Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/267516 |
Resumo: | Background: Most cystic echinococcosis cases in Southern Brazil are caused by Echinococcus granulosus and Echinococcus ortleppi. Proteomic studies of helminths have increased our knowledge about the molecular survival strategies that are used by parasites. Here, we surveyed the protein content of the hydatid fluid compartment in E. granulosus and E. ortleppi pulmonary bovine cysts to better describe and compare their molecular arsenal at the host-parasite interface. Methods: Hydatid fluid samples from three isolates of each species were analyzed using mass spectrometry-based proteomics (LC-MS/MS). In silico functional analyses of the identified proteins were performed to examine parasite survival strategies. Results: The identified hydatid fluid protein profiles showed a predominance of parasite proteins compared to host proteins that infiltrate the cysts. We identified 280 parasitic proteins from E. granulosus and 251 from E. ortleppi, including 52 parasitic proteins that were common to all hydatid fluid samples. The in silico functional analysis revealed important molecular functions and processes that are active in pulmonary cystic echinococcosis, such as adhesion, extracellular structures organization, development regulation, signaling transduction, and enzyme activity.Conclusions: The protein profiles described here provide evidence of important mechanisms related to basic cellular processes and functions that act at the host-parasite interface in cystic echinococcosis. The molecular tools used by E. granulosus and E. ortleppi for survival within the host are potential targets for new therapeutic approaches to treat cystic echinococcosis and other larval cestodiases. |
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Santos, Guilherme Brzoskowski dosSilva, Edileuza Danieli daKitano, Eduardo ShigueoBattistella, Maria EduardaMonteiro, Karina MarianteLima, Jeferson Camargo deFerreira, Henrique BunselmeyerSerrano, Solange Maria de ToledoZaha, Arnaldo2023-11-23T03:34:38Z20221756-3305http://hdl.handle.net/10183/267516001175292Background: Most cystic echinococcosis cases in Southern Brazil are caused by Echinococcus granulosus and Echinococcus ortleppi. Proteomic studies of helminths have increased our knowledge about the molecular survival strategies that are used by parasites. Here, we surveyed the protein content of the hydatid fluid compartment in E. granulosus and E. ortleppi pulmonary bovine cysts to better describe and compare their molecular arsenal at the host-parasite interface. Methods: Hydatid fluid samples from three isolates of each species were analyzed using mass spectrometry-based proteomics (LC-MS/MS). In silico functional analyses of the identified proteins were performed to examine parasite survival strategies. Results: The identified hydatid fluid protein profiles showed a predominance of parasite proteins compared to host proteins that infiltrate the cysts. We identified 280 parasitic proteins from E. granulosus and 251 from E. ortleppi, including 52 parasitic proteins that were common to all hydatid fluid samples. The in silico functional analysis revealed important molecular functions and processes that are active in pulmonary cystic echinococcosis, such as adhesion, extracellular structures organization, development regulation, signaling transduction, and enzyme activity.Conclusions: The protein profiles described here provide evidence of important mechanisms related to basic cellular processes and functions that act at the host-parasite interface in cystic echinococcosis. The molecular tools used by E. granulosus and E. ortleppi for survival within the host are potential targets for new therapeutic approaches to treat cystic echinococcosis and other larval cestodiases.application/pdfengParasites & Vectors. London. Vol. 15 (2022), e99, 19 p.Echinococcus granulosusEchinococcus ortleppiSecretomeHydatid fuidParasite proteomicsHost-parasite interfaceProteomic profling of hydatid fuid from pulmonary cystic echinococcosisEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001175292.pdf.txt001175292.pdf.txtExtracted Texttext/plain89035http://www.lume.ufrgs.br/bitstream/10183/267516/2/001175292.pdf.txt6a0b0d78a19cc3a49a1947533ba65fe1MD52ORIGINAL001175292.pdfTexto completo (inglês)application/pdf1990170http://www.lume.ufrgs.br/bitstream/10183/267516/1/001175292.pdfdc614e623e20e976a1b4060d3aec5624MD5110183/2675162023-11-24 04:25:08.356391oai:www.lume.ufrgs.br:10183/267516Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-11-24T06:25:08Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis |
