A3 receptor agonist modulates IL-1β hippocampus levels in a rat model of neuropathic pain
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Clinical and Biomedical Research |
Texto Completo: | https://seer.ufrgs.br/index.php/hcpa/article/view/116706 |
Resumo: | Introduction: Considering the lack of specific treatments to neuropathic pain, this study aimed to evaluate the effect of a single dose adenosine A3 receptor IB-MECA in the inflammatory and neurotrophic parameters of rats submitted to a neuropathic pain model. Methods: 64 adults male Wistar rats were used. Neuropathic pain was induced by the chronic constriction injury (CCI) of sciatic nerve and the treatment consisted in one dose of 0.5 μmol/kg of a selective agonist of adenosine A3 receptor IB-MECA dissolved in 3% DMSO; vehicle groups received DMSO 3% in saline; morphine groups received 5mg/kg Cerebral cortex and hippocampus IL-1β, BDNF and NGF levels were determined by ELISA assay. Results: The key finding was that a single dose of IB-MECA was able to modulate the IL-1β hippocampus levels CCI and the DMSO increased IL-1β and NGF hippocampus levels in sham animals; however, when the DMSO as an IB-MECA vehicle, this effect was not observed, indicating that IB-MECA was able to prevent the effect of DMSO. Conclusions: Considering that the IL-1β role in neuropathic pain is quite explored, as well as the hippocampus contributions, our result corroborates the relationship of A3 receptor and the chronic pain maintenance process. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0in; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman";}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes;}div.WordSection1 {page:WordSection1;} |
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oai:seer.ufrgs.br:article/116706 |
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UFRGS-20 |
network_name_str |
Clinical and Biomedical Research |
repository_id_str |
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spelling |
A3 receptor agonist modulates IL-1β hippocampus levels in a rat model of neuropathic painadenosine A3 receptorcytokineDMSOIB-MECAneuropathic painneurotrophinrats.Introduction: Considering the lack of specific treatments to neuropathic pain, this study aimed to evaluate the effect of a single dose adenosine A3 receptor IB-MECA in the inflammatory and neurotrophic parameters of rats submitted to a neuropathic pain model. Methods: 64 adults male Wistar rats were used. Neuropathic pain was induced by the chronic constriction injury (CCI) of sciatic nerve and the treatment consisted in one dose of 0.5 μmol/kg of a selective agonist of adenosine A3 receptor IB-MECA dissolved in 3% DMSO; vehicle groups received DMSO 3% in saline; morphine groups received 5mg/kg Cerebral cortex and hippocampus IL-1β, BDNF and NGF levels were determined by ELISA assay. Results: The key finding was that a single dose of IB-MECA was able to modulate the IL-1β hippocampus levels CCI and the DMSO increased IL-1β and NGF hippocampus levels in sham animals; however, when the DMSO as an IB-MECA vehicle, this effect was not observed, indicating that IB-MECA was able to prevent the effect of DMSO. Conclusions: Considering that the IL-1β role in neuropathic pain is quite explored, as well as the hippocampus contributions, our result corroborates the relationship of A3 receptor and the chronic pain maintenance process. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0in; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman";}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes;}div.WordSection1 {page:WordSection1;}HCPA/FAMED/UFRGS2022-07-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed ArticleAvaliado por Paresapplication/pdfhttps://seer.ufrgs.br/index.php/hcpa/article/view/116706Clinical & Biomedical Research; Vol. 42 No. 2 (2022): Clinical & Biomedical ResearchClinical and Biomedical Research; v. 42 n. 2 (2022): Clinical and Biomedical Research2357-9730reponame:Clinical and Biomedical Researchinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSporhttps://seer.ufrgs.br/index.php/hcpa/article/view/116706/85500Copyright (c) 2022 Clinical and Biomedical Researchhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCioato, Stefania GiottiMedeiros, Liciane FernandesLopes, Bettega CostaMedeiros, Helouise RichardtCaumo, WolneiRoesler, RafaelTorres, Iraci LS2024-01-19T14:12:37Zoai:seer.ufrgs.br:article/116706Revistahttps://www.seer.ufrgs.br/index.php/hcpaPUBhttps://seer.ufrgs.br/index.php/hcpa/oai||cbr@hcpa.edu.br2357-97302357-9730opendoar:2024-01-19T14:12:37Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.none.fl_str_mv |
