Pathological findings of post-anesthetic myopathy associated with type 1 polysaccharide storage myopathy in a Percheron horse

Detalhes bibliográficos
Autor(a) principal: Fredo, Gabriela
Data de Publicação: 2018
Outros Autores: Bassuino, Daniele Mariath, Bianchi, Matheus Viezzer, Delfiol, Diego J.Z., Borges, Alexandre Secorun, Pavarini, Saulo Petinatti, Sonne, Luciana, Driemeier, David
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/188313
Resumo: Background: Post-anesthetic myopathy is the most common complication associated with general anesthesia in horses. Polysaccharide storage myopathy (PSSM) is characterized by an abnormal accumulation of glycogen and glycogen-related polysaccharides in the skeletal muscle, which is categorized in type 1 (PSSM1) and type 2 (PSSM2). The purpose of this study is to report the clinical, pathological and molecular findings in a Percheron mare with post-anesthetic myopathy associated with a PSSM1. Case: A 9-year-old Percheron mare was submitted to a caesarean section due to clinical dystocia during labor. Xylazine was employed during pre-anesthesia, followed by induction with ketamine and diazepam, while anesthetic maintenance was obtained with isoflurane. The mare showed good recovery, however 24 h later, sternal recumbency and hyperthermia (41° C) were observed. The mare was euthanized, and a necropsy was performed. Samples of multiple tissues were collected and routinely processed for histology. At necropsy, segments of skeletal muscles had bilateral pale areas. The kidneys had old and recent infarcts. The heart had whitish areas in the myocardium. The brain showed focally extensive reddish areas, with flattening of gyri. Histologically, skeletal muscle fibers had in the sarcoplasm multiple homogeneous globular clear eosinophilic formations, in addition to mild hyaline necrosis. In the heart and in the kidney, there were extensive areas of acute coagulative necrosis. The brain showed marked multifocal fibrinoid degeneration of vessels and hemorrhage. Refrigerated liver samples were submitted to DNA extraction to detect mutations in the GYS1 (type 1 PSSM) and RyR1 genes (malignant hyperthermia). A positive result for a homozygous dominant mutation in GYS1 (type 1 PSSM) was observed, while the mutation responsible for malignant hyperthermia was not identified Discussion: The diagnosis of post-anesthetic myopathy associated with PSSM was obtained by the presence of amylase resistant polysaccharide complex inclusions, glycogen subsarcolemmal aggregates, and central cytoplasmic corpuscles containing glycogen through PAS-amylase resistant histochemical technique, associated to the myopathy microscopical features. Microscopic findings were related to clinical history, and the diagnosis of PSSM underlying post-anesthetic myopathy was determined. The predisposition of the Percheron horse has been described as an inherited predisposition leading to PSSM susceptibility, as was observed in the present case. We speculated that the anesthetic procedure resulted in the precipitation of the drug and a presentation of an acute anesthetic myopathy, while the muscle damage most likely occurred due to the ischemia caused by systemic hypotension. In addition to these lesions, other lesions were considered related to the use of the anesthetics, which may predispose to vasculogenic injuries. This horse was diagnosed as being homozygous dominant for the GYS1 gene, which causes a gain-offunction and results in glycogenolysis with glycogen accumulation in myofibers. Horses that are homozygous for the GYS1 gene may exhibit more severe histological changes in the skeletal muscle fibers, such as necrosis, anisocytosis, endomysial fibrosis, and fatty infiltration In PSSM, there is a bilateral involvement of the skeletal muscles with areas of degeneration of whitish or greyish coloration, as well as pale muscle with whitish streaks due to coagulative necrosis and edema. In our study, we observed bilateral skeletal muscle lesions and cardiomyocyte necrosis. Post-anesthetic myopathy, along with skeletal muscle lesions, may predispose to vasculogenic injuries, with kidney and brain lesions in horses. Dominant homozygosis for the GYS1 gene with consequent PSSM1 disease probably aggravated the condition in this Percheron, with more severe histological muscular lesions. Our study should bring attention to the use of anesthetics in horses with PSSM1, especially in the Percheron breed.
