Effects of vitamin D administration on nociception and spinal cord pro-oxidant and antioxidant markers in a rat model of neuropathic pain
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/230520 |
Resumo: | Reactive oxygen species (ROS) are involved in neuropathic pain, a complicated condition after nerve tissue lesion. Vitamin D appears to improve symptoms of pain and exhibits antioxidant properties. We investigated the effects of oral administration of vitamin D3, the active form of vitamin D, on nociception, the sciatic functional index (SFI), and spinal cord pro-oxidant and antioxidant markers in rats with chronic constriction injury (CCI) of the sciatic nerve, a model of neuropathic pain. Vitamin D3 (500 IU/kg per day) attenuated the CCI-induced decrease in mechanical withdrawal threshold and thermal withdrawal latency (indicators of antinociception) and SFI. The vitamin prevented increased lipid hydroperoxide levels in injured sciatic nerve without change to total antioxidant capacity (TAC). Vitamin D3 prevented increased lipid hydroperoxide, superoxide anion generation (SAG), and hydrogen peroxide (H2O2) levels in the spinal cord, which were found in rats without treatment at 7 and 28 days post-CCI. A significant negative correlation was found between mechanical threshold and SAG and between mechanical threshold and H2O2 at day 7. Vitamin D3 also prevented decreased spinal cord total thiols content. There was an increase in TAC in the spinal cord of vitamin-treated CCI rats, compared to CCI rats without treatment only at 28 days. No significant changes were found in body weight and blood parameters of hepatic and renal function. These findings demonstrated, for first time, that vitamin D modulated pro-oxidant and antioxidant markers in the spinal cord. Since antinociception occurred in parallel with oxidative changes in the spinal cord, the oxidative changes may have contributed to vitamin D-induced antinociception. |
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Santos, Maria do Carmo QuevedoSilva, Thaisla Cristiane Borella daSilva, Francielle Braz Oliveira daSiebert, CassianaKroth, AdarlySilveira, Elza Maria Santos daWyse, Angela Terezinha de SouzaPartata, Wania Aparecida2021-10-06T04:13:27Z20210100-879Xhttp://hdl.handle.net/10183/230520001131246Reactive oxygen species (ROS) are involved in neuropathic pain, a complicated condition after nerve tissue lesion. Vitamin D appears to improve symptoms of pain and exhibits antioxidant properties. We investigated the effects of oral administration of vitamin D3, the active form of vitamin D, on nociception, the sciatic functional index (SFI), and spinal cord pro-oxidant and antioxidant markers in rats with chronic constriction injury (CCI) of the sciatic nerve, a model of neuropathic pain. Vitamin D3 (500 IU/kg per day) attenuated the CCI-induced decrease in mechanical withdrawal threshold and thermal withdrawal latency (indicators of antinociception) and SFI. The vitamin prevented increased lipid hydroperoxide levels in injured sciatic nerve without change to total antioxidant capacity (TAC). Vitamin D3 prevented increased lipid hydroperoxide, superoxide anion generation (SAG), and hydrogen peroxide (H2O2) levels in the spinal cord, which were found in rats without treatment at 7 and 28 days post-CCI. A significant negative correlation was found between mechanical threshold and SAG and between mechanical threshold and H2O2 at day 7. Vitamin D3 also prevented decreased spinal cord total thiols content. There was an increase in TAC in the spinal cord of vitamin-treated CCI rats, compared to CCI rats without treatment only at 28 days. No significant changes were found in body weight and blood parameters of hepatic and renal function. These findings demonstrated, for first time, that vitamin D modulated pro-oxidant and antioxidant markers in the spinal cord. Since antinociception occurred in parallel with oxidative changes in the spinal cord, the oxidative changes may have contributed to vitamin D-induced antinociception.application/pdfengBrazilian journal of medical and biological research. Ribeirão Preto. Vol. 54, no. 10 (2021), e11207, 11 p.Vitamina DNeuralgiaColecalciferolNervo isquiáticoAntioxidantesEspécies reativas de oxigênioTotal thiol contentSuperoxide anion generationTotal antioxidant capacitySciatic functional indexMechanical withdrawal thresholdThermal withdrawal latencyEffects of vitamin D administration on nociception and spinal cord pro-oxidant and antioxidant markers in a rat model of neuropathic paininfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001131246.pdf.txt001131246.pdf.txtExtracted Texttext/plain48724http://www.lume.ufrgs.br/bitstream/10183/230520/2/001131246.pdf.txt8bfeb808af057448c922b030c43dc805MD52ORIGINAL001131246.pdfTexto completo (inglês)application/pdf728418http://www.lume.ufrgs.br/bitstream/10183/230520/1/001131246.pdf6636a1f0e4654a6a92ae8637b53580ceMD5110183/2305202021-11-20 06:02:05.273559oai:www.lume.ufrgs.br:10183/230520Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T08:02:05Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Effects of vitamin D administration on nociception and spinal cord pro-oxidant and antioxidant markers in a rat model of neuropathic pain |
