Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/77916 |
Resumo: | Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers, and it can be considered the most efficient method for the study of genetic divergence in strawberry. The most promising crosses, based on the genetic divergence estimated from the RAPD and ISSR molecular data were between the Tudla and Ventana and the Oso Grande and Ventana cultivars, respectively. |
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Morales, Rafael Gustavo FerreiraResende, Juliano Tadeu VilelaFaria, Marcos VenturaAndrade, Marcela CarvalhoResende, Luciane VilelaDelatorre, Carla AndreaSilva, Paulo Roberto da2013-09-11T01:45:59Z20110103-9016http://hdl.handle.net/10183/77916000856700Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers, and it can be considered the most efficient method for the study of genetic divergence in strawberry. The most promising crosses, based on the genetic divergence estimated from the RAPD and ISSR molecular data were between the Tudla and Ventana and the Oso Grande and Ventana cultivars, respectively.application/pdfengScientia Agricola. Piracicaba. Vol. 68, n.6 (nov./dez. 2011), p. 665-670MorangoMarcadores molecularesMelhoramento vegetalFragariaAnanassaPlant breedingGenetic divergenceMolecular markersGenetic similarity among strawberry cultivars assessed by RAPD and ISSR markersinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000856700.pdf000856700.pdfTexto completo (inglês)application/pdf152146http://www.lume.ufrgs.br/bitstream/10183/77916/1/000856700.pdfb75332020efb7fb12b548cb3f8520684MD51TEXT000856700.pdf.txt000856700.pdf.txtExtracted Texttext/plain28257http://www.lume.ufrgs.br/bitstream/10183/77916/2/000856700.pdf.txtaab8b4719a7b5ba370fdc9e2847ce2cdMD52THUMBNAIL000856700.pdf.jpg000856700.pdf.jpgGenerated Thumbnailimage/jpeg1999http://www.lume.ufrgs.br/bitstream/10183/77916/3/000856700.pdf.jpg84366e2eb0557e1c5b21beaa7991be23MD5310183/779162024-08-28 06:41:33.626832oai:www.lume.ufrgs.br:10183/77916Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-08-28T09:41:33Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
title |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
spellingShingle |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers Morales, Rafael Gustavo Ferreira Morango Marcadores moleculares Melhoramento vegetal Fragaria Ananassa Plant breeding Genetic divergence Molecular markers |
title_short |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
title_full |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
title_fullStr |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
title_full_unstemmed |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
title_sort |
Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers |
author |
Morales, Rafael Gustavo Ferreira |
author_facet |
Morales, Rafael Gustavo Ferreira Resende, Juliano Tadeu Vilela Faria, Marcos Ventura Andrade, Marcela Carvalho Resende, Luciane Vilela Delatorre, Carla Andrea Silva, Paulo Roberto da |
author_role |
author |
author2 |
Resende, Juliano Tadeu Vilela Faria, Marcos Ventura Andrade, Marcela Carvalho Resende, Luciane Vilela Delatorre, Carla Andrea Silva, Paulo Roberto da |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Morales, Rafael Gustavo Ferreira Resende, Juliano Tadeu Vilela Faria, Marcos Ventura Andrade, Marcela Carvalho Resende, Luciane Vilela Delatorre, Carla Andrea Silva, Paulo Roberto da |
dc.subject.por.fl_str_mv |
Morango Marcadores moleculares Melhoramento vegetal |
topic |
Morango Marcadores moleculares Melhoramento vegetal Fragaria Ananassa Plant breeding Genetic divergence Molecular markers |
dc.subject.eng.fl_str_mv |
Fragaria Ananassa Plant breeding Genetic divergence Molecular markers |
description |
Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers, and it can be considered the most efficient method for the study of genetic divergence in strawberry. The most promising crosses, based on the genetic divergence estimated from the RAPD and ISSR molecular data were between the Tudla and Ventana and the Oso Grande and Ventana cultivars, respectively. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2013-09-11T01:45:59Z |
dc.type.driver.fl_str_mv |
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http://hdl.handle.net/10183/77916 |
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0103-9016 |
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000856700 |
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http://hdl.handle.net/10183/77916 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Scientia Agricola. Piracicaba. Vol. 68, n.6 (nov./dez. 2011), p. 665-670 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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