Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test

Detalhes bibliográficos
Autor(a) principal: Rozales, Franciéli Pedrotti
Data de Publicação: 2014
Outros Autores: de-Paris, Fernada, Machado, Alice Beatriz Mombach Pinheiro, Costi, Cintia, Barth, Afonso Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinical and Biomedical Research
Texto Completo: https://seer.ufrgs.br/index.php/hcpa/article/view/46502
Resumo: Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure. 
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spelling Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic testHCVDiagnostic testX-tailAcute diseaseInfection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure. HCPA/FAMED/UFRGS2014-07-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed ArticleAvaliado por Paresapplication/pdfhttps://seer.ufrgs.br/index.php/hcpa/article/view/46502Clinical & Biomedical Research; Vol. 34 No. 2 (2014): Clinical and Biomedical ResearchClinical and Biomedical Research; v. 34 n. 2 (2014): Clinical and Biomedical Research2357-9730reponame:Clinical and Biomedical Researchinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSenghttps://seer.ufrgs.br/index.php/hcpa/article/view/46502/30860Rozales, Franciéli Pedrottide-Paris, FernadaMachado, Alice Beatriz Mombach PinheiroCosti, CintiaBarth, Afonso Luisinfo:eu-repo/semantics/openAccess2024-01-19T13:30:44Zoai:seer.ufrgs.br:article/46502Revistahttps://www.seer.ufrgs.br/index.php/hcpaPUBhttps://seer.ufrgs.br/index.php/hcpa/oai||cbr@hcpa.edu.br2357-97302357-9730opendoar:2024-01-19T13:30:44Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.none.fl_str_mv Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
title Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
spellingShingle Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
Rozales, Franciéli Pedrotti
HCV
Diagnostic test
X-tail
Acute disease
title_short Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
title_full Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
title_fullStr Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
title_full_unstemmed Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
title_sort Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
author Rozales, Franciéli Pedrotti
author_facet Rozales, Franciéli Pedrotti
de-Paris, Fernada
Machado, Alice Beatriz Mombach Pinheiro
Costi, Cintia
Barth, Afonso Luis
author_role author
author2 de-Paris, Fernada
Machado, Alice Beatriz Mombach Pinheiro
Costi, Cintia
Barth, Afonso Luis
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rozales, Franciéli Pedrotti
de-Paris, Fernada
Machado, Alice Beatriz Mombach Pinheiro
Costi, Cintia
Barth, Afonso Luis
dc.subject.por.fl_str_mv HCV
Diagnostic test
X-tail
Acute disease
topic HCV
Diagnostic test
X-tail
Acute disease
description Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure. 
publishDate 2014
dc.date.none.fl_str_mv 2014-07-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
Avaliado por Pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufrgs.br/index.php/hcpa/article/view/46502
url https://seer.ufrgs.br/index.php/hcpa/article/view/46502
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufrgs.br/index.php/hcpa/article/view/46502/30860
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv HCPA/FAMED/UFRGS
publisher.none.fl_str_mv HCPA/FAMED/UFRGS
dc.source.none.fl_str_mv Clinical & Biomedical Research; Vol. 34 No. 2 (2014): Clinical and Biomedical Research
Clinical and Biomedical Research; v. 34 n. 2 (2014): Clinical and Biomedical Research
2357-9730
reponame:Clinical and Biomedical Research
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Clinical and Biomedical Research
collection Clinical and Biomedical Research
repository.name.fl_str_mv Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)
repository.mail.fl_str_mv ||cbr@hcpa.edu.br
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