The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy

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Autor(a) principal: Haag, Karen Luisa
Data de Publicação: 2009
Outros Autores: Gottstein, Bruno, Ayala, Francisco J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/21615
Resumo: Echinococcosis is a worldwide zoonotic parasitic disease of humans and various herbivorous domestic animals (intermediate hosts) transmitted by the contact with wild and domestic carnivores (definitive hosts), mainly foxes and dogs. Recently, a vaccine was developed showing high levels of protection against one parasite haplotype (G1) of Echinococcus granulosus, and its potential efficacy against distinct parasite variants or species is still unclear. Interestingly, the EG95 vaccine antigen is a secreted glycosylphosphatydilinositol (GPI)-anchored protein containing a fibronectin type III domain, which is ubiquitous in modular proteins involved in cell adhesion. EG95 is highly expressed in oncospheres, the parasite life cycle stage which actively invades the intermediate hosts. After amplifying and sequencing the complete CDS of 57 Echinococcus isolates belonging to 7 distinct species, we uncovered a large amount of genetic variability, which may influence protein folding. Two positively selected sites are outside the vaccine epitopes, but are predicted to alter protein conformation. Moreover, phylogenetic analyses indicate that EG95 isoform evolution is convergent with regard to the number of beta-sheets and alpha-helices. We conclude that having a variety of EG95 isoforms is adaptive for Echinococcus parasites, in terms of their ability to invade different hosts, and we propose that a mixture of isoforms could possibly maximize vaccine efficacy.
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spelling Haag, Karen LuisaGottstein, BrunoAyala, Francisco J.2010-05-06T04:16:41Z20091932-6203http://hdl.handle.net/10183/21615000692673Echinococcosis is a worldwide zoonotic parasitic disease of humans and various herbivorous domestic animals (intermediate hosts) transmitted by the contact with wild and domestic carnivores (definitive hosts), mainly foxes and dogs. Recently, a vaccine was developed showing high levels of protection against one parasite haplotype (G1) of Echinococcus granulosus, and its potential efficacy against distinct parasite variants or species is still unclear. Interestingly, the EG95 vaccine antigen is a secreted glycosylphosphatydilinositol (GPI)-anchored protein containing a fibronectin type III domain, which is ubiquitous in modular proteins involved in cell adhesion. EG95 is highly expressed in oncospheres, the parasite life cycle stage which actively invades the intermediate hosts. After amplifying and sequencing the complete CDS of 57 Echinococcus isolates belonging to 7 distinct species, we uncovered a large amount of genetic variability, which may influence protein folding. Two positively selected sites are outside the vaccine epitopes, but are predicted to alter protein conformation. Moreover, phylogenetic analyses indicate that EG95 isoform evolution is convergent with regard to the number of beta-sheets and alpha-helices. We conclude that having a variety of EG95 isoforms is adaptive for Echinococcus parasites, in terms of their ability to invade different hosts, and we propose that a mixture of isoforms could possibly maximize vaccine efficacy.application/zipapplication/zipapplication/pdfengPlos One. San Francisco. Vol. 4, no. 4 (apr. 2009), e5362, 8 p.Echinococcus sppEchinococcus granulosusBiologia molecularThe EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacyEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000692673.pdf000692673.pdfTexto completo (inglês)application/pdf1110925http://www.lume.ufrgs.br/bitstream/10183/21615/1/000692673.pdff8f4867d71a303b70aa9995173549ed4MD51000692673.zip000692673.zipMaterial suplementarapplication/zip90177http://www.lume.ufrgs.br/bitstream/10183/21615/2/000692673.zipc1355c73d201ca9a52eb4db3e46435beMD52000692673-02.zip000692673-02.zipTrabalho completo zipadoapplication/zip1192521http://www.lume.ufrgs.br/bitstream/10183/21615/3/000692673-02.zip791444c24d6429f1036d51673bf5b86bMD53TEXT000692673.pdf.txt000692673.pdf.txtExtracted Texttext/plain40777http://www.lume.ufrgs.br/bitstream/10183/21615/4/000692673.pdf.txt2ba829661835cc031a62ccf309da961cMD54THUMBNAIL000692673.pdf.jpg000692673.pdf.jpgGenerated Thumbnailimage/jpeg2076http://www.lume.ufrgs.br/bitstream/10183/21615/5/000692673.pdf.jpg988553a7b2b0e069e4614753dc410344MD5510183/216152023-09-23 03:36:50.787163oai:www.lume.ufrgs.br:10183/21615Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-23T06:36:50Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy
title The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy
spellingShingle The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy
Haag, Karen Luisa
Echinococcus spp
Echinococcus granulosus
Biologia molecular
title_short The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy
title_full The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy
title_fullStr The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy
title_full_unstemmed The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy
title_sort The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy
author Haag, Karen Luisa
author_facet Haag, Karen Luisa
Gottstein, Bruno
Ayala, Francisco J.
author_role author
author2 Gottstein, Bruno
Ayala, Francisco J.
author2_role author
author
dc.contributor.author.fl_str_mv Haag, Karen Luisa
Gottstein, Bruno
Ayala, Francisco J.
dc.subject.por.fl_str_mv Echinococcus spp
Echinococcus granulosus
Biologia molecular
topic Echinococcus spp
Echinococcus granulosus
Biologia molecular
description Echinococcosis is a worldwide zoonotic parasitic disease of humans and various herbivorous domestic animals (intermediate hosts) transmitted by the contact with wild and domestic carnivores (definitive hosts), mainly foxes and dogs. Recently, a vaccine was developed showing high levels of protection against one parasite haplotype (G1) of Echinococcus granulosus, and its potential efficacy against distinct parasite variants or species is still unclear. Interestingly, the EG95 vaccine antigen is a secreted glycosylphosphatydilinositol (GPI)-anchored protein containing a fibronectin type III domain, which is ubiquitous in modular proteins involved in cell adhesion. EG95 is highly expressed in oncospheres, the parasite life cycle stage which actively invades the intermediate hosts. After amplifying and sequencing the complete CDS of 57 Echinococcus isolates belonging to 7 distinct species, we uncovered a large amount of genetic variability, which may influence protein folding. Two positively selected sites are outside the vaccine epitopes, but are predicted to alter protein conformation. Moreover, phylogenetic analyses indicate that EG95 isoform evolution is convergent with regard to the number of beta-sheets and alpha-helices. We conclude that having a variety of EG95 isoforms is adaptive for Echinococcus parasites, in terms of their ability to invade different hosts, and we propose that a mixture of isoforms could possibly maximize vaccine efficacy.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2010-05-06T04:16:41Z
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dc.relation.ispartof.pt_BR.fl_str_mv Plos One. San Francisco. Vol. 4, no. 4 (apr. 2009), e5362, 8 p.
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