Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes

Detalhes bibliográficos
Autor(a) principal: Bonan, Carla Denise
Data de Publicação: 2000
Outros Autores: Amaral, Olavo Bohrer, Rockenbach, Isabel Cristina, Walz, Roger, Battastini, Ana Maria Oliveira, Izquierdo, Ivan Antonio, Sarkis, João José Freitas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/218298
Resumo: The ectonucleotidase pathway is an important metabolic source of extracellular adenosine. Adenosine has potent anticonvulsant effects on various models of epilepsy. One of these models is pentylenetetrazol (PTZ) kindling, in which repeated administration of subconvulsive doses of this drug induces progressive intensification of seizure activity. In this study, we examine the effect of a single convulsive injection (60 mg/kg, i.p.) or 10 successive (35 mg/kg, i.p.) injections of PTZ on synaptosomal ectonucleotidases. Our results have shown that no changes in ectonucleotidase activities were seen at 0, 1, and 24 h or at 5 days after a single convulsive PTZ injection. However, after PTZ-kindling, rats which were more resistant to seizure development presented an increase in ATP hydrolysis in synaptosomes from hippocampus and cerebral cortex (44% and 28%, respectively). These results suggest that changes in nucleotide hydrolysis may represent an important mechanism in the modulation of chronic epileptic activity in this model.
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spelling Bonan, Carla DeniseAmaral, Olavo BohrerRockenbach, Isabel CristinaWalz, RogerBattastini, Ana Maria OliveiraIzquierdo, Ivan AntonioSarkis, João José Freitas2021-03-02T04:15:38Z20000364-3190http://hdl.handle.net/10183/218298000299591The ectonucleotidase pathway is an important metabolic source of extracellular adenosine. Adenosine has potent anticonvulsant effects on various models of epilepsy. One of these models is pentylenetetrazol (PTZ) kindling, in which repeated administration of subconvulsive doses of this drug induces progressive intensification of seizure activity. In this study, we examine the effect of a single convulsive injection (60 mg/kg, i.p.) or 10 successive (35 mg/kg, i.p.) injections of PTZ on synaptosomal ectonucleotidases. Our results have shown that no changes in ectonucleotidase activities were seen at 0, 1, and 24 h or at 5 days after a single convulsive PTZ injection. However, after PTZ-kindling, rats which were more resistant to seizure development presented an increase in ATP hydrolysis in synaptosomes from hippocampus and cerebral cortex (44% and 28%, respectively). These results suggest that changes in nucleotide hydrolysis may represent an important mechanism in the modulation of chronic epileptic activity in this model.application/pdfengNeurochemical research. New York, NY. Vol. 25, n. 6 (2000), p. 775-779AdenosinaEpilepsiaExcitação neurológicaPentilenotetrazolEctonucleotidasesAdenosineEpilepsyKindlingPentylenetetrazolAltered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000299591.pdf.txt000299591.pdf.txtExtracted Texttext/plain0http://www.lume.ufrgs.br/bitstream/10183/218298/2/000299591.pdf.txtd41d8cd98f00b204e9800998ecf8427eMD52ORIGINAL000299591.pdfTexto completo (inglês)application/pdf1152505http://www.lume.ufrgs.br/bitstream/10183/218298/1/000299591.pdfa0292a02f958320569a815caf3671193MD5110183/2182982021-04-12 08:30:33.982403oai:www.lume.ufrgs.br:10183/218298Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-04-12T11:30:33Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes
title Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes
spellingShingle Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes
Bonan, Carla Denise
Adenosina
Epilepsia
Excitação neurológica
Pentilenotetrazol
Ectonucleotidases
Adenosine
Epilepsy
Kindling
Pentylenetetrazol
title_short Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes
title_full Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes
title_fullStr Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes
title_full_unstemmed Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes
title_sort Altered ATP hydrolysis induced by pentylenetetrazol kindling in rat brain synaptosomes
author Bonan, Carla Denise
author_facet Bonan, Carla Denise
Amaral, Olavo Bohrer
Rockenbach, Isabel Cristina
Walz, Roger
Battastini, Ana Maria Oliveira
Izquierdo, Ivan Antonio
Sarkis, João José Freitas
author_role author
author2 Amaral, Olavo Bohrer
Rockenbach, Isabel Cristina
Walz, Roger
Battastini, Ana Maria Oliveira
Izquierdo, Ivan Antonio
Sarkis, João José Freitas
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bonan, Carla Denise
Amaral, Olavo Bohrer
Rockenbach, Isabel Cristina
Walz, Roger
Battastini, Ana Maria Oliveira
Izquierdo, Ivan Antonio
Sarkis, João José Freitas
dc.subject.por.fl_str_mv Adenosina
Epilepsia
Excitação neurológica
Pentilenotetrazol
topic Adenosina
Epilepsia
Excitação neurológica
Pentilenotetrazol
Ectonucleotidases
Adenosine
Epilepsy
Kindling
Pentylenetetrazol
dc.subject.eng.fl_str_mv Ectonucleotidases
Adenosine
Epilepsy
Kindling
Pentylenetetrazol
description The ectonucleotidase pathway is an important metabolic source of extracellular adenosine. Adenosine has potent anticonvulsant effects on various models of epilepsy. One of these models is pentylenetetrazol (PTZ) kindling, in which repeated administration of subconvulsive doses of this drug induces progressive intensification of seizure activity. In this study, we examine the effect of a single convulsive injection (60 mg/kg, i.p.) or 10 successive (35 mg/kg, i.p.) injections of PTZ on synaptosomal ectonucleotidases. Our results have shown that no changes in ectonucleotidase activities were seen at 0, 1, and 24 h or at 5 days after a single convulsive PTZ injection. However, after PTZ-kindling, rats which were more resistant to seizure development presented an increase in ATP hydrolysis in synaptosomes from hippocampus and cerebral cortex (44% and 28%, respectively). These results suggest that changes in nucleotide hydrolysis may represent an important mechanism in the modulation of chronic epileptic activity in this model.
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2021-03-02T04:15:38Z
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Neurochemical research. New York, NY. Vol. 25, n. 6 (2000), p. 775-779
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