title |
Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis |
spellingShingle |
Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis Santos, Guilherme Brzoskowski dos Echinococcus granulosus Echinococcus ortleppi Secretome Hydatid fuid Parasite proteomics Host-parasite interface |
title_short |
Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis |
title_full |
Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis |
title_fullStr |
Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis |
title_full_unstemmed |
Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis |
title_sort |
Proteomic profling of hydatid fuid from pulmonary cystic echinococcosis |
author |
Santos, Guilherme Brzoskowski dos |
author_facet |
Santos, Guilherme Brzoskowski dos Silva, Edileuza Danieli da Kitano, Eduardo Shigueo Battistella, Maria Eduarda Monteiro, Karina Mariante Lima, Jeferson Camargo de Ferreira, Henrique Bunselmeyer Serrano, Solange Maria de Toledo Zaha, Arnaldo |
author_role |
author |
author2 |
Silva, Edileuza Danieli da Kitano, Eduardo Shigueo Battistella, Maria Eduarda Monteiro, Karina Mariante Lima, Jeferson Camargo de Ferreira, Henrique Bunselmeyer Serrano, Solange Maria de Toledo Zaha, Arnaldo |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Guilherme Brzoskowski dos Silva, Edileuza Danieli da Kitano, Eduardo Shigueo Battistella, Maria Eduarda Monteiro, Karina Mariante Lima, Jeferson Camargo de Ferreira, Henrique Bunselmeyer Serrano, Solange Maria de Toledo Zaha, Arnaldo |
dc.subject.por.fl_str_mv |
Echinococcus granulosus Echinococcus ortleppi |
topic |
Echinococcus granulosus Echinococcus ortleppi Secretome Hydatid fuid Parasite proteomics Host-parasite interface |
dc.subject.eng.fl_str_mv |
Secretome Hydatid fuid Parasite proteomics Host-parasite interface |
description |
Background: Most cystic echinococcosis cases in Southern Brazil are caused by Echinococcus granulosus and Echinococcus ortleppi. Proteomic studies of helminths have increased our knowledge about the molecular survival strategies that are used by parasites. Here, we surveyed the protein content of the hydatid fluid compartment in E. granulosus and E. ortleppi pulmonary bovine cysts to better describe and compare their molecular arsenal at the host-parasite interface. Methods: Hydatid fluid samples from three isolates of each species were analyzed using mass spectrometry-based proteomics (LC-MS/MS). In silico functional analyses of the identified proteins were performed to examine parasite survival strategies. Results: The identified hydatid fluid protein profiles showed a predominance of parasite proteins compared to host proteins that infiltrate the cysts. We identified 280 parasitic proteins from E. granulosus and 251 from E. ortleppi, including 52 parasitic proteins that were common to all hydatid fluid samples. The in silico functional analysis revealed important molecular functions and processes that are active in pulmonary cystic echinococcosis, such as adhesion, extracellular structures organization, development regulation, signaling transduction, and enzyme activity.Conclusions: The protein profiles described here provide evidence of important mechanisms related to basic cellular processes and functions that act at the host-parasite interface in cystic echinococcosis. The molecular tools used by E. granulosus and E. ortleppi for survival within the host are potential targets for new therapeutic approaches to treat cystic echinococcosis and other larval cestodiases. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022 |
dc.date.accessioned.fl_str_mv |
2023-11-23T03:34:38Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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http://hdl.handle.net/10183/267516 |
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1756-3305 |
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001175292 |
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http://hdl.handle.net/10183/267516 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Parasites & Vectors. London. Vol. 15 (2022), e99, 19 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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