Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome

Detalhes bibliográficos
Autor(a) principal: Araujo, Gabriella Oliveira de
Data de Publicação: 2020
Outros Autores: Huff, Rosana, Favarini, Marina Ochoa, Mann, Michele Bertoni, Peters, Felipe Bortolotto, Frazzon, Jeverson, Frazzon, Ana Paula Guedes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/229520
Resumo: Enterococci are ubiquitous microorganisms present in various environments and within the gastrointestinal tracts of humans and other animals. Notably, fecal enterococci are suitable indicators for monitoring antimicrobial resistance dissemination. Resistant bacterial strains recovered from the fecal samples of wild animals can highlight important aspects of environmental disturbances. In this report, we investigated antimicrobial susceptibility as well as resistance and virulence genes in fecal enterococci isolated from wild Pampas foxes (Lycalopex gymnocercus) (n = 5) and Geoffroy's cats (Leopardus geoffroyi) (n = 4) in the Brazilian Pampa biome. Enterococci were isolated from eight out of nine fecal samples and Enterococcus faecalis was identified in both animals. However, E. faecium and E. durans were only detected in Pampas foxes, while E. hirae was only detected in Geoffroy's cats. Antimicrobial susceptibility analysis showed resistance to rifampicin (94%), erythromycin (72.6%), ciprofloxacin/norfloxacin (40%), streptomycin (38%), and tetracycline (26%). The high frequency of multidrug-resistant enterococci (66%) isolated in this study is a matter of concern since these are wild animals with no history of therapeutic antibiotic exposure. The tetM/tetL and msrC/ermB genes were detected in most tetracycline- and erythromycin-resistant enterococci, respectively. The gelE, ace, agg, esp, and clyA virulence genes were also detected in enterococci. In conclusion, our data suggest that habitat fragmentation and anthropogenic activities in the Pampa biome may contribute to high frequencies of multidrug-resistant enterococci in the gut communities of wild Pampas foxes and Geoffroy's cats. To the best of the authors' knowledge, this is the first report of antimicrobial-resistant enterococci in the Pampa biome.
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spelling Araujo, Gabriella Oliveira deHuff, RosanaFavarini, Marina OchoaMann, Michele BertoniPeters, Felipe BortolottoFrazzon, JeversonFrazzon, Ana Paula Guedes2021-09-03T04:25:43Z20202297-1769http://hdl.handle.net/10183/229520001130796Enterococci are ubiquitous microorganisms present in various environments and within the gastrointestinal tracts of humans and other animals. Notably, fecal enterococci are suitable indicators for monitoring antimicrobial resistance dissemination. Resistant bacterial strains recovered from the fecal samples of wild animals can highlight important aspects of environmental disturbances. In this report, we investigated antimicrobial susceptibility as well as resistance and virulence genes in fecal enterococci isolated from wild Pampas foxes (Lycalopex gymnocercus) (n = 5) and Geoffroy's cats (Leopardus geoffroyi) (n = 4) in the Brazilian Pampa biome. Enterococci were isolated from eight out of nine fecal samples and Enterococcus faecalis was identified in both animals. However, E. faecium and E. durans were only detected in Pampas foxes, while E. hirae was only detected in Geoffroy's cats. Antimicrobial susceptibility analysis showed resistance to rifampicin (94%), erythromycin (72.6%), ciprofloxacin/norfloxacin (40%), streptomycin (38%), and tetracycline (26%). The high frequency of multidrug-resistant enterococci (66%) isolated in this study is a matter of concern since these are wild animals with no history of therapeutic antibiotic exposure. The tetM/tetL and msrC/ermB genes were detected in most tetracycline- and erythromycin-resistant enterococci, respectively. The gelE, ace, agg, esp, and clyA virulence genes were also detected in enterococci. In conclusion, our data suggest that habitat fragmentation and anthropogenic activities in the Pampa biome may contribute to high frequencies of multidrug-resistant enterococci in the gut communities of wild Pampas foxes and Geoffroy's cats. To the best of the authors' knowledge, this is the first report of antimicrobial-resistant enterococci in the Pampa biome.application/pdfengFrontiers in veterinary science. Lausanne. Vol. 7 (Dec. 2020), 606377, 10 p.EnterococcusLycalopex gymnocercusFelidaeResistência microbiana a medicamentosFatores de virulênciaEnterococcus spp.Pampa biomeWildlife animalsPampas foxGeoffroy’s catMultidrug-resistanceVirulence factorsAntibiotic resistance genesMultidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biomeEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001130796.pdf.txt001130796.pdf.txtExtracted Texttext/plain53599http://www.lume.ufrgs.br/bitstream/10183/229520/2/001130796.pdf.txtced0e2512d0ee237df4f5505694dc2a4MD52ORIGINAL001130796.pdfTexto completo (inglês)application/pdf756515http://www.lume.ufrgs.br/bitstream/10183/229520/1/001130796.pdf81279f5965c8fb06d16f61f5c64f8332MD5110183/2295202021-09-19 04:31:16.856504oai:www.lume.ufrgs.br:10183/229520Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-09-19T07:31:16Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome
title Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome
spellingShingle Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome
Araujo, Gabriella Oliveira de
Enterococcus
Lycalopex gymnocercus
Felidae
Resistência microbiana a medicamentos
Fatores de virulência
Enterococcus spp.
