Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/218298 |
Resumo: | The ectonucleotidase pathway is an important metabolic source of extracellular adenosine. Adenosine has potent anticonvulsant effects on various models of epilepsy. One of these models is pentylenetetrazol (PTZ) kindling, in which repeated administration of subconvulsive doses of this drug induces progressive intensification of seizure activity. In this study, we examine the effect of a single convulsive injection (60 mg/kg, i.p.) or 10 successive (35 mg/kg, i.p.) injections of PTZ on synaptosomal ectonucleotidases. Our results have shown that no changes in ectonucleotidase activities were seen at 0, 1, and 24 h or at 5 days after a single convulsive PTZ injection. However, after PTZ-kindling, rats which were more resistant to seizure development presented an increase in ATP hydrolysis in synaptosomes from hippocampus and cerebral cortex (44% and 28%, respectively). These results suggest that changes in nucleotide hydrolysis may represent an important mechanism in the modulation of chronic epileptic activity in this model. |
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Bonan, Carla DeniseAmaral, Olavo BohrerRockenbach, Isabel CristinaWalz, RogerBattastini, Ana Maria OliveiraIzquierdo, Ivan AntonioSarkis, João José Freitas2021-03-02T04:15:38Z20000364-3190http://hdl.handle.net/10183/218298000299591The ectonucleotidase pathway is an important metabolic source of extracellular adenosine. Adenosine has potent anticonvulsant effects on various models of epilepsy. One of these models is pentylenetetrazol (PTZ) kindling, in which repeated administration of subconvulsive doses of this drug induces progressive intensification of seizure activity. In this study, we examine the effect of a single convulsive injection (60 mg/kg, i.p.) or 10 successive (35 mg/kg, i.p.) injections of PTZ on synaptosomal ectonucleotidases. Our results have shown that no changes in ectonucleotidase activities were seen at 0, 1, and 24 h or at 5 days after a single convulsive PTZ injection. However, after PTZ-kindling, rats which were more resistant to seizure development presented an increase in ATP hydrolysis in synaptosomes from hippocampus and cerebral cortex (44% and 28%, respectively). These results suggest that changes in nucleotide hydrolysis may represent an important mechanism in the modulation of chronic epileptic activity in this model.application/pdfengNeurochemical research. New York, NY. Vol. 25, n. 6 (2000), p. 775-779AdenosinaEpilepsiaExcitação neurológicaPentilenotetrazolEctonucleotidasesAdenosineEpilepsyKindlingPentylenetetrazolAltered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000299591.pdf.txt000299591.pdf.txtExtracted Texttext/plain0http://www.lume.ufrgs.br/bitstream/10183/218298/2/000299591.pdf.txtd41d8cd98f00b204e9800998ecf8427eMD52ORIGINAL000299591.pdfTexto completo (inglês)application/pdf1152505http://www.lume.ufrgs.br/bitstream/10183/218298/1/000299591.pdfa0292a02f958320569a815caf3671193MD5110183/2182982021-04-12 08:30:33.982403oai:www.lume.ufrgs.br:10183/218298Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-04-12T11:30:33Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes |
title |
Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes |
spellingShingle |
Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes Bonan, Carla Denise Adenosina Epilepsia Excitação neurológica Pentilenotetrazol Ectonucleotidases Adenosine Epilepsy Kindling Pentylenetetrazol |
title_short |
Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes |
title_full |
Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes |
title_fullStr |
Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes |
title_full_unstemmed |
Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes |
title_sort |
Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes |
author |
Bonan, Carla Denise |
author_facet |
Bonan, Carla Denise Amaral, Olavo Bohrer Rockenbach, Isabel Cristina Walz, Roger Battastini, Ana Maria Oliveira Izquierdo, Ivan Antonio Sarkis, João José Freitas |
author_role |
author |
author2 |
Amaral, Olavo Bohrer Rockenbach, Isabel Cristina Walz, Roger Battastini, Ana Maria Oliveira Izquierdo, Ivan Antonio Sarkis, João José Freitas |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Bonan, Carla Denise Amaral, Olavo Bohrer Rockenbach, Isabel Cristina Walz, Roger Battastini, Ana Maria Oliveira Izquierdo, Ivan Antonio Sarkis, João José Freitas |
dc.subject.por.fl_str_mv |
Adenosina Epilepsia Excitação neurológica Pentilenotetrazol |
topic |
Adenosina Epilepsia Excitação neurológica Pentilenotetrazol Ectonucleotidases Adenosine Epilepsy Kindling Pentylenetetrazol |
dc.subject.eng.fl_str_mv |
Ectonucleotidases Adenosine Epilepsy Kindling Pentylenetetrazol |
description |
The ectonucleotidase pathway is an important metabolic source of extracellular adenosine. Adenosine has potent anticonvulsant effects on various models of epilepsy. One of these models is pentylenetetrazol (PTZ) kindling, in which repeated administration of subconvulsive doses of this drug induces progressive intensification of seizure activity. In this study, we examine the effect of a single convulsive injection (60 mg/kg, i.p.) or 10 successive (35 mg/kg, i.p.) injections of PTZ on synaptosomal ectonucleotidases. Our results have shown that no changes in ectonucleotidase activities were seen at 0, 1, and 24 h or at 5 days after a single convulsive PTZ injection. However, after PTZ-kindling, rats which were more resistant to seizure development presented an increase in ATP hydrolysis in synaptosomes from hippocampus and cerebral cortex (44% and 28%, respectively). These results suggest that changes in nucleotide hydrolysis may represent an important mechanism in the modulation of chronic epileptic activity in this model. |
publishDate |
2000 |
dc.date.issued.fl_str_mv |
2000 |
dc.date.accessioned.fl_str_mv |
2021-03-02T04:15:38Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/218298 |
dc.identifier.issn.pt_BR.fl_str_mv |
0364-3190 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000299591 |
identifier_str_mv |
0364-3190 000299591 |
url |
http://hdl.handle.net/10183/218298 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Neurochemical research. New York, NY. Vol. 25, n. 6 (2000), p. 775-779 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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