Determinación de zearalenona por cromatografía líquida de alta resolución : normalización de un nuevo método para raciones especiales para cerdos

Detalhes bibliográficos
Autor(a) principal: Cover, Janaína Susan
Data de Publicação: 2010
Outros Autores: Teixeira, Mário Lettieri, Souza, Luís Flávio Oliveira, Fuentefria, Alexandre Meneghello
Tipo de documento: Artigo
Idioma: spa
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/71658
Resumo: The aim of this work was to offer a new method of high performance liquid chromatography (HPLC) to evaluate commercial swine rations (CSR) contaminated by zearalenone (ZEA). After ZEA extraction and purification from CSR, the samples were eluted with acetonitrile, methanol and water solvent system. The results indicated that the proposed method showed to be rapid and efficient for the detection and quantification of ZEA in CSR, since its recovery was 102.62%, it offered excellent precision with a coefficient of variation of 0.9992. Furthermore, it is also proposed a as a biocontrol assay for micotoxigenic fungi isolated and maintained in the laboratory. The test was performed with the killer yeast Trichosporum insectorum CBS 10422 against Fusarium sp and Aspergillus flavus, which demonstrated to be effective against the latter.
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spelling Cover, Janaína SusanTeixeira, Mário LettieriSouza, Luís Flávio OliveiraFuentefria, Alexandre Meneghello2013-05-17T01:43:08Z20100716-1549http://hdl.handle.net/10183/71658000762379The aim of this work was to offer a new method of high performance liquid chromatography (HPLC) to evaluate commercial swine rations (CSR) contaminated by zearalenone (ZEA). After ZEA extraction and purification from CSR, the samples were eluted with acetonitrile, methanol and water solvent system. The results indicated that the proposed method showed to be rapid and efficient for the detection and quantification of ZEA in CSR, since its recovery was 102.62%, it offered excellent precision with a coefficient of variation of 0.9992. Furthermore, it is also proposed a as a biocontrol assay for micotoxigenic fungi isolated and maintained in the laboratory. The test was performed with the killer yeast Trichosporum insectorum CBS 10422 against Fusarium sp and Aspergillus flavus, which demonstrated to be effective against the latter.El propósito de este artículo es ofrecer un nuevo método de cromatografía líquida de alta resolución (CLAR) para evaluar las raciones especiales para cerdos (REC) contaminado con zearalenona (ZEA). Después de la extracción y purificación de ZEA, las muestras se eluyeron con acetonitrilo, metanol y agua del sistema disolvente. Los resultados indican que el método pro¬puesto demostró ser rápido y eficaz para la detección y cuantificación de ZEA en REC, ya que sus indicadores se presentan capaces de recuperación de 102,62%, además de ofrece una excelente precisión, con un coeficiente de variación de 0,9992. Por otra parte, también se propone una prueba de control biológico de hongos micotoxige¬nic aislados y mantenidos en el laboratorio. La prueba se realizó con la levadura killer Trichosporum insectorum CBS10422 contra Fusarium sp y Aspergillus flavus, mostrando eficaces sólo contra Aspergillus.application/pdfspaRevista chilena de nutrición. Santiago, Chile. Vol. 37, n. 3 (Sept. 2010), p. 369-375ZearalenonaMicotoxinasCromatografia liquida de alta eficienciaZearalenoneHPLCBiocontrolCLARControl biológicoDeterminación de zearalenona por cromatografía líquida de alta resolución : normalización de un nuevo método para raciones especiales para cerdosZearalenone determination in high performance liquid chromatography : a new standardization method for commercial swine rationsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000762379.pdf000762379.pdfTexto completo (inglês)application/pdf1100434http://www.lume.ufrgs.br/bitstream/10183/71658/1/000762379.pdfbc7b268cd54b047338de7f7cf89717dfMD51TEXT000762379.pdf.txt000762379.pdf.txtExtracted Texttext/plain21028http://www.lume.ufrgs.br/bitstream/10183/71658/2/000762379.pdf.txt625907001ef84f89fd9fd26f569a63c6MD52THUMBNAIL000762379.pdf.jpg000762379.pdf.jpgGenerated Thumbnailimage/jpeg2253http://www.lume.ufrgs.br/bitstream/10183/71658/3/000762379.pdf.jpg6141be13c4e052aae5ba2e29ca6dfdd1MD5310183/716582018-10-17 07:42:00.155oai:www.lume.ufrgs.br:10183/71658Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-17T10:42Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Determinación de zearalenona por cromatografía líquida de alta resolución : normalización de un nuevo método para raciones especiales para cerdos
