MAEWEST expression in flower development of two petunia species
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/266330 |
Resumo: | Changes in flower morphology may influence the frequency and specificity of animal visitors. In Petunia (Solanaceae), adaptation to different pollinators is one of the factors leading to species diversification within the genus. This study provides evidence that differential expression patterns of MAWEWEST (MAW) homologs in different Petunia species may be associated with adaptive changes in floral morphology. The Petunia × hybrida MAW gene belongs to the WOX (WUSCHEL-related homeobox) transcription factor family and has been identified as a controller of petal fusion during corolla formation. We analyzed the expression patterns of P. inflata and P. axillaris MAW orthologs (PiMAW and PaMAW, respectively) by reverse transcriptase polymerase chain reaction (RT-PCR), reverse transcription–quantitative PCR (qRT-PCR) and in situ hybridization in different tissues and different developmental stages of flowers in both species. The spatial expression patterns of PiMAW and PaMAW were similar in P. inflata and P. axillaris. Nevertheless, PaMAW expression level in P. axillaris was higher during the late bud development stage as compared to PiMAW in P. inflata. This work represents an expansion of petunia developmental research to wild accessions. |
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Segatto, Ana Lúcia AnversaZolet, Andreia Carina TurchettoAizza, Lilian Cristina B.Bello, Carolina C. MonteDornelas, Marcelo C.Margis, RogerioFreitas, Loreta Brandão de2023-10-27T03:27:44Z20131422-0067http://hdl.handle.net/10183/266330000893154Changes in flower morphology may influence the frequency and specificity of animal visitors. In Petunia (Solanaceae), adaptation to different pollinators is one of the factors leading to species diversification within the genus. This study provides evidence that differential expression patterns of MAWEWEST (MAW) homologs in different Petunia species may be associated with adaptive changes in floral morphology. The Petunia × hybrida MAW gene belongs to the WOX (WUSCHEL-related homeobox) transcription factor family and has been identified as a controller of petal fusion during corolla formation. We analyzed the expression patterns of P. inflata and P. axillaris MAW orthologs (PiMAW and PaMAW, respectively) by reverse transcriptase polymerase chain reaction (RT-PCR), reverse transcription–quantitative PCR (qRT-PCR) and in situ hybridization in different tissues and different developmental stages of flowers in both species. The spatial expression patterns of PiMAW and PaMAW were similar in P. inflata and P. axillaris. Nevertheless, PaMAW expression level in P. axillaris was higher during the late bud development stage as compared to PiMAW in P. inflata. This work represents an expansion of petunia developmental research to wild accessions.application/pdfengInternational journal of molecular sciences. Basel. Vol. 14, no. 7 (July 2013), p. 13796-13807Petunia inflataPetunia axillarisMAEWEST expression in flower development of two petunia speciesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000893154.pdf.txt000893154.pdf.txtExtracted Texttext/plain31928http://www.lume.ufrgs.br/bitstream/10183/266330/2/000893154.pdf.txtefe435efaf68ee648633090a20eee7e2MD52ORIGINAL000893154.pdfTexto completo (inglês)application/pdf1259054http://www.lume.ufrgs.br/bitstream/10183/266330/1/000893154.pdf6a328145f56671b859959f1c35d768d9MD5110183/2663302023-10-28 03:32:30.90613oai:www.lume.ufrgs.br:10183/266330Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-10-28T06:32:30Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
MAEWEST expression in flower development of two petunia species |
title |
MAEWEST expression in flower development of two petunia species |
spellingShingle |
MAEWEST expression in flower development of two petunia species Segatto, Ana Lúcia Anversa Petunia inflata Petunia axillaris |
title_short |
MAEWEST expression in flower development of two petunia species |
title_full |
MAEWEST expression in flower development of two petunia species |
title_fullStr |
MAEWEST expression in flower development of two petunia species |
title_full_unstemmed |
MAEWEST expression in flower development of two petunia species |
title_sort |
MAEWEST expression in flower development of two petunia species |
author |
Segatto, Ana Lúcia Anversa |
author_facet |
Segatto, Ana Lúcia Anversa Zolet, Andreia Carina Turchetto Aizza, Lilian Cristina B. Bello, Carolina C. Monte Dornelas, Marcelo C. Margis, Rogerio Freitas, Loreta Brandão de |
author_role |
author |
author2 |
Zolet, Andreia Carina Turchetto Aizza, Lilian Cristina B. Bello, Carolina C. Monte Dornelas, Marcelo C. Margis, Rogerio Freitas, Loreta Brandão de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Segatto, Ana Lúcia Anversa Zolet, Andreia Carina Turchetto Aizza, Lilian Cristina B. Bello, Carolina C. Monte Dornelas, Marcelo C. Margis, Rogerio Freitas, Loreta Brandão de |
dc.subject.por.fl_str_mv |
Petunia inflata Petunia axillaris |
topic |
Petunia inflata Petunia axillaris |
description |
Changes in flower morphology may influence the frequency and specificity of animal visitors. In Petunia (Solanaceae), adaptation to different pollinators is one of the factors leading to species diversification within the genus. This study provides evidence that differential expression patterns of MAWEWEST (MAW) homologs in different Petunia species may be associated with adaptive changes in floral morphology. The Petunia × hybrida MAW gene belongs to the WOX (WUSCHEL-related homeobox) transcription factor family and has been identified as a controller of petal fusion during corolla formation. We analyzed the expression patterns of P. inflata and P. axillaris MAW orthologs (PiMAW and PaMAW, respectively) by reverse transcriptase polymerase chain reaction (RT-PCR), reverse transcription–quantitative PCR (qRT-PCR) and in situ hybridization in different tissues and different developmental stages of flowers in both species. The spatial expression patterns of PiMAW and PaMAW were similar in P. inflata and P. axillaris. Nevertheless, PaMAW expression level in P. axillaris was higher during the late bud development stage as compared to PiMAW in P. inflata. This work represents an expansion of petunia developmental research to wild accessions. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2023-10-27T03:27:44Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/266330 |
dc.identifier.issn.pt_BR.fl_str_mv |
1422-0067 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000893154 |
identifier_str_mv |
1422-0067 000893154 |
url |
http://hdl.handle.net/10183/266330 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
International journal of molecular sciences. Basel. Vol. 14, no. 7 (July 2013), p. 13796-13807 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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Repositório Institucional da UFRGS |
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Repositório Institucional da UFRGS |
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