Genomics and phenotypical characterization of two new lytic bacteriophages for biocontrol of Salmonella enterica
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/220814 |
Resumo: | Aims: To perform the isolation, characterization and sequencing of the bacteriophages. To demonstrate that the bacteriophages can be used for biocontrol of different Salmonella enterica serovars. Study Design: This study was an experimental study. Place and Duration of Study: Bacteriology and Mycology Laboratory in the Veterinary Hospital at the Faculty of Agronomy and Veterinary Medicine of the University of Passo Fundo (FAMV/UPF), Biotechnology Center (CBiotec) of the Federal University of Paraíba (UFPB), Center for Microscopy and Microanalysis at the Faculty of Veterinary of the Federal University of Rio Grande do Sul (UFRGS), between January – September 2016. Methodology: Twelve Salmonella enterica serovars (S. Anatum, S. Agona, S. Brandenburg, S. Bredeney, S. Infantis, S. Lexington, S. Panama, S. Rissen, S. Schwarzengrund, S. Tennessee, S. Enteritidis ATCC 13076 and S. Typhimurium ATCC 14028) were selected to be the hosts. We isolate, purify, produce and determine the bacteriophage titers to verify the potential for lysis of these phages against the hosts. Having determined the action of the phages against the hosts, we performed the sequencing of the bacteriophages on the Illumina Mi-Seq platform and the morphology was performed by transmission electron microscopy (TEM). Results: We isolated, characterized and sequenced the genome of two new bacteriophages, Salmonella phage UPF_BP1, belonging to the family Podoviridae and Salmonella phage UPF_BP2, family Myoviridae. UPF_BP1 has lytic action against seven tested Salmonella enterica serovars, while UPF_BP2 has action against the twelve tested serovars. Conclusion: The two new bacteriophages have a lytic action against different Salmonella enterica serovars, feeding our expectations for the development of alternatives for the use of antimicrobials, being possible candidates for use as a biocontrol of Salmonella enterica in food, animals and the environment. |
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Pottker, Emanuele SerroWebber, BrunaCibulski, Samuel PauloZanella, RicardoGirardi, VivianeNúncio, Adriana Souto PereiraSantos, Luciana Ruschel dosNascimento, Vladimir Pinheiro doRodrigues, Laura Beatriz2021-05-13T04:26:14Z20202456-7043http://hdl.handle.net/10183/220814001122409Aims: To perform the isolation, characterization and sequencing of the bacteriophages. To demonstrate that the bacteriophages can be used for biocontrol of different Salmonella enterica serovars. Study Design: This study was an experimental study. Place and Duration of Study: Bacteriology and Mycology Laboratory in the Veterinary Hospital at the Faculty of Agronomy and Veterinary Medicine of the University of Passo Fundo (FAMV/UPF), Biotechnology Center (CBiotec) of the Federal University of Paraíba (UFPB), Center for Microscopy and Microanalysis at the Faculty of Veterinary of the Federal University of Rio Grande do Sul (UFRGS), between January – September 2016. Methodology: Twelve Salmonella enterica serovars (S. Anatum, S. Agona, S. Brandenburg, S. Bredeney, S. Infantis, S. Lexington, S. Panama, S. Rissen, S. Schwarzengrund, S. Tennessee, S. Enteritidis ATCC 13076 and S. Typhimurium ATCC 14028) were selected to be the hosts. We isolate, purify, produce and determine the bacteriophage titers to verify the potential for lysis of these phages against the hosts. Having determined the action of the phages against the hosts, we performed the sequencing of the bacteriophages on the Illumina Mi-Seq platform and the morphology was performed by transmission electron microscopy (TEM). Results: We isolated, characterized and sequenced the genome of two new bacteriophages, Salmonella phage UPF_BP1, belonging to the family Podoviridae and Salmonella phage UPF_BP2, family Myoviridae. UPF_BP1 has lytic action against seven tested Salmonella enterica serovars, while UPF_BP2 has action against the twelve tested serovars. Conclusion: The two new bacteriophages have a lytic action against different Salmonella enterica serovars, feeding our expectations for the development of alternatives for the use of antimicrobials, being possible candidates for use as a biocontrol of Salmonella enterica in food, animals and the environment.application/pdfengMicrobiology research journal international. Hooghly. Vol. 30, no. 10 (2020), MRJI.64596, p. 93-105BacteriófagosControle biológicoSalmonella entericaFagos de SalmonellaAnálise de sequência de DNAGenoma viralBacteriophagesGenomeSalmonella phage UPF_BP1Salmonella phage UPF_BP2BiocontrolGenomics and phenotypical characterization of two new lytic bacteriophages for biocontrol of Salmonella entericaEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001122409.pdf.txt001122409.pdf.txtExtracted Texttext/plain41712http://www.lume.ufrgs.br/bitstream/10183/220814/2/001122409.pdf.txte1656fcf0d3e6ea982e6cf9e28f9c679MD52ORIGINAL001122409.pdfTexto completo (inglês)application/pdf933257http://www.lume.ufrgs.br/bitstream/10183/220814/1/001122409.pdf6c02fc86b8395307404f0b5a32b9b00fMD5110183/2208142023-08-12 03:48:26.444387oai:www.lume.ufrgs.br:10183/220814Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-08-12T06:48:26Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Genomics and phenotypical characterization of two new lytic bacteriophages for biocontrol of Salmonella enterica |
