Sida tuberculata (Malvaceae) : a study based on development of extractive system and in silico and in vitro properties
Main Author: | |
---|---|
Publication Date: | 2016 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UFRGS |
Download full: | http://hdl.handle.net/10183/230209 |
Summary: | Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculata extracts using in silico and in vitro models. Therefore, in order to determine the dry residue content and the main compound 20-hydroxyecdysone (20E) concentration, extracts from leaves and roots were prepared testing ethanol and water in different proportions. Extracts were then assessed by Artemia salina lethality test, and toxicity prediction of 20E was estimated. Antioxidant activity was performed by DPPH and ABTS radical scavenger assays, ferric reducing power assay, nitrogen derivative scavenger, deoxyribose degradation, and TBARS assays. HPLC evaluation detected 20E as main compound in leaves and roots. Percolation method showed the highest concentrations of 20E (0.134 and 0.096 mg/mL of extract for leaves and roots, respectively). All crude extracts presented low toxic potential on A. salina (LD50 41000 mg/mL). The computational evaluation of 20E showed a low toxicity prediction. For in vitro antioxidant tests, hydroethanolic extracts of leaves were most effective compared to roots. In addition, hydroethanolic extracts presented a higher IC50 antioxidant than aqueous extracts. TBARS formation was prevented by leaves hydroethanolic extract from 0.015 and 0.03 mg/mL and for roots from 0.03 and 0.3 mg/mL on egg yolk and rat tissue, respectively (Po0.05). These findings suggest that S. tuberculata extracts are a considerable source of ecdysteroids and possesses a significant antioxidant property with low toxic potential. |
id |
UFRGS-2_4fde86f8b302a980c85e5ccb1bd893d5 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/230209 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Rosa, Hemerson Silva daSalgueiro, Andréia Caroline FernandesOliveira, Ana Zilda Ceolin Colpo Simões dePaula, Fávero ReisdorferMendez, Andreas Sebastian LoureiroFolmer, Vanderlei2021-09-24T04:22:44Z20160100-879Xhttp://hdl.handle.net/10183/230209000998406Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculata extracts using in silico and in vitro models. Therefore, in order to determine the dry residue content and the main compound 20-hydroxyecdysone (20E) concentration, extracts from leaves and roots were prepared testing ethanol and water in different proportions. Extracts were then assessed by Artemia salina lethality test, and toxicity prediction of 20E was estimated. Antioxidant activity was performed by DPPH and ABTS radical scavenger assays, ferric reducing power assay, nitrogen derivative scavenger, deoxyribose degradation, and TBARS assays. HPLC evaluation detected 20E as main compound in leaves and roots. Percolation method showed the highest concentrations of 20E (0.134 and 0.096 mg/mL of extract for leaves and roots, respectively). All crude extracts presented low toxic potential on A. salina (LD50 41000 mg/mL). The computational evaluation of 20E showed a low toxicity prediction. For in vitro antioxidant tests, hydroethanolic extracts of leaves were most effective compared to roots. In addition, hydroethanolic extracts presented a higher IC50 antioxidant than aqueous extracts. TBARS formation was prevented by leaves hydroethanolic extract from 0.015 and 0.03 mg/mL and for roots from 0.03 and 0.3 mg/mL on egg yolk and rat tissue, respectively (Po0.05). These findings suggest that S. tuberculata extracts are a considerable source of ecdysteroids and possesses a significant antioxidant property with low toxic potential.application/pdfengBrazilian journal of medical and biological research. Vol. 49, n. 8 (2016), p. 1-10AntifúngicosFarmáciaMalvaceaeSida tuberculata20-hydroxyecdysoneToxicity predictionAntioxidantSida tuberculata (Malvaceae) : a study based on development of extractive system and in silico and in vitro propertiesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000998406.pdf.txt000998406.pdf.txtExtracted Texttext/plain43872http://www.lume.ufrgs.br/bitstream/10183/230209/2/000998406.pdf.txtef7010db1ffa0b6023b2b82cf3411784MD52ORIGINAL000998406.pdfTexto completo (inglês)application/pdf781282http://www.lume.ufrgs.br/bitstream/10183/230209/1/000998406.pdff2645ef6ab7b432cf0e9927e0fcfeb44MD5110183/2302092021-10-04 04:25:04.466814oai:www.lume.ufrgs.br:10183/230209Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-10-04T07:25:04Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Sida tuberculata (Malvaceae) : a study based on development of extractive system and in silico and in vitro properties |
