Isolation and characterization of microsatellites from the tapeworm Echinococcus granulosus

Detalhes bibliográficos
Autor(a) principal: Bartholomei-Santos, Marlise Ladvocat
Data de Publicação: 2003
Outros Autores: Heinzelmann, Larissa Schemes, Oliveira, R. P., Chemale, Gustavo, Gutierrez, Ariana M., Kamenetzky, Laura, Haag, Karen Luisa, Zaha, Arnaldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/27455
Resumo: The Echinococcus granulosus genome was searched for microsatellites using 8 different repeated oligonucleotides as probes (GT15, CT15, AT15, CG15, CAT10, CAA10, CGG10 and CATA10). Southern blot experiments revealed that DNA regions containing GT, CAA, CATA and CT repeats are the most frequent in the E. granulosus genome. AT and CG probes showed no hybridization signal. Two loci containing CA/GT (Egmsca1 and Egmsca2) and 1 locus containing GA/CT (Egmsga1) repeats were cloned and sequenced. The locus Egmsca1 was analysed in 73 isolates from Brazil and Argentina whose strains were previously characterized. Brazilian isolates from cattle strain and Argentinean isolates from camel strain were monomorphic and shared the allele (CA)7. Argentinean isolates of sheep and Tasmanian sheep strains shared 2 alleles [(CA)8 and (CA)10] with Brazilian isolates of sheep strain. The allele (CA)11 was found only in Brazilian isolates of sheep strain at a low frequency. The Brazilian and the Argentinean sheep strain populations were tested for the Hardy- Weinberg equilibrium, and only the former was in agreement with the expectations. No polymorphism was found among individual protoscoleces from a single hydatid cyst, validating the utilization of pooled protoscoleces from 1 cyst, grouped as an isolate, in population studies. This work describes for the first time the isolation and characterization of microsatellites from E. granulosus.
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spelling Bartholomei-Santos, Marlise LadvocatHeinzelmann, Larissa SchemesOliveira, R. P.Chemale, GustavoGutierrez, Ariana M.Kamenetzky, LauraHaag, Karen LuisaZaha, Arnaldo2011-01-21T05:59:01Z20030031-1820http://hdl.handle.net/10183/27455000390669The Echinococcus granulosus genome was searched for microsatellites using 8 different repeated oligonucleotides as probes (GT15, CT15, AT15, CG15, CAT10, CAA10, CGG10 and CATA10). Southern blot experiments revealed that DNA regions containing GT, CAA, CATA and CT repeats are the most frequent in the E. granulosus genome. AT and CG probes showed no hybridization signal. Two loci containing CA/GT (Egmsca1 and Egmsca2) and 1 locus containing GA/CT (Egmsga1) repeats were cloned and sequenced. The locus Egmsca1 was analysed in 73 isolates from Brazil and Argentina whose strains were previously characterized. Brazilian isolates from cattle strain and Argentinean isolates from camel strain were monomorphic and shared the allele (CA)7. Argentinean isolates of sheep and Tasmanian sheep strains shared 2 alleles [(CA)8 and (CA)10] with Brazilian isolates of sheep strain. The allele (CA)11 was found only in Brazilian isolates of sheep strain at a low frequency. The Brazilian and the Argentinean sheep strain populations were tested for the Hardy- Weinberg equilibrium, and only the former was in agreement with the expectations. No polymorphism was found among individual protoscoleces from a single hydatid cyst, validating the utilization of pooled protoscoleces from 1 cyst, grouped as an isolate, in population studies. This work describes for the first time the isolation and characterization of microsatellites from E. granulosus.application/pdfengParasitology. Cambridge, United Kingdom. Vol. 126 (2003), p. 599-605Echinococcus granulosusCestodaMicrossatéliteMarcador molecularEchinococcus granulosusCestodaMicrosatellite markersIsolation and characterization of microsatellites from the tapeworm Echinococcus granulosusEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000390669.pdf000390669.pdfTexto completo (inglês)application/pdf3079618http://www.lume.ufrgs.br/bitstream/10183/27455/1/000390669.pdf83637ec8f3772040bff9b7a15480fbdeMD51TEXT000390669.pdf.txt000390669.pdf.txtExtracted Texttext/plain26275http://www.lume.ufrgs.br/bitstream/10183/27455/2/000390669.pdf.txt95ed4f5101e7e6dccc7079a0079c86c0MD52THUMBNAIL000390669.pdf.jpg000390669.pdf.jpgGenerated Thumbnailimage/jpeg1878http://www.lume.ufrgs.br/bitstream/10183/27455/3/000390669.pdf.jpg52e3e61aa9a105f5029162559c86774aMD5310183/274552021-06-13 04:34:19.529583oai:www.lume.ufrgs.br:10183/27455Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-06-13T07:34:19Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Isolation and characterization of microsatellites from the tapeworm Echinococcus granulosus
