Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

Detalhes bibliográficos
Autor(a) principal: Morales, Rafael Gustavo Ferreira
Data de Publicação: 2011
Outros Autores: Resende, Juliano Tadeu Vilela, Faria, Marcos Ventura, Andrade, Marcela Carvalho, Resende, Luciane Vilela, Delatorre, Carla Andrea, Silva, Paulo Roberto da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/77916
Resumo: Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers, and it can be considered the most efficient method for the study of genetic divergence in strawberry. The most promising crosses, based on the genetic divergence estimated from the RAPD and ISSR molecular data were between the Tudla and Ventana and the Oso Grande and Ventana cultivars, respectively.
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spelling Morales, Rafael Gustavo FerreiraResende, Juliano Tadeu VilelaFaria, Marcos VenturaAndrade, Marcela CarvalhoResende, Luciane VilelaDelatorre, Carla AndreaSilva, Paulo Roberto da2013-09-11T01:45:59Z20110103-9016http://hdl.handle.net/10183/77916000856700Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers, and it can be considered the most efficient method for the study of genetic divergence in strawberry. The most promising crosses, based on the genetic divergence estimated from the RAPD and ISSR molecular data were between the Tudla and Ventana and the Oso Grande and Ventana cultivars, respectively.application/pdfengScientia Agricola. Piracicaba. Vol. 68, n.6 (nov./dez. 2011), p. 665-670MorangoMarcadores molecularesMelhoramento vegetalFragariaAnanassaPlant breedingGenetic divergenceMolecular markersGenetic similarity among strawberry cultivars assessed by RAPD and ISSR markersinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000856700.pdf000856700.pdfTexto completo (inglês)application/pdf152146http://www.lume.ufrgs.br/bitstream/10183/77916/1/000856700.pdfb75332020efb7fb12b548cb3f8520684MD51TEXT000856700.pdf.txt000856700.pdf.txtExtracted Texttext/plain28257http://www.lume.ufrgs.br/bitstream/10183/77916/2/000856700.pdf.txtaab8b4719a7b5ba370fdc9e2847ce2cdMD52THUMBNAIL000856700.pdf.jpg000856700.pdf.jpgGenerated Thumbnailimage/jpeg1999http://www.lume.ufrgs.br/bitstream/10183/77916/3/000856700.pdf.jpg84366e2eb0557e1c5b21beaa7991be23MD5310183/779162024-08-28 06:41:33.626832oai:www.lume.ufrgs.br:10183/77916Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-08-28T09:41:33Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
title Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
spellingShingle Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
Morales, Rafael Gustavo Ferreira
Morango
Marcadores moleculares
Melhoramento vegetal
Fragaria
Ananassa
Plant breeding
Genetic divergence
Molecular markers
title_short Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
title_full Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
title_fullStr Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
title_full_unstemmed Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
title_sort Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
author Morales, Rafael Gustavo Ferreira
author_facet Morales, Rafael Gustavo Ferreira
Resende, Juliano Tadeu Vilela
Faria, Marcos Ventura
Andrade, Marcela Carvalho
Resende, Luciane Vilela
Delatorre, Carla Andrea
Silva, Paulo Roberto da
author_role author
author2 Resende, Juliano Tadeu Vilela
Faria, Marcos Ventura
Andrade, Marcela Carvalho
Resende, Luciane Vilela
Delatorre, Carla Andrea
Silva, Paulo Roberto da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Morales, Rafael Gustavo Ferreira
Resende, Juliano Tadeu Vilela
Faria, Marcos Ventura
Andrade, Marcela Carvalho
Resende, Luciane Vilela
Delatorre, Carla Andrea
Silva, Paulo Roberto da
dc.subject.por.fl_str_mv Morango
Marcadores moleculares
Melhoramento vegetal
topic Morango
Marcadores moleculares
Melhoramento vegetal
Fragaria
Ananassa
Plant breeding
Genetic divergence
Molecular markers
dc.subject.eng.fl_str_mv Fragaria
Ananassa
Plant breeding
Genetic divergence
Molecular markers
description Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers, and it can be considered the most efficient method for the study of genetic divergence in strawberry. The most promising crosses, based on the genetic divergence estimated from the RAPD and ISSR molecular data were between the Tudla and Ventana and the Oso Grande and Ventana cultivars, respectively.
publishDate 2011
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dc.relation.ispartof.pt_BR.fl_str_mv Scientia Agricola. Piracicaba. Vol. 68, n.6 (nov./dez. 2011), p. 665-670
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