Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/229520 |
Resumo: | Enterococci are ubiquitous microorganisms present in various environments and within the gastrointestinal tracts of humans and other animals. Notably, fecal enterococci are suitable indicators for monitoring antimicrobial resistance dissemination. Resistant bacterial strains recovered from the fecal samples of wild animals can highlight important aspects of environmental disturbances. In this report, we investigated antimicrobial susceptibility as well as resistance and virulence genes in fecal enterococci isolated from wild Pampas foxes (Lycalopex gymnocercus) (n = 5) and Geoffroy's cats (Leopardus geoffroyi) (n = 4) in the Brazilian Pampa biome. Enterococci were isolated from eight out of nine fecal samples and Enterococcus faecalis was identified in both animals. However, E. faecium and E. durans were only detected in Pampas foxes, while E. hirae was only detected in Geoffroy's cats. Antimicrobial susceptibility analysis showed resistance to rifampicin (94%), erythromycin (72.6%), ciprofloxacin/norfloxacin (40%), streptomycin (38%), and tetracycline (26%). The high frequency of multidrug-resistant enterococci (66%) isolated in this study is a matter of concern since these are wild animals with no history of therapeutic antibiotic exposure. The tetM/tetL and msrC/ermB genes were detected in most tetracycline- and erythromycin-resistant enterococci, respectively. The gelE, ace, agg, esp, and clyA virulence genes were also detected in enterococci. In conclusion, our data suggest that habitat fragmentation and anthropogenic activities in the Pampa biome may contribute to high frequencies of multidrug-resistant enterococci in the gut communities of wild Pampas foxes and Geoffroy's cats. To the best of the authors' knowledge, this is the first report of antimicrobial-resistant enterococci in the Pampa biome. |
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Araujo, Gabriella Oliveira deHuff, RosanaFavarini, Marina OchoaMann, Michele BertoniPeters, Felipe BortolottoFrazzon, JeversonFrazzon, Ana Paula Guedes2021-09-03T04:25:43Z20202297-1769http://hdl.handle.net/10183/229520001130796Enterococci are ubiquitous microorganisms present in various environments and within the gastrointestinal tracts of humans and other animals. Notably, fecal enterococci are suitable indicators for monitoring antimicrobial resistance dissemination. Resistant bacterial strains recovered from the fecal samples of wild animals can highlight important aspects of environmental disturbances. In this report, we investigated antimicrobial susceptibility as well as resistance and virulence genes in fecal enterococci isolated from wild Pampas foxes (Lycalopex gymnocercus) (n = 5) and Geoffroy's cats (Leopardus geoffroyi) (n = 4) in the Brazilian Pampa biome. Enterococci were isolated from eight out of nine fecal samples and Enterococcus faecalis was identified in both animals. However, E. faecium and E. durans were only detected in Pampas foxes, while E. hirae was only detected in Geoffroy's cats. Antimicrobial susceptibility analysis showed resistance to rifampicin (94%), erythromycin (72.6%), ciprofloxacin/norfloxacin (40%), streptomycin (38%), and tetracycline (26%). The high frequency of multidrug-resistant enterococci (66%) isolated in this study is a matter of concern since these are wild animals with no history of therapeutic antibiotic exposure. The tetM/tetL and msrC/ermB genes were detected in most tetracycline- and erythromycin-resistant enterococci, respectively. The gelE, ace, agg, esp, and clyA virulence genes were also detected in enterococci. In conclusion, our data suggest that habitat fragmentation and anthropogenic activities in the Pampa biome may contribute to high frequencies of multidrug-resistant enterococci in the gut communities of wild Pampas foxes and Geoffroy's cats. To the best of the authors' knowledge, this is the first report of antimicrobial-resistant enterococci in the Pampa biome.application/pdfengFrontiers in veterinary science. Lausanne. Vol. 7 (Dec. 2020), 606377, 10 p.EnterococcusLycalopex gymnocercusFelidaeResistência microbiana a medicamentosFatores de virulênciaEnterococcus spp.Pampa biomeWildlife animalsPampas foxGeoffroy’s catMultidrug-resistanceVirulence factorsAntibiotic resistance genesMultidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biomeEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001130796.pdf.txt001130796.pdf.txtExtracted Texttext/plain53599http://www.lume.ufrgs.br/bitstream/10183/229520/2/001130796.pdf.txtced0e2512d0ee237df4f5505694dc2a4MD52ORIGINAL001130796.pdfTexto completo (inglês)application/pdf756515http://www.lume.ufrgs.br/bitstream/10183/229520/1/001130796.pdf81279f5965c8fb06d16f61f5c64f8332MD5110183/2295202021-09-19 04:31:16.856504oai:www.lume.ufrgs.br:10183/229520Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-09-19T07:31:16Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome |
