Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/188355 |
Resumo: | It is widely known that maternal physical exercise is able to induce beneficial improvements in offspring cognition; however, the effects of paternal exercise have not been explored in detail. The present study was designed to evaluate the impact of paternal physical exercise on memory and learning, neuroplasticity and DNA methylation levels in the hippocampus of male offspring. Adult male Wistar rats were divided into two groups: sedentary or exercised fathers. The paternal preconception exercise protocol consisted of treadmill running, 20 minutes daily, 5 consecutive days per week for 22 days, while the mothers were not trained. After mating, paternal sperm was collected for global DNA methylation analysis. At postnatal day 53, the offspring were euthanized, and the hippocampus was dissected to measure cell survival by 5-bromo-2′-deoxiuridine and to determine the expression of synaptophysin, reelin, brain-derived neurotrophic factor and global DNA methylation levels. To measure spatial memory and learning changes in offspring, the Morris water maze paradigm was used. There was an improvement in spatial learning, as well as a significant decrease in hippocampal global DNA methylation levels in the offspring from exercised fathers compared with those from sedentary ones; however, no changes were observed in neuroplasticity biomarkers brain-derived neurotrophic factor, reelin and 5-bromo-2′-deoxiuridine. Finally, the global DNA methylation of paternal sperm was not significantly changed by physical exercise. These results suggest a link between paternal preconception physical activity and cognitive benefit, which may be associated with hippocampal epigenetic programming in male offspring. However, the biological mechanisms of this modulation remain unclear. |
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Spindler, Christiano de FigueiredoSegabinazi, EthianeMeireles, André Luís Ferreira dePiazza, Francele ValenteSantos, Filipe Mega dosSalvalaggio, Gabriela dos SantosAchaval-Elena, MatildeElsner, Viviane RostirolaMarcuzzo, Simone2019-01-30T02:33:17Z20191876-7958http://hdl.handle.net/10183/188355001083664It is widely known that maternal physical exercise is able to induce beneficial improvements in offspring cognition; however, the effects of paternal exercise have not been explored in detail. The present study was designed to evaluate the impact of paternal physical exercise on memory and learning, neuroplasticity and DNA methylation levels in the hippocampus of male offspring. Adult male Wistar rats were divided into two groups: sedentary or exercised fathers. The paternal preconception exercise protocol consisted of treadmill running, 20 minutes daily, 5 consecutive days per week for 22 days, while the mothers were not trained. After mating, paternal sperm was collected for global DNA methylation analysis. At postnatal day 53, the offspring were euthanized, and the hippocampus was dissected to measure cell survival by 5-bromo-2′-deoxiuridine and to determine the expression of synaptophysin, reelin, brain-derived neurotrophic factor and global DNA methylation levels. To measure spatial memory and learning changes in offspring, the Morris water maze paradigm was used. There was an improvement in spatial learning, as well as a significant decrease in hippocampal global DNA methylation levels in the offspring from exercised fathers compared with those from sedentary ones; however, no changes were observed in neuroplasticity biomarkers brain-derived neurotrophic factor, reelin and 5-bromo-2′-deoxiuridine. Finally, the global DNA methylation of paternal sperm was not significantly changed by physical exercise. These results suggest a link between paternal preconception physical activity and cognitive benefit, which may be associated with hippocampal epigenetic programming in male offspring. However, the biological mechanisms of this modulation remain unclear.application/pdfengNeural regeneration research. Mumbai. V. 14, no. 3 (2019), p. 491-500ExercícioMetilação de DNAHerança paternaHipocampoEpigeneticsPreconceptionMethylationLearningTreadmill runningFetal programmingInheritanceSpermNerve regenerationPaternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspringEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001083664.pdf.txt001083664.pdf.txtExtracted Texttext/plain63633http://www.lume.ufrgs.br/bitstream/10183/188355/2/001083664.pdf.txt36ac2ae8363262b9268ed5915856c21fMD52ORIGINAL001083664.pdfTexto completo (inglês)application/pdf989474http://www.lume.ufrgs.br/bitstream/10183/188355/1/001083664.pdf4cfd90faf15a569d70adaeded309bcffMD5110183/1883552019-01-31 02:33:12.830659oai:www.lume.ufrgs.br:10183/188355Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-01-31T04:33:12Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
