Anti-Candida activity of Tripodanthus acutifolius (Loranthaceae), mechanism of action and toxicity parameters
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/234198 |
Resumo: | The extensive use of azoles antifungals against infections caused by Candida species has been contributing to the selection of resistant strains to this antifungal class, demonstrating that the prospection of new antifungal agents is essential and urgent. Tripodanthus acutifolius (Loranthaceae) is a plant widely used in folk medicine with reported antimicrobial activity. In this context, this study aimed to evaluate the antifungal potential and the mechanisms of action of the crude methanolic extract (CME) of T. acutifolius leaves against Candida species, as well as evaluate their toxicity parameters. As results, the phytochemical characterization of CME suggested the presence of four phenolic compounds as well as a tripodantoside compound. The CME presented minimum inhibitory concentration (MIC) between 16 to 64 μg mL-1 , with antifungal action possibly occurring in the fungal cell wall. In addition, the CME showed a synergic effect in combination with fluconazole (FLC). The CME demonstrated no mucosal irritation or tissue damage at all tested concentrations, as well as no cytotoxicity at the MIC values. This study is unprecedented and suggests that T. acutifolius is a new promising source for the development of anti-Candida agents on its own or as chemosensitizer associated to FLC. |
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Leal, Valéria LouzadaMachado, Gabriella da Rosa MonteKliemann, MarieleTeixeira, Mário LettieriSchneider, Rosana de Cassia de SouzaSilva, Chana de Medeiros daFuentefria, Alexandre Meneghello2022-01-19T04:36:47Z20212316-9281http://hdl.handle.net/10183/234198001135920The extensive use of azoles antifungals against infections caused by Candida species has been contributing to the selection of resistant strains to this antifungal class, demonstrating that the prospection of new antifungal agents is essential and urgent. Tripodanthus acutifolius (Loranthaceae) is a plant widely used in folk medicine with reported antimicrobial activity. In this context, this study aimed to evaluate the antifungal potential and the mechanisms of action of the crude methanolic extract (CME) of T. acutifolius leaves against Candida species, as well as evaluate their toxicity parameters. As results, the phytochemical characterization of CME suggested the presence of four phenolic compounds as well as a tripodantoside compound. The CME presented minimum inhibitory concentration (MIC) between 16 to 64 μg mL-1 , with antifungal action possibly occurring in the fungal cell wall. In addition, the CME showed a synergic effect in combination with fluconazole (FLC). The CME demonstrated no mucosal irritation or tissue damage at all tested concentrations, as well as no cytotoxicity at the MIC values. This study is unprecedented and suggests that T. acutifolius is a new promising source for the development of anti-Candida agents on its own or as chemosensitizer associated to FLC.application/pdfengScientific electronic archives. Rondonópolis (MT). Vol. 13, n. 6 (June 2021), p. 37-48AntifúngicosCandidaSinergismo farmacológicoFluconazolAntifungal agentsCandida spp.Tripodanthus acutifoliusSynergismFluconazoleAnti-Candida activity of Tripodanthus acutifolius (Loranthaceae), mechanism of action and toxicity parametersinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001135920.pdf.txt001135920.pdf.txtExtracted Texttext/plain50597http://www.lume.ufrgs.br/bitstream/10183/234198/2/001135920.pdf.txte4201820a811359f3c6fdb9437a7e322MD52ORIGINAL001135920.pdfTexto completo (inglês)application/pdf775014http://www.lume.ufrgs.br/bitstream/10183/234198/1/001135920.pdf4a6e0f0cd6671020248c025263ee77a0MD5110183/2341982022-02-22 04:58:07.262012oai:www.lume.ufrgs.br:10183/234198Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-02-22T07:58:07Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Anti-Candida activity of Tripodanthus acutifolius (Loranthaceae), mechanism of action and toxicity parameters |
title |
