The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/21615 |
Resumo: | Echinococcosis is a worldwide zoonotic parasitic disease of humans and various herbivorous domestic animals (intermediate hosts) transmitted by the contact with wild and domestic carnivores (definitive hosts), mainly foxes and dogs. Recently, a vaccine was developed showing high levels of protection against one parasite haplotype (G1) of Echinococcus granulosus, and its potential efficacy against distinct parasite variants or species is still unclear. Interestingly, the EG95 vaccine antigen is a secreted glycosylphosphatydilinositol (GPI)-anchored protein containing a fibronectin type III domain, which is ubiquitous in modular proteins involved in cell adhesion. EG95 is highly expressed in oncospheres, the parasite life cycle stage which actively invades the intermediate hosts. After amplifying and sequencing the complete CDS of 57 Echinococcus isolates belonging to 7 distinct species, we uncovered a large amount of genetic variability, which may influence protein folding. Two positively selected sites are outside the vaccine epitopes, but are predicted to alter protein conformation. Moreover, phylogenetic analyses indicate that EG95 isoform evolution is convergent with regard to the number of beta-sheets and alpha-helices. We conclude that having a variety of EG95 isoforms is adaptive for Echinococcus parasites, in terms of their ability to invade different hosts, and we propose that a mixture of isoforms could possibly maximize vaccine efficacy. |
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Haag, Karen LuisaGottstein, BrunoAyala, Francisco J.2010-05-06T04:16:41Z20091932-6203http://hdl.handle.net/10183/21615000692673Echinococcosis is a worldwide zoonotic parasitic disease of humans and various herbivorous domestic animals (intermediate hosts) transmitted by the contact with wild and domestic carnivores (definitive hosts), mainly foxes and dogs. Recently, a vaccine was developed showing high levels of protection against one parasite haplotype (G1) of Echinococcus granulosus, and its potential efficacy against distinct parasite variants or species is still unclear. Interestingly, the EG95 vaccine antigen is a secreted glycosylphosphatydilinositol (GPI)-anchored protein containing a fibronectin type III domain, which is ubiquitous in modular proteins involved in cell adhesion. EG95 is highly expressed in oncospheres, the parasite life cycle stage which actively invades the intermediate hosts. After amplifying and sequencing the complete CDS of 57 Echinococcus isolates belonging to 7 distinct species, we uncovered a large amount of genetic variability, which may influence protein folding. Two positively selected sites are outside the vaccine epitopes, but are predicted to alter protein conformation. Moreover, phylogenetic analyses indicate that EG95 isoform evolution is convergent with regard to the number of beta-sheets and alpha-helices. We conclude that having a variety of EG95 isoforms is adaptive for Echinococcus parasites, in terms of their ability to invade different hosts, and we propose that a mixture of isoforms could possibly maximize vaccine efficacy.application/zipapplication/zipapplication/pdfengPlos One. San Francisco. Vol. 4, no. 4 (apr. 2009), e5362, 8 p.Echinococcus sppEchinococcus granulosusBiologia molecularThe EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacyEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000692673.pdf000692673.pdfTexto completo (inglês)application/pdf1110925http://www.lume.ufrgs.br/bitstream/10183/21615/1/000692673.pdff8f4867d71a303b70aa9995173549ed4MD51000692673.zip000692673.zipMaterial suplementarapplication/zip90177http://www.lume.ufrgs.br/bitstream/10183/21615/2/000692673.zipc1355c73d201ca9a52eb4db3e46435beMD52000692673-02.zip000692673-02.zipTrabalho completo zipadoapplication/zip1192521http://www.lume.ufrgs.br/bitstream/10183/21615/3/000692673-02.zip791444c24d6429f1036d51673bf5b86bMD53TEXT000692673.pdf.txt000692673.pdf.txtExtracted Texttext/plain40777http://www.lume.ufrgs.br/bitstream/10183/21615/4/000692673.pdf.txt2ba829661835cc031a62ccf309da961cMD54THUMBNAIL000692673.pdf.jpg000692673.pdf.jpgGenerated Thumbnailimage/jpeg2076http://www.lume.ufrgs.br/bitstream/10183/21615/5/000692673.pdf.jpg988553a7b2b0e069e4614753dc410344MD5510183/216152023-09-23 03:36:50.787163oai:www.lume.ufrgs.br:10183/21615Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-23T06:36:50Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy |
title |
The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy |
spellingShingle |
The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy Haag, Karen Luisa Echinococcus spp Echinococcus granulosus Biologia molecular |
title_short |
The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy |
title_full |
The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy |
title_fullStr |
The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy |
title_full_unstemmed |
The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy |
title_sort |
The EG95 antigen of Echinococcus spp. contains positively selected amino acids, which may influence host specificity and vaccine efficacy |
author |
Haag, Karen Luisa |
author_facet |
Haag, Karen Luisa Gottstein, Bruno Ayala, Francisco J. |
author_role |
author |
author2 |
Gottstein, Bruno Ayala, Francisco J. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Haag, Karen Luisa Gottstein, Bruno Ayala, Francisco J. |
dc.subject.por.fl_str_mv |
Echinococcus spp Echinococcus granulosus Biologia molecular |
topic |
Echinococcus spp Echinococcus granulosus Biologia molecular |
description |
Echinococcosis is a worldwide zoonotic parasitic disease of humans and various herbivorous domestic animals (intermediate hosts) transmitted by the contact with wild and domestic carnivores (definitive hosts), mainly foxes and dogs. Recently, a vaccine was developed showing high levels of protection against one parasite haplotype (G1) of Echinococcus granulosus, and its potential efficacy against distinct parasite variants or species is still unclear. Interestingly, the EG95 vaccine antigen is a secreted glycosylphosphatydilinositol (GPI)-anchored protein containing a fibronectin type III domain, which is ubiquitous in modular proteins involved in cell adhesion. EG95 is highly expressed in oncospheres, the parasite life cycle stage which actively invades the intermediate hosts. After amplifying and sequencing the complete CDS of 57 Echinococcus isolates belonging to 7 distinct species, we uncovered a large amount of genetic variability, which may influence protein folding. Two positively selected sites are outside the vaccine epitopes, but are predicted to alter protein conformation. Moreover, phylogenetic analyses indicate that EG95 isoform evolution is convergent with regard to the number of beta-sheets and alpha-helices. We conclude that having a variety of EG95 isoforms is adaptive for Echinococcus parasites, in terms of their ability to invade different hosts, and we propose that a mixture of isoforms could possibly maximize vaccine efficacy. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2010-05-06T04:16:41Z |
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Estrangeiro info:eu-repo/semantics/article |
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http://hdl.handle.net/10183/21615 |
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1932-6203 |
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000692673 |
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Plos One. San Francisco. Vol. 4, no. 4 (apr. 2009), e5362, 8 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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