First reported genome of an mcr-9-mediated colistin-resistant Salmonella Typhimurium isolate from Brazilian livestock
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/218515 |
Resumo: | Objectives: To investigate the genetic context of colistin resistance in anmcr-9-harbouring Salmonella Typhimurium ST19 strain from swine in Brazil. Methods: Minimum inhibitory concentrations (MIC) to colistin were determined by broth microdilution. Whole-genome sequencing was performed on an Illumina MiSeq system, followed by de novo genome assembly using SPAdes 1.13.1. The draft genome sequence was annotated in Prokka using KBase online server. Downstream analyses for resistome and plasmid detection were performed using online tools available at the Center for Genomic Epidemiology. The strain was typed in silico using MLST 2.0. Phylogenetic analysis involving 24 other genomes of Salmonella Typhimurium ST19 and mcr-9-harbouring Salmonella Typhimurium isolated from humans, livestock and foodstuff in different regions was also performed. Results: Assembly of the draft genome resulted in 5245 protein-coding sequences, 14 rRNAs, 83 tRNAs and a GC content of 51.81%. The strain was identified as Salmonella Typhimurium ST19 harbouring a 265.5-kb pN1566-2 plasmid carrying genes encoding resistance to colistin (mcr-9.1), aminoglycosides (aadA1), tetracycline [tet(C)] and sulfonamides (sul1). Our findings indicate that the Salmonella Typhimurium ST19 strain in this study showed low genetic variability compared with Salmonella Typhimurium ST19 isolated from swine and poultry in Brazil, and was less related to those reported in other countries. Conclusions: This is the first reported genome of a phenotypically colistin-resistant Salmonella Typhimurium harbouring the mcr-9 variant in Brazilian livestock. This genome will aid global investigations on epidemiological and evolutionary aspects of plasmid-mediated colistin resistance and the role of colistin-resistant Salmonella Typhimurium ST19 lineage as a zoonotic pathogen. |
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Leite, Elma LimaAraujo, Wydemberg Jose deVieira, Tatiana ReginaZenato, Karoline SilvaVasconcelos, Priscylla CarvalhoCibulski, Samuel PauloGivisiez, Patrícia Emília NavesCardoso, Marisa Ribeiro de ItapemaOliveira, Celso José Bruno de2021-03-09T04:49:00Z20202213-7165http://hdl.handle.net/10183/218515001122303Objectives: To investigate the genetic context of colistin resistance in anmcr-9-harbouring Salmonella Typhimurium ST19 strain from swine in Brazil. Methods: Minimum inhibitory concentrations (MIC) to colistin were determined by broth microdilution. Whole-genome sequencing was performed on an Illumina MiSeq system, followed by de novo genome assembly using SPAdes 1.13.1. The draft genome sequence was annotated in Prokka using KBase online server. Downstream analyses for resistome and plasmid detection were performed using online tools available at the Center for Genomic Epidemiology. The strain was typed in silico using MLST 2.0. Phylogenetic analysis involving 24 other genomes of Salmonella Typhimurium ST19 and mcr-9-harbouring Salmonella Typhimurium isolated from humans, livestock and foodstuff in different regions was also performed. Results: Assembly of the draft genome resulted in 5245 protein-coding sequences, 14 rRNAs, 83 tRNAs and a GC content of 51.81%. The strain was identified as Salmonella Typhimurium ST19 harbouring a 265.5-kb pN1566-2 plasmid carrying genes encoding resistance to colistin (mcr-9.1), aminoglycosides (aadA1), tetracycline [tet(C)] and sulfonamides (sul1). Our findings indicate that the Salmonella Typhimurium ST19 strain in this study showed low genetic variability compared with Salmonella Typhimurium ST19 isolated from swine and poultry in Brazil, and was less related to those reported in other countries. Conclusions: This is the first reported genome of a phenotypically colistin-resistant Salmonella Typhimurium harbouring the mcr-9 variant in Brazilian livestock. This genome will aid global investigations on epidemiological and evolutionary aspects of plasmid-mediated colistin resistance and the role of colistin-resistant Salmonella Typhimurium ST19 lineage as a zoonotic pathogen.application/pdfengJournal of global antimicrobial resistance. Amsterdam. Vol. 23 (Dec. 2020), p. 394-397Salmonella typhimuriumSequenciamento completo do genomaResistência a antimicrobianosColistinaFilogeniaSuínosWhole-genome sequencingColistin resistancePolymyxinmcr-9First reported genome of an mcr-9-mediated colistin-resistant Salmonella Typhimurium isolate from Brazilian livestockEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001122303.pdf.txt001122303.pdf.txtExtracted Texttext/plain19682http://www.lume.ufrgs.br/bitstream/10183/218515/2/001122303.pdf.txtc578331a77418e1a2168755d05f557ebMD52ORIGINAL001122303.pdfTexto completo (inglês)application/pdf543578http://www.lume.ufrgs.br/bitstream/10183/218515/1/001122303.pdfcd10884cc7687b2dd16c2fc28e62b233MD5110183/2185152021-05-07 05:08:31.643381oai:www.lume.ufrgs.br:10183/218515Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2021-05-07T08:08:31Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
