Zika virus envelope domain III recombinant protein delivered with saponin-based nanoadjuvant from Quillaja brasiliensis enhances anti-zika immune responses, including neutralizing antibodies and splenocyte proliferation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/220533 |
Resumo: | Nanoadjuvants that combine immunostimulatory properties and delivery systems reportedly bestow major improvements on the efficacy of recombinant, protein-based vaccines. Among these, self-assembled micellar formulations named ISCOMs (immune stimulating complexes) show a great ability to trigger powerful immunological responses against infectious pathogens. Here, a nanoadjuvant preparation, based on saponins from Quillaja brasiliensis, was evaluated together with an experimental Zika virus (ZIKV) vaccine (IQB80-zEDIII) and compared to an equivalent vaccine with alum as the standard adjuvant. The preparations were administered to mice in two doses (on days zero and 14) and immune responses were evaluated on day 28 post-priming. Serum levels of anti-Zika virus IgG, IgG1, IgG2b, IgG2c, IgG3 were significantly increased by the nanoadjuvant vaccine, compared to the mice that received the alum-adjuvanted vaccine or the unadjuvanted vaccine. In addition, a robust production of neutralizing antibodies and in vitro splenocyte proliferative responses were observed in mice immunized with IQB80-zEDIII nanoformulated vaccine. Therefore, the IQB80-zEDIII recombinant preparation seems to be a suitable candidate vaccine for ZIKV. Overall, this study identified saponin-based delivery systems as an adequate adjuvant for recombinant ZIKV vaccines and has important implications for recombinant protein-based vaccine formulations against other flaviviruses and possibly enveloped viruses. |
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Cibulski, Samuel PauloVarela, Ana Paula MuterleTeixeira, Thais FumacoCancela, MartínSesterheim, PatríciaSouza, Diogo Onofre Gomes deRoehe, Paulo MichelSilveira, Fernando2021-05-05T04:27:22Z20211664-3224http://hdl.handle.net/10183/220533001125019Nanoadjuvants that combine immunostimulatory properties and delivery systems reportedly bestow major improvements on the efficacy of recombinant, protein-based vaccines. Among these, self-assembled micellar formulations named ISCOMs (immune stimulating complexes) show a great ability to trigger powerful immunological responses against infectious pathogens. Here, a nanoadjuvant preparation, based on saponins from Quillaja brasiliensis, was evaluated together with an experimental Zika virus (ZIKV) vaccine (IQB80-zEDIII) and compared to an equivalent vaccine with alum as the standard adjuvant. The preparations were administered to mice in two doses (on days zero and 14) and immune responses were evaluated on day 28 post-priming. Serum levels of anti-Zika virus IgG, IgG1, IgG2b, IgG2c, IgG3 were significantly increased by the nanoadjuvant vaccine, compared to the mice that received the alum-adjuvanted vaccine or the unadjuvanted vaccine. In addition, a robust production of neutralizing antibodies and in vitro splenocyte proliferative responses were observed in mice immunized with IQB80-zEDIII nanoformulated vaccine. Therefore, the IQB80-zEDIII recombinant preparation seems to be a suitable candidate vaccine for ZIKV. Overall, this study identified saponin-based delivery systems as an adequate adjuvant for recombinant ZIKV vaccines and has important implications for recombinant protein-based vaccine formulations against other flaviviruses and possibly enveloped viruses.application/pdfengFrontiers in immunology. Lausanne. Vol. 12 (Mar. 2021), 632714, 13 p.Saponinas de quilaiaZika virusImunoterapia ativaAutoimunidadeAdjuvantes imunologicosISCOMZika virusFlavivirusEmerging diseasesQuillajaceaeZika virus envelope domain III recombinant protein delivered with saponin-based nanoadjuvant from Quillaja brasiliensis enhances anti-zika immune responses, including neutralizing antibodies and splenocyte proliferationEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001125019.pdf.txt001125019.pdf.txtExtracted Texttext/plain62273http://www.lume.ufrgs.br/bitstream/10183/220533/2/001125019.pdf.txtd5238b675bb3cd280f74b7d56dbd20afMD52ORIGINAL001125019.pdfTexto completo (inglês)application/pdf3134886http://www.lume.ufrgs.br/bitstream/10183/220533/1/001125019.pdf4f44521ff563bd0b83a5604c64050172MD5110183/2205332021-05-07 04:37:28.843464oai:www.lume.ufrgs.br:10183/220533Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-05-07T07:37:28Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Zika virus envelope domain III recombinant protein delivered with saponin-based nanoadjuvant from Quillaja brasiliensis enhances anti-zika immune responses, including neutralizing antibodies and splenocyte proliferation |
title |
