Descripción cariotípica de Spheniscus magellanicus (Spheniscidae)

Detalhes bibliográficos
Autor(a) principal: Ledesma, Mario A.
Data de Publicação: 2003
Outros Autores: Freitas, Thales Renato Ochotorena de, Silva, Juliana da, Silva, Fernanda Rabaioli da, Gunski, Ricardo José
Tipo de documento: Artigo
Idioma: spa
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/27109
Resumo: The karyotype and the C-band pattern of Spheniscus magellanicus are described here for the first time. Peripheral blood samples from three females and four males were taken in Imbé (Rio Grande do Sul, Brazil). Long-time cultures of leukocytes were made in order to obtain mitotic metaphases. The diploid number was determined from the analysis of 30 metaphases. The karyotype of Spheniscus magellanicus presents 68 chromosomes. Pairs 1, 2, 4, 5 and 7 are submetacentric, pairs 6 and 10 are metacentric, pairs 3, 8 and 9 are telocentric, and the remaining ones are small telocentric chromosomes. The sex chromosomes Z and W are submetacentric. The study carried out with C bands demonstrated that the W chromosome is totally heterocromatic, while the Z chromosome does not present positive marks. Centromeric marks can be observed in the macrochromosomes and in some microchromosomes. The only observed difference found among this species and the previously analyzed Spheniscus demersus and Spheniscus humboldti, resides in the diploid number 72 and 78 of those species, respectively. These differences are observed exclusively at the level of microchromosomes, indicating a high homogeneity in the diploid number and chromosome morphology of this group.
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spelling Ledesma, Mario A.Freitas, Thales Renato Ochotorena deSilva, Juliana daSilva, Fernanda Rabaioli daGunski, Ricardo José2010-12-25T04:21:17Z20030073-3407http://hdl.handle.net/10183/27109000642191The karyotype and the C-band pattern of Spheniscus magellanicus are described here for the first time. Peripheral blood samples from three females and four males were taken in Imbé (Rio Grande do Sul, Brazil). Long-time cultures of leukocytes were made in order to obtain mitotic metaphases. The diploid number was determined from the analysis of 30 metaphases. The karyotype of Spheniscus magellanicus presents 68 chromosomes. Pairs 1, 2, 4, 5 and 7 are submetacentric, pairs 6 and 10 are metacentric, pairs 3, 8 and 9 are telocentric, and the remaining ones are small telocentric chromosomes. The sex chromosomes Z and W are submetacentric. The study carried out with C bands demonstrated that the W chromosome is totally heterocromatic, while the Z chromosome does not present positive marks. Centromeric marks can be observed in the macrochromosomes and in some microchromosomes. The only observed difference found among this species and the previously analyzed Spheniscus demersus and Spheniscus humboldti, resides in the diploid number 72 and 78 of those species, respectively. These differences are observed exclusively at the level of microchromosomes, indicating a high homogeneity in the diploid number and chromosome morphology of this group.En este trabajo se describe por primera vez el cariotipo y el patrón de bandas C de Spheniscus magellanicus. Se tomaron muestras de sangre periférica de tres hembras y cuatro machos en Imbé (Rio Grande do Sul, Brasil). Para la obtención de metafases mitóticas se utilizó la técnica de cultivos de linfocitos de larga duración. El número diploide fue determinado a partir del análisis de 30 metafases. El cariotipo de Spheniscus magellanicus posee 2n = 68 cromosomas. Los pares 1º, 2º, 4º, 5º y 7º son submetacéntricos, los pares 6º y 10º metacéntricos, los pares 3º, 8º y 9º telocéntricos, y los restantes son cromosomas telocéntricos pequeños. Los cromosomas sexuales Z y W son submetacéntricos. Los estudios realizados a través de bandeo C demostraron que el cromosoma W es totalmente heterocromático, mientras que el cromosoma Z no presenta marcaci ón positiva. En los macrocromosomas y en algunos microcromosomas pueden observarse marcaciones centroméricas. La única diferencia encontrada entre esta especie y las previamente analizadas Spheniscus demersus y Spheniscus humboldti, radica en el número diploide 72 y 78 de éstas últimas, respectivamente. Estas diferencias están dadas exclusivamente al nivel de los microcromosomas, lo que indica la elevada homogeneidad que existe en el número cromosómico y en la morfología cromosómica