title Isolation and characterization of microsatellites from the tapeworm Echinococcus granulosus
spellingShingle Isolation and characterization of microsatellites from the tapeworm Echinococcus granulosus
Bartholomei-Santos, Marlise Ladvocat
Echinococcus granulosus
Cestoda
Microssatélite
Marcador molecular
Echinococcus granulosus
Cestoda
Microsatellite markers
title_short Isolation and characterization of microsatellites from the tapeworm Echinococcus granulosus
title_full Isolation and characterization of microsatellites from the tapeworm Echinococcus granulosus
title_fullStr Isolation and characterization of microsatellites from the tapeworm Echinococcus granulosus
title_full_unstemmed Isolation and characterization of microsatellites from the tapeworm Echinococcus granulosus
title_sort Isolation and characterization of microsatellites from the tapeworm Echinococcus granulosus
author Bartholomei-Santos, Marlise Ladvocat
author_facet Bartholomei-Santos, Marlise Ladvocat
Heinzelmann, Larissa Schemes
Oliveira, R. P.
Chemale, Gustavo
Gutierrez, Ariana M.
Kamenetzky, Laura
Haag, Karen Luisa
Zaha, Arnaldo
author_role author
author2 Heinzelmann, Larissa Schemes
Oliveira, R. P.
Chemale, Gustavo
Gutierrez, Ariana M.
Kamenetzky, Laura
Haag, Karen Luisa
Zaha, Arnaldo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bartholomei-Santos, Marlise Ladvocat
Heinzelmann, Larissa Schemes
Oliveira, R. P.
Chemale, Gustavo
Gutierrez, Ariana M.
Kamenetzky, Laura
Haag, Karen Luisa
Zaha, Arnaldo
dc.subject.por.fl_str_mv Echinococcus granulosus
Cestoda
Microssatélite
Marcador molecular
topic Echinococcus granulosus
Cestoda
Microssatélite
Marcador molecular
Echinococcus granulosus
Cestoda
Microsatellite markers
dc.subject.eng.fl_str_mv Echinococcus granulosus
Cestoda
Microsatellite markers
description The Echinococcus granulosus genome was searched for microsatellites using 8 different repeated oligonucleotides as probes (GT15, CT15, AT15, CG15, CAT10, CAA10, CGG10 and CATA10). Southern blot experiments revealed that DNA regions containing GT, CAA, CATA and CT repeats are the most frequent in the E. granulosus genome. AT and CG probes showed no hybridization signal. Two loci containing CA/GT (Egmsca1 and Egmsca2) and 1 locus containing GA/CT (Egmsga1) repeats were cloned and sequenced. The locus Egmsca1 was analysed in 73 isolates from Brazil and Argentina whose strains were previously characterized. Brazilian isolates from cattle strain and Argentinean isolates from camel strain were monomorphic and shared the allele (CA)7. Argentinean isolates of sheep and Tasmanian sheep strains shared 2 alleles [(CA)8 and (CA)10] with Brazilian isolates of sheep strain. The allele (CA)11 was found only in Brazilian isolates of sheep strain at a low frequency. The Brazilian and the Argentinean sheep strain populations were tested for the Hardy- Weinberg equilibrium, and only the former was in agreement with the expectations. No polymorphism was found among individual protoscoleces from a single hydatid cyst, validating the utilization of pooled protoscoleces from 1 cyst, grouped as an isolate, in population studies. This work describes for the first time the isolation and characterization of microsatellites from E. granulosus.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2011-01-21T05:59:01Z
dc.type.driver.fl_str_mv Estrangeiro
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dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/27455
dc.identifier.issn.pt_BR.fl_str_mv 0031-1820
dc.identifier.nrb.pt_BR.fl_str_mv 000390669
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url http://hdl.handle.net/10183/27455
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Parasitology. Cambridge, United Kingdom. Vol. 126 (2003), p. 599-605
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
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reponame_str Repositório Institucional da UFRGS
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