Anti-Candida activity of Tripodanthus acutifolius (Loranthaceae), mechanism of action and toxicity parameters |
spellingShingle |
Anti-Candida activity of Tripodanthus acutifolius (Loranthaceae), mechanism of action and toxicity parameters Leal, Valéria Louzada Antifúngicos Candida Sinergismo farmacológico Fluconazol Antifungal agents Candida spp. Tripodanthus acutifolius Synergism Fluconazole |
title_short |
Anti-Candida activity of Tripodanthus acutifolius (Loranthaceae), mechanism of action and toxicity parameters |
title_full |
Anti-Candida activity of Tripodanthus acutifolius (Loranthaceae), mechanism of action and toxicity parameters |
title_fullStr |
Anti-Candida activity of Tripodanthus acutifolius (Loranthaceae), mechanism of action and toxicity parameters |
title_full_unstemmed |
Anti-Candida activity of Tripodanthus acutifolius (Loranthaceae), mechanism of action and toxicity parameters |
title_sort |
Anti-Candida activity of Tripodanthus acutifolius (Loranthaceae), mechanism of action and toxicity parameters |
author |
Leal, Valéria Louzada |
author_facet |
Leal, Valéria Louzada Machado, Gabriella da Rosa Monte Kliemann, Mariele Teixeira, Mário Lettieri Schneider, Rosana de Cassia de Souza Silva, Chana de Medeiros da Fuentefria, Alexandre Meneghello |
author_role |
author |
author2 |
Machado, Gabriella da Rosa Monte Kliemann, Mariele Teixeira, Mário Lettieri Schneider, Rosana de Cassia de Souza Silva, Chana de Medeiros da Fuentefria, Alexandre Meneghello |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Leal, Valéria Louzada Machado, Gabriella da Rosa Monte Kliemann, Mariele Teixeira, Mário Lettieri Schneider, Rosana de Cassia de Souza Silva, Chana de Medeiros da Fuentefria, Alexandre Meneghello |
dc.subject.por.fl_str_mv |
Antifúngicos Candida Sinergismo farmacológico Fluconazol |
topic |
Antifúngicos Candida Sinergismo farmacológico Fluconazol Antifungal agents Candida spp. Tripodanthus acutifolius Synergism Fluconazole |
dc.subject.eng.fl_str_mv |
Antifungal agents Candida spp. Tripodanthus acutifolius Synergism Fluconazole |
description |
The extensive use of azoles antifungals against infections caused by Candida species has been contributing to the selection of resistant strains to this antifungal class, demonstrating that the prospection of new antifungal agents is essential and urgent. Tripodanthus acutifolius (Loranthaceae) is a plant widely used in folk medicine with reported antimicrobial activity. In this context, this study aimed to evaluate the antifungal potential and the mechanisms of action of the crude methanolic extract (CME) of T. acutifolius leaves against Candida species, as well as evaluate their toxicity parameters. As results, the phytochemical characterization of CME suggested the presence of four phenolic compounds as well as a tripodantoside compound. The CME presented minimum inhibitory concentration (MIC) between 16 to 64 μg mL-1 , with antifungal action possibly occurring in the fungal cell wall. In addition, the CME showed a synergic effect in combination with fluconazole (FLC). The CME demonstrated no mucosal irritation or tissue damage at all tested concentrations, as well as no cytotoxicity at the MIC values. This study is unprecedented and suggests that T. acutifolius is a new promising source for the development of anti-Candida agents on its own or as chemosensitizer associated to FLC. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021 |
dc.date.accessioned.fl_str_mv |
2022-01-19T04:36:47Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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publishedVersion |
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http://hdl.handle.net/10183/234198 |
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2316-9281 |
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001135920 |
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http://hdl.handle.net/10183/234198 |
dc.language.iso.fl_str_mv |
eng |
language |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Scientific electronic archives. Rondonópolis (MT). Vol. 13, n. 6 (June 2021), p. 37-48 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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