The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/87938 |
Resumo: | This report demonstrates the usefulness of PCR for the genes spaS and sboA as a means of identifying Bacillus strains with a potential to produce subtilin and subtilosin A. One collection strain and five Bacillus spp. isolated from aquatic environments in the Amazon basin were screened by PCR using primers for sboA and spaS designed specifically for this study. The sequences of the PCR products showed elevated homology with previously described spaS and sboA genes. Antimicrobial peptides were isolated from culture supernatants and analyzed by mass spectrometry. For all samples, the mass spectra revealed clusters with peaks at m/z 3300-3500 Da, corresponding to subtilosin A, subtilin and isoforms of these peptides. These results suggest that the antimicrobial activity of these strains may be associated with the production of subtilosin A and/or subtilin. The PCR used here was efficient in identifying novel Bacillus strains with the essential genes for producing subtilosin A and subtilin. |
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Velho, Renata VoltoliniBasso, Ana PaulaSegalin, JéfersonMedina, Luis Fernando da CostaBrandelli, Adriano2014-02-28T01:50:29Z20131415-4757http://hdl.handle.net/10183/87938000876437This report demonstrates the usefulness of PCR for the genes spaS and sboA as a means of identifying Bacillus strains with a potential to produce subtilin and subtilosin A. One collection strain and five Bacillus spp. isolated from aquatic environments in the Amazon basin were screened by PCR using primers for sboA and spaS designed specifically for this study. The sequences of the PCR products showed elevated homology with previously described spaS and sboA genes. Antimicrobial peptides were isolated from culture supernatants and analyzed by mass spectrometry. For all samples, the mass spectra revealed clusters with peaks at m/z 3300-3500 Da, corresponding to subtilosin A, subtilin and isoforms of these peptides. These results suggest that the antimicrobial activity of these strains may be associated with the production of subtilosin A and/or subtilin. The PCR used here was efficient in identifying novel Bacillus strains with the essential genes for producing subtilosin A and subtilin.application/pdfengGenetics and molecular biology. Vol. 36, n. 1 (Mar. 2013), p. 101-104BacilosBacteriocinaPolimerase : Reação em cadeiaReação em cadeia da polimeraseBacillusBacillusbacteriocinpolymerase chain reactionsubtilinsubtilosin AThe presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basininfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000876437.pdf000876437.pdfTexto completo (inglês)application/pdf642789http://www.lume.ufrgs.br/bitstream/10183/87938/1/000876437.pdf5e04fe41383969e4c338bcb8c51bc4a0MD51TEXT000876437.pdf.txt000876437.pdf.txtExtracted Texttext/plain15732http://www.lume.ufrgs.br/bitstream/10183/87938/2/000876437.pdf.txt3ad4c89951f5f9df511d9b8f8fe25265MD52THUMBNAIL000876437.pdf.jpg000876437.pdf.jpgGenerated Thumbnailimage/jpeg1833http://www.lume.ufrgs.br/bitstream/10183/87938/3/000876437.pdf.jpg91f815211827ece4c7f9e96b853db2bcMD5310183/879382021-07-09 04:35:10.891607oai:www.lume.ufrgs.br:10183/87938Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-07-09T07:35:10Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin |
title |
The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin |
spellingShingle |
The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin Velho, Renata Voltolini Bacilos Bacteriocina Polimerase : Reação em cadeia Reação em cadeia da polimerase Bacillus Bacillus bacteriocin polymerase chain reaction subtilin subtilosin A |
title_short |
The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin |
title_full |
The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin |
title_fullStr |
The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin |
title_full_unstemmed |
The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin |
title_sort |
The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin |
author |
Velho, Renata Voltolini |
author_facet |
Velho, Renata Voltolini Basso, Ana Paula Segalin, Jéferson Medina, Luis Fernando da Costa Brandelli, Adriano |
author_role |
author |
author2 |
Basso, Ana Paula Segalin, Jéferson Medina, Luis Fernando da Costa Brandelli, Adriano |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Velho, Renata Voltolini Basso, Ana Paula Segalin, Jéferson Medina, Luis Fernando da Costa Brandelli, Adriano |
dc.subject.por.fl_str_mv |
Bacilos Bacteriocina Polimerase : Reação em cadeia Reação em cadeia da polimerase Bacillus |
topic |
Bacilos Bacteriocina Polimerase : Reação em cadeia Reação em cadeia da polimerase Bacillus Bacillus bacteriocin polymerase chain reaction subtilin subtilosin A |
dc.subject.eng.fl_str_mv |
Bacillus bacteriocin polymerase chain reaction subtilin subtilosin A |
description |
This report demonstrates the usefulness of PCR for the genes spaS and sboA as a means of identifying Bacillus strains with a potential to produce subtilin and subtilosin A. One collection strain and five Bacillus spp. isolated from aquatic environments in the Amazon basin were screened by PCR using primers for sboA and spaS designed specifically for this study. The sequences of the PCR products showed elevated homology with previously described spaS and sboA genes. Antimicrobial peptides were isolated from culture supernatants and analyzed by mass spectrometry. For all samples, the mass spectra revealed clusters with peaks at m/z 3300-3500 Da, corresponding to subtilosin A, subtilin and isoforms of these peptides. These results suggest that the antimicrobial activity of these strains may be associated with the production of subtilosin A and/or subtilin. The PCR used here was efficient in identifying novel Bacillus strains with the essential genes for producing subtilosin A and subtilin. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2014-02-28T01:50:29Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/87938 |
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1415-4757 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000876437 |
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http://hdl.handle.net/10183/87938 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Genetics and molecular biology. Vol. 36, n. 1 (Mar. 2013), p. 101-104 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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