Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis

Detalhes bibliográficos
Autor(a) principal: Santos, Guilherme Brzoskowski dos
Data de Publicação: 2022
Outros Autores: Silva, Edileuza Danieli da, Kitano, Eduardo Shigueo, Battistella, Maria Eduarda, Monteiro, Karina Mariante, Lima, Jeferson Camargo de, Ferreira, Henrique Bunselmeyer, Serrano, Solange Maria de Toledo, Zaha, Arnaldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/267516
Resumo: Background: Most cystic echinococcosis cases in Southern Brazil are caused by Echinococcus granulosus and Echinococcus ortleppi. Proteomic studies of helminths have increased our knowledge about the molecular survival strategies that are used by parasites. Here, we surveyed the protein content of the hydatid fluid compartment in E. granulosus and E. ortleppi pulmonary bovine cysts to better describe and compare their molecular arsenal at the host-parasite interface. Methods: Hydatid fluid samples from three isolates of each species were analyzed using mass spectrometry-based proteomics (LC-MS/MS). In silico functional analyses of the identified proteins were performed to examine parasite survival strategies. Results: The identified hydatid fluid protein profiles showed a predominance of parasite proteins compared to host proteins that infiltrate the cysts. We identified 280 parasitic proteins from E. granulosus and 251 from E. ortleppi, including 52 parasitic proteins that were common to all hydatid fluid samples. The in silico functional analysis revealed important molecular functions and processes that are active in pulmonary cystic echinococcosis, such as adhesion, extracellular structures organization, development regulation, signaling transduction, and enzyme activity.Conclusions: The protein profiles described here provide evidence of important mechanisms related to basic cellular processes and functions that act at the host-parasite interface in cystic echinococcosis. The molecular tools used by E. granulosus and E. ortleppi for survival within the host are potential targets for new therapeutic approaches to treat cystic echinococcosis and other larval cestodiases.
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spelling Santos, Guilherme Brzoskowski dosSilva, Edileuza Danieli daKitano, Eduardo ShigueoBattistella, Maria EduardaMonteiro, Karina MarianteLima, Jeferson Camargo deFerreira, Henrique BunselmeyerSerrano, Solange Maria de ToledoZaha, Arnaldo2023-11-23T03:34:38Z20221756-3305http://hdl.handle.net/10183/267516001175292Background: Most cystic echinococcosis cases in Southern Brazil are caused by Echinococcus granulosus and Echinococcus ortleppi. Proteomic studies of helminths have increased our knowledge about the molecular survival strategies that are used by parasites. Here, we surveyed the protein content of the hydatid fluid compartment in E. granulosus and E. ortleppi pulmonary bovine cysts to better describe and compare their molecular arsenal at the host-parasite interface. Methods: Hydatid fluid samples from three isolates of each species were analyzed using mass spectrometry-based proteomics (LC-MS/MS). In silico functional analyses of the identified proteins were performed to examine parasite survival strategies. Results: The identified hydatid fluid protein profiles showed a predominance of parasite proteins compared to host proteins that infiltrate the cysts. We identified 280 parasitic proteins from E. granulosus and 251 from E. ortleppi, including 52 parasitic proteins that were common to all hydatid fluid samples. The in silico functional analysis revealed important molecular functions and processes that are active in pulmonary cystic echinococcosis, such as adhesion, extracellular structures organization, development regulation, signaling transduction, and enzyme activity.Conclusions: The protein profiles described here provide evidence of important mechanisms related to basic cellular processes and functions that act at the host-parasite interface in cystic echinococcosis. The molecular tools used by E. granulosus and E. ortleppi for survival within the host are potential targets for new therapeutic approaches to treat cystic echinococcosis and other larval cestodiases.application/pdfengParasites & Vectors. London. Vol. 15 (2022), e99, 19 p.Echinococcus granulosusEchinococcus ortleppiSecretomeHydatid fuidParasite proteomicsHost-parasite interfaceProteomic profling of hydatid fuid from pulmonary cystic echinococcosisEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001175292.pdf.txt001175292.pdf.txtExtracted Texttext/plain89035http://www.lume.ufrgs.br/bitstream/10183/267516/2/001175292.pdf.txt6a0b0d78a19cc3a49a1947533ba65fe1MD52ORIGINAL001175292.pdfTexto completo (inglês)application/pdf1990170http://www.lume.ufrgs.br/bitstream/10183/267516/1/001175292.pdfdc614e623e20e976a1b4060d3aec5624MD5110183/2675162023-11-24 04:25:08.356391oai:www.lume.ufrgs.br:10183/267516Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-11-24T06:25:08Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis
title Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis
spellingShingle Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis
Santos, Guilherme Brzoskowski dos
Echinococcus granulosus
Echinococcus ortleppi
Secretome
Hydatid fuid
Parasite proteomics
Host-parasite interface
title_short Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis
title_full Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis
title_fullStr Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis
title_full_unstemmed Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis
title_sort Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis
author Santos, Guilherme Brzoskowski dos
author_facet Santos, Guilherme Brzoskowski dos
Silva, Edileuza Danieli da
Kitano, Eduardo Shigueo
Battistella, Maria Eduarda
Monteiro, Karina Mariante
Lima, Jeferson Camargo de
Ferreira, Henrique Bunselmeyer
Serrano, Solange Maria de Toledo
Zaha, Arnaldo
author_role author
author2 Silva, Edileuza Danieli da
Kitano, Eduardo Shigueo
Battistella, Maria Eduarda
Monteiro, Karina Mariante
Lima, Jeferson Camargo de
Ferreira, Henrique Bunselmeyer
Serrano, Solange Maria de Toledo
Zaha, Arnaldo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Guilherme Brzoskowski dos
Silva, Edileuza Danieli da
Kitano, Eduardo Shigueo
Battistella, Maria Eduarda
Monteiro, Karina Mariante
Lima, Jeferson Camargo de
Ferreira, Henrique Bunselmeyer
Serrano, Solange Maria de Toledo
Zaha, Arnaldo
dc.subject.por.fl_str_mv Echinococcus granulosus
Echinococcus ortleppi
topic Echinococcus granulosus
Echinococcus ortleppi
Secretome
Hydatid fuid
Parasite proteomics
Host-parasite interface
dc.subject.eng.fl_str_mv Secretome
Hydatid fuid
Parasite proteomics
Host-parasite interface
description Background: Most cystic echinococcosis cases in Southern Brazil are caused by Echinococcus granulosus and Echinococcus ortleppi. Proteomic studies of helminths have increased our knowledge about the molecular survival strategies that are used by parasites. Here, we surveyed the protein content of the hydatid fluid compartment in E. granulosus and E. ortleppi pulmonary bovine cysts to better describe and compare their molecular arsenal at the host-parasite interface. Methods: Hydatid fluid samples from three isolates of each species were analyzed using mass spectrometry-based proteomics (LC-MS/MS). In silico functional analyses of the identified proteins were performed to examine parasite survival strategies. Results: The identified hydatid fluid protein profiles showed a predominance of parasite proteins compared to host proteins that infiltrate the cysts. We identified 280 parasitic proteins from E. granulosus and 251 from E. ortleppi, including 52 parasitic proteins that were common to all hydatid fluid samples. The in silico functional analysis revealed important molecular functions and processes that are active in pulmonary cystic echinococcosis, such as adhesion, extracellular structures organization, development regulation, signaling transduction, and enzyme activity.Conclusions: The protein profiles described here provide evidence of important mechanisms related to basic cellular processes and functions that act at the host-parasite interface in cystic echinococcosis. The molecular tools used by E. granulosus and E. ortleppi for survival within the host are potential targets for new therapeutic approaches to treat cystic echinococcosis and other larval cestodiases.
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-11-23T03:34:38Z
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dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.nrb.pt_BR.fl_str_mv 001175292
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url http://hdl.handle.net/10183/267516
dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Parasites & Vectors. London. Vol. 15 (2022), e99, 19 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
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