title |
Genomics and phenotypical characterization of two new lytic bacteriophages for biocontrol of Salmonella enterica |
spellingShingle |
Genomics and phenotypical characterization of two new lytic bacteriophages for biocontrol of Salmonella enterica Pottker, Emanuele Serro Bacteriófagos Controle biológico Salmonella enterica Fagos de Salmonella Análise de sequência de DNA Genoma viral Bacteriophages Genome Salmonella phage UPF_BP1 Salmonella phage UPF_BP2 Biocontrol |
title_short |
Genomics and phenotypical characterization of two new lytic bacteriophages for biocontrol of Salmonella enterica |
title_full |
Genomics and phenotypical characterization of two new lytic bacteriophages for biocontrol of Salmonella enterica |
title_fullStr |
Genomics and phenotypical characterization of two new lytic bacteriophages for biocontrol of Salmonella enterica |
title_full_unstemmed |
Genomics and phenotypical characterization of two new lytic bacteriophages for biocontrol of Salmonella enterica |
title_sort |
Genomics and phenotypical characterization of two new lytic bacteriophages for biocontrol of Salmonella enterica |
author |
Pottker, Emanuele Serro |
author_facet |
Pottker, Emanuele Serro Webber, Bruna Cibulski, Samuel Paulo Zanella, Ricardo Girardi, Viviane Núncio, Adriana Souto Pereira Santos, Luciana Ruschel dos Nascimento, Vladimir Pinheiro do Rodrigues, Laura Beatriz |
author_role |
author |
author2 |
Webber, Bruna Cibulski, Samuel Paulo Zanella, Ricardo Girardi, Viviane Núncio, Adriana Souto Pereira Santos, Luciana Ruschel dos Nascimento, Vladimir Pinheiro do Rodrigues, Laura Beatriz |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pottker, Emanuele Serro Webber, Bruna Cibulski, Samuel Paulo Zanella, Ricardo Girardi, Viviane Núncio, Adriana Souto Pereira Santos, Luciana Ruschel dos Nascimento, Vladimir Pinheiro do Rodrigues, Laura Beatriz |
dc.subject.por.fl_str_mv |
Bacteriófagos Controle biológico Salmonella enterica Fagos de Salmonella Análise de sequência de DNA Genoma viral |
topic |
Bacteriófagos Controle biológico Salmonella enterica Fagos de Salmonella Análise de sequência de DNA Genoma viral Bacteriophages Genome Salmonella phage UPF_BP1 Salmonella phage UPF_BP2 Biocontrol |
dc.subject.eng.fl_str_mv |
Bacteriophages Genome Salmonella phage UPF_BP1 Salmonella phage UPF_BP2 Biocontrol |
description |
Aims: To perform the isolation, characterization and sequencing of the bacteriophages. To demonstrate that the bacteriophages can be used for biocontrol of different Salmonella enterica serovars. Study Design: This study was an experimental study. Place and Duration of Study: Bacteriology and Mycology Laboratory in the Veterinary Hospital at the Faculty of Agronomy and Veterinary Medicine of the University of Passo Fundo (FAMV/UPF), Biotechnology Center (CBiotec) of the Federal University of Paraíba (UFPB), Center for Microscopy and Microanalysis at the Faculty of Veterinary of the Federal University of Rio Grande do Sul (UFRGS), between January – September 2016. Methodology: Twelve Salmonella enterica serovars (S. Anatum, S. Agona, S. Brandenburg, S. Bredeney, S. Infantis, S. Lexington, S. Panama, S. Rissen, S. Schwarzengrund, S. Tennessee, S. Enteritidis ATCC 13076 and S. Typhimurium ATCC 14028) were selected to be the hosts. We isolate, purify, produce and determine the bacteriophage titers to verify the potential for lysis of these phages against the hosts. Having determined the action of the phages against the hosts, we performed the sequencing of the bacteriophages on the Illumina Mi-Seq platform and the morphology was performed by transmission electron microscopy (TEM). Results: We isolated, characterized and sequenced the genome of two new bacteriophages, Salmonella phage UPF_BP1, belonging to the family Podoviridae and Salmonella phage UPF_BP2, family Myoviridae. UPF_BP1 has lytic action against seven tested Salmonella enterica serovars, while UPF_BP2 has action against the twelve tested serovars. Conclusion: The two new bacteriophages have a lytic action against different Salmonella enterica serovars, feeding our expectations for the development of alternatives for the use of antimicrobials, being possible candidates for use as a biocontrol of Salmonella enterica in food, animals and the environment. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2021-05-13T04:26:14Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/220814 |
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2456-7043 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001122409 |
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2456-7043 001122409 |
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http://hdl.handle.net/10183/220814 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Microbiology research journal international. Hooghly. Vol. 30, no. 10 (2020), MRJI.64596, p. 93-105 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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