis
Corneal re-epithelization
Genotoxicity
description Copaiba oil is widely used in medicine, but there are no reports regarding its application in ophthalmology. Therefore, the objective of this study was to evaluate the clinical, histopathological and toxicogenetic effects of eye drops containing 0.1 and 0.5% of Copaifera multijuga Hayne oil on superficial corneal ulcers induced with alkali in the left eye of rats. For histological analysis, the percent reduction in ulcers and thickness of the corneal epithelium and stroma were evaluated 48 and 72 h after ulcer induction. Additionally, neovascularization and polymorphonuclear infiltration were classified in the stroma. The bone marrow micronucleus test was used for toxicogenetic assessment. None of the animals exhibited clinical signs of immediate ocular discomfort after instillation and the eye drops were harmless to the ocular surface. There was a significant difference in percent ulcer reduction and corneal stroma thickness between animals treated with the C. multijuga eye drops and untreated animals with corneal injury and the negative control, respectively, suggesting a healing effect of the oleoresin. Analysis of the thickness of the corneal epithelium at the two time points showed that the eye drops formulated did not significantly reduce the damage caused by alkali. The same was observed for the treatments with the reference drugs. No difference in stromal neovascularization or inflammatory infiltration was observed between the treated groups. The toxicogenetic results revealed the absence of cytotoxicity and genotoxicity of the treatments. In conclusion, the C. multijuga eye drops did not cause damage to the ocular surface under the present experimental conditions and corneal epithelization was similar to the conventional treatments. These results indicate that eye drops containing C. multijuga oleoresin are a promising option for the treatment of superficial keratitis.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
2018-11-26T15:45:59Z
2018-11-26T15:45:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.biopha.2017.11.099
Biomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 96, p. 1193-1198, 2017.
0753-3322
http://hdl.handle.net/11449/159979
10.1016/j.biopha.2017.11.099
WOS:000418502100142
WOS000418502100142.pdf
url http://dx.doi.org/10.1016/j.biopha.2017.11.099
http://hdl.handle.net/11449/159979
identifier_str_mv Biomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 96, p. 1193-1198, 2017.
0753-3322
10.1016/j.biopha.2017.11.099
WOS:000418502100142
WOS000418502100142.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biomedicine & Pharmacotherapy
0,951
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1193-1198
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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