ALTERNATIVE FIXATION IN CYTOLOGICAL PROCESS

• Autor(a) principal: Carvalho, Marcio Santos
• Data de Publicação: 2020
• Outros Autores: de Sousa, Joana Albuquerque Bastos, de Macedo, Rayssa Ferreira Cavaleiro, de Sousa, Nayanna Matos, Mendes, Roberta Janaina Soares, Bezerra, Arthur Antunes Costa, Rodrigues, Vandilson Pinheiro, Pereira, Erika Martins
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Uningá (Online)
• Texto Completo: https://revista.uninga.br/uninga/article/view/3157
• topic: Citologia Esfoliativa; Fixadores; Formol; Citologia; Cytology; Fixatives; Formaldehyde; Cytology

The fixatives that are used for cytological routine are 96% ethanol and 10% formaldehyde. In the last decade, alternative fixatives have emerged, which show variations in their composition, in addition to containing lower concentrations of alcohol and the non-use of formaldehyde since the latter has carcinogenic properties. Among these, are of her alcoholic fixatives, such as RCL2 and some alternatives such as honey, brown sugar and hair styling spray, alt hough only few studies have proven the efficiency of these other fasteners. The objective of this study was to evaluate the effectiveness of an alternative fixative to formaldehyde and to present the conditions of acquisition in places and situations where formaldehyde is not available. Samples of the oral mucosa and dorsum of the tongue of the research participants were collected. These samples were fixed and divided into groups according to the following fixatives: RCL2, 96% alcohol, 10% formaldehyde and hair fixing spray sold in all pharmacies with different trademarks. The morphological parameters such as: integrity and cellular architecture that were analyzed with the hair fixer showed good results when compared to the other fixers that were already being used in cytological routines, making it useful as an alternative product in the fixations of exfoliative cytoglia. However, it is suggested that new color tests be performed for greater accuracy of the analyses