Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/21159 |
Resumo: | We report here for the first time the structure and function of a promoter from a cestode. The ability of DNA fragments respectively encompassing the 935-bp and 524-bp regions upstream from the ATG codon from the EgactI and EgactII actin genes of Echinococcus granulosus to promote transcription was studied in the NIH3T3 mouse cell line. The results of transfection assays showed that both regions have strong promoter activity in these cells. The fragments were tested in both orientations and the 524-bp fragment of EgactII presented a bidirectional promoter activity. Deletion analysis of EgactI and EgactII promoters indicated the presence of regulatory regions containing putative silencer elements. These results indicate that both EgactI and EgactII promoters are functional and that the preliminary functional evaluation of E. granulosus and possibly of other cestode promoters can be performed in heterologous cell lines. |
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Gimba, Etel Rodrigues PereiraChemale, GustavoFarias, Sandra EstrazulasZaha, Arnaldo2010-04-24T04:15:32Z20000100-879Xhttp://hdl.handle.net/10183/21159000296773We report here for the first time the structure and function of a promoter from a cestode. The ability of DNA fragments respectively encompassing the 935-bp and 524-bp regions upstream from the ATG codon from the EgactI and EgactII actin genes of Echinococcus granulosus to promote transcription was studied in the NIH3T3 mouse cell line. The results of transfection assays showed that both regions have strong promoter activity in these cells. The fragments were tested in both orientations and the 524-bp fragment of EgactII presented a bidirectional promoter activity. Deletion analysis of EgactI and EgactII promoters indicated the presence of regulatory regions containing putative silencer elements. These results indicate that both EgactI and EgactII promoters are functional and that the preliminary functional evaluation of E. granulosus and possibly of other cestode promoters can be performed in heterologous cell lines.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 33, no. 11 (Nov. 2000), p. 1283-1292GenéticaEchinococcus granulosusActinPromotersCestodeGene expressionDeletionsCloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell lineinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000296773.pdf000296773.pdfTexto completo (inglês)application/pdf198344http://www.lume.ufrgs.br/bitstream/10183/21159/1/000296773.pdf58ad8606a2af85d21b209e0513b25bd8MD51TEXT000296773.pdf.txt000296773.pdf.txtExtracted Texttext/plain37885http://www.lume.ufrgs.br/bitstream/10183/21159/2/000296773.pdf.txt38faf41b4ca80a687ca5f7f47180383cMD52THUMBNAIL000296773.pdf.jpg000296773.pdf.jpgGenerated Thumbnailimage/jpeg1598http://www.lume.ufrgs.br/bitstream/10183/21159/3/000296773.pdf.jpgde80b2dbe48beccefbf4fab100199380MD5310183/211592018-10-09 08:58:47.87oai:www.lume.ufrgs.br:10183/21159Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-09T11:58:47Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line |
title |
Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line |
spellingShingle |
Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line Gimba, Etel Rodrigues Pereira Genética Echinococcus granulosus Actin Promoters Cestode Gene expression Deletions |
title_short |
Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line |
title_full |
Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line |
title_fullStr |
Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line |
title_full_unstemmed |
Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line |
title_sort |
Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line |
author |
Gimba, Etel Rodrigues Pereira |
author_facet |
Gimba, Etel Rodrigues Pereira Chemale, Gustavo Farias, Sandra Estrazulas Zaha, Arnaldo |
author_role |
author |
author2 |
Chemale, Gustavo Farias, Sandra Estrazulas Zaha, Arnaldo |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Gimba, Etel Rodrigues Pereira Chemale, Gustavo Farias, Sandra Estrazulas Zaha, Arnaldo |
dc.subject.por.fl_str_mv |
Genética |
topic |
Genética Echinococcus granulosus Actin Promoters Cestode Gene expression Deletions |
dc.subject.eng.fl_str_mv |
Echinococcus granulosus Actin Promoters Cestode Gene expression Deletions |
description |
We report here for the first time the structure and function of a promoter from a cestode. The ability of DNA fragments respectively encompassing the 935-bp and 524-bp regions upstream from the ATG codon from the EgactI and EgactII actin genes of Echinococcus granulosus to promote transcription was studied in the NIH3T3 mouse cell line. The results of transfection assays showed that both regions have strong promoter activity in these cells. The fragments were tested in both orientations and the 524-bp fragment of EgactII presented a bidirectional promoter activity. Deletion analysis of EgactI and EgactII promoters indicated the presence of regulatory regions containing putative silencer elements. These results indicate that both EgactI and EgactII promoters are functional and that the preliminary functional evaluation of E. granulosus and possibly of other cestode promoters can be performed in heterologous cell lines. |
publishDate |
2000 |
dc.date.issued.fl_str_mv |
2000 |
dc.date.accessioned.fl_str_mv |
2010-04-24T04:15:32Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10183/21159 |
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0100-879X |
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000296773 |
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http://hdl.handle.net/10183/21159 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 33, no. 11 (Nov. 2000), p. 1283-1292 |
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openAccess |
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