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spelling Fredo, GabrielaBassuino, Daniele MariathBianchi, Matheus ViezzerDelfiol, Diego J.Z.Borges, Alexandre SecorunPavarini, Saulo PetinattiSonne, LucianaDriemeier, David2019-01-30T02:33:03Z20181678-0345http://hdl.handle.net/10183/188313001083807Background: Post-anesthetic myopathy is the most common complication associated with general anesthesia in horses. Polysaccharide storage myopathy (PSSM) is characterized by an abnormal accumulation of glycogen and glycogen-related polysaccharides in the skeletal muscle, which is categorized in type 1 (PSSM1) and type 2 (PSSM2). The purpose of this study is to report the clinical, pathological and molecular findings in a Percheron mare with post-anesthetic myopathy associated with a PSSM1. Case: A 9-year-old Percheron mare was submitted to a caesarean section due to clinical dystocia during labor. Xylazine was employed during pre-anesthesia, followed by induction with ketamine and diazepam, while anesthetic maintenance was obtained with isoflurane. The mare showed good recovery, however 24 h later, sternal recumbency and hyperthermia (41° C) were observed. The mare was euthanized, and a necropsy was performed. Samples of multiple tissues were collected and routinely processed for histology. At necropsy, segments of skeletal muscles had bilateral pale areas. The kidneys had old and recent infarcts. The heart had whitish areas in the myocardium. The brain showed focally extensive reddish areas, with flattening of gyri. Histologically, skeletal muscle fibers had in the sarcoplasm multiple homogeneous globular clear eosinophilic formations, in addition to mild hyaline necrosis. In the heart and in the kidney, there were extensive areas of acute coagulative necrosis. The brain showed marked multifocal fibrinoid degeneration of vessels and hemorrhage. Refrigerated liver samples were submitted to DNA extraction to detect mutations in the GYS1 (type 1 PSSM) and RyR1 genes (malignant hyperthermia). A positive result for a homozygous dominant mutation in GYS1 (type 1 PSSM) was observed, while the mutation responsible for malignant hyperthermia was not identified Discussion: The diagnosis of post-anesthetic myopathy associated with PSSM was obtained by the presence of amylase resistant polysaccharide complex inclusions, glycogen subsarcolemmal aggregates, and central cytoplasmic corpuscles containing glycogen through PAS-amylase resistant histochemical technique, associated to the myopathy microscopical features. Microscopic findings were related to clinical history, and the diagnosis of PSSM underlying post-anesthetic myopathy was determined. The predisposition of the Percheron horse has been described as an inherited predisposition leading to PSSM susceptibility, as was observed in the present case. We speculated that the anesthetic procedure resulted in the precipitation of the drug and a presentation of an acute anesthetic myopathy, while the muscle damage most likely occurred due to the ischemia caused by systemic hypotension. In addition to these lesions, other lesions were considered related to the use of the anesthetics, which may predispose to vasculogenic injuries. This horse was diagnosed as being homozygous dominant for the GYS1 gene, which causes a gain-offunction and results in glycogenolysis with glycogen accumulation in myofibers. Horses that are homozygous for the GYS1 gene may exhibit more severe histological changes in the skeletal muscle fibers, such as necrosis, anisocytosis, endomysial fibrosis, and fatty infiltration In PSSM, there is a bilateral involvement of the skeletal muscles with areas of degeneration of whitish or greyish coloration, as well as pale muscle with whitish streaks due to coagulative necrosis and edema. In our study, we observed bilateral skeletal muscle lesions and cardiomyocyte necrosis. Post-anesthetic myopathy, along with skeletal muscle lesions, may predispose to vasculogenic injuries, with kidney and brain lesions in horses. Dominant homozygosis for the GYS1 gene with consequent PSSM1 disease probably aggravated the condition in this Percheron, with more severe histological muscular lesions. Our study should bring attention to the use of anesthetics in horses with PSSM1, especially in the Percheron breed.application/pdfengActa scientiae veterinariae. Porto Alegre, RS. Vol. 46, supl. 1 (2018), Pub. 346, 6 p.Miopatia pós-anestésicaMúsculo esqueléticoPolissacarídeosEquinosHorsesMetabolic diseasePSSMPeriodic acid-schiffSkeletal muscle diseasesPathological findings of post-anesthetic myopathy associated with type 1 polysaccharide storage myopathy in a Percheron horseinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001083807.pdf.txt001083807.pdf.txtExtracted Texttext/plain22855http://www.lume.ufrgs.br/bitstream/10183/188313/2/001083807.pdf.txtb017cd4c65279f7d40d4ed5f76cce9e5MD52ORIGINAL001083807.pdfTexto completo (inglês)application/pdf2847144http://www.lume.ufrgs.br/bitstream/10183/188313/1/001083807.pdf7df1b1e00aae496e61092be0e41e7cadMD5110183/1883132019-01-31 02:32:41.412562oai:www.lume.ufrgs.br:10183/188313Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-01-31T04:32:41Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Pathological findings of post-anesthetic myopathy associated with type 1 polysaccharide storage myopathy in a Percheron horse
title Pathological findings of post-anesthetic myopathy associated with type 1 polysaccharide storage myopathy in a Percheron horse
spellingShingle Pathological findings of post-anesthetic myopathy associated with type 1 polysaccharide storage myopathy in a Percheron horse
Fredo, Gabriela
Miopatia pós-anestésica
Músculo esquelético
Polissacarídeos
Equinos
Horses
Metabolic disease
PSSM
Periodic acid-schiff
Skeletal muscle diseases
title_short Pathological findings of post-anesthetic myopathy associated with type 1 polysaccharide storage myopathy in a Percheron horse
title_full Pathological findings of post-anesthetic myopathy associated with type 1 polysaccharide storage myopathy in a Percheron horse
title_fullStr Pathological findings of post-anesthetic myopathy associated with type 1 polysaccharide storage myopathy in a Percheron horse
title_full_unstemmed Pathological findings of post-anesthetic myopathy associated with type 1 polysaccharide storage myopathy in a Percheron horse
title_sort Pathological findings of post-anesthetic myopathy associated with type 1 polysaccharide storage myopathy in a Percheron horse
author Fredo, Gabriela
author_facet Fredo, Gabriela
Bassuino, Daniele Mariath
Bianchi, Matheus Viezzer
Delfiol, Diego J.Z.