title |
Effects of vitamin D administration on nociception and spinal cord pro-oxidant and antioxidant markers in a rat model of neuropathic pain |
spellingShingle |
Effects of vitamin D administration on nociception and spinal cord pro-oxidant and antioxidant markers in a rat model of neuropathic pain Santos, Maria do Carmo Quevedo Vitamina D Neuralgia Colecalciferol Nervo isquiático Antioxidantes Espécies reativas de oxigênio Total thiol content Superoxide anion generation Total antioxidant capacity Sciatic functional index Mechanical withdrawal threshold Thermal withdrawal latency |
title_short |
Effects of vitamin D administration on nociception and spinal cord pro-oxidant and antioxidant markers in a rat model of neuropathic pain |
title_full |
Effects of vitamin D administration on nociception and spinal cord pro-oxidant and antioxidant markers in a rat model of neuropathic pain |
title_fullStr |
Effects of vitamin D administration on nociception and spinal cord pro-oxidant and antioxidant markers in a rat model of neuropathic pain |
title_full_unstemmed |
Effects of vitamin D administration on nociception and spinal cord pro-oxidant and antioxidant markers in a rat model of neuropathic pain |
title_sort |
Effects of vitamin D administration on nociception and spinal cord pro-oxidant and antioxidant markers in a rat model of neuropathic pain |
author |
Santos, Maria do Carmo Quevedo |
author_facet |
Santos, Maria do Carmo Quevedo Silva, Thaisla Cristiane Borella da Silva, Francielle Braz Oliveira da Siebert, Cassiana Kroth, Adarly Silveira, Elza Maria Santos da Wyse, Angela Terezinha de Souza Partata, Wania Aparecida |
author_role |
author |
author2 |
Silva, Thaisla Cristiane Borella da Silva, Francielle Braz Oliveira da Siebert, Cassiana Kroth, Adarly Silveira, Elza Maria Santos da Wyse, Angela Terezinha de Souza Partata, Wania Aparecida |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Maria do Carmo Quevedo Silva, Thaisla Cristiane Borella da Silva, Francielle Braz Oliveira da Siebert, Cassiana Kroth, Adarly Silveira, Elza Maria Santos da Wyse, Angela Terezinha de Souza Partata, Wania Aparecida |
dc.subject.por.fl_str_mv |
Vitamina D Neuralgia Colecalciferol Nervo isquiático Antioxidantes Espécies reativas de oxigênio |
topic |
Vitamina D Neuralgia Colecalciferol Nervo isquiático Antioxidantes Espécies reativas de oxigênio Total thiol content Superoxide anion generation Total antioxidant capacity Sciatic functional index Mechanical withdrawal threshold Thermal withdrawal latency |
dc.subject.eng.fl_str_mv |
Total thiol content Superoxide anion generation Total antioxidant capacity Sciatic functional index Mechanical withdrawal threshold Thermal withdrawal latency |
description |
Reactive oxygen species (ROS) are involved in neuropathic pain, a complicated condition after nerve tissue lesion. Vitamin D appears to improve symptoms of pain and exhibits antioxidant properties. We investigated the effects of oral administration of vitamin D3, the active form of vitamin D, on nociception, the sciatic functional index (SFI), and spinal cord pro-oxidant and antioxidant markers in rats with chronic constriction injury (CCI) of the sciatic nerve, a model of neuropathic pain. Vitamin D3 (500 IU/kg per day) attenuated the CCI-induced decrease in mechanical withdrawal threshold and thermal withdrawal latency (indicators of antinociception) and SFI. The vitamin prevented increased lipid hydroperoxide levels in injured sciatic nerve without change to total antioxidant capacity (TAC). Vitamin D3 prevented increased lipid hydroperoxide, superoxide anion generation (SAG), and hydrogen peroxide (H2O2) levels in the spinal cord, which were found in rats without treatment at 7 and 28 days post-CCI. A significant negative correlation was found between mechanical threshold and SAG and between mechanical threshold and H2O2 at day 7. Vitamin D3 also prevented decreased spinal cord total thiols content. There was an increase in TAC in the spinal cord of vitamin-treated CCI rats, compared to CCI rats without treatment only at 28 days. No significant changes were found in body weight and blood parameters of hepatic and renal function. These findings demonstrated, for first time, that vitamin D modulated pro-oxidant and antioxidant markers in the spinal cord. Since antinociception occurred in parallel with oxidative changes in the spinal cord, the oxidative changes may have contributed to vitamin D-induced antinociception. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-10-06T04:13:27Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/230520 |
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0100-879X |
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001131246 |
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http://hdl.handle.net/10183/230520 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research. Ribeirão Preto. Vol. 54, no. 10 (2021), e11207, 11 p. |
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info:eu-repo/semantics/openAccess |
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