The inhibitory effects of phenolic and terpenoid compounds from Baccharis trimera in siha cells : differences in their activity and mechanism of action

• Autor(a) principal: Oliveira, Cristiane Bernardes de
• Data de Publicação: 2013
• Outros Autores: Comunello, Lucimara Nardi, Maciel, Érica Santos, Giubel, Scheron Rathke, Bruno, Alessandra Nejar, Chiela, Eduardo Cremonese Filippi, Lenz, Guido, Gnoatto, Simone Cristina Baggio, Buffon, Andreia, Gosmann, Grace
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/267627
• topic: Baccharis trimera; Terpenóides; Compostos fenólicos; Neoplasias do colo do útero; Phenolic compounds; Terpenoids; Cervical cancer; Necrosis; Apoptosis

Baccharis trimera is used in folk medicine as a tea for digestive and liver diseases. It possesses anti-inflammatory and antioxidant properties that are related to the presence of phenolic compounds. The aim of this work was to investigate the anti-proliferative properties of phenolic (PHE) and terpenoid (SAP) compounds from B. trimera on human cervical cancer. The treatment of SiHa cells with PHE for 24 h suppressed colony formation in a dose-dependent manner, inhibited proliferation and inhibited cell motility. Although SAP inhibited the proliferation of SiHa cells in a dose-dependent manner, it increased colony formation and did not inhibit cell motility. PHE and SAP also promoted a significant increase in lactate dehydrogenase levels in the culture medium in a dose-dependent manner, indicating a loss of cell membrane integrity. Moreover, PHE promoted necrotic cell death, whereas SAP induced apoptosis. These compounds are new anticancer prototypes due their significant anticancer activity demonstrated herein.