Borges, Alexandre Secorun
Pavarini, Saulo Petinatti
Sonne, Luciana
Driemeier, David
author_role author
author2 Bassuino, Daniele Mariath
Bianchi, Matheus Viezzer
Delfiol, Diego J.Z.
Borges, Alexandre Secorun
Pavarini, Saulo Petinatti
Sonne, Luciana
Driemeier, David
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fredo, Gabriela
Bassuino, Daniele Mariath
Bianchi, Matheus Viezzer
Delfiol, Diego J.Z.
Borges, Alexandre Secorun
Pavarini, Saulo Petinatti
Sonne, Luciana
Driemeier, David
dc.subject.por.fl_str_mv Miopatia pós-anestésica
Músculo esquelético
Polissacarídeos
Equinos
topic Miopatia pós-anestésica
Músculo esquelético
Polissacarídeos
Equinos
Horses
Metabolic disease
PSSM
Periodic acid-schiff
Skeletal muscle diseases
dc.subject.eng.fl_str_mv Horses
Metabolic disease
PSSM
Periodic acid-schiff
Skeletal muscle diseases
description Background: Post-anesthetic myopathy is the most common complication associated with general anesthesia in horses. Polysaccharide storage myopathy (PSSM) is characterized by an abnormal accumulation of glycogen and glycogen-related polysaccharides in the skeletal muscle, which is categorized in type 1 (PSSM1) and type 2 (PSSM2). The purpose of this study is to report the clinical, pathological and molecular findings in a Percheron mare with post-anesthetic myopathy associated with a PSSM1. Case: A 9-year-old Percheron mare was submitted to a caesarean section due to clinical dystocia during labor. Xylazine was employed during pre-anesthesia, followed by induction with ketamine and diazepam, while anesthetic maintenance was obtained with isoflurane. The mare showed good recovery, however 24 h later, sternal recumbency and hyperthermia (41° C) were observed. The mare was euthanized, and a necropsy was performed. Samples of multiple tissues were collected and routinely processed for histology. At necropsy, segments of skeletal muscles had bilateral pale areas. The kidneys had old and recent infarcts. The heart had whitish areas in the myocardium. The brain showed focally extensive reddish areas, with flattening of gyri. Histologically, skeletal muscle fibers had in the sarcoplasm multiple homogeneous globular clear eosinophilic formations, in addition to mild hyaline necrosis. In the heart and in the kidney, there were extensive areas of acute coagulative necrosis. The brain showed marked multifocal fibrinoid degeneration of vessels and hemorrhage. Refrigerated liver samples were submitted to DNA extraction to detect mutations in the GYS1 (type 1 PSSM) and RyR1 genes (malignant hyperthermia). A positive result for a homozygous dominant mutation in GYS1 (type 1 PSSM) was observed, while the mutation responsible for malignant hyperthermia was not identified Discussion: The diagnosis of post-anesthetic myopathy associated with PSSM was obtained by the presence of amylase resistant polysaccharide complex inclusions, glycogen subsarcolemmal aggregates, and central cytoplasmic corpuscles containing glycogen through PAS-amylase resistant histochemical technique, associated to the myopathy microscopical features. Microscopic findings were related to clinical history, and the diagnosis of PSSM underlying post-anesthetic myopathy was determined. The predisposition of the Percheron horse has been described as an inherited predisposition leading to PSSM susceptibility, as was observed in the present case. We speculated that the anesthetic procedure resulted in the precipitation of the drug and a presentation of an acute anesthetic myopathy, while the muscle damage most likely occurred due to the ischemia caused by systemic hypotension. In addition to these lesions, other lesions were considered related to the use of the anesthetics, which may predispose to vasculogenic injuries. This horse was diagnosed as being homozygous dominant for the GYS1 gene, which causes a gain-offunction and results in glycogenolysis with glycogen accumulation in myofibers. Horses that are homozygous for the GYS1 gene may exhibit more severe histological changes in the skeletal muscle fibers, such as necrosis, anisocytosis, endomysial fibrosis, and fatty infiltration In PSSM, there is a bilateral involvement of the skeletal muscles with areas of degeneration of whitish or greyish coloration, as well as pale muscle with whitish streaks due to coagulative necrosis and edema. In our study, we observed bilateral skeletal muscle lesions and cardiomyocyte necrosis. Post-anesthetic myopathy, along with skeletal muscle lesions, may predispose to vasculogenic injuries, with kidney and brain lesions in horses. Dominant homozygosis for the GYS1 gene with consequent PSSM1 disease probably aggravated the condition in this Percheron, with more severe histological muscular lesions. Our study should bring attention to the use of anesthetics in horses with PSSM1, especially in the Percheron breed.
publishDate 2018
dc.date.issued.fl_str_mv 2018
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dc.relation.ispartof.pt_BR.fl_str_mv Acta scientiae veterinariae. Porto Alegre, RS. Vol. 46, supl. 1 (2018), Pub. 346, 6 p.
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