dc.title.alternative.en.fl_str_mv Zearalenone determination in high performance liquid chromatography : a new standardization method for commercial swine rations
title Determinación de zearalenona por cromatografía líquida de alta resolución : normalización de un nuevo método para raciones especiales para cerdos
spellingShingle Determinación de zearalenona por cromatografía líquida de alta resolución : normalización de un nuevo método para raciones especiales para cerdos
Cover, Janaína Susan
Zearalenona
Micotoxinas
Cromatografia liquida de alta eficiencia
Zearalenone
HPLC
Biocontrol
CLAR
Control biológico
title_short Determinación de zearalenona por cromatografía líquida de alta resolución : normalización de un nuevo método para raciones especiales para cerdos
title_full Determinación de zearalenona por cromatografía líquida de alta resolución : normalización de un nuevo método para raciones especiales para cerdos
title_fullStr Determinación de zearalenona por cromatografía líquida de alta resolución : normalización de un nuevo método para raciones especiales para cerdos
title_full_unstemmed Determinación de zearalenona por cromatografía líquida de alta resolución : normalización de un nuevo método para raciones especiales para cerdos
title_sort Determinación de zearalenona por cromatografía líquida de alta resolución : normalización de un nuevo método para raciones especiales para cerdos
author Cover, Janaína Susan
author_facet Cover, Janaína Susan
Teixeira, Mário Lettieri
Souza, Luís Flávio Oliveira
Fuentefria, Alexandre Meneghello
author_role author
author2 Teixeira, Mário Lettieri
Souza, Luís Flávio Oliveira
Fuentefria, Alexandre Meneghello
author2_role author
author
author
dc.contributor.author.fl_str_mv Cover, Janaína Susan
Teixeira, Mário Lettieri
Souza, Luís Flávio Oliveira
Fuentefria, Alexandre Meneghello
dc.subject.por.fl_str_mv Zearalenona
Micotoxinas
Cromatografia liquida de alta eficiencia
topic Zearalenona
Micotoxinas
Cromatografia liquida de alta eficiencia
Zearalenone
HPLC
Biocontrol
CLAR
Control biológico
dc.subject.eng.fl_str_mv Zearalenone
HPLC
Biocontrol
dc.subject.spa.fl_str_mv CLAR
Control biológico
description The aim of this work was to offer a new method of high performance liquid chromatography (HPLC) to evaluate commercial swine rations (CSR) contaminated by zearalenone (ZEA). After ZEA extraction and purification from CSR, the samples were eluted with acetonitrile, methanol and water solvent system. The results indicated that the proposed method showed to be rapid and efficient for the detection and quantification of ZEA in CSR, since its recovery was 102.62%, it offered excellent precision with a coefficient of variation of 0.9992. Furthermore, it is also proposed a as a biocontrol assay for micotoxigenic fungi isolated and maintained in the laboratory. The test was performed with the killer yeast Trichosporum insectorum CBS 10422 against Fusarium sp and Aspergillus flavus, which demonstrated to be effective against the latter.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2013-05-17T01:43:08Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/71658
dc.identifier.issn.pt_BR.fl_str_mv 0716-1549
dc.identifier.nrb.pt_BR.fl_str_mv 000762379
identifier_str_mv 0716-1549
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url http://hdl.handle.net/10183/71658
dc.language.iso.fl_str_mv spa
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dc.relation.ispartof.pt_BR.fl_str_mv Revista chilena de nutrición. Santiago, Chile. Vol. 37, n. 3 (Sept. 2010), p. 369-375
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