title |
Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
spellingShingle |
Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring Spindler, Christiano de Figueiredo Exercício Metilação de DNA Herança paterna Hipocampo Epigenetics Preconception Methylation Learning Treadmill running Fetal programming Inheritance Sperm Nerve regeneration |
title_short |
Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
title_full |
Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
title_fullStr |
Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
title_full_unstemmed |
Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
title_sort |
Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring |
author |
Spindler, Christiano de Figueiredo |
author_facet |
Spindler, Christiano de Figueiredo Segabinazi, Ethiane Meireles, André Luís Ferreira de Piazza, Francele Valente Santos, Filipe Mega dos Salvalaggio, Gabriela dos Santos Achaval-Elena, Matilde Elsner, Viviane Rostirola Marcuzzo, Simone |
author_role |
author |
author2 |
Segabinazi, Ethiane Meireles, André Luís Ferreira de Piazza, Francele Valente Santos, Filipe Mega dos Salvalaggio, Gabriela dos Santos Achaval-Elena, Matilde Elsner, Viviane Rostirola Marcuzzo, Simone |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Spindler, Christiano de Figueiredo Segabinazi, Ethiane Meireles, André Luís Ferreira de Piazza, Francele Valente Santos, Filipe Mega dos Salvalaggio, Gabriela dos Santos Achaval-Elena, Matilde Elsner, Viviane Rostirola Marcuzzo, Simone |
dc.subject.por.fl_str_mv |
Exercício Metilação de DNA Herança paterna Hipocampo |
topic |
Exercício Metilação de DNA Herança paterna Hipocampo Epigenetics Preconception Methylation Learning Treadmill running Fetal programming Inheritance Sperm Nerve regeneration |
dc.subject.eng.fl_str_mv |
Epigenetics Preconception Methylation Learning Treadmill running Fetal programming Inheritance Sperm Nerve regeneration |
description |
It is widely known that maternal physical exercise is able to induce beneficial improvements in offspring cognition; however, the effects of paternal exercise have not been explored in detail. The present study was designed to evaluate the impact of paternal physical exercise on memory and learning, neuroplasticity and DNA methylation levels in the hippocampus of male offspring. Adult male Wistar rats were divided into two groups: sedentary or exercised fathers. The paternal preconception exercise protocol consisted of treadmill running, 20 minutes daily, 5 consecutive days per week for 22 days, while the mothers were not trained. After mating, paternal sperm was collected for global DNA methylation analysis. At postnatal day 53, the offspring were euthanized, and the hippocampus was dissected to measure cell survival by 5-bromo-2′-deoxiuridine and to determine the expression of synaptophysin, reelin, brain-derived neurotrophic factor and global DNA methylation levels. To measure spatial memory and learning changes in offspring, the Morris water maze paradigm was used. There was an improvement in spatial learning, as well as a significant decrease in hippocampal global DNA methylation levels in the offspring from exercised fathers compared with those from sedentary ones; however, no changes were observed in neuroplasticity biomarkers brain-derived neurotrophic factor, reelin and 5-bromo-2′-deoxiuridine. Finally, the global DNA methylation of paternal sperm was not significantly changed by physical exercise. These results suggest a link between paternal preconception physical activity and cognitive benefit, which may be associated with hippocampal epigenetic programming in male offspring. However, the biological mechanisms of this modulation remain unclear. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-01-30T02:33:17Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/188355 |
dc.identifier.issn.pt_BR.fl_str_mv |
1876-7958 |
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001083664 |
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http://hdl.handle.net/10183/188355 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Neural regeneration research. Mumbai. V. 14, no. 3 (2019), p. 491-500 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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