Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis

Detalhes bibliográficos
Autor(a) principal: Calixto, Christiano
Data de Publicação: 2021
Outros Autores: Silva, Jhenifer N., Sampaio, Angélica Fernandes Arcanjo, Nogueira, Cintia Lopes, Abreu, Leonardo Araujo de, Oliveira, Pedro Lagerblad de, Gondim, Katia Calp, Moraes, Bruno, Carvalho, Stephanie Serafim de, Silva, Renato Martins da, Vaz Junior, Itabajara da Silva, Moreira, Luciano A., Logullo, Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/231311
Resumo: In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions.
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spelling Calixto, ChristianoSilva, Jhenifer N.Sampaio, Angélica Fernandes ArcanjoNogueira, Cintia LopesAbreu, Leonardo Araujo deOliveira, Pedro Lagerblad deGondim, Katia CalpMoraes, BrunoCarvalho, Stephanie Serafim deSilva, Renato Martins daVaz Junior, Itabajara da SilvaMoreira, Luciano A.Logullo, Carlos2021-10-27T04:26:19Z20212045-2322http://hdl.handle.net/10183/231311001132115In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions.application/pdfengScientific reports. London. Vol. 11 (2021), 19202, 12 p.WolbachiaMetabolismo energéticoRespostas imunesAedes fluviatilisLinhagem celularInterações hospedeiro-patógenoAedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosisEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001132115.pdf.txt001132115.pdf.txtExtracted Texttext/plain56963http://www.lume.ufrgs.br/bitstream/10183/231311/2/001132115.pdf.txt7a2923de2c751d5b827fb17753d4af53MD52ORIGINAL001132115.pdfTexto completo (inglês)application/pdf6780691http://www.lume.ufrgs.br/bitstream/10183/231311/1/001132115.pdff789186371d612751ecec25604c253f6MD5110183/2313112024-10-02 06:39:37.681757oai:www.lume.ufrgs.br:10183/231311Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-10-02T09:39:37Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
title Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
spellingShingle Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
Calixto, Christiano
Wolbachia
Metabolismo energético
Respostas imunes
Aedes fluviatilis
Linhagem celular
Interações hospedeiro-patógeno
title_short Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
title_full Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
title_fullStr Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
title_full_unstemmed Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
title_sort Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
author Calixto, Christiano
author_facet Calixto, Christiano
Silva, Jhenifer N.
Sampaio, Angélica Fernandes Arcanjo
Nogueira, Cintia Lopes
Abreu, Leonardo Araujo de
Oliveira, Pedro Lagerblad de
Gondim, Katia Calp
Moraes, Bruno
Carvalho, Stephanie Serafim de
Silva, Renato Martins da
Vaz Junior, Itabajara da Silva
Moreira, Luciano A.
Logullo, Carlos
author_role author
author2 Silva, Jhenifer N.
Sampaio, Angélica Fernandes Arcanjo
Nogueira, Cintia Lopes
Abreu, Leonardo Araujo de
Oliveira, Pedro Lagerblad de
Gondim, Katia Calp
Moraes, Bruno
Carvalho, Stephanie Serafim de
Silva, Renato Martins da
Vaz Junior, Itabajara da Silva
Moreira, Luciano A.
Logullo, Carlos
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Calixto, Christiano
Silva, Jhenifer N.
Sampaio, Angélica Fernandes Arcanjo
Nogueira, Cintia Lopes
Abreu, Leonardo Araujo de
Oliveira, Pedro Lagerblad de
Gondim, Katia Calp
Moraes, Bruno
Carvalho, Stephanie Serafim de
Silva, Renato Martins da
Vaz Junior, Itabajara da Silva
Moreira, Luciano A.
Logullo, Carlos
dc.subject.por.fl_str_mv Wolbachia
Metabolismo energético
Respostas imunes
Aedes fluviatilis
Linhagem celular
Interações hospedeiro-patógeno
topic Wolbachia
Metabolismo energético
Respostas imunes
Aedes fluviatilis
Linhagem celular
Interações hospedeiro-patógeno
description In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-10-27T04:26:19Z
dc.date.issued.fl_str_mv 2021
dc.type.driver.fl_str_mv Estrangeiro
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dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/231311
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.nrb.pt_BR.fl_str_mv 001132115
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url http://hdl.handle.net/10183/231311
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Scientific reports. London. Vol. 11 (2021), 19202, 12 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
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