Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested

Detalhes bibliográficos
Autor(a) principal: Coser, Tiago Bottin
Data de Publicação: 2008
Outros Autores: Chesky, Marisa, de-Paris, Fernanda, Barth, Afonso Luis, Schmitt, Virginia Minghelli, Pinheiro Machado, Alice Beatriz Mombach
Tipo de documento: Artigo
Idioma: por
Título da fonte: Clinical and Biomedical Research
Texto Completo: https://seer.ufrgs.br/index.php/hcpa/article/view/2945
Resumo: All over the world, the hepatitis B virus (HBV) is considered one of the major problems of public health, despite vaccination. World Health Organization (WHO) estimates that more than 2 billions of persons are infected by HBV. Brazil is classified as an area of intermediary incidence by WHO. However, prevalence studies have detected differences of infection indexes in geographic re-gions: 8% in the Amazonian region, 2,5% in middle-west and Northeast, 2% in Southeast and 1% in South. A sensitive and specific diagnosis is very important to the HBV carrier patients. The aim of this study was to determine the minimum limit of detection of the nested PCR in house technique for HBV. Serial dilutions of one quantified sample of HBV (1000 copies/mL; 750 copies/mL; 500 copies/mL; 250 copies/mL) were submitted to a nested PCR. The target of PCR was viral core and pre-core region. Commercial kit, QiAmp, was employed to purify nucleic acids from the sample. The minimum detection limit found was 500 copies/mL or 10 copies per PCR reaction.
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spelling Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nestedDeterminação do Limite Mínimo de Detecção da Técnica de PCR “Nested” para o Vírus da Hepatite B (HBV)HBVPCRHepatite BHepatite BAll over the world, the hepatitis B virus (HBV) is considered one of the major problems of public health, despite vaccination. World Health Organization (WHO) estimates that more than 2 billions of persons are infected by HBV. Brazil is classified as an area of intermediary incidence by WHO. However, prevalence studies have detected differences of infection indexes in geographic re-gions: 8% in the Amazonian region, 2,5% in middle-west and Northeast, 2% in Southeast and 1% in South. A sensitive and specific diagnosis is very important to the HBV carrier patients. The aim of this study was to determine the minimum limit of detection of the nested PCR in house technique for HBV. Serial dilutions of one quantified sample of HBV (1000 copies/mL; 750 copies/mL; 500 copies/mL; 250 copies/mL) were submitted to a nested PCR. The target of PCR was viral core and pre-core region. Commercial kit, QiAmp, was employed to purify nucleic acids from the sample. The minimum detection limit found was 500 copies/mL or 10 copies per PCR reaction.Mundialmente, a hepatite pelo vírus B (HBV) é considerada um dos maiores problemas de saúde pública, apesar da vacina-ção. A Organização Mundial da Saúde (OMS) estima que mais de 2 bilhões de pessoas estejam infectadas pelo HBV. O Brasil é classificado como área de incidência intermediária pela OMS. No entanto, estudos de prevalência detectaram diferenças de índices de infecção nas regiões geográficas: 8% na região Amazônica, 2,5% nas regiões Centro-Oeste e Nordeste, 2% na Sudeste e 1% na região Sul. Um diagnóstico sensível e específico é de fundamental importância para os pacientes portadores do HBV. O objetivo deste estudo foi determinar o limite mínimo de detecção da técnica de PCR “nested” “in house” para o HBV. Diluições seriadas de uma amostra quantificada de HBV (1000 cópias/mL; 750 cópias/mL; 500 cópias/mL; 250 cópias/mL) foram submetidas à técnica de PCR “nested”. O alvo da amplificação por PCR foi a região do core e pré-core do vírus. Para extração dos ácidos nucléicos da amostra foi empregado o kit comercial QIAmp. O limite mínimo de detecção encontrado foi de 500 cópias/mL ou 10 cópias por reação de PCR.HCPA/FAMED/UFRGS2008-06-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed ArticleAvaliado por Paresapplication/pdfhttps://seer.ufrgs.br/index.php/hcpa/article/view/2945Clinical & Biomedical Research; Vol. 28 No. 1 (2008): Revista HCPAClinical and Biomedical Research; v. 28 n. 1 (2008): Revista HCPA2357-9730reponame:Clinical and Biomedical Researchinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSporhttps://seer.ufrgs.br/index.php/hcpa/article/view/2945/2531Coser, Tiago BottinChesky, Marisade-Paris, FernandaBarth, Afonso LuisSchmitt, Virginia MinghelliPinheiro Machado, Alice Beatriz Mombachinfo:eu-repo/semantics/openAccess2020-01-16T16:15:43Zoai:seer.ufrgs.br:article/2945Revistahttps://www.seer.ufrgs.br/index.php/hcpaPUBhttps://seer.ufrgs.br/index.php/hcpa/oai||cbr@hcpa.edu.br2357-97302357-9730opendoar:2020-01-16T16:15:43Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.none.fl_str_mv Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested
Determinação do Limite Mínimo de Detecção da Técnica de PCR “Nested” para o Vírus da Hepatite B (HBV)
title Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested
spellingShingle Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested
Coser, Tiago Bottin
HBV
PCR
Hepatite B
Hepatite B
title_short Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested
title_full Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested
title_fullStr Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested
title_full_unstemmed Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested
title_sort Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested
author Coser, Tiago Bottin
author_facet Coser, Tiago Bottin
Chesky, Marisa
de-Paris, Fernanda
Barth, Afonso Luis
Schmitt, Virginia Minghelli
Pinheiro Machado, Alice Beatriz Mombach
author_role author
author2 Chesky, Marisa
de-Paris, Fernanda
Barth, Afonso Luis
Schmitt, Virginia Minghelli
Pinheiro Machado, Alice Beatriz Mombach
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Coser, Tiago Bottin
Chesky, Marisa
de-Paris, Fernanda
Barth, Afonso Luis
Schmitt, Virginia Minghelli
Pinheiro Machado, Alice Beatriz Mombach
dc.subject.por.fl_str_mv HBV
PCR
Hepatite B
Hepatite B
topic HBV
PCR
Hepatite B
Hepatite B
description All over the world, the hepatitis B virus (HBV) is considered one of the major problems of public health, despite vaccination. World Health Organization (WHO) estimates that more than 2 billions of persons are infected by HBV. Brazil is classified as an area of intermediary incidence by WHO. However, prevalence studies have detected differences of infection indexes in geographic re-gions: 8% in the Amazonian region, 2,5% in middle-west and Northeast, 2% in Southeast and 1% in South. A sensitive and specific diagnosis is very important to the HBV carrier patients. The aim of this study was to determine the minimum limit of detection of the nested PCR in house technique for HBV. Serial dilutions of one quantified sample of HBV (1000 copies/mL; 750 copies/mL; 500 copies/mL; 250 copies/mL) were submitted to a nested PCR. The target of PCR was viral core and pre-core region. Commercial kit, QiAmp, was employed to purify nucleic acids from the sample. The minimum detection limit found was 500 copies/mL or 10 copies per PCR reaction.
publishDate 2008
dc.date.none.fl_str_mv 2008-06-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
Avaliado por Pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufrgs.br/index.php/hcpa/article/view/2945
url https://seer.ufrgs.br/index.php/hcpa/article/view/2945
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.ufrgs.br/index.php/hcpa/article/view/2945/2531
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv HCPA/FAMED/UFRGS
publisher.none.fl_str_mv HCPA/FAMED/UFRGS
dc.source.none.fl_str_mv Clinical & Biomedical Research; Vol. 28 No. 1 (2008): Revista HCPA
Clinical and Biomedical Research; v. 28 n. 1 (2008): Revista HCPA
2357-9730
reponame:Clinical and Biomedical Research
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Clinical and Biomedical Research
collection Clinical and Biomedical Research
repository.name.fl_str_mv Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)
repository.mail.fl_str_mv ||cbr@hcpa.edu.br
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