Whole-genome sequencing-based characterization of Streptomyces sp. 6(4) : focus on natural product

• Autor(a) principal: Borba, Marcela Proença
• Data de Publicação: 2023
• Outros Autores: Witusk, João Paulo Duarte, Cunha, Débora Marchesan, Morales, Daiana de Lima, Martins, Andreza Francisco, Van der Sand, Sueli Terezinha
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/259243
• topic: Sequenciamento completo do genoma; Streptomyces; Antifúngicos; Filogenia; Actinomycetes; Biosynthetic gene cluster; Genome mining; Phytopathogenic fungi; Secondary metabolites

We have sequenced the whole genome of Streptomyces sp. 6(4) isolated from tomato roots that presents antifungal activity against phytopathogenic fungi, mainly Bipolaris sorokiniana. The genome has almost 7Mb and 3368 hypothetical proteins that were analysed and characterized in Uniprot with the emphasis on biological compounds. Multilocus sequence typing (MLST) analyses were performed in an effort to characterize and identify this isolate, resulting in a new sequence type (ST), classified as ST64. Phenetic and phylogenetic trees were constructed to investigate Streptomyces sp. 6(4) evolution and sequence similarity, and the isolate is a strain closer to Streptomyces prasinus and Streptomyces viridosporus. It is known that the genus Streptomyces possess huge metabolic capacity with the presence of cryptic genes. These genes are usually present in clusters, which are responsible for the production of diverse natural products, mainly antibiotics. In addition, 6(4) showed 11 biosynthetic gene clusters through antiSMASH, including 3 polyketide synthase (PKS) and non-ribosomal peptide synthase (NRPS) type clusters.