title |
Sida tuberculata (Malvaceae) : a study based on development of extractive system and in silico and in vitro properties |
spellingShingle |
Sida tuberculata (Malvaceae) : a study based on development of extractive system and in silico and in vitro properties Rosa, Hemerson Silva da Antifúngicos Farmácia Malvaceae Sida tuberculata 20-hydroxyecdysone Toxicity prediction Antioxidant |
title_short |
Sida tuberculata (Malvaceae) : a study based on development of extractive system and in silico and in vitro properties |
title_full |
Sida tuberculata (Malvaceae) : a study based on development of extractive system and in silico and in vitro properties |
title_fullStr |
Sida tuberculata (Malvaceae) : a study based on development of extractive system and in silico and in vitro properties |
title_full_unstemmed |
Sida tuberculata (Malvaceae) : a study based on development of extractive system and in silico and in vitro properties |
title_sort |
Sida tuberculata (Malvaceae) : a study based on development of extractive system and in silico and in vitro properties |
author |
Rosa, Hemerson Silva da |
author_facet |
Rosa, Hemerson Silva da Salgueiro, Andréia Caroline Fernandes Oliveira, Ana Zilda Ceolin Colpo Simões de Paula, Fávero Reisdorfer Mendez, Andreas Sebastian Loureiro Folmer, Vanderlei |
author_role |
author |
author2 |
Salgueiro, Andréia Caroline Fernandes Oliveira, Ana Zilda Ceolin Colpo Simões de Paula, Fávero Reisdorfer Mendez, Andreas Sebastian Loureiro Folmer, Vanderlei |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rosa, Hemerson Silva da Salgueiro, Andréia Caroline Fernandes Oliveira, Ana Zilda Ceolin Colpo Simões de Paula, Fávero Reisdorfer Mendez, Andreas Sebastian Loureiro Folmer, Vanderlei |
dc.subject.por.fl_str_mv |
Antifúngicos Farmácia Malvaceae |
topic |
Antifúngicos Farmácia Malvaceae Sida tuberculata 20-hydroxyecdysone Toxicity prediction Antioxidant |
dc.subject.eng.fl_str_mv |
Sida tuberculata 20-hydroxyecdysone Toxicity prediction Antioxidant |
description |
Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculata extracts using in silico and in vitro models. Therefore, in order to determine the dry residue content and the main compound 20-hydroxyecdysone (20E) concentration, extracts from leaves and roots were prepared testing ethanol and water in different proportions. Extracts were then assessed by Artemia salina lethality test, and toxicity prediction of 20E was estimated. Antioxidant activity was performed by DPPH and ABTS radical scavenger assays, ferric reducing power assay, nitrogen derivative scavenger, deoxyribose degradation, and TBARS assays. HPLC evaluation detected 20E as main compound in leaves and roots. Percolation method showed the highest concentrations of 20E (0.134 and 0.096 mg/mL of extract for leaves and roots, respectively). All crude extracts presented low toxic potential on A. salina (LD50 41000 mg/mL). The computational evaluation of 20E showed a low toxicity prediction. For in vitro antioxidant tests, hydroethanolic extracts of leaves were most effective compared to roots. In addition, hydroethanolic extracts presented a higher IC50 antioxidant than aqueous extracts. TBARS formation was prevented by leaves hydroethanolic extract from 0.015 and 0.03 mg/mL and for roots from 0.03 and 0.3 mg/mL on egg yolk and rat tissue, respectively (Po0.05). These findings suggest that S. tuberculata extracts are a considerable source of ecdysteroids and possesses a significant antioxidant property with low toxic potential. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2021-09-24T04:22:44Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/230209 |
dc.identifier.issn.pt_BR.fl_str_mv |
0100-879X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000998406 |
identifier_str_mv |
0100-879X 000998406 |
url |
http://hdl.handle.net/10183/230209 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research. Vol. 49, n. 8 (2016), p. 1-10 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/230209/2/000998406.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/230209/1/000998406.pdf |
bitstream.checksum.fl_str_mv |
ef7010db1ffa0b6023b2b82cf3411784 f2645ef6ab7b432cf0e9927e0fcfeb44 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801225037270745088 |