Pampa biome
Wildlife animals
Pampas fox
Geoffroy’s cat
Multidrug-resistance
Virulence factors
Antibiotic resistance genes
title_short Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome
title_full Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome
title_fullStr Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome
title_full_unstemmed Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome
title_sort Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome
author Araujo, Gabriella Oliveira de
author_facet Araujo, Gabriella Oliveira de
Huff, Rosana
Favarini, Marina Ochoa
Mann, Michele Bertoni
Peters, Felipe Bortolotto
Frazzon, Jeverson
Frazzon, Ana Paula Guedes
author_role author
author2 Huff, Rosana
Favarini, Marina Ochoa
Mann, Michele Bertoni
Peters, Felipe Bortolotto
Frazzon, Jeverson
Frazzon, Ana Paula Guedes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araujo, Gabriella Oliveira de
Huff, Rosana
Favarini, Marina Ochoa
Mann, Michele Bertoni
Peters, Felipe Bortolotto
Frazzon, Jeverson
Frazzon, Ana Paula Guedes
dc.subject.por.fl_str_mv Enterococcus
Lycalopex gymnocercus
Felidae
Resistência microbiana a medicamentos
Fatores de virulência
topic Enterococcus
Lycalopex gymnocercus
Felidae
Resistência microbiana a medicamentos
Fatores de virulência
Enterococcus spp.
Pampa biome
Wildlife animals
Pampas fox
Geoffroy’s cat
Multidrug-resistance
Virulence factors
Antibiotic resistance genes
dc.subject.eng.fl_str_mv Enterococcus spp.
Pampa biome
Wildlife animals
Pampas fox
Geoffroy’s cat
Multidrug-resistance
Virulence factors
Antibiotic resistance genes
description Enterococci are ubiquitous microorganisms present in various environments and within the gastrointestinal tracts of humans and other animals. Notably, fecal enterococci are suitable indicators for monitoring antimicrobial resistance dissemination. Resistant bacterial strains recovered from the fecal samples of wild animals can highlight important aspects of environmental disturbances. In this report, we investigated antimicrobial susceptibility as well as resistance and virulence genes in fecal enterococci isolated from wild Pampas foxes (Lycalopex gymnocercus) (n = 5) and Geoffroy's cats (Leopardus geoffroyi) (n = 4) in the Brazilian Pampa biome. Enterococci were isolated from eight out of nine fecal samples and Enterococcus faecalis was identified in both animals. However, E. faecium and E. durans were only detected in Pampas foxes, while E. hirae was only detected in Geoffroy's cats. Antimicrobial susceptibility analysis showed resistance to rifampicin (94%), erythromycin (72.6%), ciprofloxacin/norfloxacin (40%), streptomycin (38%), and tetracycline (26%). The high frequency of multidrug-resistant enterococci (66%) isolated in this study is a matter of concern since these are wild animals with no history of therapeutic antibiotic exposure. The tetM/tetL and msrC/ermB genes were detected in most tetracycline- and erythromycin-resistant enterococci, respectively. The gelE, ace, agg, esp, and clyA virulence genes were also detected in enterococci. In conclusion, our data suggest that habitat fragmentation and anthropogenic activities in the Pampa biome may contribute to high frequencies of multidrug-resistant enterococci in the gut communities of wild Pampas foxes and Geoffroy's cats. To the best of the authors' knowledge, this is the first report of antimicrobial-resistant enterococci in the Pampa biome.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-09-03T04:25:43Z
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dc.relation.ispartof.pt_BR.fl_str_mv Frontiers in veterinary science. Lausanne. Vol. 7 (Dec. 2020), 606377, 10 p.
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