Detection and genetic characterization of Mamastrovirus 5 from brazilian dogs
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/184051 |
Resumo: | Mamastrovirus 5 (MAstV5), belonging to the Astroviridae (AstV) family, previously knownas canine astrovirus or astrovirus-like particles, has been reported in several countries tobe associated with viral enteric disease in dogs since the 1980s. Astroviruses have beendetected in fecal samples from a wide variety of mammals and birds that are associ-ated with gastroenteritis and extra enteric manifestations. In the present study, RT-PCRwas used to investigate the presence of MAstV5 in 269 dog fecal samples. MAstV5 wasdetected in 26% (71/269) of the samples. Interestingly, all MAstV5-positive samples derivedfrom dogs displaying clinical signs suggestive of gastroenteritis, other enteric viruses weresimultaneously detected (canine parvovirus, canine distemper virus, canine coronavirus,canine adenovirus and canine rotavirus). Based on genomic sequence analysis of MAstV5a novel classification of the species into four genotypes, MAstV5a-MAstV5d, is proposed.Phylogenetic analyses based on the ORF2 amino acid sequences, samples described hereingrouped into the putative genotype ‘a’ closed related with Chinese samples. Other studiesare required to attempt the clinical and antigenic implications of these astrovirus genotypesin dogs. |
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Alves, Christian Diniz Beduschi TravassosBudaszewski, Renata da FontouraTorikachvili, MarcelaStreck, André FelipeWeber, Matheus NunesCibulski, Samuel PauloRavazzolo, Ana PaulaLunge, Vagner RicardoCanal, Cláudio Wageck2018-10-27T03:12:28Z20181517-8382http://hdl.handle.net/10183/184051001077989Mamastrovirus 5 (MAstV5), belonging to the Astroviridae (AstV) family, previously knownas canine astrovirus or astrovirus-like particles, has been reported in several countries tobe associated with viral enteric disease in dogs since the 1980s. Astroviruses have beendetected in fecal samples from a wide variety of mammals and birds that are associ-ated with gastroenteritis and extra enteric manifestations. In the present study, RT-PCRwas used to investigate the presence of MAstV5 in 269 dog fecal samples. MAstV5 wasdetected in 26% (71/269) of the samples. Interestingly, all MAstV5-positive samples derivedfrom dogs displaying clinical signs suggestive of gastroenteritis, other enteric viruses weresimultaneously detected (canine parvovirus, canine distemper virus, canine coronavirus,canine adenovirus and canine rotavirus). Based on genomic sequence analysis of MAstV5a novel classification of the species into four genotypes, MAstV5a-MAstV5d, is proposed.Phylogenetic analyses based on the ORF2 amino acid sequences, samples described hereingrouped into the putative genotype ‘a’ closed related with Chinese samples. Other studiesare required to attempt the clinical and antigenic implications of these astrovirus genotypesin dogs.application/pdfengBrazilian Journal of Microbiology. São Paulo. Vol. 49, no. 3 (July 2018), p. 575-583Virologia veterinariaMamastrovirusGastroenteriteAnálise de sequência de DNAFilogeniaCãesMamastrovirus 5Canine astrovirusDogGastroenteritisMAstV5Detection and genetic characterization of Mamastrovirus 5 from brazilian dogsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001077989.pdfTexto completo (inglês)application/pdf959140http://www.lume.ufrgs.br/bitstream/10183/184051/1/001077989.pdf62a2839c2d297b684d80a8251d3c0d13MD51TEXT001077989.pdf.txt001077989.pdf.txtExtracted Texttext/plain36585http://www.lume.ufrgs.br/bitstream/10183/184051/2/001077989.pdf.txt3faee6324d39f7a7324b05d8219a2311MD52THUMBNAIL001077989.pdf.jpg001077989.pdf.jpgGenerated Thumbnailimage/jpeg1948http://www.lume.ufrgs.br/bitstream/10183/184051/3/001077989.pdf.jpge8134e0c979eb1f280af3f03ea7155e5MD5310183/1840512018-11-09 02:45:54.703196oai:www.lume.ufrgs.br:10183/184051Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-11-09T04:45:54Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Detection and genetic characterization of Mamastrovirus 5 from brazilian dogs |