Zika virus envelope domain III recombinant protein delivered with saponin-based nanoadjuvant from Quillaja brasiliensis enhances anti-zika immune responses, including neutralizing antibodies and splenocyte proliferation |
spellingShingle |
Zika virus envelope domain III recombinant protein delivered with saponin-based nanoadjuvant from Quillaja brasiliensis enhances anti-zika immune responses, including neutralizing antibodies and splenocyte proliferation Cibulski, Samuel Paulo Saponinas de quilaia Zika virus Imunoterapia ativa Autoimunidade Adjuvantes imunologicos ISCOM Zika virus Flavivirus Emerging diseases Quillajaceae |
title_short |
Zika virus envelope domain III recombinant protein delivered with saponin-based nanoadjuvant from Quillaja brasiliensis enhances anti-zika immune responses, including neutralizing antibodies and splenocyte proliferation |
title_full |
Zika virus envelope domain III recombinant protein delivered with saponin-based nanoadjuvant from Quillaja brasiliensis enhances anti-zika immune responses, including neutralizing antibodies and splenocyte proliferation |
title_fullStr |
Zika virus envelope domain III recombinant protein delivered with saponin-based nanoadjuvant from Quillaja brasiliensis enhances anti-zika immune responses, including neutralizing antibodies and splenocyte proliferation |
title_full_unstemmed |
Zika virus envelope domain III recombinant protein delivered with saponin-based nanoadjuvant from Quillaja brasiliensis enhances anti-zika immune responses, including neutralizing antibodies and splenocyte proliferation |
title_sort |
Zika virus envelope domain III recombinant protein delivered with saponin-based nanoadjuvant from Quillaja brasiliensis enhances anti-zika immune responses, including neutralizing antibodies and splenocyte proliferation |
author |
Cibulski, Samuel Paulo |
author_facet |
Cibulski, Samuel Paulo Varela, Ana Paula Muterle Teixeira, Thais Fumaco Cancela, Martín Sesterheim, Patrícia Souza, Diogo Onofre Gomes de Roehe, Paulo Michel Silveira, Fernando |
author_role |
author |
author2 |
Varela, Ana Paula Muterle Teixeira, Thais Fumaco Cancela, Martín Sesterheim, Patrícia Souza, Diogo Onofre Gomes de Roehe, Paulo Michel Silveira, Fernando |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Cibulski, Samuel Paulo Varela, Ana Paula Muterle Teixeira, Thais Fumaco Cancela, Martín Sesterheim, Patrícia Souza, Diogo Onofre Gomes de Roehe, Paulo Michel Silveira, Fernando |
dc.subject.por.fl_str_mv |
Saponinas de quilaia Zika virus Imunoterapia ativa Autoimunidade Adjuvantes imunologicos |
topic |
Saponinas de quilaia Zika virus Imunoterapia ativa Autoimunidade Adjuvantes imunologicos ISCOM Zika virus Flavivirus Emerging diseases Quillajaceae |
dc.subject.eng.fl_str_mv |
ISCOM Zika virus Flavivirus Emerging diseases Quillajaceae |
description |
Nanoadjuvants that combine immunostimulatory properties and delivery systems reportedly bestow major improvements on the efficacy of recombinant, protein-based vaccines. Among these, self-assembled micellar formulations named ISCOMs (immune stimulating complexes) show a great ability to trigger powerful immunological responses against infectious pathogens. Here, a nanoadjuvant preparation, based on saponins from Quillaja brasiliensis, was evaluated together with an experimental Zika virus (ZIKV) vaccine (IQB80-zEDIII) and compared to an equivalent vaccine with alum as the standard adjuvant. The preparations were administered to mice in two doses (on days zero and 14) and immune responses were evaluated on day 28 post-priming. Serum levels of anti-Zika virus IgG, IgG1, IgG2b, IgG2c, IgG3 were significantly increased by the nanoadjuvant vaccine, compared to the mice that received the alum-adjuvanted vaccine or the unadjuvanted vaccine. In addition, a robust production of neutralizing antibodies and in vitro splenocyte proliferative responses were observed in mice immunized with IQB80-zEDIII nanoformulated vaccine. Therefore, the IQB80-zEDIII recombinant preparation seems to be a suitable candidate vaccine for ZIKV. Overall, this study identified saponin-based delivery systems as an adequate adjuvant for recombinant ZIKV vaccines and has important implications for recombinant protein-based vaccine formulations against other flaviviruses and possibly enveloped viruses. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-05-05T04:27:22Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/220533 |
dc.identifier.issn.pt_BR.fl_str_mv |
1664-3224 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001125019 |
identifier_str_mv |
1664-3224 001125019 |
url |
http://hdl.handle.net/10183/220533 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Frontiers in immunology. Lausanne. Vol. 12 (Mar. 2021), 632714, 13 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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