Desenvolvimento e validação de ensaio microbiológico para determinação de ciprofloxacino em pomada oftálmica

Detalhes bibliográficos
Autor(a) principal: Santer, Andressa
Data de Publicação: 2017
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/217895
Resumo: Ciprofloxacin is a second generation fluoroquinolone antimicrobial with a wide spectrum of activity against Gram-positive and Gram-negative bacteria. This paper describes the development and validation of a sensitive, accurate and reproducible microbiological assay, apply agar diffusion method for the determination of ciprofloxacin ophtalmic ointment. The assay is based on the inhibitory effect of ciprofloxacin upon the strain of Staphylococcus epidermidis ATCC12228 used as the test microorganism. The results were treated statistically by analysis of variance (ANOVA) and were found to be linear (r = 0.9903) in the selected range of 4.0 – 32.0 μg mL-1, precise with relative standard deviation (RSD) of repeatability intraday = 0.27, intermediate precision RSD = 0.53, and accurate (101.38%). The results demonstrated the validity of the proposed bioassay, which allows reliable ciprofloxacin quantitation in pharmaceutical samples and therefore can be used for the drug analysis in routine quality control of ciprofloxacin.
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spelling Santer, AndressaOppe, Tercio PaschkeCordenonsi, Leticia Malgarim2021-02-11T04:10:34Z2017http://hdl.handle.net/10183/217895001065981Ciprofloxacin is a second generation fluoroquinolone antimicrobial with a wide spectrum of activity against Gram-positive and Gram-negative bacteria. This paper describes the development and validation of a sensitive, accurate and reproducible microbiological assay, apply agar diffusion method for the determination of ciprofloxacin ophtalmic ointment. The assay is based on the inhibitory effect of ciprofloxacin upon the strain of Staphylococcus epidermidis ATCC12228 used as the test microorganism. The results were treated statistically by analysis of variance (ANOVA) and were found to be linear (r = 0.9903) in the selected range of 4.0 – 32.0 μg mL-1, precise with relative standard deviation (RSD) of repeatability intraday = 0.27, intermediate precision RSD = 0.53, and accurate (101.38%). The results demonstrated the validity of the proposed bioassay, which allows reliable ciprofloxacin quantitation in pharmaceutical samples and therefore can be used for the drug analysis in routine quality control of ciprofloxacin.application/pdfporCiprofloxacinoCiprofloxacinValidationAgar diffusion methodMicrobiological assayDesenvolvimento e validação de ensaio microbiológico para determinação de ciprofloxacino em pomada oftálmicainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisUniversidade Federal do Rio Grande do SulFaculdade de FarmáciaPorto Alegre, BR-RS2017Farmáciagraduaçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001065981.pdf.txt001065981.pdf.txtExtracted Texttext/plain43758http://www.lume.ufrgs.br/bitstream/10183/217895/2/001065981.pdf.txt6aa700321f97776c5d39980bee6ab26dMD52ORIGINAL001065981.pdfTexto completoapplication/pdf6675033http://www.lume.ufrgs.br/bitstream/10183/217895/1/001065981.pdfbf1d7e2b228343309f44753f97b746baMD5110183/2178952021-03-09 04:31:27.74659oai:www.lume.ufrgs.br:10183/217895Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-03-09T07:31:27Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Desenvolvimento e validação de ensaio microbiológico para determinação de ciprofloxacino em pomada oftálmica
title Desenvolvimento e validação de ensaio microbiológico para determinação de ciprofloxacino em pomada oftálmica
spellingShingle Desenvolvimento e validação de ensaio microbiológico para determinação de ciprofloxacino em pomada oftálmica
Santer, Andressa
Ciprofloxacino
Ciprofloxacin
Validation
Agar diffusion method
Microbiological assay
title_short Desenvolvimento e validação de ensaio microbiológico para determinação de ciprofloxacino em pomada oftálmica
title_full Desenvolvimento e validação de ensaio microbiológico para determinação de ciprofloxacino em pomada oftálmica
title_fullStr Desenvolvimento e validação de ensaio microbiológico para determinação de ciprofloxacino em pomada oftálmica
title_full_unstemmed Desenvolvimento e validação de ensaio microbiológico para determinação de ciprofloxacino em pomada oftálmica
title_sort Desenvolvimento e validação de ensaio microbiológico para determinação de ciprofloxacino em pomada oftálmica
author Santer, Andressa
author_facet Santer, Andressa
author_role author
dc.contributor.author.fl_str_mv Santer, Andressa
dc.contributor.advisor1.fl_str_mv Oppe, Tercio Paschke
dc.contributor.advisor-co1.fl_str_mv Cordenonsi, Leticia Malgarim
contributor_str_mv Oppe, Tercio Paschke
Cordenonsi, Leticia Malgarim
dc.subject.por.fl_str_mv Ciprofloxacino
topic Ciprofloxacino
Ciprofloxacin
Validation
Agar diffusion method
Microbiological assay
dc.subject.eng.fl_str_mv Ciprofloxacin
Validation
Agar diffusion method
Microbiological assay
description Ciprofloxacin is a second generation fluoroquinolone antimicrobial with a wide spectrum of activity against Gram-positive and Gram-negative bacteria. This paper describes the development and validation of a sensitive, accurate and reproducible microbiological assay, apply agar diffusion method for the determination of ciprofloxacin ophtalmic ointment. The assay is based on the inhibitory effect of ciprofloxacin upon the strain of Staphylococcus epidermidis ATCC12228 used as the test microorganism. The results were treated statistically by analysis of variance (ANOVA) and were found to be linear (r = 0.9903) in the selected range of 4.0 – 32.0 μg mL-1, precise with relative standard deviation (RSD) of repeatability intraday = 0.27, intermediate precision RSD = 0.53, and accurate (101.38%). The results demonstrated the validity of the proposed bioassay, which allows reliable ciprofloxacin quantitation in pharmaceutical samples and therefore can be used for the drug analysis in routine quality control of ciprofloxacin.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2021-02-11T04:10:34Z
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