First reported genome of an mcr-9-mediated colistin-resistant Salmonella Typhimurium isolate from Brazilian livestock |
title |
First reported genome of an mcr-9-mediated colistin-resistant Salmonella Typhimurium isolate from Brazilian livestock |
spellingShingle |
First reported genome of an mcr-9-mediated colistin-resistant Salmonella Typhimurium isolate from Brazilian livestock Leite, Elma Lima Salmonella typhimurium Sequenciamento completo do genoma Resistência a antimicrobianos Colistina Filogenia Suínos Whole-genome sequencing Colistin resistance Polymyxin mcr-9 |
title_short |
First reported genome of an mcr-9-mediated colistin-resistant Salmonella Typhimurium isolate from Brazilian livestock |
title_full |
First reported genome of an mcr-9-mediated colistin-resistant Salmonella Typhimurium isolate from Brazilian livestock |
title_fullStr |
First reported genome of an mcr-9-mediated colistin-resistant Salmonella Typhimurium isolate from Brazilian livestock |
title_full_unstemmed |
First reported genome of an mcr-9-mediated colistin-resistant Salmonella Typhimurium isolate from Brazilian livestock |
title_sort |
First reported genome of an mcr-9-mediated colistin-resistant Salmonella Typhimurium isolate from Brazilian livestock |
author |
Leite, Elma Lima |
author_facet |
Leite, Elma Lima Araujo, Wydemberg Jose de Vieira, Tatiana Regina Zenato, Karoline Silva Vasconcelos, Priscylla Carvalho Cibulski, Samuel Paulo Givisiez, Patrícia Emília Naves Cardoso, Marisa Ribeiro de Itapema Oliveira, Celso José Bruno de |
author_role |
author |
author2 |
Araujo, Wydemberg Jose de Vieira, Tatiana Regina Zenato, Karoline Silva Vasconcelos, Priscylla Carvalho Cibulski, Samuel Paulo Givisiez, Patrícia Emília Naves Cardoso, Marisa Ribeiro de Itapema Oliveira, Celso José Bruno de |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Leite, Elma Lima Araujo, Wydemberg Jose de Vieira, Tatiana Regina Zenato, Karoline Silva Vasconcelos, Priscylla Carvalho Cibulski, Samuel Paulo Givisiez, Patrícia Emília Naves Cardoso, Marisa Ribeiro de Itapema Oliveira, Celso José Bruno de |
dc.subject.por.fl_str_mv |
Salmonella typhimurium Sequenciamento completo do genoma Resistência a antimicrobianos Colistina Filogenia Suínos |
topic |
Salmonella typhimurium Sequenciamento completo do genoma Resistência a antimicrobianos Colistina Filogenia Suínos Whole-genome sequencing Colistin resistance Polymyxin mcr-9 |
dc.subject.eng.fl_str_mv |
Whole-genome sequencing Colistin resistance Polymyxin mcr-9 |
description |
Objectives: To investigate the genetic context of colistin resistance in anmcr-9-harbouring Salmonella Typhimurium ST19 strain from swine in Brazil. Methods: Minimum inhibitory concentrations (MIC) to colistin were determined by broth microdilution. Whole-genome sequencing was performed on an Illumina MiSeq system, followed by de novo genome assembly using SPAdes 1.13.1. The draft genome sequence was annotated in Prokka using KBase online server. Downstream analyses for resistome and plasmid detection were performed using online tools available at the Center for Genomic Epidemiology. The strain was typed in silico using MLST 2.0. Phylogenetic analysis involving 24 other genomes of Salmonella Typhimurium ST19 and mcr-9-harbouring Salmonella Typhimurium isolated from humans, livestock and foodstuff in different regions was also performed. Results: Assembly of the draft genome resulted in 5245 protein-coding sequences, 14 rRNAs, 83 tRNAs and a GC content of 51.81%. The strain was identified as Salmonella Typhimurium ST19 harbouring a 265.5-kb pN1566-2 plasmid carrying genes encoding resistance to colistin (mcr-9.1), aminoglycosides (aadA1), tetracycline [tet(C)] and sulfonamides (sul1). Our findings indicate that the Salmonella Typhimurium ST19 strain in this study showed low genetic variability compared with Salmonella Typhimurium ST19 isolated from swine and poultry in Brazil, and was less related to those reported in other countries. Conclusions: This is the first reported genome of a phenotypically colistin-resistant Salmonella Typhimurium harbouring the mcr-9 variant in Brazilian livestock. This genome will aid global investigations on epidemiological and evolutionary aspects of plasmid-mediated colistin resistance and the role of colistin-resistant Salmonella Typhimurium ST19 lineage as a zoonotic pathogen. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2021-03-09T04:49:00Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/218515 |
dc.identifier.issn.pt_BR.fl_str_mv |
2213-7165 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001122303 |
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2213-7165 001122303 |
url |
http://hdl.handle.net/10183/218515 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of global antimicrobial resistance. Amsterdam. Vol. 23 (Dec. 2020), p. 394-397 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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