Optimizing fixation protocols to improve molecular analysis from FFPE tissues

Detalhes bibliográficos
Autor(a) principal: Maraschin, Bruna Jalfim
Data de Publicação: 2017
Outros Autores: Silva, Viviane Palmeira da, Rock, Leigha, Sun, Huichen, Visioli, Fernanda, Rados, Pantelis Varvaki, Rosin, Miriam P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/163919
Resumo: Most Departments of Pathology around the world have a considerable archive of formalinfixed paraffin-embedded (FFPE) tissue suitable for molecular assessment. This article points out the potential DNA damage that may occur if basic steps are not followed during processing and storage of these samples. Furthermore, it hopes to establish parameters to optimize quality and quantity of DNA extracted from FFPE tissues.
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spelling Maraschin, Bruna JalfimSilva, Viviane Palmeira daRock, LeighaSun, HuichenVisioli, FernandaRados, Pantelis VarvakiRosin, Miriam P.2017-07-12T02:30:39Z20170103-6440http://hdl.handle.net/10183/163919001024725Most Departments of Pathology around the world have a considerable archive of formalinfixed paraffin-embedded (FFPE) tissue suitable for molecular assessment. This article points out the potential DNA damage that may occur if basic steps are not followed during processing and storage of these samples. Furthermore, it hopes to establish parameters to optimize quality and quantity of DNA extracted from FFPE tissues.application/pdfengBrazilian dental journal. Ribeirão Preto. Vol. 28, no. 1 (Jan./Feb. 2017), p. 82-84Fixação de tecidosFormaldeídoFormaldehydeFormalinTissue fixationFixativesDNA extractionOptimizing fixation protocols to improve molecular analysis from FFPE tissuesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001024725.pdf001024725.pdfTexto completo (inglês)application/pdf370161http://www.lume.ufrgs.br/bitstream/10183/163919/1/001024725.pdf51bd31a0d157bab32c3bd644a20026cfMD51TEXT001024725.pdf.txt001024725.pdf.txtExtracted Texttext/plain13037http://www.lume.ufrgs.br/bitstream/10183/163919/2/001024725.pdf.txtd22b06bfccb85eb83d3bad7f94af9fe7MD52THUMBNAIL001024725.pdf.jpg001024725.pdf.jpgGenerated Thumbnailimage/jpeg1918http://www.lume.ufrgs.br/bitstream/10183/163919/3/001024725.pdf.jpgf2cb030e36c116a9427ac85f2c814defMD5310183/1639192018-10-10 07:50:18.041oai:www.lume.ufrgs.br:10183/163919Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-10T10:50:18Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Optimizing fixation protocols to improve molecular analysis from FFPE tissues
title Optimizing fixation protocols to improve molecular analysis from FFPE tissues
spellingShingle Optimizing fixation protocols to improve molecular analysis from FFPE tissues
Maraschin, Bruna Jalfim
Fixação de tecidos
Formaldeído
Formaldehyde
Formalin
Tissue fixation
Fixatives
DNA extraction
title_short Optimizing fixation protocols to improve molecular analysis from FFPE tissues
title_full Optimizing fixation protocols to improve molecular analysis from FFPE tissues
title_fullStr Optimizing fixation protocols to improve molecular analysis from FFPE tissues
title_full_unstemmed Optimizing fixation protocols to improve molecular analysis from FFPE tissues
title_sort Optimizing fixation protocols to improve molecular analysis from FFPE tissues
author Maraschin, Bruna Jalfim
author_facet Maraschin, Bruna Jalfim
Silva, Viviane Palmeira da
Rock, Leigha
Sun, Huichen
Visioli, Fernanda
Rados, Pantelis Varvaki
Rosin, Miriam P.
author_role author
author2 Silva, Viviane Palmeira da
Rock, Leigha
Sun, Huichen
Visioli, Fernanda
Rados, Pantelis Varvaki
Rosin, Miriam P.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Maraschin, Bruna Jalfim
Silva, Viviane Palmeira da
Rock, Leigha
Sun, Huichen
Visioli, Fernanda
Rados, Pantelis Varvaki
Rosin, Miriam P.
dc.subject.por.fl_str_mv Fixação de tecidos
Formaldeído
topic Fixação de tecidos
Formaldeído
Formaldehyde
Formalin
Tissue fixation
Fixatives
DNA extraction
dc.subject.eng.fl_str_mv Formaldehyde
Formalin
Tissue fixation
Fixatives
DNA extraction
description Most Departments of Pathology around the world have a considerable archive of formalinfixed paraffin-embedded (FFPE) tissue suitable for molecular assessment. This article points out the potential DNA damage that may occur if basic steps are not followed during processing and storage of these samples. Furthermore, it hopes to establish parameters to optimize quality and quantity of DNA extracted from FFPE tissues.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-07-12T02:30:39Z
dc.date.issued.fl_str_mv 2017
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/163919
dc.identifier.issn.pt_BR.fl_str_mv 0103-6440
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Brazilian dental journal. Ribeirão Preto. Vol. 28, no. 1 (Jan./Feb. 2017), p. 82-84
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