A3 receptor agonist modulates IL-1β hippocampus levels in a rat model of neuropathic pain |
title |
A3 receptor agonist modulates IL-1β hippocampus levels in a rat model of neuropathic pain |
spellingShingle |
A3 receptor agonist modulates IL-1β hippocampus levels in a rat model of neuropathic pain Cioato, Stefania Giotti adenosine A3 receptor cytokine DMSO IB-MECA neuropathic pain neurotrophin rats. |
title_short |
A3 receptor agonist modulates IL-1β hippocampus levels in a rat model of neuropathic pain |
title_full |
A3 receptor agonist modulates IL-1β hippocampus levels in a rat model of neuropathic pain |
title_fullStr |
A3 receptor agonist modulates IL-1β hippocampus levels in a rat model of neuropathic pain |
title_full_unstemmed |
A3 receptor agonist modulates IL-1β hippocampus levels in a rat model of neuropathic pain |
title_sort |
A3 receptor agonist modulates IL-1β hippocampus levels in a rat model of neuropathic pain |
author |
Cioato, Stefania Giotti |
author_facet |
Cioato, Stefania Giotti Medeiros, Liciane Fernandes Lopes, Bettega Costa Medeiros, Helouise Richardt Caumo, Wolnei Roesler, Rafael Torres, Iraci LS |
author_role |
author |
author2 |
Medeiros, Liciane Fernandes Lopes, Bettega Costa Medeiros, Helouise Richardt Caumo, Wolnei Roesler, Rafael Torres, Iraci LS |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Cioato, Stefania Giotti Medeiros, Liciane Fernandes Lopes, Bettega Costa Medeiros, Helouise Richardt Caumo, Wolnei Roesler, Rafael Torres, Iraci LS |
dc.subject.por.fl_str_mv |
adenosine A3 receptor cytokine DMSO IB-MECA neuropathic pain neurotrophin rats. |
topic |
adenosine A3 receptor cytokine DMSO IB-MECA neuropathic pain neurotrophin rats. |
description |
Introduction: Considering the lack of specific treatments to neuropathic pain, this study aimed to evaluate the effect of a single dose adenosine A3 receptor IB-MECA in the inflammatory and neurotrophic parameters of rats submitted to a neuropathic pain model. Methods: 64 adults male Wistar rats were used. Neuropathic pain was induced by the chronic constriction injury (CCI) of sciatic nerve and the treatment consisted in one dose of 0.5 μmol/kg of a selective agonist of adenosine A3 receptor IB-MECA dissolved in 3% DMSO; vehicle groups received DMSO 3% in saline; morphine groups received 5mg/kg Cerebral cortex and hippocampus IL-1β, BDNF and NGF levels were determined by ELISA assay. Results: The key finding was that a single dose of IB-MECA was able to modulate the IL-1β hippocampus levels CCI and the DMSO increased IL-1β and NGF hippocampus levels in sham animals; however, when the DMSO as an IB-MECA vehicle, this effect was not observed, indicating that IB-MECA was able to prevent the effect of DMSO. Conclusions: Considering that the IL-1β role in neuropathic pain is quite explored, as well as the hippocampus contributions, our result corroborates the relationship of A3 receptor and the chronic pain maintenance process. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0in; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman";}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes;}div.WordSection1 {page:WordSection1;} |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Avaliado por Pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufrgs.br/index.php/hcpa/article/view/116706 |
url |
https://seer.ufrgs.br/index.php/hcpa/article/view/116706 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.ufrgs.br/index.php/hcpa/article/view/116706/85500 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Clinical and Biomedical Research http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Clinical and Biomedical Research http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
HCPA/FAMED/UFRGS |
publisher.none.fl_str_mv |
HCPA/FAMED/UFRGS |
dc.source.none.fl_str_mv |
Clinical & Biomedical Research; Vol. 42 No. 2 (2022): Clinical & Biomedical Research Clinical and Biomedical Research; v. 42 n. 2 (2022): Clinical and Biomedical Research 2357-9730 reponame:Clinical and Biomedical Research instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Clinical and Biomedical Research |
collection |
Clinical and Biomedical Research |
repository.name.fl_str_mv |
Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
||cbr@hcpa.edu.br |
_version_ |
1799767056295395328 |