Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/231311 |
Resumo: | In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions. |
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Calixto, ChristianoSilva, Jhenifer N.Sampaio, Angélica Fernandes ArcanjoNogueira, Cintia LopesAbreu, Leonardo Araujo deOliveira, Pedro Lagerblad deGondim, Katia CalpMoraes, BrunoCarvalho, Stephanie Serafim deSilva, Renato Martins daVaz Junior, Itabajara da SilvaMoreira, Luciano A.Logullo, Carlos2021-10-27T04:26:19Z20212045-2322http://hdl.handle.net/10183/231311001132115In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions.application/pdfengScientific reports. London. Vol. 11 (2021), 19202, 12 p.WolbachiaMetabolismo energéticoRespostas imunesAedes fluviatilisLinhagem celularInterações hospedeiro-patógenoAedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosisEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001132115.pdf.txt001132115.pdf.txtExtracted Texttext/plain56963http://www.lume.ufrgs.br/bitstream/10183/231311/2/001132115.pdf.txt7a2923de2c751d5b827fb17753d4af53MD52ORIGINAL001132115.pdfTexto completo (inglês)application/pdf6780691http://www.lume.ufrgs.br/bitstream/10183/231311/1/001132115.pdff789186371d612751ecec25604c253f6MD5110183/2313112024-10-02 06:39:37.681757oai:www.lume.ufrgs.br:10183/231311Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-10-02T09:39:37Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
title |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
spellingShingle |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis Calixto, Christiano Wolbachia Metabolismo energético Respostas imunes Aedes fluviatilis Linhagem celular Interações hospedeiro-patógeno |
title_short |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
title_full |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
title_fullStr |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
title_full_unstemmed |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
title_sort |
Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis |
author |
Calixto, Christiano |
author_facet |
Calixto, Christiano Silva, Jhenifer N. Sampaio, Angélica Fernandes Arcanjo Nogueira, Cintia Lopes Abreu, Leonardo Araujo de Oliveira, Pedro Lagerblad de Gondim, Katia Calp Moraes, Bruno Carvalho, Stephanie Serafim de Silva, Renato Martins da Vaz Junior, Itabajara da Silva Moreira, Luciano A. Logullo, Carlos |
author_role |
author |
author2 |
Silva, Jhenifer N. Sampaio, Angélica Fernandes Arcanjo Nogueira, Cintia Lopes Abreu, Leonardo Araujo de Oliveira, Pedro Lagerblad de Gondim, Katia Calp Moraes, Bruno Carvalho, Stephanie Serafim de Silva, Renato Martins da Vaz Junior, Itabajara da Silva Moreira, Luciano A. Logullo, Carlos |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Calixto, Christiano Silva, Jhenifer N. Sampaio, Angélica Fernandes Arcanjo Nogueira, Cintia Lopes Abreu, Leonardo Araujo de Oliveira, Pedro Lagerblad de Gondim, Katia Calp Moraes, Bruno Carvalho, Stephanie Serafim de Silva, Renato Martins da Vaz Junior, Itabajara da Silva Moreira, Luciano A. Logullo, Carlos |
dc.subject.por.fl_str_mv |
Wolbachia Metabolismo energético Respostas imunes Aedes fluviatilis Linhagem celular Interações hospedeiro-patógeno |
topic |
Wolbachia Metabolismo energético Respostas imunes Aedes fluviatilis Linhagem celular Interações hospedeiro-patógeno |
description |
In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-10-27T04:26:19Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/231311 |
dc.identifier.issn.pt_BR.fl_str_mv |
2045-2322 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001132115 |
identifier_str_mv |
2045-2322 001132115 |
url |
http://hdl.handle.net/10183/231311 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Scientific reports. London. Vol. 11 (2021), 19202, 12 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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