title |
Detection and genetic characterization of Mamastrovirus 5 from brazilian dogs |
spellingShingle |
Detection and genetic characterization of Mamastrovirus 5 from brazilian dogs Alves, Christian Diniz Beduschi Travassos Virologia veterinaria Mamastrovirus Gastroenterite Análise de sequência de DNA Filogenia Cães Mamastrovirus 5 Canine astrovirus Dog Gastroenteritis MAstV5 |
title_short |
Detection and genetic characterization of Mamastrovirus 5 from brazilian dogs |
title_full |
Detection and genetic characterization of Mamastrovirus 5 from brazilian dogs |
title_fullStr |
Detection and genetic characterization of Mamastrovirus 5 from brazilian dogs |
title_full_unstemmed |
Detection and genetic characterization of Mamastrovirus 5 from brazilian dogs |
title_sort |
Detection and genetic characterization of Mamastrovirus 5 from brazilian dogs |
author |
Alves, Christian Diniz Beduschi Travassos |
author_facet |
Alves, Christian Diniz Beduschi Travassos Budaszewski, Renata da Fontoura Torikachvili, Marcela Streck, André Felipe Weber, Matheus Nunes Cibulski, Samuel Paulo Ravazzolo, Ana Paula Lunge, Vagner Ricardo Canal, Cláudio Wageck |
author_role |
author |
author2 |
Budaszewski, Renata da Fontoura Torikachvili, Marcela Streck, André Felipe Weber, Matheus Nunes Cibulski, Samuel Paulo Ravazzolo, Ana Paula Lunge, Vagner Ricardo Canal, Cláudio Wageck |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Alves, Christian Diniz Beduschi Travassos Budaszewski, Renata da Fontoura Torikachvili, Marcela Streck, André Felipe Weber, Matheus Nunes Cibulski, Samuel Paulo Ravazzolo, Ana Paula Lunge, Vagner Ricardo Canal, Cláudio Wageck |
dc.subject.por.fl_str_mv |
Virologia veterinaria Mamastrovirus Gastroenterite Análise de sequência de DNA Filogenia Cães |
topic |
Virologia veterinaria Mamastrovirus Gastroenterite Análise de sequência de DNA Filogenia Cães Mamastrovirus 5 Canine astrovirus Dog Gastroenteritis MAstV5 |
dc.subject.eng.fl_str_mv |
Mamastrovirus 5 Canine astrovirus Dog Gastroenteritis MAstV5 |
description |
Mamastrovirus 5 (MAstV5), belonging to the Astroviridae (AstV) family, previously knownas canine astrovirus or astrovirus-like particles, has been reported in several countries tobe associated with viral enteric disease in dogs since the 1980s. Astroviruses have beendetected in fecal samples from a wide variety of mammals and birds that are associ-ated with gastroenteritis and extra enteric manifestations. In the present study, RT-PCRwas used to investigate the presence of MAstV5 in 269 dog fecal samples. MAstV5 wasdetected in 26% (71/269) of the samples. Interestingly, all MAstV5-positive samples derivedfrom dogs displaying clinical signs suggestive of gastroenteritis, other enteric viruses weresimultaneously detected (canine parvovirus, canine distemper virus, canine coronavirus,canine adenovirus and canine rotavirus). Based on genomic sequence analysis of MAstV5a novel classification of the species into four genotypes, MAstV5a-MAstV5d, is proposed.Phylogenetic analyses based on the ORF2 amino acid sequences, samples described hereingrouped into the putative genotype ‘a’ closed related with Chinese samples. Other studiesare required to attempt the clinical and antigenic implications of these astrovirus genotypesin dogs. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-10-27T03:12:28Z |
dc.date.issued.fl_str_mv |
2018 |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/184051 |
dc.identifier.issn.pt_BR.fl_str_mv |
1517-8382 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001077989 |
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1517-8382 001077989 |
url |
http://hdl.handle.net/10183/184051 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian Journal of Microbiology. São Paulo. Vol. 49, no. 3 (July 2018), p. 575-583 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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