de este grupo de aves.application/pdfspaHornero. Buenos Aires. Vol. 18, n. 1 (Jan./ Aug. 2003), p. 37-40CariótipoCromossomosSpheniscus magellanicusC bandsKaryotypeMacrochromosomesMicrochromosomesSpheniscusDescripción cariotípica de Spheniscus magellanicus (Spheniscidae)Karyotypical description of spheniscus magellaniscus magellanicus (spheniscidae) Estrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000642191.pdf.txt000642191.pdf.txtExtracted Texttext/plain13347http://www.lume.ufrgs.br/bitstream/10183/27109/2/000642191.pdf.txt5d37e81418b2f2c4cef95ca576579bf9MD52ORIGINAL000642191.pdf000642191.pdfTexto completo (espanhol)application/pdf306503http://www.lume.ufrgs.br/bitstream/10183/27109/1/000642191.pdf498dd7f4e8d090c996d41a94bb522431MD51THUMBNAIL000642191.pdf.jpg000642191.pdf.jpgGenerated Thumbnailimage/jpeg1798http://www.lume.ufrgs.br/bitstream/10183/27109/3/000642191.pdf.jpge17a479b8165c850d23b0a70f1262211MD5310183/271092021-06-13 04:34:56.549993oai:www.lume.ufrgs.br:10183/27109Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-06-13T07:34:56Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Descripción cariotípica de Spheniscus magellanicus (Spheniscidae)
dc.title.alternative.en.fl_str_mv Karyotypical description of spheniscus magellaniscus magellanicus (spheniscidae)
title Descripción cariotípica de Spheniscus magellanicus (Spheniscidae)
spellingShingle Descripción cariotípica de Spheniscus magellanicus (Spheniscidae)
Ledesma, Mario A.
Cariótipo
Cromossomos
Spheniscus magellanicus
C bands
Karyotype
Macrochromosomes
Microchromosomes
Spheniscus
title_short Descripción cariotípica de Spheniscus magellanicus (Spheniscidae)
title_full Descripción cariotípica de Spheniscus magellanicus (Spheniscidae)
title_fullStr Descripción cariotípica de Spheniscus magellanicus (Spheniscidae)
title_full_unstemmed Descripción cariotípica de Spheniscus magellanicus (Spheniscidae)
title_sort Descripción cariotípica de Spheniscus magellanicus (Spheniscidae)
author Ledesma, Mario A.
author_facet Ledesma, Mario A.
Freitas, Thales Renato Ochotorena de
Silva, Juliana da
Silva, Fernanda Rabaioli da
Gunski, Ricardo José
author_role author
author2 Freitas, Thales Renato Ochotorena de
Silva, Juliana da
Silva, Fernanda Rabaioli da
Gunski, Ricardo José
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ledesma, Mario A.
Freitas, Thales Renato Ochotorena de
Silva, Juliana da
Silva, Fernanda Rabaioli da
Gunski, Ricardo José
dc.subject.por.fl_str_mv Cariótipo
Cromossomos
Spheniscus magellanicus
topic Cariótipo
Cromossomos
Spheniscus magellanicus
C bands
Karyotype
Macrochromosomes
Microchromosomes
Spheniscus
dc.subject.eng.fl_str_mv C bands
Karyotype
Macrochromosomes
Microchromosomes
Spheniscus
description The karyotype and the C-band pattern of Spheniscus magellanicus are described here for the first time. Peripheral blood samples from three females and four males were taken in Imbé (Rio Grande do Sul, Brazil). Long-time cultures of leukocytes were made in order to obtain mitotic metaphases. The diploid number was determined from the analysis of 30 metaphases. The karyotype of Spheniscus magellanicus presents 68 chromosomes. Pairs 1, 2, 4, 5 and 7 are submetacentric, pairs 6 and 10 are metacentric, pairs 3, 8 and 9 are telocentric, and the remaining ones are small telocentric chromosomes. The sex chromosomes Z and W are submetacentric. The study carried out with C bands demonstrated that the W chromosome is totally heterocromatic, while the Z chromosome does not present positive marks. Centromeric marks can be observed in the macrochromosomes and in some microchromosomes. The only observed difference found among this species and the previously analyzed Spheniscus demersus and Spheniscus humboldti, resides in the diploid number 72 and 78 of those species, respectively. These differences are observed exclusively at the level of microchromosomes, indicating a high homogeneity in the diploid number and chromosome morphology of this group.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2010-12-25T04:21:17Z
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dc.identifier.nrb.pt_BR.fl_str_mv 000642191
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url http://hdl.handle.net/10183/27109
dc.language.iso.fl_str_mv spa
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dc.relation.ispartof.pt_BR.fl_str_mv Hornero. Buenos Aires. Vol. 18, n. 1 (Jan./ Aug. 2003), p. 37-40
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