title |
Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome |
spellingShingle |
Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome Araujo, Gabriella Oliveira de Enterococcus Lycalopex gymnocercus Felidae Resistência microbiana a medicamentos Fatores de virulência Enterococcus spp. Pampa biome Wildlife animals Pampas fox Geoffroy’s cat Multidrug-resistance Virulence factors Antibiotic resistance genes |
title_short |
Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome |
title_full |
Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome |
title_fullStr |
Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome |
title_full_unstemmed |
Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome |
title_sort |
Multidrug resistance in Enterococci isolated from wild pampas foxes (Lycalopex gymnocercus) and Geoffroy's Cats (Leopardus geoffroyi) in the Brazilian pampa biome |
author |
Araujo, Gabriella Oliveira de |
author_facet |
Araujo, Gabriella Oliveira de Huff, Rosana Favarini, Marina Ochoa Mann, Michele Bertoni Peters, Felipe Bortolotto Frazzon, Jeverson Frazzon, Ana Paula Guedes |
author_role |
author |
author2 |
Huff, Rosana Favarini, Marina Ochoa Mann, Michele Bertoni Peters, Felipe Bortolotto Frazzon, Jeverson Frazzon, Ana Paula Guedes |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Araujo, Gabriella Oliveira de Huff, Rosana Favarini, Marina Ochoa Mann, Michele Bertoni Peters, Felipe Bortolotto Frazzon, Jeverson Frazzon, Ana Paula Guedes |
dc.subject.por.fl_str_mv |
Enterococcus Lycalopex gymnocercus Felidae Resistência microbiana a medicamentos Fatores de virulência |
topic |
Enterococcus Lycalopex gymnocercus Felidae Resistência microbiana a medicamentos Fatores de virulência Enterococcus spp. Pampa biome Wildlife animals Pampas fox Geoffroy’s cat Multidrug-resistance Virulence factors Antibiotic resistance genes |
dc.subject.eng.fl_str_mv |
Enterococcus spp. Pampa biome Wildlife animals Pampas fox Geoffroy’s cat Multidrug-resistance Virulence factors Antibiotic resistance genes |
description |
Enterococci are ubiquitous microorganisms present in various environments and within the gastrointestinal tracts of humans and other animals. Notably, fecal enterococci are suitable indicators for monitoring antimicrobial resistance dissemination. Resistant bacterial strains recovered from the fecal samples of wild animals can highlight important aspects of environmental disturbances. In this report, we investigated antimicrobial susceptibility as well as resistance and virulence genes in fecal enterococci isolated from wild Pampas foxes (Lycalopex gymnocercus) (n = 5) and Geoffroy's cats (Leopardus geoffroyi) (n = 4) in the Brazilian Pampa biome. Enterococci were isolated from eight out of nine fecal samples and Enterococcus faecalis was identified in both animals. However, E. faecium and E. durans were only detected in Pampas foxes, while E. hirae was only detected in Geoffroy's cats. Antimicrobial susceptibility analysis showed resistance to rifampicin (94%), erythromycin (72.6%), ciprofloxacin/norfloxacin (40%), streptomycin (38%), and tetracycline (26%). The high frequency of multidrug-resistant enterococci (66%) isolated in this study is a matter of concern since these are wild animals with no history of therapeutic antibiotic exposure. The tetM/tetL and msrC/ermB genes were detected in most tetracycline- and erythromycin-resistant enterococci, respectively. The gelE, ace, agg, esp, and clyA virulence genes were also detected in enterococci. In conclusion, our data suggest that habitat fragmentation and anthropogenic activities in the Pampa biome may contribute to high frequencies of multidrug-resistant enterococci in the gut communities of wild Pampas foxes and Geoffroy's cats. To the best of the authors' knowledge, this is the first report of antimicrobial-resistant enterococci in the Pampa biome. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2021-09-03T04:25:43Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
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http://hdl.handle.net/10183/229520 |
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2297-1769 |
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001130796 |
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http://hdl.handle.net/10183/229520 |
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Frontiers in veterinary science. Lausanne. Vol. 7 (Dec. 2020), 606377, 10 p. |
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